LOCUS       sequence-275204-        7842 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7842)
  AUTHORS   Shen WF, Hu YL, Uttarwar L, Passegue E, Largman C
  TITLE     MicroRNA-126 regulates HOXA9 by binding to the homeobox.
  JOURNAL   Mol Cell Biol. 2008 Jul . 28(14):4609-19.
  PUBMED    18474618
REFERENCE   2  (bases 1 to 7842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2008 Jul . 28(14):4609-19."
COMMENT     Sequence Label: HOXA9-MSCV-CD24
FEATURES             Location/Qualifiers
     source          1..7842
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1981..2480
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2016..2039)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2413..2432
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      2487..2496
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     LTR             2782..3296
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    3458..3474
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3482..3488,3489..3506,3507..3512))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3527..3548
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3665..3682)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3836..4424)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3916..3935)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4595..5386,5387..5455))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5218..5237
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5456..5560)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5628..5646
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5684..5706)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5806..5825
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6019..6041
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6169..6188
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             6388..6904
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    6968..7309
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             7376..7792
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     7722..7744
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     7760..7776
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             7804..7827
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gatcccacca ccggggccct gggcaactac tatgtggact ccttcctgct gggcgccgac
       61 gctgctgatg agctgggtgc gggacgctac gctccaggga ccctgggtca acccccaagg
      121 caggcggcag ctctggccga acaccccgac ttcagtcctt gcagcttcca gtccaaggcg
      181 gcggtgtttg gtgcctcgtg gaacccagtg cacgcggcgg gcgccaatgc ggtgcctgct
      241 gcagtgtatc atcaccacca ccacccctac gtgcatcccc aggcgcccgt ggcggcggcg
      301 gcgccggacg gcaggtatat gcgctcctgg ctggaaccca cgcccggtgc gctctccttc
      361 gcgggcttac cctccagccg gccttatggc attaaacctg aaccgctctc ggccagaagg
      421 ggtgactgtc ccacgcttga cactcacact ttgtccctga ctgactatgc ttgtggttct
      481 cctccagttg atagagaaaa acaacccagc gaaggcgcct tctccgaaaa caatgccgag
      541 aatgagagcg gcggagacaa gccccccatc gatcccaata acccggctgc caactggcta
      601 catgctcgct ccactcggaa gaagcgatgc ccctacacaa aacaccagac gctggaactg
      661 gagaaggagt ttctgtttaa catgtacctc acacgggacc gcaggtacga ggtggcccgg
      721 ctgctcaacc tcaccgaaag gcaggtcaag atctggttcc agaaccgcag gatgaaaatg
      781 aagaaaatca acaaggaccg agcaaaagac gagtgagcct tttaggggca catttaaaaa
      841 gagagcaagc tagaaagaaa aagaaaggac tgtccgtctc cctctgtctc ctctccccca
      901 aacccagcct ccacccgcac aaaggggctc taaatcccag gcctcatctc cccactggca
      961 gcccgtgctc aggctggctc ttaggcctgc ggctttgatg gaggaggtat tgtaagcttt
     1021 ccattttata gaaggcacac acacacacaa gggagggcat tagcgctatt ggctgtatgt
     1081 gctagcttgt atatatatat atatatttaa aaaaaatcta cctgcttctg actttaagca
     1141 aaaggaaaga aaactacctt tttatataat gcacaactgt tgatgactgg ctgtatagtt
     1201 tttagtctct gtagctaatt taatttgctc ttcgtgtggc agatcattct gccaaaatac
     1261 ttgaacactg tgttttattg tggtgattat gttttgtgac tcaaacttct gtgctgggtg
     1321 aagtacccat tgtgattgtg gaagctagaa ttcaatttga actcaggttg tttatggggg
     1381 gagagaagct gtggcataat gtatagctct gtagtcttct gtgtagctag actgttaacc
     1441 tacgattgta aactagctgt aaggatttcc cagtgaaatc agatttggaa agaaaagaga
     1501 gatagttctc cgggatgcat agattcatga tttatctact tttgaaatgt gggcttcttc
     1561 atctttacgt tctagactcc aattttgtcc aatgtatata gaatctccat attaaagaaa
     1621 tattcataat cagaaagctt gaatattgtt tttgcaccag aggagaagtg aggaaattcg
     1681 gagctatacg tgtgtgcaga gggttgctac ctatgggttt acgcttaatt ttaattggga
     1741 gaaagtgagt gcttattgta atggagttaa ttttattgat gtaccttata caccatgaaa
     1801 ctgctcttgg gctactgtag atttgtatcc ttggtgaatt tggggttccc atcggattga
     1861 acatacactg tatattttgc aatagttacc tcaaggccta ctgaccaaat tgttgtgtcg
     1921 agatatttaa cttttgccaa acggaatata ttgattcttc tcgagagatc taattctacc
     1981 gggtagggga ggcgcttttc ccaaggcagt ctggagcatg cgctttagca gccccgctgg
     2041 gcacttggcg ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtaggc
     2101 gccaaccggc tccgttcttt ggtggcccct tcgcgccacc ttctactcct cccctagtca
     2161 ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac
     2221 gtctcactag tctcgtgcag atggacagca ccgctgagca atggaagcgg gtaggccttt
     2281 ggggcagcgg ccaatagcag ctttgctcct tcgctttctg ggctcagagg ctgggaaggg
     2341 gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct
     2401 ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt
     2461 cctcatctcc gggcctttcg gctgcagcca ccatgggcag agcaatggtg gccaggctcg
     2521 ggctggggct gctgctgctg gcactgctcc tacccacgca gatttattcc agtgaaacaa
     2581 caactggaac ttcaagtaac tcctcccaga gtacttccaa ctctgggttg gccccaaatc
     2641 caactaatgc caccaccaag gcggctggtg gtgccctgca gtcaacagcc agtctcttcg
     2701 tggtctcact ctctcttctg catctctact cttaaatcga taaaataaaa gattttattt
     2761 agtctccaga aaaagggggg aatgaaagac cccacctgta ggtttggcaa gctagcttaa
     2821 gtaacgccat tttgcaaggc atggaaaata cataactgag aatagagaag ttcagatcaa
     2881 ggttaggaac agagagacag cagaatatgg gccaaacagg atatctgtgg taagcagttc
     2941 ctgccccggc tcagggccaa gaacagatgg tccccagatg cggtcccgcc ctcagcagtt
     3001 tctagagaac catcagatgt ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt
     3061 atttgaacta accaatcagt tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc
     3121 tcaataaaag agcccacaac ccctcactcg gcgcgccagt cctccgatag actgcgtcgc
     3181 ccgggtaccc gtgtatccaa taaaccctct tgcagttgca tccgacttgt ggtctcgctg
     3241 ttccttggga gggtctcctc tgagtgattg actacccgtc agcgggggtc tttcatgggt
     3301 aacagtttct tgaagttgga gaacaacatt ctgagggtag gagtcgaata ttaagtaatc
     3361 ctgactcaat tagccactgt tttgaatcca catactccaa tactcctgaa atagttcatt
     3421 atggacagcg cagaaagagc tggggagaat tgtgaaattg ttatccgctc acaattccac
     3481 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     3541 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     3601 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     3661 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     3721 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     3781 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     3841 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     3901 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     3961 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     4021 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     4081 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     4141 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     4201 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     4261 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     4321 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     4381 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     4441 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     4501 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     4561 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     4621 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     4681 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     4741 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     4801 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     4861 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     4921 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     4981 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     5041 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     5101 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     5161 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     5221 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     5281 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     5341 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     5401 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     5461 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     5521 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     5581 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     5641 cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
     5701 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
     5761 gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga
     5821 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
     5881 accgcatcag gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
     5941 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
     6001 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggcgcaa ggaatggtgc
     6061 atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc
     6121 gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc
     6181 gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc
     6241 gtagaggcga ttagtccaat ttgttaaaga caggatatca gtggtccagg ctctagtttt
     6301 gactcaacaa tatcaccagc tgaagcctat agagtacgag ccatagataa aataaaagat
     6361 tttatttagt ctccagaaaa aggggggaat gaaagacccc acctgtaggt ttggcaagct
     6421 agcttaagta acgccatttt gcaaggcatg gaaaatacat aactgagaat agagaagttc
     6481 agatcaaggt taggaacaga gagacagcag aatatgggcc aaacaggata tctgtggtaa
     6541 gcagttcctg ccccggctca gggccaagaa cagatggtcc ccagatgcgg tcccgccctc
     6601 agcagtttct agagaaccat cagatgtttc cagggtgccc caaggacctg aaatgaccct
     6661 gtgccttatt tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc
     6721 cccgagctca ataaaagagc ccacaacccc tcactcggcg cgccagtcct ccgatagact
     6781 gcgtcgcccg ggtacccgta ttcccaataa agcctcttgc tgtttgcatc cgaatcgtgg
     6841 actcgctgat ccttgggagg gtctcctcag attgattgac tgcccacctc gggggtcttt
     6901 catttggagg ttccaccgag atttggagac ccctgcccag ggaccaccga cccccccgcc
     6961 gggaggtaag ctggccagcg gtcgtttcgt gtctgtctct gtctttgtgc gtgtttgtgc
     7021 cggcatctaa tgtttgcgcc tgcgtctgta ctagttagct aactagctct gtatctggcg
     7081 gacccgtggt ggaactgacg agttctgaac acccggccgc aaccctggga gacgtcccag
     7141 ggactttggg ggccgttttt gtggcccgac ctgaggaagg gagtcgatgt ggaatccgac
     7201 cccgtcagga tatgtggttc tggtaggaga cgagaaccta aaacagttcc cgcctccgtc
     7261 tgaatttttg ctttcggttt ggaaccgaag ccgcgcgtct tgtctgctgc agcgctgcag
     7321 catcgttctg tgttgtctct gtctgactgt gtttctgtat ttgtctgaaa attagggcca
     7381 gactgttacc actcccttaa gtttgacctt aggtcactgg aaagatgtcg agcggatcgc
     7441 tcacaaccag tcggtagatg tcaagaagag acgttgggtt accttctgct ctgcagaatg
     7501 gccaaccttt aacgtcggat ggccgcgaga cggcaccttt aaccgagacc tcatcaccca
     7561 ggttaagatc aaggtctttt cacctggccc gcatggacac ccagaccagg tcccctacat
     7621 cgtgacctgg gaagccttgg cttttgaccc ccctccctgg gtcaagccct ttgtacaccc
     7681 taagcctccg cctcctcttc ctccatccgc cccgtctctc ccccttgaac ctcctcgttc
     7741 gaccccgcct cgatcctccc tttatccagc cctcactcct tctctaggcg ccggaattct
     7801 atggactaca aggacgacga tgacaagctt aagaggcctc ag
//
