LOCUS       sequence-275126-        2097 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CTAG Nuclear localiation signal SV40 for fusion with CDS12.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Sauret-Gueto S, Frangedakis E, Silvestri L, Rebmann M, Tomaselli M, 
            Markel K, Delmans M, West A, Patron NJ, Haseloff J
  TITLE     Systematic tools for reprogramming plant gene expression in a simple
            model, Marchantia polymorpha.
  JOURNAL   ACS Synth Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511.
  PUBMED    32163700
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511."
COMMENT     Sequence Label: CTAG_NLS-SV40
FEATURES             Location/Qualifiers
     source          1..2097
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     350..369
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     603..620
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(644..701)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     CDS             complement(728..748)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      774..817
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(821..839)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             1001..1660
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1067..1086)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1681..1775
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      join(1958..2097,1..449)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
       61 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
      121 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
      181 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
      241 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
      301 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
      361 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
      421 gctcgtcagg ggggcggagc ctgtggaaaa acgccagcaa cgcggccttt ttacggttcc
      481 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
      541 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
      601 gcagcgagtc agtgagcgag gaagcctgca taacgcgaag taatcttttc ggttttaaag
      661 aaaaagggca gggtggtgac accttgccct tttttgccgg actgcagggt ctcgaagctt
      721 atcctccaac ctttctcttc ttcttaggct gcaacgaatg agaccgaatt ccagaaatca
      781 tccttagcga aagctaagga ttttttttat ctgaaattct gcctcgtgat acgcctattt
      841 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac tcgagttgat
      901 cgggcacgta agaggttcca actttcacca taatgaaata agatcactac cgggcgtatt
      961 ttttgagtta tcgagatttt caggagctaa ggaagctaaa atggagaaaa aaatcactgg
     1021 atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg catttcagtc
     1081 agttgctcaa tgtacctata accagaccgt tcagctggat attacggcct ttttaaagac
     1141 cgtaaagaaa aataagcaca agttttatcc ggcctttatt cacattcttg cccgcctgat
     1201 gaatgctcat ccggaatttc gtatggcaat gaaagacggt gagctggtga tatgggatag
     1261 tgttcaccct tgttacaccg ttttccatga gcaaactgaa acgttttcat cgctctggag
     1321 tgaataccac gacgatttcc ggcagtttct acacatatat tcgcaagatg tggcgtgtta
     1381 cggtgaaaac ctggcctatt tccctaaagg gtttattgag aatatgtttt tcgtctcagc
     1441 caatccctgg gtgagtttca ccagttttga tttaaacgtg gccaatatgg acaacttctt
     1501 cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct
     1561 ggcgattcag gttcatcatg ccgtttgtga tggcttccat gtcggcagaa tgcttaatga
     1621 attacaacag tactgcgatg agtggcaggg cggggcgtaa tttgatatcg agctcgcttg
     1681 gactcctgtt gatagatcca gtaatgacct cagaactcca tctggatttg ttcagaacgc
     1741 tcggttgccg ccgggcgttt tttattggtg agaatccaag cctcgagctg tcagaccaag
     1801 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     1861 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     1921 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     1981 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     2041 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaa
//
