LOCUS       sequence-275209-        9123 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  L1 in position 1 (next to LB in L2 construct), for KanR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9123)
  AUTHORS   Sauret-Gueto S, Frangedakis E, Silvestri L, Rebmann M, Tomaselli M, 
            Markel K, Delmans M, West A, Patron NJ, Haseloff J
  TITLE     Systematic tools for reprogramming plant gene expression in a simple
            model, Marchantia polymorpha.
  JOURNAL   ACS Synth Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511.
  PUBMED    32163700
REFERENCE   2  (bases 1 to 9123)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511."
COMMENT     Sequence Label: L1_KanR-Ck1
FEATURES             Location/Qualifiers
     source          1..9123
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1125..1754)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    3055..3079
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     polyA_signal    3100..3274
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(3309..4106)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3420..3439)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4030..4049
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     misc_feature    5868..5892
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6325..7140
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             6325..7140
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(6398..6417)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      7228..7816
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7717..7736
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7970..7987
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8002..8142
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8068..8087)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8228..8250
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8486..8680
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(join(8746..9123,1..696))
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
ORIGIN
        1 ctttacgatc ttgtagcggc taatcaaggc ttcaccctcg gataccgtca ccaggcggcc
       61 gttcttggcc ttcttcgtac gctgcatggc aacgtgcgtg gtgtttaacc gaatgcaggt
      121 ttctaccagg tcgtctttct gctttccgcc atcggctcgc cggcagaact tgagtacgtc
      181 cgcaacgtgt ggacggaaca cgcggccggg cttgtctccc ttcccttccc ggtatcggtt
      241 catggattcg gttagatggg aaaccgccat cagtaccagg tcgtaatccc acacactggc
      301 catgccggcc ggccctgcgg aaacctctac gtgcccgtct ggaagctcgt agcggatcac
      361 ctcgccagct cgtcggtcac gcttcgacag acggaaaacg gccacgtcca tgatgctgcg
      421 actatcgcgg gtgcccacgt catagagcat cggaacgaaa aaatctggtt gctcgtcgcc
      481 cttgggcggc ttcctaatcg acggcgcacc ggctgccggc ggttgccggg attctttgcg
      541 gattcgatca gcggccgctt gccacgattc accggggcgt gcttctgcct cgatgcgttg
      601 ccgctgggcg gcctgcgcgg ccttcaactt ctccaccagg tcatcaccca gcgccgcgcc
      661 gatttgtacc gggccggatg gtttgcgacc gctcacgccg attcctcggg cttgggggtt
      721 ccagtgccat tgcagggccg gcaggcaacc cagccgctta cgcctggcca accgcccgtt
      781 cctccacaca tggggcattc cacggcgtcg gtgcctggtt gttcttgatt ttccatgccg
      841 cctcctttag ccgctaaaat tcatctactc atttattcat ttgctcattt actctggtag
      901 ctgcgcgatg tattcagata gcagctcggt aatggtcttg ccttggcgta ccgcgtacat
      961 cttcagcttg gtgtgatcct ccgccggcaa ctgaaagttg acccgcttca tggctggcgt
     1021 gtctgccagg ctggccaacg ttgcagcctt gctgctgcgt gcgctcggac ggccggcact
     1081 tagcgtgttt gtgcttttgc tcattttctc tttacctcat taactcaaat gagttttgat
     1141 ttaatttcag cggccagcgc ctggacctcg cgggcagcgt cgccctcggg ttctgattca
     1201 agaacggttg tgccggcggc ggcagtgcct gggtagctca cgcgctgcgt gatacgggac
     1261 tcaagaatgg gcagctcgta cccggccagc gcctcggcaa cctcaccgcc gatgcgcgtg
     1321 cctttgatcg cccgcgacac gacaaaggcc gcttgtagcc ttccatccgt gacctcaatg
     1381 cgctgcttaa ccagctccac caggtcggcg gtggcccata tgtcgtaagg gcttggctgc
     1441 accggaatca gcacgaagtc ggctgccttg atcgcggaca cagccaagtc cgccgcctgg
     1501 ggcgctccgt cgatcactac gaagtcgcgc cggccgatgg ccttcacgtc gcggtcaatc
     1561 gtcgggcggt cgatgccgac aacggttagc ggttgatctt cccgcacggc cgcccaatcg
     1621 cgggcactgc cctggggatc ggaatcgact aacagaacat cggccccggc gagttgcagg
     1681 gcgcgggcta gatgggttgc gatggtcgtc ttgcctgacc cgcctttctg gttaagtaca
     1741 gcgataacct tcatgcgttc cccttgcgta tttgtttatt tactcatcgc atcatatacg
     1801 cagcgaccgc atgacgcaag ctgttttact caaatacaca tcaccttttt agacggcggc
     1861 gctcggtttc ttcagcggcc aagctggccg gccaggccgc cagcttggca tcagacaaac
     1921 cggccaggat ttcatgcagc cgcacggttg agaggtgcgc gggcggctcg aacacgtacc
     1981 cggccgcgat catctccgcc tcgatctctt cggtaatgaa aaacggttcg tcctggccgt
     2041 cctggtgcgg tttcatgctt gttcctcttg gcgttcattc tcggcggccg ccagggcgtc
     2101 ggcctcggtc aatgcgtcct cacggaaggc accgcgccgc ctggcctcgg tgggcgtcac
     2161 ttcctcgctg cgctcaagtg cgcggtacag ggtcgagcga tgcacgccaa gcagtgcagc
     2221 cgcctctttc acggtgcggc cttcctggtc gatcagctcg cgggcgtgcg cgatctgtgc
     2281 cggggtgagg gtagggcggg ggccaaactt cacgcctcgg gccttggcgg cctcgcgccc
     2341 gctccgggtg cggtcgatga ttagggaacg ctcgaactcg gcaatgccgg cgaacacggt
     2401 caacaccatg cggccggccg gcgtggtggt gtcggcccac ggctctgcca ggctacgcag
     2461 gcccgcgccg gcctcctgga tgcgctcggc aatgtccagt aggtcgcggg tgctgcgggc
     2521 caggcggtct agcctggtca ctgtcacaac gtcgccaggg cgtaggtggt caagcatcct
     2581 ggccagctcc gggcggtcgc gcctggtgcc ggtgatcttc tcggaaaaca gcttggtgca
     2641 gccggccgcg tgcagttcgg cccgttggtt ggtcaagtcc tggtcgtcgg tgctgacgcg
     2701 ggcatagccc agcaggccag cggcggcgct cttgttcatg gcgtaatgtc tccggttcta
     2761 gtcgcaagta ttctacttta tgcgactaaa acacgcgaca agaaaacgcc aggaaaaggg
     2821 cagggcggca gcctgtcgcg taacttagga cttgtgcgac atgtcgtttt cagatgacgg
     2881 ctgcactgaa cgtcagaagc cgactgcact atagcagcgg aggggttgga tcaaagtact
     2941 ttgatcccga ggggaaccct gtggttggca tgcacataca aatggacgaa cggataaacc
     3001 ttttcacgcc cttttaaata tccgattatt ctaataaacg ctcttttctc ttaggtttac
     3061 ccgccaatat atcctgtcag aattgctctt catgctagcg atctggattt tagtactgga
     3121 ttttggtttt aggaattaga aattttattg atagaagtat tttacaaata caaatacata
     3181 ctaagggttt cttatatgct caacacatga gcgaaaccct ataggaaccc taattccctt
     3241 atctgggaac tactcacaca ttattatgga gaaactcgag cttgtcgatc gactctagct
     3301 agagaagctc agaagaactc gtcaagaagg cgatagaagg cgatgcgctg cgaatcggga
     3361 gcggcgatac cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag ttcttcagca
     3421 atatcacggg tagccaacgc tatgtcctga tagcggtccg ccacacccag ccggccacag
     3481 tcgatgaatc cagaaaagcg gccattttcc accatgatat tcggcaagca ggcatcgcca
     3541 tgtgtcacga cgagatcctc gccgtcgggc atgcgcgcct tgagcctggc gaacagttcg
     3601 gctggcgcga gcccctgatg ttcttcgtcc agatcatcct gatcgacaag accggcttcc
     3661 atccgagtac gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg gcaggtagcc
     3721 ggatcaagcg tatgcagccg ccgcattgca tcagccatga tggatacttt ctcggcagga
     3781 gcaaggtgag atgacaggag atcctgcccc ggcacttcgc ccaatagcag ccagtccctt
     3841 cccgcttcag tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt ggccagccac
     3901 gatagccgcg ctgcctcgtc ctggagttca ttcagggcac cggacaggtc ggtcttgaca
     3961 aaaagaaccg ggcgcccctg cgctgacagc cgaaacacgg cggcatcaga gcagccgatt
     4021 gtctgttgtg cccagtcata gccgaatagc ctctccaccc aagcggccgg agaacctgcg
     4081 tgcaatccat cttgttcaat ccccattggt gacaaccttt ctgcaggcac atcaatactg
     4141 ttgaggcaac acccatagaa gcacggaacc aaggagcacc gacgtccata gcacacagcc
     4201 aacctcgctg accttcgact cctcttcaag tcaaggatag acaaatctat catgcaggaa
     4261 acttacgagg cacctccgca ccagtgaccg aactgaacac cttaccgaga taacaggact
     4321 gaccaaagtg ggatgtactt ggctaaaacg aacctaaaaa atcactcgct cgaggcccca
     4381 atcgacacca cgtaaaagct ccagtcgggt ttcaggtcta gacaatcaca atatcgagca
     4441 atcgtaacga cgtacagaga cgagggcaca aggcgcatac cagaagacaa gatgcagctc
     4501 ctcgatgtcg atgctctcca cgcaagagag aaagaagtga aaggaactct agggttgcgg
     4561 cgatcgattt taagccagac gctccactgc ctcccagcga gctttgagag caagcgcgac
     4621 cacagcacta gacgccaaag tagaagaaat tagggaatga ttagcgaaat tgcggagggt
     4681 tttgatataa ttaaatacct aggtctgccc cgtttataat aacgagcgtt cgataaggac
     4741 tttgcatcgg ctccgttcaa gttattgcga cttagggtcg gcggccggcc attcgcagat
     4801 aggcggccaa ttcagcatgc gagggcaagt tgggaggccg acgacgaagg gttaggaata
     4861 ttcaaagcct cttttccatt tatatattgc cctgtccatg ccccttcgga ctctttgtcg
     4921 tgactgccgg ccagcatcgt catccgaatt gctcacactc ctccgtctgt cacatcgtcc
     4981 gtgcttaggg ccggcacgcc ctttcaattt aacattccaa aataagaaga cgtaggttta
     5041 gcgaacccga ctgctgatac cacaccgatt gctccggttt ttcgagtacc ttttccgcgc
     5101 ttattacaag ctgcgagcaa actttctaga aagtagagag gtggccgaag tcgcttttgg
     5161 cctcccgcgc ctttctcctt ctgcacatac ttccaaacgg tggactagca tcccatagat
     5221 ccatgacatg cacgcaatca catgagattt tactgttgct ttcttcttct ggcctcaccg
     5281 tcctccccgt cctcccttcg ctttttggga cgcgggaaaa cctagaaggc ttctgattga
     5341 agtttccttt tctgcttgtc ggcctttaca ctttggagaa cgaagttcta atctctcttg
     5401 aagttgacca aatattagta tagtgaactg atcgagaaga tgcgggtccg atccaatagg
     5461 agagaacaaa atattacgag acagaaagag ataattaagc aatttatatg cactttaaaa
     5521 tggaatcttt tcgattatca tcaatgagca ttatattgtg aaacggattt aaaggattag
     5581 ctattttgaa aggttttttt aatctgtttt ttaagatctt tagcaaattc tttttaagtt
     5641 acccaaatat cgatacccgt taacaatcaa aaatcgaaga cagagttgtg aatcatcatc
     5701 aagatctgca aatttgatat gattttgaaa atttgagata caaatattgt taaaataatg
     5761 catttaactg ggaaatctat caacttacct tacaattgag ttattttgat atgtctctca
     5821 acaatgtgtg tgactcattt gctcctcatt gaagagcaat tcgttatgtt tacaccacaa
     5881 tatatcctgc caccagccag ccaacagctc cccgaccggc agctcggcac aaaatcacca
     5941 ctcgatacag gcagcccatc agtccgggac ggcgtcagcg ggagagccgt tgtaaggcgg
     6001 cagactttgc tcatgttacc gatgctattc ggaagaacgg caactaagct gccgggtttg
     6061 aaacacggat gatctcgcgg agggtagcat gttgattgta acgatgacag agcgttgctg
     6121 cctgtgatca ccgcggtttc aaaatcggct ccgtcgatac tatgttatac gccaactttg
     6181 aaaacaactt tgaaaaagct gttttctggt atttaaggtt ttagaatgca aggaacagtg
     6241 aattggagtt cgtcttgtta taattagctt cttggggtat ctttaaatac tgtagaaaag
     6301 aggaaggaaa taataagttc tgttatgagc catattcaac gggaaacgtc ttgctcaagg
     6361 ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat
     6421 gtcgggcaat caggtgcgac aatctaccga ttgtatggga agcccgatgc gccagagttg
     6481 tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta
     6541 aactggctga cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat
     6601 gatgcatggt tactcaccac tgcgatccca gggaaaacag cattccaggt attagaagaa
     6661 tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat
     6721 tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgact cgctcaggcg
     6781 caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc
     6841 tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc accggattca
     6901 gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata
     6961 ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta
     7021 tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt
     7081 attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa
     7141 tcacctagaa tgcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     7201 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     7261 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     7321 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     7381 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     7441 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     7501 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     7561 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     7621 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     7681 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     7741 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     7801 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     7861 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     7921 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     7981 gagcgaggaa gcggatgagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     8041 ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     8101 gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca
     8161 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     8221 accgtcaccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg
     8281 cagggtgcct tgatgtgggc gccggcggtc gagtggcgac ggcgcggctt gtccgcgccc
     8341 tggtagattg cctggccgta ggccagccat ttttgagcgg ccagcggccg cgataggccg
     8401 acgcgaagcg gcggggcgta gggagcgcag cgaccgaagg gtaggcgctt tttgcagcac
     8461 ttcggctgtg cgctggccag acagttatgc acaggccagg cgggttttaa gagttttaat
     8521 aagttttaaa gagttttagg cggaaaaatc gccttttttc tcttttatat cagtcactta
     8581 catgtgtgac cggttcccaa tgtacggctt tgggttccca atgtacgggt tccggttccc
     8641 aatgtacggc tttgggttcc caatgtacgt gctatccaca ggaaagagtc cttttcgacc
     8701 tttttcccct gctagggcaa tttgccctag catctgctcc gtacattagg aaccggcgga
     8761 tgcttcgccc tcgatcaggt tgcggtagcg catgactagg atcgggccag cctgccccgc
     8821 ctcctccttc aaatcgtact ccggcaggtc atttgacccg atcagcttgc gcacggtgaa
     8881 acagaacttc ttgaactctc cggcgctgcc actgcgttcg tagatcgtct tgaacaacca
     8941 tctggcttct gccttgcctg cggcgcggcg tgccaggcgg tagagaaaac ggccgatgcc
     9001 gggatcgatc aaaaagtaat cggggtgaac cgtcagcacg tccgggttct tgccttctgt
     9061 gatctcgcgg tacatccaat cagctagctc gatctcgatg tactccggcc gcccggtttc
     9121 gct
//
