LOCUS       sequence-275127-        3853 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PROM for cloning with 5UTR_MpUBE2. PROM+5UTR_MpUBE2 has ubiquitous 
            expression in early satges of gemmae development..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3853)
  AUTHORS   Sauret-Gueto S, Frangedakis E, Silvestri L, Rebmann M, Tomaselli M, 
            Markel K, Delmans M, West A, Patron NJ, Haseloff J
  TITLE     Systematic tools for reprogramming plant gene expression in a simple
            model, Marchantia polymorpha.
  JOURNAL   ACS Synth Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511.
  PUBMED    32163700
REFERENCE   2  (bases 1 to 3853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511."
COMMENT     Sequence Label: PROM_MpUBE2
FEATURES             Location/Qualifiers
     source          1..3853
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      52..146
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      329..917
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     818..837
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1071..1088
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(1112..1169)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     CDS             complement(1516..1527)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      2998..3041
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(3045..3063)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             join(3225..3853,1..31)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(3291..3310)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 gtactgcgat gagtggcagg gcggggcgta atttgatatc gagctcgctt ggactcctgt
       61 tgatagatcc agtaatgacc tcagaactcc atctggattt gttcagaacg ctcggttgcc
      121 gccgggcgtt ttttattggt gagaatccaa gcctcgagct gtcagaccaa gtttactcat
      181 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
      241 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
      301 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
      361 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
      421 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
      481 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
      541 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
      601 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
      661 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
      721 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
      781 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
      841 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
      901 ggcggagcct gtggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
      961 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     1021 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     1081 tgagcgagga agcctgcata acgcgaagta atcttttcgg ttttaaagaa aaagggcagg
     1141 gtggtgacac cttgcccttt tttgccggac tgcagggtct cgagtaccga ccccgacttc
     1201 gatgtggacg gaatatgctc cgcccgcaca ccctcgctcc gtaccagttc aaagtcagtc
     1261 ctgctcccgg tcgtggccgt gaattgcagt cctcccttcc cattcgactg tcggatccgc
     1321 acccgcatcc gcatccgccg gtcaaatcag aaccctcttc tcgcccgcca tccagcgcga
     1381 cgaagcttcc ggcgtctgac gctgcatttc gcgtttcacg gctctgaatc tccggacgca
     1441 aagtccctcc accttcggat tcgggatcgg acggttggcc cggttatgtc ccgattccgc
     1501 tctctgtcct cctcctcggc cttcgatacc acgtacgtcc cccgagtttc tggcgtggac
     1561 ttcatccgtc agtgcttcgg gctctccggc tcggtggatc tcgaatccga cggccacgcg
     1621 gcatggcccc ggcggatggc atgccgcccc gctgccgttt gcgtcatgtt acacggtcga
     1681 ggcacgtttt gccgttcctc gcagccttcc ccgtgacaca tccgcactcg gcacccagaa
     1741 tcactggctc catccatcct tccatccatc catccatcgc gacaggcgac ggcaacttta
     1801 tccacacatc cgagccagct accgttctgt cctcaatggg gaccggaaca cccggtccgg
     1861 tggcactcca cccgatctcc tgcgggactc tcttctatcc gcaacaagta agccgctacg
     1921 ccagctacct ccaggctaca agttccacgc gttttggtgc tggtaacttc cgggcaccga
     1981 accgaagctg cacgccaagc tcggacagct actagaccta aatccccgag gctgtctgga
     2041 acaatctggt aaatgctgtt ccggtcgcgc catcggcccg acgggcggat aatcaatgac
     2101 gaagtgtccg acgcggcaca gctgggccga cgggagccat gcgtttggtt tcgccctcgt
     2161 gagtggggcg ctgaggctcg gcacgctctg gggccacagt cctgggtcgt gtagctattt
     2221 aatcctgctc cgaaattccc ttccagatgg acggacattc agagggtcag tcggtcagtc
     2281 gccccgatct agctagcccc ggtccccgtc gatcctggcc gcagcgacag tccacttgtt
     2341 cgaccctttc agcgctcgtc gttcccttcg ttcgtgcctt gcggaggact gcagagactc
     2401 acgtccggcc tcgtcgtccc gtcgctcggg gccttgcttc tccacgctac gcgacgcgtc
     2461 acccccgtcg tcccagttct tcccggccct cgtcgtcctc tttcgggccc cgactgccgc
     2521 acctgccggt ccgtccgccc ggtttcgacg gtccccatcc cgctgccggt agcatgtcca
     2581 cacctacgat cgcctggttc gagcctatgg ccagaaatgc tattctcacg ttcgagtccg
     2641 gtgaccaccc ccggcccacc gaatgaccca tgtgccatca tggccgtgcc gggccttgca
     2701 gggaagtccg gattacgcaa tgatttccga agccaggggt cgccgacttt gggcttccac
     2761 gtcctgcgac tcgattccct tgtcatgacc gcaccgcggc cttctccccg ctcggccccc
     2821 aacctgagtt caggctgtcg gaataatgcc atgcacgttt ccccccgcac ggtaaacact
     2881 gtacccgaga tctatccgca aggaggtttt gggttgggcg gtttcgcatt tagtggttgg
     2941 agggctggaa acaccgccaa aatttttatc cgccccgtct cctgagaccg aattccagaa
     3001 atcatcctta gcgaaagcta aggatttttt ttatctgaaa ttctgcctcg tgatacgcct
     3061 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcactcgagt
     3121 tgatcgggca cgtaagaggt tccaactttc accataatga aataagatca ctaccgggcg
     3181 tattttttga gttatcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca
     3241 ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc
     3301 agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa
     3361 agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc
     3421 tgatgaatgc tcatccggaa tttcgtatgg caatgaaaga cggtgagctg gtgatatggg
     3481 atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct
     3541 ggagtgaata ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt
     3601 gttacggtga aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct
     3661 cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact
     3721 tcttcgcccc cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc
     3781 cgctggcgat tcaggttcat catgccgttt gtgatggctt ccatgtcggc agaatgctta
     3841 atgaattaca aca
//
