LOCUS       sequence-275188-        5224 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PapFAB45 - apFAB682 - EsaI - LuxR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5224)
  AUTHORS   Glasscock CJ, Biggs BW, Lazar JT, Arnold JH, Burdette LA, Valdes A, 
            Kang MK, Tullman-Ercek D, Tyo KEJ, Lucks JB
  TITLE     Dynamic Control of Gene Expression with Riboregulated Switchable 
            Feedback Promoters.
  JOURNAL   ACS Synth Biol. 2021 May 21;10(5):1199-1213. doi: 
            10.1021/acssynbio.1c00015. Epub 2021 Apr 9.
  PUBMED    33834762
REFERENCE   2  (bases 1 to 5224)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00015"; journalName: "ACS Synth Biol"; date: 
            "2021-05-21- 21"; volume: "10"; issue: "5"; pages: "1199-1213"
COMMENT     Sequence Label: pJBL6691
FEATURES             Location/Qualifiers
     source          1..5224
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      39..110
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      126..153
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(354..1304)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      1352..1574
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(2066..2160)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             2354..3145
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRTDQLEEFVHYVKGEITKVVGK"
     promoter        complement(3338..3442)
                     /gene="bla"
                     /label=AmpR promoter
     RBS             4454..4465
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 tctagaggat ccaaactcga gtaaggatct ccaggcatca aataaaacga aaggctcagt
       61 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc
      121 acactggctc accttcgggt gggcctttct gcgtttatac ctaggggatc cttactcgag
      181 tctagactgc agcaccaaaa actcgtaaaa gctctgatgt atctatcttt tttacaccgt
      241 tttcatctgt gcatatggac agttttccct ttgatatgta acggtgaaca gttgttctac
      301 ttttgtttgt tagtcttgat gcttcactga tagatacaag agccataaga acctcagatc
      361 cttccgtatt tagccagtat gttctctagt gtggttcgtt gtttttgcgt gagccatgag
      421 aacgaaccat tgagatcata cttactttgc atgtcactca aaaattttgc ctcaaaactg
      481 gtgagctgaa tttttgcagt taaagcatcg tgtagtgttt ttcttagtcc gttatgtagg
      541 taggaatctg atgtaatggt tgttggtatt ttgtcaccat tcatttttat ctggttgttc
      601 tcaagttcgg ttacgagatc catttgtcta tctagttcaa cttggaaaat caacgtatca
      661 gtcgggcggc ctcgcttatc aaccaccaat ttcatattgc tgtaagtgtt taaatcttta
      721 cttattggtt tcaaaaccca ttggttaagc cttttaaact catggtagtt attttcaagc
      781 attaacatga acttaaattc atcaaggcta atctctatat ttgccttgtg agttttcttt
      841 tgtgttagtt cttttaataa ccactcataa atcctcatag agtatttgtt ttcaaaagac
      901 ttaacatgtt ccagattata ttttatgaat ttttttaact ggaaaagata aggcaatatc
      961 tcttcactaa aaactaattc taatttttcg cttgagaact tggcatagtt tgtccactgg
     1021 aaaatctcaa agcctttaac caaaggattc ctgatttcca cagttctcgt catcagctct
     1081 ctggttgctt tagctaatac accataagca ttttccctac tgatgttcat catctgagcg
     1141 tattggttat aagtgaacga taccgtccgt tctttccttg tagggttttc aatcgtgggg
     1201 ttgagtagtg ccacacagca taaaattagc ttggtttcat gctccgttaa gtcatagcga
     1261 ctaatcgcta gttcatttgc tttgaaaaca actaattcag acatacatct caattggtct
     1321 aggtgatttt aatcactata ccaattgaga tgggctagtc aatgataatt acatgtcctt
     1381 ttcctttgag ttgtgggtat ctgtaaattc tgctagacct ttgctggaaa acttgtaaat
     1441 tctgctagac cctctgtaaa ttccgctaga cctttgtgtg ttttttttgt ttatattcaa
     1501 gtggttataa tttatagaat aaagaaagaa taaaaaaaga taaaaagaat agatcccagc
     1561 cctgtgtata actcactact ttagtcagtt ccgcagtatt acaaaaggat gtcgcaaacg
     1621 ctgtttgctc ctctacaaaa cagaccttaa aaccctaaag gcttaagtag caccctcgca
     1681 agctcgggca aatcgctgaa tattcctttt gtctccgacc atcaggcacc tgagtcgctg
     1741 tctttttcgt gacattcagt tcgctgcgct cacggctctg gcagtgaatg ggggtaaatg
     1801 gcactacagg cgccttttat ggattcatgc aaggaaacta cccataatac aagaaaagcc
     1861 cgtcacgggc ttctcagggc gttttatggc gggtctgcta tgtggtgcta tctgactttt
     1921 tgctgttcag cagttcctgc cctctgattt tccagtctga ccacttcgga ttatcccgtg
     1981 acaggtcatt cagactggct aatgcaccca gtaaggcagc ggtatcatca acaggcttac
     2041 ccgtcttact gtccctagtg cttggattct caccaataaa aaacgcccgg cggcaaccga
     2101 gcgttctgaa caaatccaga tggagttctg aggtcattac tggatctatc aacaggagtc
     2161 caagcgagct cgtaaacttg gtaacggcgc agtggcggtt ttcatggctt gttatgactg
     2221 tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg
     2281 tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac
     2341 aaagttaaac atcatgaggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt
     2401 agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc
     2461 cgcagtggat ggcggcctga agccacacag tgatattgat ttgctggtta cggtgaccgt
     2521 aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc
     2581 ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat
     2641 cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga
     2701 cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac
     2761 aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc
     2821 ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc
     2881 gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag
     2941 cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct
     3001 gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga
     3061 agatcgcttg gcctcgcgca cagatcagtt ggaagaattt gtccactacg tgaaaggcga
     3121 gatcaccaag gtagtcggca aataaaaaac gttcttcggg gcgaaaactc tcaaggatct
     3181 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     3241 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     3301 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     3361 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     3421 ataaacaaat aggggttccg cgcacatttc ccatggtgcc acctgacgtc taagaaacca
     3481 ttattatcat gacattaacc tataaaaata gtcgtatcac gaggcagaat ttcaggagcg
     3541 gtatcagctc actcaaaggc gtatcacgag gcagaatttc aggagcggta tcagctcact
     3601 caaaggcggt aatctcgagg ttacattgtc gatctgttca tggtgaacag ctttaaatct
     3661 ttatgcttcc ggctgaattc taaagatctt tgcctcttaa tcatcggctc gtataatgtg
     3721 tggagggccc aagttcactt aaaaaggaga tcaacaatga aagcaatttt cgtactgaaa
     3781 catcttaatc atgcgaggga tggtttctaa tgatgcttga actgtttgac gtcagttacg
     3841 aagaactgca aaccacccgt tcagaagaac tttataaact tcgcaagaaa acatttagcg
     3901 atcgtctggg atgggaagtc atttgcagtc agggaatgga gtccgatgaa tttgatgggc
     3961 ccggtacacg ttatattctg ggaatctgcg aaggacaatt agtgtgcagc gtacgtttta
     4021 ccagcctcga tcgtcccaac atgatcacgc acacttttca gcactgcttc agtgatgtca
     4081 ccctgcccgc ctatggtacc gaatccagcc gtttttttgt cgacaaagcc cgcgcacgtg
     4141 cgctgttagg tgagcactac cctatcagcc aggtcctgtt tttagcgatg gtgaactggg
     4201 cgcaaaataa tgcctacggc aatatctata cgattgtcag ccgcgcgatg ttgaaaattc
     4261 tcactcgctc tggctggcaa atcaaagtca ttaaagaggc tttcctgacc gaaaaggaac
     4321 gtatctattt gctgacgctg ccagcaggtc aggatgacaa gcagcaactc ggtggtgatg
     4381 tggtgtcacg tacgggctgt ccgcccgtcg cagtcactac ctggccgctg acgctgccgg
     4441 tctgatacta gagaaagagg agaaatacta gatgaaaaac ataaatgccg acgacacata
     4501 cagaataatt aataaaatta aagcttgtag aagcaataat gatattaatc aatgcttatc
     4561 tgatatgact aaaatggtac attgtgaata ttatttactc gcgatcattt atcctcattc
     4621 tatggttaaa tctgatattt caatcctaga taattaccct aaaaaatgga ggcaatatta
     4681 tgatgacgct aatttaataa aatatgatcc tatagtagat tattctaact ccaatcattc
     4741 accaattaat tggaatatat ttgaaaacaa tgctgtaaat aaaaaatctc caaatgtaat
     4801 taaagaagcg aaaacatcag gtcttatcac tgggtttagt ttccctattc atacggctaa
     4861 caatggcttc ggaatgctta gttttgcaca ttcagaaaaa gacaactata tagatagttt
     4921 atttttacat gcgtgtatga acataccatt aattgttcct tctctagttg ataattatcg
     4981 aaaaataaat atagcaaata ataaatcaaa caacgattta accaaaagag aaaaagaatg
     5041 tttagcgtgg gcatgcgaag gaaaaagctc ttgggatatt tcaaaaatat taggttgcag
     5101 tgagcgtact gtcactttcc atttaaccaa tgcgcaaatg aaactcaata caacaaaccg
     5161 ctgccaaagt atttctaaag caattttaac aggagcaatt gattgcccat actttaaaaa
     5221 ttaa
//
