LOCUS       sequence-275130-        2867 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Strong promoter that drives expression everywhere in Mp gemmae but 
            much weaker in the notches.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Sauret-Gueto S, Frangedakis E, Silvestri L, Rebmann M, Tomaselli M, 
            Markel K, Delmans M, West A, Patron NJ, Haseloff J
  TITLE     Systematic tools for reprogramming plant gene expression in a simple
            model, Marchantia polymorpha.
  JOURNAL   ACS Synth Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511.
  PUBMED    32163700
REFERENCE   2  (bases 1 to 2867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511."
COMMENT     Sequence Label: PROM5_35Sx2
FEATURES             Location/Qualifiers
     source          1..2867
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     313..333
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     terminator      433..490
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(514..531)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(685..1273)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(765..784)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1456..1550)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1571..2230)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2145..2164
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2392..2410
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2414..2457)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     promoter        join(2567..2867,1..376)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
ORIGIN
        1 agtctcagaa gaccaaaggg ctattgagac ttttcaacaa agggtaatat cgggaaacct
       61 cctcggattc cattgcccag ctatctgtca cttcatcaaa aggacagtag aaaaggaagg
      121 tggcacctac aaatgccatc attgcgataa aggaaaggct atcgttcaag atgcctctgc
      181 cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt
      241 tccaaccacg tcttcaaagc aagtggattg atgtgatatc tccactgacg taagggatga
      301 cgcacaatcc cactatcctt cgcaagaccc ttcctctata taaggaagtt catttcattt
      361 ggagaggaca cgctgaaatc accagtctct ctctacaaat ctatctctct cgagcaatgt
      421 gagaccctgc agtccggcaa aaaagggcaa ggtgtcacca ccctgccctt tttctttaaa
      481 accgaaaaga ttacttcgcg ttatgcaggc ttcctcgctc actgactcgc tgcgctcggt
      541 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
      601 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
      661 taaaaaggcc gcgttgctgg cgtttttcca caggctccgc ccccctgacg agcatcacaa
      721 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
      781 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
      841 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
      901 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
      961 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     1021 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     1081 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     1141 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     1201 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     1261 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     1321 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     1381 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     1441 cagctcgagg cttggattct caccaataaa aaacgcccgg cggcaaccga gcgttctgaa
     1501 caaatccaga tggagttctg aggtcattac tggatctatc aacaggagtc caagcgagct
     1561 cgatatcaaa ttacgccccg ccctgccact catcgcagta ctgttgtaat tcattaagca
     1621 ttctgccgac atggaagcca tcacaaacgg catgatgaac ctgaatcgcc agcggcatca
     1681 gcaccttgtc gccttgcgta taatatttgc ccatggtgaa aacgggggcg aagaagttgt
     1741 ccatattggc cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgagacga
     1801 aaaacatatt ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca
     1861 catcttgcga atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagcg
     1921 atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata
     1981 tcaccagctc accgtctttc attgccatac gaaattccgg atgagcattc atcaggcggg
     2041 caagaatgtg aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa
     2101 aggccgtaat atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg
     2161 cctcaaaatg ttctttacga tgccattggg atatatcaac ggtggtatat ccagtgattt
     2221 ttttctccat tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg
     2281 gtagtgatct tatttcatta tggtgaaagt tggaacctct tacgtgcccg atcaactcga
     2341 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
     2401 atcacgaggc agaatttcag ataaaaaaaa tccttagctt tcgctaagga tgatttctgg
     2461 aattcggtct ctggaggcta gagcagcttg ccaacatggt ggagcacgac actctcgtct
     2521 actccaagaa tatcaaagat acagtctcag aagaccaaag ggctattgag acttttcaac
     2581 aaagggtaat atcgggaaac ctcctcggat tccattgccc agctatctgt cacttcatca
     2641 aaaggacagt agaaaaggaa ggtggcacct acaaatgcca tcattgcgat aaaggaaagg
     2701 ctatcgttca agatgcctct gccgacagtg gtcccaaaga tggaccccca cccacgagga
     2761 gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat tgatgtgaac
     2821 atggtggagc acgacactct cgtctactcc aagaatatca aagatac
//
