LOCUS       sequence-275155-        2534 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3TERM after CDS/ CDS12-CTAG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Sauret-Gueto S, Frangedakis E, Silvestri L, Rebmann M, Tomaselli M, 
            Markel K, Delmans M, West A, Patron NJ, Haseloff J
  TITLE     Systematic tools for reprogramming plant gene expression in a simple
            model, Marchantia polymorpha.
  JOURNAL   ACS Synth Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511.
  PUBMED    32163700
REFERENCE   2  (bases 1 to 2534)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511."
COMMENT     Sequence Label: 3TERM_Nos-35S
FEATURES             Location/Qualifiers
     source          1..2534
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     55..73
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(77..120)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      140..392
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     polyA_signal    439..613
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     terminator      630..687
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(711..728)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(882..1470)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(962..981)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1653..1747)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1768..2427)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2342..2361
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 cgagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
       61 cgtatcacga ggcagaattt cagataaaaa aaatccttag ctttcgctaa ggatgatttc
      121 tggaattcgg tctcagcttg atcgttcaaa catttggcaa taaagtttct taagattgaa
      181 tcctgttgcc ggtcttgcga tgattatcat ataatttctg ttgaattacg ttaagcatgt
      241 aataattaac atgtaatgca tgacgttatt tatgagatgg gtttttatga ttagagtccc
      301 gcaattatac atttaatacg cgatagaaaa caaaatatag cgcgcaaact aggataaatt
      361 atcgcgcgcg gtgtcatcta tgttactaga tcgatccgta tcgatagcct ctagctagag
      421 tcgatcgaca agctcgagtt tctccataat aatgtgtgag tagttcccag ataagggaat
      481 tagggttcct atagggtttc gctcatgtgt tgagcatata agaaaccctt agtatgtatt
      541 tgtatttgta aaatacttct atcaataaaa tttctaattc ctaaaaccaa aatccagtac
      601 taaaatccag atcgctcgag accctgcagt ccggcaaaaa agggcaaggt gtcaccaccc
      661 tgcccttttt ctttaaaacc gaaaagatta cttcgcgtta tgcaggcttc ctcgctcact
      721 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
      781 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
      841 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccacag gctccgcccc
      901 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
      961 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     1021 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     1081 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     1141 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     1201 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     1261 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     1321 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     1381 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     1441 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     1501 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     1561 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     1621 gagtaaactt ggtctgacag ctcgaggctt ggattctcac caataaaaaa cgcccggcgg
     1681 caaccgagcg ttctgaacaa atccagatgg agttctgagg tcattactgg atctatcaac
     1741 aggagtccaa gcgagctcga tatcaaatta cgccccgccc tgccactcat cgcagtactg
     1801 ttgtaattca ttaagcattc tgccgacatg gaagccatca caaacggcat gatgaacctg
     1861 aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac
     1921 gggggcgaag aagttgtcca tattggccac gtttaaatca aaactggtga aactcaccca
     1981 gggattggct gagacgaaaa acatattctc aataaaccct ttagggaaat aggccaggtt
     2041 ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg
     2101 gtattcactc cagagcgatg aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg
     2161 gtgaacacta tcccatatca ccagctcacc gtctttcatt gccatacgaa attccggatg
     2221 agcattcatc aggcgggcaa gaatgtgaat aaaggccgga taaaacttgt gcttattttt
     2281 ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg gtctggttat aggtacattg
     2341 agcaactgac tgaaatgcct caaaatgttc tttacgatgc cattgggata tatcaacggt
     2401 ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa atctcgataa
     2461 ctcaaaaaat acgcccggta gtgatcttat ttcattatgg tgaaagttgg aacctcttac
     2521 gtgcccgatc aact
//
