LOCUS       sequence-27514-e         614 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Labarca C, Nowak MW, Zhang H, Tang L, Deshpande P, Lester HA
  TITLE     Channel gating governed symmetrically by conserved leucine residues 
            in the M2 domain of nicotinic receptors.
  JOURNAL   Nature. 1995 Aug 10. 376(6540):514-6.
  PUBMED    7637783
REFERENCE   2  (bases 1 to 614)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            1995 Aug 10. 376(6540):514-6."
COMMENT     Sequence Label: pBS-AMVpl
FEATURES             Location/Qualifiers
     source          1..614
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     86..102
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     86..102
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(144..160)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(145..161)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(240..258)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(279..295)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(279..295)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(292..314)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    303..319
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(327..333,334..351,352..357))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     complement(510..527)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gactcactat agggcgaatt ggagctcgtt tttattttta attttctttc aaatacttcc
       61 accatggcca ccgcggtggc ggccgtctag aactagtgga tcccccgggc tgcaggaatt
      121 cgatcagatc tgatcaagct tatcgatacc gtcgacctcg agggggggcc caaaaaaaaa
      181 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaagcggcc gcggtaccag cttttgttcc
      241 ctttagtgag ggttaatttc gagcttggcg taatcatggt catagctgtt tcctgtgtga
      301 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
      361 tggggtgcct aatgagtgag ctaactaaca ttaattgcgt tgcgctcact gcccgctttc
      421 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
      481 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
      541 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
      601 ggggataacg cagg
//
