LOCUS       sequence-275141-       10715 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes a Halo-labeled fusion of nuclease deficient Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10715)
  AUTHORS   Deng W, Shi X, Tjian R, Lionnet T, Singer RH
  TITLE     CASFISH: CRISPR/Cas9-mediated in situ labeling of genomic loci in 
            fixed cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Sep 22;112(38):11870-5. doi: 
            10.1073/pnas.1515692112. Epub 2015 Aug 31.
  PUBMED    26324940
REFERENCE   2  (bases 1 to 10715)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1515692112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-09-22- 22"; volume: "112"; issue: "38"; pages: "11870-5"
COMMENT     Sequence Label: pET302-6His-dCas9-Halo
FEATURES             Location/Qualifiers
     source          1..10715
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     62..81
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        62..80
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    81..105
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             153..170
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             join(174..197,198..200,201..2687,2688..2690,2691..4274)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4290..5177
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="AEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSY
                     VWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIH
                     DWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIID
                     QNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVE
                     EYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLI
                     GSEIARWLSTLEISG"
     primer_bind     complement(5269..5287)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      5283..5330
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(5568..5586)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5654..5758
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5759..5827,5828..6619)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5977..5996)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6790..7378
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7279..7298
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7532..7549
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7564..7703
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7629..7648)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7789..7811
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(7805..7996)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(9308..10390)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     10352..10371
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(10391..10468)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     10674..10693
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa
       61 ttaatacgac tcactatagg ggaattgtga gcggataaca attcccctct agaaataatt
      121 ttgtttaaac tttaagaagg agatatacta tgcatcatca tcatcatcac gtggataaga
      181 aatactcaat aggcttagct atcggcacaa atagcgtcgg atgggcggtg atcactgatg
      241 aatataaggt tccgtctaaa aagttcaagg ttctgggaaa tacagaccgc cacagtatca
      301 aaaaaaatct tataggggct cttttatttg acagtggaga gacagcggaa gcgactcgtc
      361 tcaaacggac agctcgtaga aggtatacac gtcggaagaa tcgtatttgt tatctacagg
      421 agattttttc aaatgagatg gcgaaagtag atgatagttt ctttcatcga cttgaagagt
      481 cttttttggt ggaagaagac aagaagcatg aacgtcatcc tatttttgga aatatagtag
      541 atgaagttgc ttatcatgag aaatatccaa ctatctatca tctgcgaaaa aaattggtag
      601 attctactga taaagcggat ttgcgcttaa tctatttggc cttagcgcat atgattaagt
      661 ttcgtggtca ttttttgatt gagggagatt taaatcctga taatagtgat gtggacaaac
      721 tatttatcca gttggtacaa acctacaatc aattatttga agaaaaccct attaacgcaa
      781 gtggagtaga tgctaaagcg attctttctg cacgattgag taaatcaaga cgattagaaa
      841 atctcattgc tcagctcccc ggtgagaaga aaaatggctt atttgggaat ctcattgctt
      901 tgtcattggg tttgacccct aattttaaat caaattttga tttggcagaa gatgctaaat
      961 tacagctttc aaaagatact tacgatgatg atttagataa tttattggcg caaattggag
     1021 atcaatatgc tgatttgttt ttggcagcta agaatttatc agatgctatt ttactttcag
     1081 atatcctaag agtaaatact gaaataacta aggctcccct atcagcttca atgattaaac
     1141 gctacgatga acatcatcaa gacttgactc ttttaaaagc tttagttcga caacaacttc
     1201 cagaaaagta taaagaaatc ttttttgatc aatcaaaaaa cggatatgca ggttatattg
     1261 atgggggagc tagccaagaa gaattttata aatttatcaa accaatttta gaaaaaatgg
     1321 atggtactga ggaattattg gtgaaactaa atcgtgaaga tttgctgcgc aagcaacgga
     1381 cctttgacaa cggctctatt ccccatcaaa ttcacttggg tgagctgcat gctattttga
     1441 gaagacaaga agacttttat ccatttttaa aagacaatcg tgagaagatt gaaaaaatct
     1501 tgacttttcg aattccttat tatgttggtc cattggcgcg tggcaatagt cgttttgcat
     1561 ggatgactcg gaagtctgaa gaaacaatta ccccatggaa ttttgaagaa gttgtcgata
     1621 aaggtgcttc agctcaatca tttattgaac gcatgacaaa ctttgataaa aatcttccaa
     1681 atgaaaaagt actaccaaaa catagtttgc tttatgagta ttttacggtt tataacgaat
     1741 tgacaaaggt caaatatgtt actgaaggaa tgcgaaaacc agcatttctt tcaggtgaac
     1801 agaagaaagc cattgttgat ttactcttca aaacaaatcg aaaagtaacc gttaagcaat
     1861 taaaagaaga ttatttcaaa aaaatagaat gttttgatag tgttgaaatt tcaggagttg
     1921 aagatagatt taatgcttca ttaggtacct accatgattt gctaaaaatt attaaagata
     1981 aagatttttt ggataatgaa gaaaatgaag atatcttaga ggatattgtt ttaacattga
     2041 ccttatttga agatagggag atgattgagg aaagacttaa aacatatgct cacctctttg
     2101 atgataaggt gatgaaacag cttaaacgtc gccgttatac tggttgggga cgtttgtctc
     2161 gaaaattgat taatggtatt agggataagc aatctggcaa aacaatatta gattttttga
     2221 aatcagatgg ttttgccaat cgcaatttta tgcagctgat ccatgatgat agtttgacat
     2281 ttaaagaaga cattcaaaaa gcacaagtgt ctggacaagg cgatagttta catgaacata
     2341 ttgcaaattt agctggtagc cctgctatta aaaaaggtat tttacagact gtaaaagttg
     2401 ttgatgaatt ggtcaaagta atggggcggc ataagccaga aaatatcgtt attgaaatgg
     2461 cacgtgaaaa tcagacaact caaaagggcc agaaaaattc gcgagagcgt atgaaacgaa
     2521 tcgaagaagg tatcaaagaa ttaggaagtc agattcttaa agagcatcct gttgaaaata
     2581 ctcaattgca aaatgaaaag ctctatctct attatctcca aaatggaaga gacatgtatg
     2641 tggaccaaga attagatatt aatcgtttaa gtgattatga tgtcgatgcc attgttccac
     2701 aaagtttcct taaagacgat tcaatagaca ataaggtctt aacgcgttct gataaaaatc
     2761 gtggtaaatc ggataacgtt ccaagtgaag aagtagtcaa aaagatgaaa aactattgga
     2821 gacaacttct aaacgccaag ttaatcactc aacgtaagtt tgataattta acgaaagctg
     2881 aacgtggagg tttgagtgaa cttgataaag ctggttttat caaacgccaa ttggttgaaa
     2941 ctcgccaaat cactaagcat gtggcacaaa ttttggatag tcgcatgaat actaaatacg
     3001 atgaaaatga taaacttatt cgagaggtta aagtgattac cttaaaatct aaattagttt
     3061 ctgacttccg aaaagatttc caattctata aagtacgtga gattaacaat taccatcatg
     3121 cccatgatgc gtatctaaat gccgtcgttg gaactgcttt gattaagaaa tatccaaaac
     3181 ttgaatcgga gtttgtctat ggtgattata aagtttatga tgttcgtaaa atgattgcta
     3241 agtctgagca agaaataggc aaagcaaccg caaaatattt cttttactct aatatcatga
     3301 acttcttcaa aacagaaatt acacttgcaa atggagagat tcgcaaacgc cctctaatcg
     3361 aaactaatgg ggaaactgga gaaattgtct gggataaagg gcgagatttt gccacagtgc
     3421 gcaaagtatt gtccatgccc caagtcaata ttgtcaagaa aacagaagta cagacaggcg
     3481 gattctccaa ggagtcaatt ttaccaaaaa gaaattcgga caagcttatt gctcgtaaaa
     3541 aagactggga tccaaaaaaa tatggtggtt ttgatagtcc aacggtagct tattcagtcc
     3601 tagtggttgc taaggtggaa aaagggaaat cgaagaagtt aaaatccgtt aaagagttac
     3661 tagggatcac aattatggaa agaagttcct ttgaaaaaaa tccgattgac tttttagaag
     3721 ctaaaggata taaggaagtt aaaaaagact taatcattaa actacctaaa tatagtcttt
     3781 ttgagttaga aaacggtcgt aaacggatgc tggctagtgc cggagaatta caaaaaggaa
     3841 atgagctggc tctgccaagc aaatatgtga attttttata tttagctagt cattatgaaa
     3901 agttgaaggg tagtccagaa gataacgaac aaaaacaatt gtttgtggag cagcataagc
     3961 attatttaga tgagattatt gagcaaatca gtgaattttc taagcgtgtt attttagcag
     4021 atgccaattt agataaagtt cttagtgcat ataacaaaca tagagacaaa ccaatacgtg
     4081 aacaagcaga aaatattatt catttattta cgttgacgaa tcttggagct cccgctgctt
     4141 ttaaatattt tgatacaaca attgatcgta aacgatatac gtctacaaaa gaagttttag
     4201 atgccactct tatccatcaa tccatcactg gtctttatga aacacgcatt gatttgagtc
     4261 agctaggagg tgacggtggc tccagatctg cagaaatcgg tactggcttt ccattcgacc
     4321 cccattatgt ggaagtcctg ggcgagcgca tgcactacgt cgatgttggt ccgcgcgatg
     4381 gcacccctgt gctgttcctg cacggtaacc cgacctcctc ctacgtgtgg cgcaacatca
     4441 tcccgcatgt tgcaccgacc catcgctgca ttgctccaga cctgatcggt atgggcaaat
     4501 ccgacaaacc agacctgggt tatttcttcg acgaccacgt ccgcttcatg gatgccttca
     4561 tcgaagccct gggtctggaa gaggtcgtcc tggtcattca cgactggggc tccgctctgg
     4621 gtttccactg ggccaagcgc aatccagagc gcgtcaaagg tattgcattt atggagttca
     4681 tccgccctat cccgacctgg gacgaatggc cagaatttgc ccgcgagacc ttccaggcct
     4741 tccgcaccac cgacgtcggc cgcaagctga tcatcgatca gaacgttttt atcgagggta
     4801 cgctgccgat gggtgtcgtc cgcccgctga ctgaagtcga gatggaccat taccgcgagc
     4861 cgttcctgaa tcctgttgac cgcgagccac tgtggcgctt cccaaacgag ctgccaatcg
     4921 ccggtgagcc agcgaacatc gtcgcgctgg tcgaagaata catggactgg ctgcaccagt
     4981 cccctgtccc gaagctgctg ttctggggca ccccaggcgt tctgatccca ccggccgaag
     5041 ccgctcgcct ggccaaaagc ctgcctaact gcaaggctgt ggacatcggc ccgggtctga
     5101 atctgctgca agaagacaac ccggacctga tcggcagcga gatcgcgcgc tggctgtcta
     5161 ctctggagat ttccggttaa tagctcgaga tcgatgatat tcgagcctag gtataatcgg
     5221 atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat
     5281 aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag
     5341 gaactatatc cggatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc
     5401 tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca
     5461 cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gctagcttat
     5521 cgatgataag ctgtcaaaca tgagaattaa ttcttgaaga cgaaagggcc tcgtgatacg
     5581 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     5641 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     5701 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     5761 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     5821 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     5881 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     5941 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     6001 tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     6061 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     6121 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     6181 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     6241 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     6301 tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     6361 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     6421 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     6481 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     6541 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     6601 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     6661 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     6721 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     6781 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     6841 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     6901 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     6961 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     7021 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     7081 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     7141 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     7201 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     7261 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     7321 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     7381 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     7441 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     7501 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     7561 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcaatggtgc
     7621 actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc
     7681 tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac
     7741 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     7801 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat
     7861 cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga
     7921 gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt
     7981 tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga
     8041 taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt
     8101 tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa
     8161 tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc
     8221 agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt
     8281 ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg
     8341 ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag
     8401 taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc
     8461 gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg
     8521 cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat
     8581 tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt
     8641 cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc
     8701 ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt
     8761 gacgatcagc ggtccaatga tcgaagttag gctggtaaga gccgcgagcg atccttgaag
     8821 ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc
     8881 gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa
     8941 cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc
     9001 gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc
     9061 gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa
     9121 aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat
     9181 aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc
     9241 tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta cattaattgc
     9301 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     9361 cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca
     9421 ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca
     9481 agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg
     9541 ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagata tccgcaccaa
     9601 cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa
     9661 ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg
     9721 acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat
     9781 atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca
     9841 gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtctt
     9901 catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg
     9961 gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa
    10021 tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga
    10081 cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt
    10141 taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa
    10201 tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct
    10261 ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca
    10321 ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta
    10381 ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc
    10441 gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc
    10501 tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat
    10561 ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc
    10621 acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg
    10681 tcggcgatat aggcgccagc aaccgcacct gtggc
//
