LOCUS       sequence-275137-       10745 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Reporter assay.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10745)
  AUTHORS   Kawano S, Maruyama J, Nagashima S, Inami K, Qiu W, Iwasa H, Nakagawa
            K, Ishigami-Yuasa M, Kagechika H, Nishina H, Hata Y
  TITLE     A cell-based screening for TAZ activators identifies ethacridine, a 
            widely used antiseptic and abortifacient, as a compound that 
            promotes dephosphorylation of TAZ and inhibits adipogenesis in 
            C3H10T1/2 cells.
  JOURNAL   J Biochem. 2015 May 14. pii: mvv051.
  PUBMED    25979969
REFERENCE   2  (bases 1 to 10745)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biochem. 
            2015 May 14. pii: mvv051."
COMMENT     Sequence Label: pLL3.7-K122-FH-TAZ-TEAD-p-H2B-mCherry
FEATURES             Location/Qualifiers
     source          1..10745
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        65..368
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        369..572
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             631..654
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             661..678
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    join(1909..2483,2484..2486,2487..2495)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2076..2093)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2303..2322
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(2496..2498,2499..2501,2502..3215)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2496..2498,2499..2501,2502..3212)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2541..2562)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2802..2821)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3149..3170
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(3241..3274)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    3330..3918
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3383..3403)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3801..3812)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3921..3937)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             4447..4627
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(4689..5277)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4769..4788)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5448..6239,6240..6308))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6071..6090
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6309..6413)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6488..6507)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        6679..7058
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7060..7258
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             7276..7456
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7503..7628
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8125..8358
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8543..8587
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8885..9002
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(9061..9771)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(9339..9358)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     9608..9626
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      9768..9777
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(9790..10167)
                     /codon_start=1
                     /gene="HIST1H2BB"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPSKSAPAPKKGSKKAITKAQKKDGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAVTKYTSSK"
     misc_feature    10561..10652
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(10593..10612)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    10666..10714
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 gcattagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
       61 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
      121 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      181 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      241 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      301 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      361 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      421 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      481 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      541 gtgtacggtg ggaggtctat ataagcagag ctggtttagt gaaccgtcag atccgctagc
      601 agatctcgag atctgctagc cggggccatg gactacaaag acgatgacga caaggaattg
      661 caccatcatc atcatcatga attcaatccg gcctcggcgc cccctccgct cccgccgcct
      721 gggcagcaag tgatccacgt cacgcaggac ctagacacag acctcgaagc cctcttcaac
      781 tctgtcatga atccgaagcc tagctcgtgg cggaagaaga tcctgccgga gtctttcttt
      841 aaggagcctg attcgggctc gcactcgcgc cagtccagca ccgactcgtc gggcggccac
      901 ccggggcctc gactggctgg gggtgcccag catgtccgct cgcactcgtc gcccgcgtcc
      961 ctgcagctgg gcaccggcgc gggtgctgcg ggtagccccg cgcagcagca cgcgcacctc
     1021 cgccagcagt cctacgacgt gaccgacgag ctgccactgc ccccgggctg ggagatgacc
     1081 ttcacggcca ctggccagag gtacttcctc aatcacatag aaaaaatcac cacatggcaa
     1141 gaccctagga aggcgatgaa tcagcctctg aatcatatga acctccaccc tgccgtcagt
     1201 tccacaccag tgcctcagag gtccatggca gtatcccagc caaatctcgt gatgaatcac
     1261 caacaccagc agcagatggc ccccagtacc ctgagccagc agaaccaccc cactcagaac
     1321 ccacccgcag ggctcatgag tatgcccaat gcgctgacca ctcagcagca gcagcagcag
     1381 aaactgcggc ttcagagaat ccagatggag agagaaagga ttcgaatgcg ccaagaggag
     1441 ctcatgaggc aggaagctgc cctctgtcga cagctcccca tggaagctga gactcttgcc
     1501 ccagttcagg ctgctgtcaa cccacccacg atgaccccag acatgagatc catcactaat
     1561 aatagctcag atcctttcct caatggaggg ccatatcatt cgagggagca gagcactgac
     1621 agtggcctgg ggttagggtg ctacagtgtc cccacaactc cggaggactt cctcagcaat
     1681 gtggatgaga tggatacagg agaaaacgca ggacaaacac ccatgaacat caatccccaa
     1741 cagacccgtt tccctgattt ccttgactgt cttccaggaa caaacgttga cttaggaact
     1801 ttggaatctg aagacctgat ccccctcttc aatgatgtag agtctgctct gaacaaaagt
     1861 gagccctttc taacctggct gtaactcgac ccgggcggcc gggatccgcc cctctccctc
     1921 ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta
     1981 tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc
     2041 tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct
     2101 gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt
     2161 agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa
     2221 gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg
     2281 gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga
     2341 tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtaca catgctttac
     2401 atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt
     2461 cctttgaaaa acacgatgat aatatggcca caaccatggt gagcaagggc gaggagctgt
     2521 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
     2581 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
     2641 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg
     2701 tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
     2761 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
     2821 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
     2881 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
     2941 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc
     3001 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca
     3061 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga
     3121 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
     3181 ggatcactct cggcatggac gagctgtaca agtaaagcgg ccaattcgtc gagggaccta
     3241 ataacttcgt atagcataca ttatacgaag ttatacatgt ttaagggttc cggttccact
     3301 aggtacaatt cgatatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa
     3361 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     3421 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     3481 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     3541 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     3601 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     3661 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     3721 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     3781 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     3841 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     3901 tttgggccgc ctccccgcat cgataccgtc gacctcgatc gagacctaga aaaacatgga
     3961 gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa
     4021 gaggaggagg aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac
     4081 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt
     4141 cactcccaac gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc
     4201 cctgattggc agaactacac accagggcca gggatcagat atccactgac ctttggatgg
     4261 tgctacaagc tagtaccagt tgagcaagag aaggtagaag aagccaatga aggagagaac
     4321 acccgcttgt tacaccctgt gagcctgcat gggatggatg acccggagag agaagtatta
     4381 gagtggaggt ttgacagccg cctagcattt catcacatgg cccgagagct gcatccggac
     4441 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     4501 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     4561 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     4621 tctagcagca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     4681 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     4741 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     4801 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     4861 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     4921 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     4981 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     5041 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     5101 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     5161 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     5221 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     5281 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     5341 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     5401 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     5461 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     5521 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     5581 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     5641 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     5701 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     5761 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     5821 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     5881 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     5941 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     6001 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     6061 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     6121 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     6181 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     6241 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     6301 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     6361 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     6421 ccccgaaaag tgccacctga cgtcgacgga tcgggagatc tcccgatccc ctatggtgca
     6481 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg
     6541 tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga
     6601 ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac
     6661 gggccagata tacgcgttga cattgattat tgactagtta ttaatagtaa tcaattacgg
     6721 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
     6781 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
     6841 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
     6901 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
     6961 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
     7021 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
     7081 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
     7141 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
     7201 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagcg
     7261 cgttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     7321 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     7381 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     7441 tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg
     7501 agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc
     7561 gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg
     7621 agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc
     7681 cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac
     7741 gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac
     7801 agctacaacc atcccttcag acaggatcag aagaacttag atcattatat aatacagtag
     7861 caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca
     7921 agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc ggccgctgat
     7981 cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa
     8041 agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca
     8101 gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg
     8161 aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg
     8221 tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca
     8281 actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct
     8341 aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc
     8401 tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac
     8461 ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga
     8521 agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc
     8581 aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat
     8641 gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag
     8701 agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc
     8761 cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg
     8821 attagtgaac ggatcggcac tgcgtgcgcc aattctgcag acaaatggca gtattcatcc
     8881 acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
     8941 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt
     9001 ttcgggttta ttacagggac agcagagatc cagtttggtt agtaccgggc ccgctctaga
     9061 ttacttgtac agctcgtcca tgccgccggt ggagtggcgg ccctcggcgc gttcgtactg
     9121 ttccacgatg gtgtagtcct cgttgtggga ggtgatgtcc aacttgatgt tgacgttgta
     9181 ggcgccgggc agctgcacgg gcttcttggc cttgtaggtg gtcttgacct cagcgtcgta
     9241 gtggccgccg tccttcagct tcagcctctg cttgatctcg cccttcaggg cgccgtcctc
     9301 ggggtacatc cgctcggagg aggcctccca gcccatggtc ttcttctgca ttacggggcc
     9361 gtcggagggg aagttggtgc cgcgcagctt caccttgtag atgaactcgc cgtcctgcag
     9421 ggaggagtcc tgggtcacgg tcaccacgcc gccgtcctcg aagttcatca cgcgctccca
     9481 cttgaagccc tcggggaagg acagcttcaa gtagtcgggg atgtcggcgg ggtgcttcac
     9541 gtaggccttg gagccgtaca tgaactgagg ggacaggatg tcccaggcga agggcagggg
     9601 gccacccttg gtcaccttca gcttggcggt ctgggtgccc tcgtaggggc ggccctcgcc
     9661 ctcgccctcg atctcgaact cgtggccgtt cacggagccc tccatgtgca ccttgaagcg
     9721 catgaactcc ttgatgatgg ccatgttatc ctcctcgccc ttgctcacca tggtggcgac
     9781 cggtggatcc ttagagctag tgtacttggt aactgcctta gtgccctcgg acacagcatg
     9841 cttagccagc tccccaggca gcagcaggcg cacagccgtc tgaatctccc tggaggtgat
     9901 ggtcgagcgc ttattgtagt gagccaggcg agaagcctcg cccgcgatgc gctcgaagat
     9961 gtcgttgacg aaggagttca tgatccccat ggccttggat gagatgccgg tgtcggggtg
    10021 gacctgcttc agaaccttgt acacatagat agaatagctc tccttgcggc tgcgcttacg
    10081 cttcttacca tccttcttct gcgccttagt gatagccttc ttagaaccct ttttaggggc
    10141 tggagcagac ttagagggtt caggcatggt ggcggtacca agcttggctg cccgcacagc
    10201 cctgctcacc gtctccgcag ctcagccctg gctggtcgct ccgtgctctc cggtctcaca
    10261 gccccgcttt tacgtgcccc ggagcctggg ctggccctgc aggtcgacgg tatcgataag
    10321 cttgatggac acacattcca cagctgggga cacacattcc acagctgggg acacacattc
    10381 cacagctggg gacacacatt ccacagctgg ggacacacat tccacagctg gggacacaca
    10441 ttccacagct ggggacacac attccacagc tggggacaca cattccacag catcgaattc
    10501 ctgcagcccg ggggatctcg agcccgggct agcacgcgta agagctcggt acctatcgat
    10561 agagaaatgt tctggcacct gcacttgcac tggggacagc ctattttgct agtttgtttt
    10621 gtttcgtttt gttttgatgg agagcgtatg ttagtactat cgattcacac aaaaaaccaa
    10681 cacacagatg taatgaaaat aaagatattt tattgcggcc gcgtggataa ccgtattacc
    10741 gccat
//
