LOCUS       sequence-27517-e         826 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Zeiser E, Frokjaer-Jensen C, Jorgensen E, Ahringer J
  TITLE     MosSCI and gateway compatible plasmid toolkit for constitutive and 
            inducible expression of transgenes in the C. elegans germline.
  JOURNAL   PLoS One. 2011;6(5):e20082. Epub 2011 May 26.
  PUBMED    21637852
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(5)"; pages: "e20082. Epub 2011 May 26"
COMMENT     Sequence Label: pJA254
FEATURES             Location/Qualifiers
     source          1..826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(509..604)
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(644..661)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(644..660)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(653..675)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 ggaagtctct cctttctcat tctctgtctg aaacattcaa ttgattatcg ataaaattaa
       61 ttaagagaga atacaatgag aaaacggaaa atcgagcagg attttgggga aagtcgggga
      121 atttttgcta tttaagcttt ctttaacaaa gaaactgcat ttttccatga gattaccatg
      181 aaatttgcgt taaatgggtg aatttataac atttacggct agaaattaat tttaaaaaag
      241 agaataaacg aaaaattatt cgagaaaacg tctttgaagc agaaatgaaa tgaaaaaaca
      301 accgccagcg ctgacaaaaa tgatcaaaca aacggagtac ggtatacgcg gtaagaccca
      361 aatgaatttt aattcggtgc gcctttaaaa aatgtatttt tttaatttct ttatccgtgg
      421 gcccaaatgt atttttttct tgtggtttga tgtaaaaatt gggttatatt atttgtttta
      481 tggtttaatt ttttaaaaac tgatatttca acttttctat acaaagttgg cattataaaa
      541 aagcattgct tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt
      601 atttggagct ccatggtagc gttaactcgg gcccgagctt aagactggcc gtcgttttac
      661 aacgtcgtga ctgggaaaac atccatgcta gcgttaacgc gagagtaggg aactgccagg
      721 ctcaataaaa cgaaggctca ncggagacgg gccttcgtta ctgtgttgcg ggacctccng
      781 anagcatcgc ngacgatgac tggacccggc gggggggggn gccccc
//
