LOCUS       sequence-275156-        5019 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PROM for cloning with 5UTR_MpNOP1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5019)
  AUTHORS   Sauret-Gueto S, Frangedakis E, Silvestri L, Rebmann M, Tomaselli M, 
            Markel K, Delmans M, West A, Patron NJ, Haseloff J
  TITLE     Systematic tools for reprogramming plant gene expression in a simple
            model, Marchantia polymorpha.
  JOURNAL   ACS Synth Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511.
  PUBMED    32163700
REFERENCE   2  (bases 1 to 5019)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511."
COMMENT     Sequence Label: PROM_MpNOP1
FEATURES             Location/Qualifiers
     source          1..5019
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      2198..2241
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(2245..2263)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             2425..3084
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2491..2510)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      3105..3199
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      3382..3970
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3871..3890
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4124..4141
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4165..4222)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
ORIGIN
        1 aattaatact gcaccgccgg ccgcaactgg gggtgcattc ttgacctaaa agttaacact
       61 ttccgtgtag aaaatacgct cgtcactcgt cttactcttc ataaatacat cttgggaatg
      121 actcggcaag aaagttatac aatacctgta tagcccccgc tgaacttttc ttggacatca
      181 aatatagata tttatcaatc ctttaaaggg agatatattt ctagcctatc atgaagtgag
      241 taagtagatt cgaaaccaaa catggaacaa acagtcgttc attgcatttt tataagctta
      301 aaaatccaac actgtcaggt ccttattctt cttctagata attatatttg taatcaagat
      361 ttcacttaac atcgatctac ttgataattg cttcattctt ttttcatacg agtttttttc
      421 atgatactca acttaataag tatatcgatc ctaaatgaaa tataatagtt aaaaacaaat
      481 ttattttatc agtatcagta taaccctaaa ttctggtttc ggaacatttg cagtgttaaa
      541 ttattcacat gtccttcaat gtatatcata gtgactaaga tatacatctg catttatatc
      601 taccaacgta taagaaataa tttactaact taaattttga attatatttt acgagaaaat
      661 gaatcaaata agacacaata atattaccgc gatgaataca ttcgcatctt ttttgcagaa
      721 agttaatcaa ggacatgaaa gtttaaaaaa atgatcaatg aatttaatcc agaagtaaaa
      781 aacttattac agtggaaaat aagagcattg gtatttgatc ttccatgttt gtaactaaaa
      841 tatcagactg ggttataaac cagagtgcaa ctgatacgtc attgctcaaa gagtgagaac
      901 aatttaaatt ttaatattta tgtaaataag tactagattg ataggataat gatgatatgt
      961 aaatgattca tttatatcaa tttagtagaa aatttacatc aaaatttgtt tccacttctc
     1021 aaaagaaaaa gttcataata cagatatact tcaagtttgt tgcagataaa tataacgtga
     1081 tcatggagaa aatcatgatg tagatcatcc tatatttgcc atcatccatc aacatcatta
     1141 tattaatcta ccttacccct tttatttgta tacaaattct caagttcaga aaacttataa
     1201 ttttattttc atatttcatt gaaggttgag tgaaggtatg aatcataatg tggggaattc
     1261 ctctcattga aacagaactt gtacacgata tgaacgtatt gtagacccca aacctgggaa
     1321 ttatccataa ccttaaccga acaaagaaac agaagccgaa agctattctt cgtctttctt
     1381 cttttgaaca taatatttcg cgctgaaatc ttgaaaatat atgaaaacta agctggtttc
     1441 agatatggag tgactaagat gcccaattat attagtaatt aagatcagag tgagaaatta
     1501 aaaaatgcat ttgatggcta attttgtcat ttctgtccac attgaatagt gctacatccg
     1561 tcacatgaga tattgcaaaa aataaatgtt actaacaatg aattatgcag ctgcatcatt
     1621 ctattcactt taaattgctc aatgtttacg atgccatgtt gatgtttaaa caatatcatg
     1681 cggaaacgtc tctaatgaaa tgctctcgtc tgaaaatgtg cagttcctta taaggacatc
     1741 cagttgagct cgaactatgt gctgggaacg acatttcgct gcaatggagt cagcccgagg
     1801 acagtcgcga cgtcgctagg gagaagtatg acgtgttgga tcaggaagat gttgtgactg
     1861 tgagacgatc gcatttcaaa gctcgtgtca tcaacaatca acagaactct gtgcaatcag
     1921 cgacgtttct ggaacccagt agagcgtcta ccgccatcta tgcctggttg gatcggatga
     1981 gtgagccgta atcttgcgct cgaacaatca tgcgtgctct ccatccgtcg attccttcac
     2041 acgccaaaga aatcctctac gatatgcttc cacgacgtct cttcgagtct gagattccgc
     2101 acagattcct ctctcggctt ccagttcgtc cctgcgaatg tccaatgaca accctgtgat
     2161 ttagcgacct tccgagaact cccgagaccg aattccagaa atcatcctta gcgaaagcta
     2221 aggatttttt ttatctgaaa ttctgcctcg tgatacgcct atttttatag gttaatgtca
     2281 tgataataat ggtttcttag acgtcaggtg gcactcgagt tgatcgggca cgtaagaggt
     2341 tccaactttc accataatga aataagatca ctaccgggcg tattttttga gttatcgaga
     2401 ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac caccgttgat
     2461 atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc tcaatgtacc
     2521 tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa gaaaaataag
     2581 cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc tcatccggaa
     2641 tttcgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca cccttgttac
     2701 accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata ccacgacgat
     2761 ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga aaacctggcc
     2821 tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc ctgggtgagt
     2881 ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc cgttttcacc
     2941 atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat tcaggttcat
     3001 catgccgttt gtgatggctt ccatgtcggc agaatgctta atgaattaca acagtactgc
     3061 gatgagtggc agggcggggc gtaatttgat atcgagctcg cttggactcc tgttgataga
     3121 tccagtaatg acctcagaac tccatctgga tttgttcaga acgctcggtt gccgccgggc
     3181 gttttttatt ggtgagaatc caagcctcga gctgtcagac caagtttact catatatact
     3241 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     3301 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     3361 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     3421 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     3481 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     3541 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     3601 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     3661 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     3721 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     3781 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     3841 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     3901 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     3961 cctgtggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     4021 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     4081 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     4141 ggaagcctgc ataacgcgaa gtaatctttt cggttttaaa gaaaaagggc agggtggtga
     4201 caccttgccc ttttttgccg gactgcaggg tctcaagtat cgccatcgag ccaagctctc
     4261 aatgacttat tcaattgctt tgacacgcgc atcgctcgcg gatgctcctg cagcttccgc
     4321 tgccccgcct ggcccgcact cctgcgcttc gaggccttta ttattttaat gcggactttt
     4381 gtgcctcacg ccattttcag tatttcgcct ttcctctcgt tccctcgctc gctggccgcg
     4441 ccccctttcc gtgccctgcc cttcgactgc tgcgtcgccg tcgtagattc ttccgccccc
     4501 ggcctgccgc ttccttccgt ccttccttcc ttccatgttc cccgtcgtca ggtggaagcc
     4561 gtgaccccaa cccaagcccc aaggcgggcg tccgtcatca ttgtccaatc caatccggac
     4621 gcctgctgtc ggatcctgcg tgcatgccgt tcctgctcga cgtcgcggcc tgcacccaat
     4681 tggactttac tacgtggacc ctgtgtcctg acgtggcatt ggatttgcac ccatttctgg
     4741 ggcagtgcgt tcggatatgt ggatggcgca ccaagtctct tcgcccgcct cgtacgcggg
     4801 ggaccgaatt ccctttgaca taatcacgtc catcgcaggt atatcctcct gtaaagtgtg
     4861 caagtatttt agctttatcg accgggcgtg catctgtaaa gagatattta catgacatga
     4921 tagaggcaag cctagcgctc gagtggggct aggatcccca ggtcatgtat tttgatcgga
     4981 tacttcttgt ataggactcc agagacaacc ggagcaccg
//
