LOCUS       sequence-275210-       13519 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  L1 in position 2, example of weak promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13519)
  AUTHORS   Sauret-Gueto S, Frangedakis E, Silvestri L, Rebmann M, Tomaselli M, 
            Markel K, Delmans M, West A, Patron NJ, Haseloff J
  TITLE     Systematic tools for reprogramming plant gene expression in a simple
            model, Marchantia polymorpha.
  JOURNAL   ACS Synth Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511.
  PUBMED    32163700
REFERENCE   2  (bases 1 to 13519)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511."
COMMENT     Sequence Label: L1_NOP1:mS-N7-Ck2
FEATURES             Location/Qualifiers
     source          1..13519
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    320..344
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6061..6756
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet-I
                     /note="matures faster than mScarlet"
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     6462..6481
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     terminator      7035..7287
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     polyA_signal    7334..7508
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    7529..7553
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             8854..9483
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             9912..10985
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      11051..11245
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(11481..11503)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    11589..11729
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     11644..11663
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(11744..11761)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11915..12503)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11995..12014)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(12591..13406)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(12591..13406)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     13314..13333
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 taccagaaaa cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg
       61 gagccgattt tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa
      121 catgctaccc tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc
      181 cgaatagcat cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc
      241 cgtcccggac tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg
      301 ggagctgttg gctggctggt ggcaggatat attgtggtgt aaacataacg aattgctctt
      361 cagcaaggag ttctcggaag gtcgctaaat cacagggttg tcattggaca ttcgcaggga
      421 cgaactggaa gccgagagag gaatctgtgc ggaatctcag actcgaagag acgtcgtgga
      481 agcatatcgt agaggatttc tttggcgtgt gaaggaatcg acggatggag agcacgcatg
      541 attgttcgag cgcaagatta cggctcactc atccgatcca accaggcata gatggcggta
      601 gacgctctac tgggttccag aaacgtcgct gattgcacag agttctgttg attgttgatg
      661 acacgagctt tgaaatgcga tcgtctcaca gtcacaacat cttcctgatc caacacgtca
      721 tacttctccc tagcgacgtc gcgactgtcc tcgggctgac tccattgcag cgaaatgtcg
      781 ttcccagcac atagttcgag ctcaactgga tgtccttata aggaactgca cattttcaga
      841 cgagagcatt tcattagaga cgtttccgca tgatattgtt taaacatcaa catggcatcg
      901 taaacattga gcaatttaaa gtgaatagaa tgatgcagct gcataattca ttgttagtaa
      961 catttatttt ttgcaatatc tcatgtgacg gatgtagcac tattcaatgt ggacagaaat
     1021 gacaaaatta gccatcaaat gcatttttta atttctcact ctgatcttaa ttactaatat
     1081 aattgggcat cttagtcact ccatatctga aaccagctta gttttcatat attttcaaga
     1141 tttcagcgcg aaatattatg ttcaaaagaa gaaagacgaa gaatagcttt cggcttctgt
     1201 ttctttgttc ggttaaggtt atggataatt cccaggtttg gggtctacaa tacgttcata
     1261 tcgtgtacaa gttctgtttc aatgagagga attccccaca ttatgattca taccttcact
     1321 caaccttcaa tgaaatatga aaataaaatt ataagttttc tgaacttgag aatttgtata
     1381 caaataaaag gggtaaggta gattaatata atgatgttga tggatgatgg caaatatagg
     1441 atgatctaca tcatgatttt ctccatgatc acgttatatt tatctgcaac aaacttgaag
     1501 tatatctgta ttatgaactt tttcttttga gaagtggaaa caaattttga tgtaaatttt
     1561 ctactaaatt gatataaatg aatcatttac atatcatcat tatcctatca atctagtact
     1621 tatttacata aatattaaaa tttaaattgt tctcactctt tgagcaatga cgtatcagtt
     1681 gcactctggt ttataaccca gtctgatatt ttagttacaa acatggaaga tcaaatacca
     1741 atgctcttat tttccactgt aataagtttt ttacttctgg attaaattca ttgatcattt
     1801 ttttaaactt tcatgtcctt gattaacttt ctgcaaaaaa gatgcgaatg tattcatcgc
     1861 ggtaatatta ttgtgtctta tttgattcat tttctcgtaa aatataattc aaaatttaag
     1921 ttagtaaatt atttcttata cgttggtaga tataaatgca gatgtatatc ttagtcacta
     1981 tgatatacat tgaaggacat gtgaataatt taacactgca aatgttccga aaccagaatt
     2041 tagggttata ctgatactga taaaataaat ttgtttttaa ctattatatt tcatttagga
     2101 tcgatatact tattaagttg agtatcatga aaaaaactcg tatgaaaaaa gaatgaagca
     2161 attatcaagt agatcgatgt taagtgaaat cttgattaca aatataatta tctagaagaa
     2221 gaataaggac ctgacagtgt tggattttta agcttataaa aatgcaatga acgactgttt
     2281 gttccatgtt tggtttcgaa tctacttact cacttcatga taggctagaa atatatctcc
     2341 ctttaaagga ttgataaata tctatatttg atgtccaaga aaagttcagc gggggctata
     2401 caggtattgt ataactttct tgccgagtca ttcccaagat gtatttatga agagtaagac
     2461 gagtgacgag cgtattttct acacggaaag tgttaacttt taggtcaaga atgcaccccc
     2521 agttgcggcc ggcggtgcag tattaattcg gtgctccggt tgtctctgga gtcctataca
     2581 agaagtatcc gatcaaaata catgacctgg ggatcctagc cccactcgag cgctaggctt
     2641 gcctctatca tgtcatgtaa atatctcttt acagatgcac gcccggtcga taaagctaaa
     2701 atacttgcac actttacagg aggatatacc tgcgatggac gtgattatgt caaagggaat
     2761 tcggtccccc gcgtacgagg cgggcgaaga gacttggtgc gccatccaca tatccgaacg
     2821 cactgcccca gaaatgggtg caaatccaat gccacgtcag gacacagggt ccacgtagta
     2881 aagtccaatt gggtgcaggc cgcgacgtcg agcaggaacg gcatgcacgc aggatccgac
     2941 agcaggcgtc cggattggat tggacaatga tgacggacgc ccgccttggg gcttgggttg
     3001 gggtcacggc ttccacctga cgacggggaa catggaagga aggaaggacg gaaggaagcg
     3061 gcaggccggg ggcggaagaa tctacgacgg cgacgcagca gtcgaagggc agggcacgga
     3121 aagggggcgc ggccagcgag cgagggaacg agaggaaagg cgaaatactg aaaatggcgt
     3181 gaggcacaaa agtccgcatt aaaataataa aggcctcgaa gcgcaggagt gcgggccagg
     3241 cggggcagcg gaagctgcag gagcatccgc gagcgatgcg cgtgtcaaag caattgaata
     3301 agtcattgag agcttggctc gatggcgata ctggcttgaa ccttgtgact tggctgccta
     3361 gctgtccatt ttgtccgact ggttctcctg ggccttgatg gtgcacacaa tggagtccgt
     3421 gcgagtcagt gggcgggcag acacagctag agcccccgca ctgtgagcag ccttcaaggg
     3481 aaggccaggg gccgagagaa atgcgctcca acggcccggc cccgatggga tggcatggca
     3541 tgggaggagg acgagcagca gctggttcgc ctggaggtga agaagagtag aggtggagtt
     3601 tagtgagtcc agttcacaga ggagggcgcc ggaaaagact cctgactcat gactgggatc
     3661 agacacatga tggcggatga cctttctgcc ttcctgcgca tgactctgag ctcaagtctc
     3721 atcttgtagt cgaggtgagc gaatgactgt gtatgctcgt agtttacatg tttgttgctc
     3781 ggccttcgtt ctgcctcaag ttcagcagca agctcagtcc tgggctcagc ttcccccgtc
     3841 tcctctctct ctctatctcc ctccctcgtt tctatttcct ctctcgtggt cttcggtccc
     3901 gttcggtctg ttctgtaggg tggatgctat tatggtctgt tgaagggcat catgggatgg
     3961 gttggattgg gacgagcagc tggaatggag tgagtctgaa gctcttattc gggctcgcct
     4021 cccgctcttg gagctgcaaa cgaagccgcg cagcgtcgtg cgtcagctcc acaagtctgg
     4081 gacggaggga ttgatgcata tttactaagt ttacagcccc ttcggttgga tcctcgtcgg
     4141 cctctgtcat cattcgttct gttcgttcgt ccgaggcggt gctgtgaaag aacgggtcgt
     4201 cgctgggttg ggttgggttg actttcgggc caatttgcga gccccgaacc ggggacggac
     4261 ctggctgggc cttgattctt caattcggcc ttgaccacgt cacgccctcc cgcttgtcct
     4321 tgctcgctgc ctcgaccccg cccggacagc tagaacgaga ttcctggagc ccgcaggtgg
     4381 agcttatctc ctcctccccg cgcactccga attttcctcg cctctgctgc ctcggactgt
     4441 cgctcgtccc gcccgcgccc aggagacgag gtcccgtgcc atctctcgtc tgtgtctctc
     4501 tcggtcctgt tgtgctgctc tcttggccac tggaggcgag caagcgagca agcgagaaag
     4561 gaaggaagta aggataggtg cctgacggcg gacggagccg cagctggagc tggggtcgtt
     4621 cttcttcttt gcgtgtaggg agacgacgaa gtgccggcat tgtagagatc agcggtgtca
     4681 cactcacgcc tgtgcgaata gtatcggaat gaaataggtt tatgcttgag ctctggggcg
     4741 agggggcttt ctgcattgag ttgagcgtcg gcccgagaag gcggggaggc aggcagtcca
     4801 ccgaggtcgg ccatagcttt ccttcccaac ctcgtatgtg ctcgccggaa tgaaggaaga
     4861 gaatgatcat gatactgccg agatgttgca agagcccgtg gtgggatcag agcatggagt
     4921 ttgatttttg gtaagcagta tgagttatgg ttcagagagt ggaaggtgga aagggtgagg
     4981 gagtggcagt gagcacagca gggtcgagca ggctatgtag ctggaatgga gtgcgagcaa
     5041 gctcaagagt tttctccttt ggattttccg gtagatttgt aatgctcgag agtagaaatt
     5101 ccaggccaac tccctcattt gatgtcccga ggggagcaag ggtcttcttc tcatgggaac
     5161 ggcggtcaaa ttctcggtgg attgaaacgg ggcctagctg ggaatttctc atcgaaagaa
     5221 attttcagcg ctgaaatacg tccgagagat caagtaaggt tttgataaca gtgcagaagg
     5281 ggatattgtc tggtgggttg ggattgttgc tggcgcagtc aaacgaggga aatactccta
     5341 agcgtggttc tgctgtcttt gtcatatttc aaagtcttca tctgtttttc atcacccagc
     5401 gagtgtgaga agtctgaatg tgtgagcaga aataaaaggg aatttcgagc cataagaacc
     5461 agctaaagaa catcaaacga gctgtgagcg gactcgtctc ttagacttaa gattcactca
     5521 aacgtcattg cgtgtaactt gttcctagga ctgctttttt ggtttgctgc cgtcgatatc
     5581 gtcacacgtt ttgtagtctt ccattctcgt gattaccccg gaattatttt cgtcttcacc
     5641 tgaacgattt tcttacaaag tggacgagat gaacgtgctt gttctctcga gggccccgga
     5701 tcgaccttct gctttttcgt cctcgtttga tctcctggga tccgagagct tggtcactcg
     5761 tcgatgtttt cctcgttaga aaaagtgtaa tgggttagca gtcccctgca tcagactgtc
     5821 aatttccatt tgttttaatt ggaagaaggc ccggagtttc ccgggttcgt ctttactctg
     5881 ttcgtatagt gtcgcgtgtg cccagttgtt gtatttccag agtgtacctt gcggtggagt
     5941 tgactttttc gtgccacatg aatgtaaacc gtttgagcac tattatctgt taaggctgct
     6001 aatcaacact gacctctcaa ttgcgctgta tgtggatgca gttaggagcc ttgtcgaata
     6061 atggtgagca agggcgaggc agtgatcaag gagttcatgc ggttcaaggt gcacatggag
     6121 ggctccatga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
     6181 ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt ctcctgggac
     6241 atcctgtccc ctcagttcat gtacggctcc agggccttca tcaagcaccc cgccgacatc
     6301 cccgactact ataagcagtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
     6361 gaggacggcg gcgccgtgac cgtgacccag gacacctccc tggaggacgg caccctgatc
     6421 tacaaggtga agctccgcgg caccaacttc cctcctgacg gccccgtaat gcagaagaag
     6481 acaatgggct gggaagcgtc caccgagcgg ttgtaccccg aggacggcgt gctgaagggc
     6541 gacattaaga tggccctgcg cctgaaggac ggcggccgct acctggcgga cttcaagacc
     6601 acctacaagg ccaagaagcc cgtgcagatg cccggcgcct acaacgtcga ccgcaagttg
     6661 gacatcacct cccacaacga ggactacacc gtggtggaac agtacgaacg ctccgagggc
     6721 cgccactcca ccggcggcat ggacgagctg tacaaggctt cggctgcagc ggccgaattc
     6781 aagcgtgaag aacaagcaag gaaagctaag gtgaacaatg agaaaaagac ggaaatagtg
     6841 aaaccagaga gttgtagcaa tgaaggagat gtcaaggatc tgaaaagaaa ggactctgag
     6901 gatggaaacg agggtgagga agaagaagct tcttcgaaac cgaaaaagcc aaaagttgct
     6961 ctttctcatc ttcaggacat tgacgacaca gaagctgatc aagaagaaga gtaagcttga
     7021 gctcgaattt ccccgatcgt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg
     7081 ttgccggtct tgcgatgatt atcatataat ttctgttgaa ttacgttaag catgtaataa
     7141 ttaacatgta atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat
     7201 tatacattta atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc
     7261 gcgcggtgtc atctatgtta ctagatcgat ccgtatcgat agcctctagc tagagtcgat
     7321 cgacaagctc gagtttctcc ataataatgt gtgagtagtt cccagataag ggaattaggg
     7381 ttcctatagg gtttcgctca tgtgttgagc atataagaaa cccttagtat gtatttgtat
     7441 ttgtaaaata cttctatcaa taaaatttct aattcctaaa atcaaaatcc agtactaaaa
     7501 tccagatcgc tatactgaag agcaattctg acaggatata ttggcgggta aacctaagag
     7561 aaaagagcgt ttattagaat aatcggatat ttaaaagggc gtgaaaaggt ttatccgttc
     7621 gtccatttgt atgtgcatgc caaccacagg gttcccctcg ggatcaaagt actttgatcc
     7681 aacccctccg ctgctatagt gcagtcggct tctgacgttc agtgcagccg tcatctgaaa
     7741 acgacatgtc gcacaagtcc taagttacgc gacaggctgc cgccctgccc ttttcctggc
     7801 gttttcttgt cgcgtgtttt agtcgcataa agtagaatac ttgcgactag aaccggagac
     7861 attacgccat gaacaagagc gccgccgctg gcctgctggg ctatgcccgc gtcagcaccg
     7921 acgaccagga cttgaccaac caacgggccg aactgcacgc ggccggctgc accaagctgt
     7981 tttccgagaa gatcaccggc accaggcgcg accgcccgga gctggccagg atgcttgacc
     8041 acctacgccc tggcgacgtt gtgacagtga ccaggctaga ccgcctggcc cgcagcaccc
     8101 gcgacctact ggacattgcc gagcgcatcc aggaggccgg cgcgggcctg cgtagcctgg
     8161 cagagccgtg ggccgacacc accacgccgg ccggccgcat ggtgttgacc gtgttcgccg
     8221 gcattgccga gttcgagcgt tccctaatca tcgaccgcac ccggagcggg cgcgaggccg
     8281 ccaaggcccg aggcgtgaag tttggccccc gccctaccct caccccggca cagatcgcgc
     8341 acgcccgcga gctgatcgac caggaaggcc gcaccgtgaa agaggcggct gcactgcttg
     8401 gcgtgcatcg ctcgaccctg taccgcgcac ttgagcgcag cgaggaagtg acgcccaccg
     8461 aggccaggcg gcgcggtgcc ttccgtgagg acgcattgac cgaggccgac gccctggcgg
     8521 ccgccgagaa tgaacgccaa gaggaacaag catgaaaccg caccaggacg gccaggacga
     8581 accgtttttc attaccgaag agatcgaggc ggagatgatc gcggccgggt acgtgttcga
     8641 gccgcccgcg cacctctcaa ccgtgcggct gcatgaaatc ctggccggtt tgtctgatgc
     8701 caagctggcg gcctggccgg ccagcttggc cgctgaagaa accgagcgcc gccgtctaaa
     8761 aaggtgatgt gtatttgagt aaaacagctt gcgtcatgcg gtcgctgcgt atatgatgcg
     8821 atgagtaaat aaacaaatac gcaaggggaa cgcatgaagg ttatcgctgt acttaaccag
     8881 aaaggcgggt caggcaagac gaccatcgca acccatctag cccgcgccct gcaactcgcc
     8941 ggggccgatg ttctgttagt cgattccgat ccccagggca gtgcccgcga ttgggcggcc
     9001 gtgcgggaag atcaaccgct aaccgttgtc ggcatcgacc gcccgacgat tgaccgcgac
     9061 gtgaaggcca tcggccggcg cgacttcgta gtgatcgacg gagcgcccca ggcggcggac
     9121 ttggctgtgt ccgcgatcaa ggcagccgac ttcgtgctga ttccggtgca gccaagccct
     9181 tacgacatat gggccaccgc cgacctggtg gagctggtta agcagcgcat tgaggtcacg
     9241 gatggaaggc tacaagcggc ctttgtcgtg tcgcgggcga tcaaaggcac gcgcatcggc
     9301 ggtgaggttg ccgaggcgct ggccgggtac gagctgccca ttcttgagtc ccgtatcacg
     9361 cagcgcgtga gctacccagg cactgccgcc gccggcacaa ccgttcttga atcagaaccc
     9421 gagggcgacg ctgcccgcga ggtccaggcg ctggccgctg aaattaaatc aaaactcatt
     9481 tgagttaatg aggtaaagag aaaatgagca aaagcacaaa cacgctaagt gccggccgtc
     9541 cgagcgcacg cagcagcaag gctgcaacgt tggccagcct ggcagacacg ccagccatga
     9601 agcgggtcaa ctttcagttg ccggcggagg atcacaccaa gctgaagatg tacgcggtac
     9661 gccaaggcaa gaccattacc gagctgctat ctgaatacat cgcgcagcta ccagagtaaa
     9721 tgagcaaatg aataaatgag tagatgaatt ttagcggcta aaggaggcgg catggaaaat
     9781 caagaacaac caggcaccga cgccgtggaa tgccccatgt gtggaggaac gggcggttgg
     9841 ccaggcgtaa gcggctgggt tgcctgccgg ccctgcaatg gcactggaac ccccaagccc
     9901 gaggaatcgg cgtgagcggt cgcaaaccat ccggcccggt acaaatcggc gcggcgctgg
     9961 gtgatgacct ggtggagaag ttgaaggccg cgcaggccgc ccagcggcaa cgcatcgagg
    10021 cagaagcacg ccccggtgaa tcgtggcaag cggccgctga tcgaatccgc aaagaatccc
    10081 ggcaaccgcc ggcagccggt gcgccgtcga ttaggaagcc gcccaagggc gacgagcaac
    10141 cagatttttt cgttccgatg ctctatgacg tgggcacccg cgatagtcgc agcatcatgg
    10201 acgtggccgt tttccgtctg tcgaagcgtg accgacgagc tggcgaggtg atccgctacg
    10261 agcttccaga cgggcacgta gaggtttccg cagggccggc cggcatggcc agtgtgtggg
    10321 attacgacct ggtactgatg gcggtttccc atctaaccga atccatgaac cgataccggg
    10381 aagggaaggg agacaagccc ggccgcgtgt tccgtccaca cgttgcggac gtactcaagt
    10441 tctgccggcg agccgatggc ggaaagcaga aagacgacct ggtagaaacc tgcattcggt
    10501 taaacaccac gcacgttgcc atgcagcgta cgaagaaggc caagaacggc cgcctggtga
    10561 cggtatccga gggtgaagcc ttgattagcc gctacaagat cgtaaagagc gaaaccgggc
    10621 ggccggagta catcgagatc gagctagctg attggatgta ccgcgagatc acagaaggca
    10681 agaacccgga cgtgctgacg gttcaccccg attacttttt gatcgatccc ggcatcggcc
    10741 gttttctcta ccgcctggca cgccgcgccg caggcaaggc agaagccaga tggttgttca
    10801 agacgatcta cgaacgcagt ggcagcgccg gagagttcaa gaagttctgt ttcaccgtgc
    10861 gcaagctgat cgggtcaaat gacctgccgg agtacgattt gaaggaggag gcggggcagg
    10921 ctggcccgat cctagtcatg cgctaccgca acctgatcga gggcgaagca tccgccggtt
    10981 cctaatgtac ggagcagatg ctagggcaaa ttgccctagc aggggaaaaa ggtcgaaaag
    11041 gactctttcc tgtggatagc acgtacattg ggaacccaaa gccgtacatt gggaaccgga
    11101 acccgtacat tgggaaccca aagccgtaca ttgggaaccg gtcacacatg taagtgactg
    11161 atataaaaga gaaaaaaggc gatttttccg cctaaaactc tttaaaactt attaaaactc
    11221 ttaaaacccg cctggcctgt gcataactgt ctggccagcg cacagccgaa gtgctgcaaa
    11281 aagcgcctac ccttcggtcg ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg
    11341 cggccgctgg ccgctcaaaa atggctggcc tacggccagg caatctacca gggcgcggac
    11401 aagccgcgcc gtcgccactc gaccgccggc gcccacatca aggcaccctg cctcgcgcgt
    11461 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggtgacggt cacagcttgt
    11521 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
    11581 tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact
    11641 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
    11701 gatgcgtaag gagaaaatac cgcatcaggc gctcatccgc ttcctcgctc actgactcgc
    11761 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
    11821 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
    11881 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
    11941 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
    12001 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
    12061 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
    12121 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
    12181 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
    12241 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
    12301 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
    12361 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
    12421 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
    12481 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
    12541 agtggaacga aaactcacgt taagggattt tggtcatgca ttctaggtga ttagaaaaac
    12601 tcatcgagca tcaaatgaaa ctgcaattta ttcatatcag gattatcaat accatatttt
    12661 tgaaaaagcc gtttctgtaa tgaaggagaa aactcaccga ggcagttcca taggatggca
    12721 agatcctggt atcggtctgc gattccgact cgtccaacat caatacaacc tattaatttc
    12781 ccctcgtcaa aaataaggtt atcaagtgag aaatcaccat gagtgacgac tgaatccggt
    12841 gagaatggca aaagtttatg catttctttc cagacttgtt caacaggcca gccattacgc
    12901 tcgtcatcaa aatcactcgc atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg
    12961 agtcgaaata cgcgatcgct gttaaaagga caattacaaa caggaatcga atgcaaccgg
    13021 cgcaggaaca ctgccagcgc atcaacaata ttttcacctg aatcaggata ttcttctaat
    13081 acctggaatg ctgttttccc tgggatcgca gtggtgagta accatgcatc atcaggagta
    13141 cggataaaat gcttgatggt cggaagaggc ataaattccg tcagccagtt tagtctgacc
    13201 atctcatctg taacatcatt ggcaacgcta cctttgccat gtttcagaaa caactctggc
    13261 gcatcgggct tcccatacaa tcggtagatt gtcgcacctg attgcccgac attatcgcga
    13321 gcccatttat acccatataa atcagcatcc atgttggaat ttaatcgcgg ccttgagcaa
    13381 gacgtttccc gttgaatatg gctcataaca gaacttatta tttccttcct cttttctaca
    13441 gtatttaaag ataccccaag aagctaatta taacaagacg aactccaatt cactgttcct
    13501 tgcattctaa aaccttaaa
//
