LOCUS       sequence-275154-        2324 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3TERM after CDS/ CDS12-CTAG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Sauret-Gueto S, Frangedakis E, Silvestri L, Rebmann M, Tomaselli M, 
            Markel K, Delmans M, West A, Patron NJ, Haseloff J
  TITLE     Systematic tools for reprogramming plant gene expression in a simple
            model, Marchantia polymorpha.
  JOURNAL   ACS Synth Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511.
  PUBMED    32163700
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511."
COMMENT     Sequence Label: 3TERM_Nos
FEATURES             Location/Qualifiers
     source          1..2324
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     410..429
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     663..680
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(704..761)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     terminator      complement(781..1033)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      1061..1104
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(1108..1126)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             1288..1947
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1354..1373)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1968..2062
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      join(2245..2324,1..509)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
       61 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
      121 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
      181 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
      241 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
      301 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
      361 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
      421 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
      481 gctcgtcagg ggggcggagc ctgtggaaaa acgccagcaa cgcggccttt ttacggttcc
      541 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
      601 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
      661 gcagcgagtc agtgagcgag gaagcctgca taacgcgaag taatcttttc ggttttaaag
      721 aaaaagggca gggtggtgac accttgccct tttttgccgg actgcagggt ctcaagcgtc
      781 gatctagtaa catagatgac accgcgcgcg ataatttatc ctagtttgcg cgctatattt
      841 tgttttctat cgcgtattaa atgtataatt gcgggactct aatcataaaa acccatctca
      901 taaataacgt catgcattac atgttaatta ttacatgctt aacgtaattc aacagaaatt
      961 atatgataat catcgcaaga ccggcaacag gattcaatct taagaaactt tattgccaaa
     1021 tgtttgaacg atctgcttga caagccgaga ccgaattcca gaaatcatcc ttagcgaaag
     1081 ctaaggattt tttttatctg aaattctgcc tcgtgatacg cctattttta taggttaatg
     1141 tcatgataat aatggtttct tagacgtcag gtggcactcg agttgatcgg gcacgtaaga
     1201 ggttccaact ttcaccataa tgaaataaga tcactaccgg gcgtattttt tgagttatcg
     1261 agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt
     1321 gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt
     1381 acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat
     1441 aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg
     1501 gaatttcgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt
     1561 tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga ataccacgac
     1621 gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg
     1681 gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa tccctgggtg
     1741 agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc
     1801 accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt
     1861 catcatgccg tttgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac
     1921 tgcgatgagt ggcagggcgg ggcgtaattt gatatcgagc tcgcttggac tcctgttgat
     1981 agatccagta atgacctcag aactccatct ggatttgttc agaacgctcg gttgccgccg
     2041 ggcgtttttt attggtgaga atccaagcct cgagctgtca gaccaagttt actcatatat
     2101 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     2161 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     2221 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     2281 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccgg
//
