LOCUS       sequence-275191-        3551 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PcarA - TARGET8 - mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3551)
  AUTHORS   Glasscock CJ, Biggs BW, Lazar JT, Arnold JH, Burdette LA, Valdes A, 
            Kang MK, Tullman-Ercek D, Tyo KEJ, Lucks JB
  TITLE     Dynamic Control of Gene Expression with Riboregulated Switchable 
            Feedback Promoters.
  JOURNAL   ACS Synth Biol. 2021 May 21;10(5):1199-1213. doi: 
            10.1021/acssynbio.1c00015. Epub 2021 Apr 9.
  PUBMED    33834762
REFERENCE   2  (bases 1 to 3551)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00015"; journalName: "ACS Synth Biol"; date: 
            "2021-05-21- 21"; volume: "10"; issue: "5"; pages: "1199-1213"
COMMENT     Sequence Label: pJBL6662
FEATURES             Location/Qualifiers
     source          1..3551
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(171..962)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(963..1054)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1102..1840)
                     /direction=LEFT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     CDS             2721..3398
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      3431..3502
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3518..3545
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 ctgaaacctc aggcatttga gaagcacacg gtcacactgc ttccggtagt caataaaccg
       61 gtaaaccagc aatagacata agcggctatt taacgaccct gccctgaacc gacgaccggg
      121 tcatcgtggc cggatcttgc ggcccctcgg cttgaacgaa ttgttagaca ttatttgccg
      181 actaccttgg tgatctcgcc tttcacgtag tggacaaatt cttccaactg atctgcgcgc
      241 gaggccaagc gatcttcttc ttgtccaaga taagcctgtc tagcttcaag tatgacgggc
      301 tgatactggg ccggcaggcg ctccattgcc cagtcggcag cgacatcctt cggcgcgatt
      361 ttgccggtta ctgcgctgta ccaaatgcgg gacaacgtaa gcactacatt tcgctcatcg
      421 ccagcccagt cgggcggcga gttccatagc gttaaggttt catttagcgc ctcaaataga
      481 tcctgttcag gaaccggatc aaagagttcc tccgccgctg gacctaccaa ggcaacgcta
      541 tgttctcttg cttttgtcag caagatagcc agatcaatgt cgatcgtggc tggctcgaag
      601 atacctgcaa gaatgtcatt gcgctgccat tctccaaatt gcagttcgcg cttagctgga
      661 taacgccacg gaatgatgtc gtcgtgcaca acaatggtga cttctacagc gcggagaatc
      721 tcgctctctc caggggaagc cgaagtttcc aaaaggtcgt tgatcaaagc tcgccgcgtt
      781 gtttcatcaa gccttacggt caccgtaacc agcaaatcaa tatcactgtg tggcttcagg
      841 ccgccatcca ctgcggagcc gtacaaatgt acggccagca acgtcggttc gagatggcgc
      901 tcgatgacgc caactacctc tgatagttga gtcgatactt cggcgatcac cgcttccctc
      961 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     1021 tacatatttg aatgtattta gaaaaataaa caaatagcta gctcactcgg tcgctacgct
     1081 ccgggcgtga gactgcggcg ggcgctgcgg acacatacaa agttacccac agattccgtg
     1141 gataagcagg ggactaacat gtgaggcaaa acagcagggc cgcgccggtg gcgtttttcc
     1201 ataggctccg ccctcctgcc agagttcaca taaacagacg cttttccggt gcatctgtgg
     1261 gagccgttag gctcaaccat gaatctgaca gtacgggcga aacccgacag gacttaaaga
     1321 tccccaccgt ttccggcggg tcgctccctc ttgcgctctc ctgttccgac cctgccgttt
     1381 accggatacc tgttccgcct ttctccctta cgggaagtgt ggcgctttct catagctcac
     1441 acactggtat ctcggctcgg tgtaggtcgt tcgctccaag ctgggctgta agcaagaact
     1501 ccccgttcag cccgactgct gcgccttatc cggtaactgt tcacttgagt ccaacccgga
     1561 aaagcacggt aaaacgccac tggcagcagc cattggtaac tgggagttcg cagaggattt
     1621 gtttagctaa acacgcggtt gctcttgaag tgtgcgccaa agtccggcta cactggaagg
     1681 acagatttgg ttgctgtgct ctgcgaaagc cagttaccac ggttaagtca gttggggaac
     1741 tgcttaacct tcgatcaaac cacctcccca ggtggttttt tcgtttacag ggcaaaagat
     1801 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctactg aaccgctcta
     1861 gatttcagtg caatttatct cttcaaatgt agcacctgaa gtcagcccca tacgatataa
     1921 gttgtaattc tcatgttagt catgccccgc gcccacagga aggagctgac tgggttgaag
     1981 gctctcaagg gcatcggtcg agatcccggt gcctaatgag tgagctaact tacattaatt
     2041 gcgttgcgcg aattcacgag atctgcggat gggcagtatt ggcgctgaac gcctgctggc
     2101 gcgtattcgt ggcgggtctt tttgatatgc gagatgtact tgatctcaat aatttgtaac
     2161 cacaaaatat ttgttatggt gcaaaaataa cacatttaat ttattgatta taaagggctt
     2221 taatttttgg cccttttatt tttggtgtta tgtttttaaa ttgtctataa gtgccaaaaa
     2281 ttacatgttt tgtcttctgt ttttgttgtt ttaatgtaaa ttttgaccat ttggtccact
     2341 tttttctgct cgtttttatt tcatgcaatc ttcttgctgc gcaagcgttt tccagaacag
     2401 gttagatgat ctttttgtcg cttaatgcct gtaaaacatg catgagccac aaaataatat
     2461 aaaaaatccc gccattaagt tgacttttag cgcccatatc tccagaatgc cgccgtttgc
     2521 cagaaattcg tcggtaagca gatttgcatt gatttacgtc atcattgtga attaatatgc
     2581 aaataaagtg agtgaatatt ctctggaggg tgttccatcc tcaatctcta cctactctca
     2641 cttactctta tcctgcgggg aatgtataca gttcatgtat atattccccg cttttttttt
     2701 ggatctagga ggaaggatct atggcgagta gcgaagacgt tatcaaagag ttcatgcgtt
     2761 tcaaagttcg tatggaaggt tccgttaacg gtcacgagtt cgaaatcgaa ggtgaaggtg
     2821 aaggtcgtcc gtacgaaggt acccagaccg ctaaactgaa agttaccaaa ggtggtccgc
     2881 tgccgttcgc ttgggacatc ctgtccccgc agttccagta cggttccaaa gcttacgtta
     2941 aacacccggc tgacatcccg gactacctga aactgtcctt cccggaaggt ttcaaatggg
     3001 aacgtgttat gaacttcgaa gacggtggtg ttgttaccgt tacccaggac tcctccctgc
     3061 aagacggtga gttcatctac aaagttaaac tgcgtggtac caacttcccg tccgacggtc
     3121 cggttatgca gaaaaaaacc atgggttggg aagcttccac cgaacgtatg tacccggaag
     3181 acggtgctct gaaaggtgaa atcaaaatgc gtctgaaact gaaagacggt ggtcactacg
     3241 acgctgaagt taaaaccacc tacatggcta aaaaaccggt tcagctgccg ggtgcttaca
     3301 aaaccgacat caaactggac atcacctccc acaacgaaga ctacaccatc gttgaacagt
     3361 acgaacgtgc tgaaggtcgt cactccaccg gtgcttaagg atccaaactc gagtaaggat
     3421 ctccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
     3481 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt
     3541 ctgcgtttat a
//
