LOCUS       sequence-27518-e         583 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 583)
  AUTHORS   Zeiser E, Frokjaer-Jensen C, Jorgensen E, Ahringer J
  TITLE     MosSCI and gateway compatible plasmid toolkit for constitutive and 
            inducible expression of transgenes in the C. elegans germline.
  JOURNAL   PLoS One. 2011;6(5):e20082. Epub 2011 May 26.
  PUBMED    21637852
REFERENCE   2  (bases 1 to 583)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(5)"; pages: "e20082. Epub 2011 May 26"
COMMENT     Sequence Label: pJA254
FEATURES             Location/Qualifiers
     source          1..583
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(68..191)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(251..271)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 tcgcggccgc cctgcagctc tagagctcga attctacagg tcactaatac catctaagta
       61 gttggttcat agtgactgca tatgttgtgt tttacagtat tatgtagtct gttttttatg
      121 caaaatctaa tttaatatat tgatatttat atcattttac gtttctcgtt caactttttt
      181 gtacaaactt gaagctccac ctccacctcc tttgtatagt tcgtccatgc catgtgtaat
      241 cccagcagct gttacaaact caagaaggac catgtggtct ctcttttcgt tgggatcttt
      301 cgaaagggca gattgtgtgg acaggtaatg gttgtctggt aaaaggacag ggccatcgcc
      361 aattggagta ttttgttgat aatggtctgc tagttgaacg cttccatctt caatgttgtg
      421 tctaattttg aagttctgaa aatttaaatc agattagtta gttagtaaaa tcatgtttaa
      481 acttacaact ttgattccat tcttttgttt gtctgccatg atgtatacat tgtgtgagtt
      541 atagttgtat tccaatttgt gtccaagaat gtttcccatc ttc
//
