LOCUS       sequence-275158-        3174 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3TERM after CDS/ CDS12-CTAG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Sauret-Gueto S, Frangedakis E, Silvestri L, Rebmann M, Tomaselli M, 
            Markel K, Delmans M, West A, Patron NJ, Haseloff J
  TITLE     Systematic tools for reprogramming plant gene expression in a simple
            model, Marchantia polymorpha.
  JOURNAL   ACS Synth Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511.
  PUBMED    32163700
REFERENCE   2  (bases 1 to 3174)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511."
COMMENT     Sequence Label: 3TERM_MpNOP1
FEATURES             Location/Qualifiers
     source          1..3174
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     154..171
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(195..252)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     terminator      1402..1445
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(1449..1467)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             1629..2288
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1695..1714)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      2309..2403
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     3075..3094
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
       61 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
      121 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcctgc
      181 ataacgcgaa gtaatctttt cggttttaaa gaaaaagggc agggtggtga caccttgccc
      241 ttttttgccg gactgcaggg tctcaagcga cgctcacttg ttgacgaagc ggatccgagt
      301 ggatagagcc cttgctcctc cactggagcg accagccccg tccgtaagct ggctcgagat
      361 cactgttctc gtaagcatgt tctctgcgtg agacggtcca cagatacaac gtcgacgtgc
      421 aattttgcaa gcgacaacgt gcgccaaaga gttctgcctt aaggccaaac aaacacacac
      481 acacatgaca cacacggtgc acaaattctg aacagaacct gcacagaaag cctgggctgg
      541 tagtctgaat attcaactga acaggctcag agccgacggt tacaaatcgc aaagctttcc
      601 aagcttcgaa atggaggatt ttgactgaca tacgcttaaa gaatatgctt catttaaaaa
      661 gagggagatg gaggggagag caccttcgtt gacatggagc tgacatataa tcaagtctta
      721 catctaaaaa aacctgtaag cttatatggg taggggacgt cttgagagtc ggagatcaga
      781 tcatggtcaa tcagatggac gctactggct cgcaaattcg acgactccag cccggctacc
      841 ggcagaaagg tcagccccac ttgaccccat ctcgatgtga ggagcttttt gtataatgct
      901 cggtgtaaat acatggggtt ggggttgggc ctagtctcac tgctaaatct tttgatgttt
      961 ctatcaccta tggagttaag ttttaaattc gtaatcacag ataatctaac aaaccctaac
     1021 cgatgaaccc ggcgatcgac caaactcgcc tgctgctgct ccgagggcgc atccgtcgcc
     1081 aagaccgact ctgggctgtt caggttcaac tcgtattttg acatgttaca ccgagaagtc
     1141 gtggagccct cgtccaattc atcaacatca ctttcaccag gcttcgtcgg tatgaaagga
     1201 atcaatccaa gtacaacgca tcagaacaat tccggcttgg caggcaggca ggcttgcggc
     1261 cggtggcacg cgtctgccac cgctgttata aaaggtgcaa gttggcgcac cttgctgctt
     1321 aaacttcaga ccagcctcgg ctggatttga aagtggaagg accacaactc tgccctgcac
     1381 tcaagccgag accgaattcc agaaatcatc cttagcgaaa gctaaggatt ttttttatct
     1441 gaaattctgc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     1501 ttagacgtca ggtggcactc gagttgatcg ggcacgtaag aggttccaac tttcaccata
     1561 atgaaataag atcactaccg ggcgtatttt ttgagttatc gagattttca ggagctaagg
     1621 aagctaaaat ggagaaaaaa atcactggat ataccaccgt tgatatatcc caatggcatc
     1681 gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg tacctataac cagaccgttc
     1741 agctggatat tacggccttt ttaaagaccg taaagaaaaa taagcacaag ttttatccgg
     1801 cctttattca cattcttgcc cgcctgatga atgctcatcc ggaatttcgt atggcaatga
     1861 aagacggtga gctggtgata tgggatagtg ttcacccttg ttacaccgtt ttccatgagc
     1921 aaactgaaac gttttcatcg ctctggagtg aataccacga cgatttccgg cagtttctac
     1981 acatatattc gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt
     2041 ttattgagaa tatgtttttc gtctcagcca atccctgggt gagtttcacc agttttgatt
     2101 taaacgtggc caatatggac aacttcttcg cccccgtttt caccatgggc aaatattata
     2161 cgcaaggcga caaggtgctg atgccgctgg cgattcaggt tcatcatgcc gtttgtgatg
     2221 gcttccatgt cggcagaatg cttaatgaat tacaacagta ctgcgatgag tggcagggcg
     2281 gggcgtaatt tgatatcgag ctcgcttgga ctcctgttga tagatccagt aatgacctca
     2341 gaactccatc tggatttgtt cagaacgctc ggttgccgcc gggcgttttt tattggtgag
     2401 aatccaagcc tcgagctgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     2461 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     2521 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     2581 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     2641 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     2701 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     2761 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     2821 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     2881 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     2941 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     3001 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     3061 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     3121 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctgtg gaaa
//
