LOCUS       sequence-275129-        2923 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Strong promoter that drives expression everywhere in Mp gemmae but 
            much weaker in the notches.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Sauret-Gueto S, Frangedakis E, Silvestri L, Rebmann M, Tomaselli M, 
            Markel K, Delmans M, West A, Patron NJ, Haseloff J
  TITLE     Systematic tools for reprogramming plant gene expression in a simple
            model, Marchantia polymorpha.
  JOURNAL   ACS Synth Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511.
  PUBMED    32163700
REFERENCE   2  (bases 1 to 2923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511."
COMMENT     Sequence Label: PROM5_35S
FEATURES             Location/Qualifiers
     source          1..2923
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     357..376
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     610..627
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(651..708)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     promoter        complement(755..1100)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(792..812)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     terminator      1607..1650
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(1654..1672)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             1834..2493
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1900..1919)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      2514..2608
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      join(2791..2923,1..456)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
       61 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
      121 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
      181 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
      241 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
      301 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
      361 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
      421 ttgtgatgct cgtcaggggg gcggagcctg tggaaaaacg ccagcaacgc ggccttttta
      481 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
      541 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
      601 accgagcgca gcgagtcagt gagcgaggaa gcctgcataa cgcgaagtaa tcttttcggt
      661 tttaaagaaa aagggcaggg tggtgacacc ttgccctttt ttgccggact gcagggtctc
      721 acattggtga tgttggatcc tctagagtcc cccgtgttct ctccaaatga aatgaacttc
      781 cttatataga ggaagggtct tgcgaaggat agtgggattg tgcgtcatcc cttacgtcag
      841 tggagatatc acatcaatcc acttgctttg tagacgtggt tggaacgttt tctttttcca
      901 cgatgctcct cgtgggtggg ggtccatctt tgggaccact gtcggcagag gcatcttcaa
      961 cgatggcctt tcctttatcg caatgatggc atttgtagga gccaccttcc ttttccacta
     1021 tcttcacaat aaagtgacag atagctgggc aatggaatcc gaggaggttt ccggatatta
     1081 ccctttgttg aaaagtctca attgcccttt ggtctactga gactgtatct ttgatatttt
     1141 tggagtagac aagtgtgtcg tgctccacca tgttgacgaa gattttcttc ttgtcattga
     1201 gtcgtaagag actctgtatg aactgttcgc cagtctttac ggcgagttct gttaggtcct
     1261 ctatttgaat ctttgactcc atggcctttg attcagtggg aactaccttt ttagagactc
     1321 caatctctat tacttgcctt ggtttgtgaa gcaagccttg aatcgtccat actggaatag
     1381 tacttctgat cttgagaaat atatctttct ctgtgttctt gatgcagtta gtcctgaatc
     1441 ttttgactgc atctttaacc ttcttgggaa ggtatttgat ttcctgtaga ttattgctcg
     1501 ggtagatcgt cttgatgaga cttgctgcgt aagcctctct aaccatctgt gggttagcat
     1561 tctttctgaa attgaaaagg ctaatctctc ccgagaccga attccagaaa tcatccttag
     1621 cgaaagctaa ggattttttt tatctgaaat tctgcctcgt gatacgccta tttttatagg
     1681 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cactcgagtt gatcgggcac
     1741 gtaagaggtt ccaactttca ccataatgaa ataagatcac taccgggcgt attttttgag
     1801 ttatcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc
     1861 accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct
     1921 caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag
     1981 aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct
     2041 catccggaat ttcgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac
     2101 ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac
     2161 cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa
     2221 aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc
     2281 tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc
     2341 gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt
     2401 caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa
     2461 cagtactgcg atgagtggca gggcggggcg taatttgata tcgagctcgc ttggactcct
     2521 gttgatagat ccagtaatga cctcagaact ccatctggat ttgttcagaa cgctcggttg
     2581 ccgccgggcg ttttttattg gtgagaatcc aagcctcgag ctgtcagacc aagtttactc
     2641 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     2701 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     2761 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     2821 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     2881 accaactctt tttccgaagg taactggctt cagcagagcg cag
//
