LOCUS       sequence-275194-        3552 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PmetN - TARGET8 - mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3552)
  AUTHORS   Glasscock CJ, Biggs BW, Lazar JT, Arnold JH, Burdette LA, Valdes A, 
            Kang MK, Tullman-Ercek D, Tyo KEJ, Lucks JB
  TITLE     Dynamic Control of Gene Expression with Riboregulated Switchable 
            Feedback Promoters.
  JOURNAL   ACS Synth Biol. 2021 May 21;10(5):1199-1213. doi: 
            10.1021/acssynbio.1c00015. Epub 2021 Apr 9.
  PUBMED    33834762
REFERENCE   2  (bases 1 to 3552)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00015"; journalName: "ACS Synth Biol"; date: 
            "2021-05-21- 21"; volume: "10"; issue: "5"; pages: "1199-1213"
COMMENT     Sequence Label: pJBL6660
FEATURES             Location/Qualifiers
     source          1..3552
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(172..963)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(964..1055)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1103..1841)
                     /direction=LEFT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     CDS             2722..3399
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      3432..3503
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3519..3546
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 ctgaaacctt caggcatttg agaagcacac ggtcacactg cttccggtag tcaataaacc
       61 ggtaaaccag caatagacat aagcggctat ttaacgaccc tgccctgaac cgacgaccgg
      121 gtcatcgtgg ccggatcttg cggcccctcg gcttgaacga attgttagac attatttgcc
      181 gactaccttg gtgatctcgc ctttcacgta gtggacaaat tcttccaact gatctgcgcg
      241 cgaggccaag cgatcttctt cttgtccaag ataagcctgt ctagcttcaa gtatgacggg
      301 ctgatactgg gccggcaggc gctccattgc ccagtcggca gcgacatcct tcggcgcgat
      361 tttgccggtt actgcgctgt accaaatgcg ggacaacgta agcactacat ttcgctcatc
      421 gccagcccag tcgggcggcg agttccatag cgttaaggtt tcatttagcg cctcaaatag
      481 atcctgttca ggaaccggat caaagagttc ctccgccgct ggacctacca aggcaacgct
      541 atgttctctt gcttttgtca gcaagatagc cagatcaatg tcgatcgtgg ctggctcgaa
      601 gatacctgca agaatgtcat tgcgctgcca ttctccaaat tgcagttcgc gcttagctgg
      661 ataacgccac ggaatgatgt cgtcgtgcac aacaatggtg acttctacag cgcggagaat
      721 ctcgctctct ccaggggaag ccgaagtttc caaaaggtcg ttgatcaaag ctcgccgcgt
      781 tgtttcatca agccttacgg tcaccgtaac cagcaaatca atatcactgt gtggcttcag
      841 gccgccatcc actgcggagc cgtacaaatg tacggccagc aacgtcggtt cgagatggcg
      901 ctcgatgacg ccaactacct ctgatagttg agtcgatact tcggcgatca ccgcttccct
      961 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     1021 atacatattt gaatgtattt agaaaaataa acaaatagct agctcactcg gtcgctacgc
     1081 tccgggcgtg agactgcggc gggcgctgcg gacacataca aagttaccca cagattccgt
     1141 ggataagcag gggactaaca tgtgaggcaa aacagcaggg ccgcgccggt ggcgtttttc
     1201 cataggctcc gccctcctgc cagagttcac ataaacagac gcttttccgg tgcatctgtg
     1261 ggagccgtta ggctcaacca tgaatctgac agtacgggcg aaacccgaca ggacttaaag
     1321 atccccaccg tttccggcgg gtcgctccct cttgcgctct cctgttccga ccctgccgtt
     1381 taccggatac ctgttccgcc tttctccctt acgggaagtg tggcgctttc tcatagctca
     1441 cacactggta tctcggctcg gtgtaggtcg ttcgctccaa gctgggctgt aagcaagaac
     1501 tccccgttca gcccgactgc tgcgccttat ccggtaactg ttcacttgag tccaacccgg
     1561 aaaagcacgg taaaacgcca ctggcagcag ccattggtaa ctgggagttc gcagaggatt
     1621 tgtttagcta aacacgcggt tgctcttgaa gtgtgcgcca aagtccggct acactggaag
     1681 gacagatttg gttgctgtgc tctgcgaaag ccagttacca cggttaagtc agttggggaa
     1741 ctgcttaacc ttcgatcaaa ccacctcccc aggtggtttt ttcgtttaca gggcaaaaga
     1801 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctact gaaccgctct
     1861 agatttcagt gcaatttatc tcttcaaatg tagcacctga agtcagcccc atacgatata
     1921 agttgtaatt ctcatgttag tcatgccccg cgcccacagg aaggagctga ctgggttgaa
     1981 ggctctcaag ggcatcggtc gagatcccgg tgcctaatga gtgagctaac ttacattaat
     2041 tgcgttgcgc gaattcacga gatctgcgga tgggcagtat tggcgctgaa cgcctgctgg
     2101 cgcgtattcg tggcgggcga aactcttcaa cactaccctg cggatgatgc gggcaataat
     2161 agataccatc cagatcgaca tctcggtccg ccagcgacca gtccatccac tcggtcagcg
     2221 tttcaaactg tgcttcggta aatttaccgc gagcaatgcc agactggttg gttactacca
     2281 ccagcgcaaa gcccattttt tttagctcgc gcatggcgtc aataacaccg tcgataaatt
     2341 caaagttgtc gatctcatgg acatagccgt gatcgacatt aatggtgcca tcacggtcaa
     2401 gaaaaattgc gggtacgctc ttcgccacct tttatagctc cttaataagg catgtgacgc
     2461 tagtatcgca tgtttcgacc tgcaagaaag tgctcttcgc ataaacctga ttgatttaga
     2521 cgtctggatg ccttaacatc catttcattg acggcgttgc ccgtttcagg cattcgagat
     2581 gccacgacta acttaatgac gataataaat aatcaccatc ctcaatctct acctactctc
     2641 acttactctt atcctgcggg gaatgtatac agttcatgta tatattcccc gctttttttt
     2701 tggatctagg aggaaggatc tatggcgagt agcgaagacg ttatcaaaga gttcatgcgt
     2761 ttcaaagttc gtatggaagg ttccgttaac ggtcacgagt tcgaaatcga aggtgaaggt
     2821 gaaggtcgtc cgtacgaagg tacccagacc gctaaactga aagttaccaa aggtggtccg
     2881 ctgccgttcg cttgggacat cctgtccccg cagttccagt acggttccaa agcttacgtt
     2941 aaacacccgg ctgacatccc ggactacctg aaactgtcct tcccggaagg tttcaaatgg
     3001 gaacgtgtta tgaacttcga agacggtggt gttgttaccg ttacccagga ctcctccctg
     3061 caagacggtg agttcatcta caaagttaaa ctgcgtggta ccaacttccc gtccgacggt
     3121 ccggttatgc agaaaaaaac catgggttgg gaagcttcca ccgaacgtat gtacccggaa
     3181 gacggtgctc tgaaaggtga aatcaaaatg cgtctgaaac tgaaagacgg tggtcactac
     3241 gacgctgaag ttaaaaccac ctacatggct aaaaaaccgg ttcagctgcc gggtgcttac
     3301 aaaaccgaca tcaaactgga catcacctcc cacaacgaag actacaccat cgttgaacag
     3361 tacgaacgtg ctgaaggtcg tcactccacc ggtgcttaag gatccaaact cgagtaagga
     3421 tctccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
     3481 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt
     3541 tctgcgttta ta
//
