LOCUS       sequence-275152-        2265 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3TERM after CDS/ CDS12-CTAG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Sauret-Gueto S, Frangedakis E, Silvestri L, Rebmann M, Tomaselli M, 
            Markel K, Delmans M, West A, Patron NJ, Haseloff J
  TITLE     Systematic tools for reprogramming plant gene expression in a simple
            model, Marchantia polymorpha.
  JOURNAL   ACS Synth Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511.
  PUBMED    32163700
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511."
COMMENT     Sequence Label: 3TERM_35S
FEATURES             Location/Qualifiers
     source          1..2265
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(519..613)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(634..1293)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1208..1227
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1455..1473
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1477..1520)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     polyA_signal    1570..1744
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     terminator      1761..1818
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1842..1859)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(2013..2265,1..336))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2093..2112)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
       61 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
      121 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
      181 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
      241 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
      301 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
      361 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
      421 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
      481 atatatgagt aaacttggtc tgacagctcg aggcttggat tctcaccaat aaaaaacgcc
      541 cggcggcaac cgagcgttct gaacaaatcc agatggagtt ctgaggtcat tactggatct
      601 atcaacagga gtccaagcga gctcgatatc aaattacgcc ccgccctgcc actcatcgca
      661 gtactgttgt aattcattaa gcattctgcc gacatggaag ccatcacaaa cggcatgatg
      721 aacctgaatc gccagcggca tcagcacctt gtcgccttgc gtataatatt tgcccatggt
      781 gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac tggtgaaact
      841 cacccaggga ttggctgaga cgaaaaacat attctcaata aaccctttag ggaaataggc
      901 caggttttca ccgtaacacg ccacatcttg cgaatatatg tgtagaaact gccggaaatc
      961 gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta
     1021 acaagggtga acactatccc atatcaccag ctcaccgtct ttcattgcca tacgaaattc
     1081 cggatgagca ttcatcaggc gggcaagaat gtgaataaag gccggataaa acttgtgctt
     1141 atttttcttt acggtcttta aaaaggccgt aatatccagc tgaacggtct ggttataggt
     1201 acattgagca actgactgaa atgcctcaaa atgttcttta cgatgccatt gggatatatc
     1261 aacggtggta tatccagtga tttttttctc cattttagct tccttagctc ctgaaaatct
     1321 cgataactca aaaaatacgc ccggtagtga tcttatttca ttatggtgaa agttggaacc
     1381 tcttacgtgc ccgatcaact cgagtgccac ctgacgtcta agaaaccatt attatcatga
     1441 cattaaccta taaaaatagg cgtatcacga ggcagaattt cagataaaaa aaatccttag
     1501 ctttcgctaa ggatgatttc tggaattcgg tctcggcttc tctagctaga gtcgatcgac
     1561 aagctcgagt ttctccataa taatgtgtga gtagttccca gataagggaa ttagggttcc
     1621 tatagggttt cgctcatgtg ttgagcatat aagaaaccct tagtatgtat ttgtatttgt
     1681 aaaatacttc tatcaataaa atttctaatt cctaaaacca aaatccagta ctaaaatcca
     1741 gatcgcttga gaccctgcag tccggcaaaa aagggcaagg tgtcaccacc ctgccctttt
     1801 tctttaaaac cgaaaagatt acttcgcgtt atgcaggctt cctcgctcac tgactcgctg
     1861 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     1921 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     1981 aggaaccgta aaaaggccgc gttgctggcg tttttccaca ggctccgccc ccctgacgag
     2041 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     2101 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     2161 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     2221 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgt
//
