LOCUS       sequence-275131-        3795 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Strong promoter from MpEF1a, that drives expression everywhere in Mp
            gemmae but stronger in the notches.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Sauret-Gueto S, Frangedakis E, Silvestri L, Rebmann M, Tomaselli M, 
            Markel K, Delmans M, West A, Patron NJ, Haseloff J
  TITLE     Systematic tools for reprogramming plant gene expression in a simple
            model, Marchantia polymorpha.
  JOURNAL   ACS Synth Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511.
  PUBMED    32163700
REFERENCE   2  (bases 1 to 3795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511."
COMMENT     Sequence Label: PROM5_MpEF1a
FEATURES             Location/Qualifiers
     source          1..3795
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1592..1649
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1673..1690)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1844..2432)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1924..1943)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2615..2709)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2730..3389)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     3304..3323
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     3551..3569
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(3573..3616)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
ORIGIN
        1 gtcttcgatt tttgattgtt aacgggtatc gatatttggg taacttaaaa agaatttgct
       61 aaagatctta aaaaacagat taaaaaaacc tttcaaaata gctaatcctt taaatccgtt
      121 tcacaatata atgctcattg atgataatcg aaaagattcc attttaaagt gcatataaat
      181 tgcttaatta tctctttctg tctcgtaata ttttgttctc tcctattgga tcggacccgc
      241 atcttctcga tcagttcact atactaatat ttggtcaact tcaagagaga ttagaacttc
      301 gttctccaaa gtgtaaaggc cgacaagcag aaaaggaaac ttcaatcaga agccttctag
      361 gttttcccgc gtcccaaaaa gcgaagggag gacggggagg acggtgaggc cagaagaaga
      421 aagcaacagt aaaatctcat gtgattgcgt gcatgtcatg gatctatggg atgctagtcc
      481 accgtttgga agtatgtgca gaaggagaaa ggcgcgggag gccaaaagcg acttcggcca
      541 cctctctact ttctagaaag tttgctcgca gcttgtaata agcgcggaaa aggtactcga
      601 aaaaccggag caatcggtgt ggtatcagca gtcgggttcg ctaaacctac gtcttcttat
      661 tttggaatgt taaattgaaa gggcgtgccg gccctaagca cggacgatgt gacagacgga
      721 ggagtgtgag caattcggat gacgatgctg gccggcagtc acgacaaaga gtccgaaggg
      781 gcatggacag ggcaatatat aaatggaaaa gaggctttga atattcctaa cccttcgtcg
      841 tcggcctccc aacttgccct cgcatgctga attggccgcc tatctgcgaa tggccggccg
      901 ccgaccctaa gtcgcaataa cttgaacgga gccgatgcaa agtccttatc gaacgctcgt
      961 tattataaac ggggcagacc taggtattta attatatcaa aaccctccgc aatttcgcta
     1021 atcattccct aatttcttct actttggcgt ctagtgctgt ggtcgcgctt gctctcaaag
     1081 ctcgctggga ggcagtggag cgtctggctt aaaatcgatc gccgcaaccc tagagttcct
     1141 ttcacttctt tctctcttgc gtggagagca tcgacatcga ggagctgcat cttgtcttct
     1201 ggtatgcgcc ttgtgccctc gtctctgtac gtcgttacga ttgctcgata ttgtgattgt
     1261 ctagacctga aacccgactg gagcttttac gtggtgtcga ttggggcctc gagcgagtga
     1321 ttttttaggt tcgttttagc caagtacatc ccactttggt cagtcctgtt atctcggtaa
     1381 ggtgttcagt tcggtcactg gtgcggaggt gcctcgtaag tttcctgcat gatagatttg
     1441 tctatccttg acttgaagag gagtcgaagg tcagcgaggt tggctgtgtg ctatggacgt
     1501 cggtgctcct tggttccgtg cttctatggg tgttgcctca acagtattga tgtgcctgca
     1561 gaaaggttgt caccaatgcg agaccctgca gtccggcaaa aaagggcaag gtgtcaccac
     1621 cctgcccttt ttctttaaaa ccgaaaagat tacttcgcgt tatgcaggct tcctcgctca
     1681 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     1741 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     1801 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccac aggctccgcc
     1861 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     1921 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     1981 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     2041 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     2101 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     2161 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     2221 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     2281 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     2341 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     2401 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     2461 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     2521 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     2581 atgagtaaac ttggtctgac agctcgaggc ttggattctc accaataaaa aacgcccggc
     2641 ggcaaccgag cgttctgaac aaatccagat ggagttctga ggtcattact ggatctatca
     2701 acaggagtcc aagcgagctc gatatcaaat tacgccccgc cctgccactc atcgcagtac
     2761 tgttgtaatt cattaagcat tctgccgaca tggaagccat cacaaacggc atgatgaacc
     2821 tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catggtgaaa
     2881 acgggggcga agaagttgtc catattggcc acgtttaaat caaaactggt gaaactcacc
     2941 cagggattgg ctgagacgaa aaacatattc tcaataaacc ctttagggaa ataggccagg
     3001 ttttcaccgt aacacgccac atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg
     3061 tggtattcac tccagagcga tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa
     3121 gggtgaacac tatcccatat caccagctca ccgtctttca ttgccatacg aaattccgga
     3181 tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt gtgcttattt
     3241 ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt ataggtacat
     3301 tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga tatatcaacg
     3361 gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga aaatctcgat
     3421 aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt ggaacctctt
     3481 acgtgcccga tcaactcgag tgccacctga cgtctaagaa accattatta tcatgacatt
     3541 aacctataaa aataggcgta tcacgaggca gaatttcaga taaaaaaaat ccttagcttt
     3601 cgctaaggat gatttctgga attcggtctc aggagcaaat gagtcacaca cattgttgag
     3661 agacatatca aaataactca attgtaaggt aagttgatag atttcccagt taaatgcatt
     3721 attttaacaa tatttgtatc tcaaattttc aaaatcatat caaatttgca gatcttgatg
     3781 atgattcaca actct
//
