LOCUS       sequence-275193-        4069 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mp U6 promoter (type III RNA polymerase promoter) to drive 
            expression of gRNA for CRISPR/Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4069)
  AUTHORS   Sauret-Gueto S, Frangedakis E, Silvestri L, Rebmann M, Tomaselli M, 
            Markel K, Delmans M, West A, Patron NJ, Haseloff J
  TITLE     Systematic tools for reprogramming plant gene expression in a simple
            model, Marchantia polymorpha.
  JOURNAL   ACS Synth Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511.
  PUBMED    32163700
REFERENCE   2  (bases 1 to 4069)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511."
COMMENT     Sequence Label: PROM5_MpU6
FEATURES             Location/Qualifiers
     source          1..4069
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1625..1668
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(1672..1690)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             1852..2511
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1918..1937)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      2532..2626
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      2809..3397
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3298..3317
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3551..3568
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3592..3649)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
ORIGIN
        1 acttaaggta cctgggcatg catagaagca caagaaagtt tcttggaata agctcttgca
       61 gagatatgag atagaacttg cgttgaggag tgtactagag tgttcggaat ttgttcccat
      121 acatatatgt actgtactat agtttgacaa acatggagag tgaatagacg aaggtcctct
      181 tatactaacc gccactatat tgggatgcaa aaattgatca cgatggggac ttgtacatag
      241 agaattaatt tgtttgtttt ttagtttagg atgtttaata atctcactgt ctaatgtcag
      301 gtggttgtgt ttttactttg tagtttgcgt gtgtgtgtgt gtctggtttt cgtttctatt
      361 tgtaaatcgt cagacaatta aagatattgg aatagaaagt tgtgagtttt ttgctagttg
      421 aagggtttct atagttctcg tttgaactgt gagagatcac gaagtttatg attttgctta
      481 tagagtgata cgttatggac tgcattttat ctgtatattt gacaccagta agtacagaat
      541 gctctataaa aagaaattaa gttttcagag taaaatgaaa taagattgca catattcata
      601 ttatatttac atcatatgtg atgatttttt gaaaattttc ttcgatattg tggattgttg
      661 acatatttga aaataaattt ttaactctta catataaatc ttccataggt tttttttgac
      721 aatccacaat atactttctc aaagaaaaac ataaatcatg tttttttcca taaaaaataa
      781 ataaatttgc aagatgtatg tgtcaaatag ttgattggat taaaataaaa gcccattcac
      841 cttgaaacta tgttaaattt ttgtgaaaat caagataaac cgacttttta tagtcagatt
      901 gttaaattct aattgagact tgcatgtgtt atttatctta attattcatg aaaaatctga
      961 ttatattttt tattaatcag cacataagtt tgtgaaattt tatgatcaat ccgattcatc
     1021 aagttgaaga tatattacaa gctaatgagt ataacacatt aactagcatt gttcaattgt
     1081 tattgatact atgtatgtag tttagaaaaa ttaaaatgag atgtagatga tgcagttttg
     1141 acacatctta aacatgtagc ctggattaat ttacaaattt ttgtagtcaa tcagtaatat
     1201 gattgtattt ttcttgaaat ttacaacaaa aatgtgcttt tgaaactatt attgttgtta
     1261 ttttatcttc aagttttttt tctaaaaaat tataaacaat gagagtatga taggaaaatt
     1321 tgtttttttc attaaaaagc attatgatag ctgagtttgc gatgttttga gctaaaaaat
     1381 atttaaataa ataaagatat aaacatattt tgtctcgtgg gttaaaatat atatgaaaga
     1441 tcttagtata acattatgaa actgatatgt aatatatgat ctaataataa attaaaattt
     1501 gatcaaattt tttaacgagt tcaatttttt ttaaagttat tatcatgacg tattagactt
     1561 atgagaaaga aaaattgatc tttaaaaatc tcttttgaat gaatactccc gagaccgaat
     1621 tccagaaatc atccttagcg aaagctaagg atttttttta tctgaaattc tgcctcgtga
     1681 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     1741 ctcgagttga tcgggcacgt aagaggttcc aactttcacc ataatgaaat aagatcacta
     1801 ccgggcgtat tttttgagtt atcgagattt tcaggagcta aggaagctaa aatggagaaa
     1861 aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga acattttgag
     1921 gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga tattacggcc
     1981 tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat tcacattctt
     2041 gcccgcctga tgaatgctca tccggaattt cgtatggcaa tgaaagacgg tgagctggtg
     2101 atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga aacgttttca
     2161 tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata ttcgcaagat
     2221 gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga gaatatgttt
     2281 ttcgtctcag ccaatccctg ggtgagtttc accagttttg atttaaacgt ggccaatatg
     2341 gacaacttct tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg
     2401 ctgatgccgc tggcgattca ggttcatcat gccgtttgtg atggcttcca tgtcggcaga
     2461 atgcttaatg aattacaaca gtactgcgat gagtggcagg gcggggcgta atttgatatc
     2521 gagctcgctt ggactcctgt tgatagatcc agtaatgacc tcagaactcc atctggattt
     2581 gttcagaacg ctcggttgcc gccgggcgtt ttttattggt gagaatccaa gcctcgagct
     2641 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     2701 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     2761 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     2821 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     2881 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     2941 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     3001 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     3061 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     3121 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     3181 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     3241 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     3301 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     3361 tttgtgatgc tcgtcagggg ggcggagcct gtggaaaaac gccagcaacg cggccttttt
     3421 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     3481 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     3541 gaccgagcgc agcgagtcag tgagcgagga agcctgcata acgcgaagta atcttttcgg
     3601 ttttaaagaa aaagggcagg gtggtgacac cttgcccttt tttgccggac tgcagggtct
     3661 cacgagaggc tgggtgcaac tctgctttat atccaccatt attcagttct tcactaaacg
     3721 tatggtgcaa taaatgaggc cagcgccacg tgtctcaact gtggtcattc ttcgatccac
     3781 tgtgcactgt ttgttgtctc aagaagtcag attctggacc caggaagaaa caagaaggtg
     3841 gataaatcga ccactcaaaa gttctgaaac aagtgactga ctagtgaaca gcagttcata
     3901 aattccatat ggttcgggga gacagagaaa gagagcaatc cgaactccaa ggaaactagc
     3961 cggatacatc tctcgtgacg cttcgacttt tttcttattt ttgtgcagtt gcgtcactct
     4021 gagccatcaa agggagaact gaaaaggctg tgaagtgact atgcgcgcg
//
