LOCUS       sequence-275215-       10640 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10640)
  AUTHORS   Park BS, Song DH, Kim HM, Choi BS, Lee H, Lee JO
  TITLE     The structural basis of lipopolysaccharide recognition by the 
            TLR4-MD-2 complex.
  JOURNAL   Nature. 2009 Apr 30;458(7242):1191-5. doi: 10.1038/nature07830. Epub
            2009 Mar 1.
  PUBMED    19252480
REFERENCE   2  (bases 1 to 10640)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature07830"; journalName: "Nature"; date: "2009-04-30- 
            30"; volume: "458"; issue: "7242"; pages: "1191-5"
COMMENT     Sequence Label: MD-2
FEATURES             Location/Qualifiers
     source          1..10640
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     3173..4000
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             3173..3297
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(3335..3940)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     promoter        4004..4095
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     4020..4037
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     sig_peptide     4192..4251
                     /codon_start=1
                     /gene="Autographa californica gp64"
                     /product="signal sequence from the baculovirus envelope 
                     glycoprotein GP64"
                     /label=GP64 signal sequence
                     /translation="MVSAIVLYVLLAAAAHSAFA"
     CDS             4693..4710
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             4741..4914
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             4915..5088
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             5127..5144
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             5145..5162
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_recomb     5651..6356
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     3'UTR           5739..6111
                     /label=polyhedrin 3' UTR
                     /note="3' UTR from the Autographa californica 
                     nucleopolyhedrovirus polyhedrin gene (Suzuki et al., 2006)"
     CDS             complement(5768..6356)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="IPKSSTTNLIADVLADTINRRRVAMAKSSSEATSNDEGWDDDDNR
                     PNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLLQNKTQTNIDKAR
                     LLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIFKFRYKDAENHLIF
                     ALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     CDS             complement(6790..6807)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(8024..8040)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8024..8040)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8037..8059)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8048..8064
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8072..8078,8079..8096,8097..8102))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8117..8138
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8255..8272)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8426..9014)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8506..8525)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9185..9976,9977..10045))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9808..9827
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10046..10150)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10218..10236
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10274..10296)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10396..10415
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10609..10631
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10623..10640
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10624..10640
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gccaagcttg gctcgtaaag cgagttgaag gatcatattt agttgcgttt atgagataag
       61 attgaaagca cgtgtaaaat gtttcccgcg cgttggcaca actatttaca atgcggccaa
      121 gttataaaag attctaatct gatatgtttt aaaacacctt tgcggcccga gttgtttgcg
      181 tacgtgacta gcgaagaaga tatgtggacc gcagaacaga tagtaaaaca aaaccctagt
      241 attggagcaa taatcgattt aaccaacacg tctaaatatt atgatggtgt gcattttttg
      301 cgggcgggcc tgttatacaa aaaaattcaa gtacctggcc agactttgcc gcctgaaagc
      361 atagttcaag aatttattga cacggtaaaa gaatttacag aaaagtgtcc cggcatgttg
      421 gtgggcgtgc actgcacaca cggtattaat cgcaccggtt acatggtgtg cagatattta
      481 atgcacaccc tgggtattgc gccgcaggaa gccatagata gattcgaaaa agccagaggt
      541 cacaaaattg aaagacaaaa ttacgttcaa gatttattaa tttaattaat attatttgca
      601 ttctttaaca aatactttat cctattttca aattgttgcg cttcttccag cgaaccaaaa
      661 ctatgcttcg cttgctccgt ttagcttgta gccgatcagt ggcgttgttc caatcgacgg
      721 taggattagg ccggatattc tccaccacaa tgttggcaac gttgatgtta cgtttatgct
      781 tttggttttc cacgtacgtc ttttggccgg taatagccgt aaacgtagtg ccgtcgcgcg
      841 tcacgcacaa caccggatgt ttgcgcttgt ccgcggggta ttgaaccgcg cgatccgaca
      901 aatccaccac tttggcaact aaatcggtga cctgcgcgtc ttttttctgc attatttcgt
      961 ctttcttttg catggtttcc tggaagccgg tgtacatgcg gtttagatca gtcatgacgc
     1021 gcgtgacctg caaatctttg gcctcgatct gcttgtcctt gatggcaacg atgcgttcaa
     1081 taaactcttg ttttttaaca agttcctcgg ttttttgcgc caccaccgct tgcagcgcgt
     1141 ttgtgtgctc ggtgaatgtc gcaatcagct tagtcaccaa ctgtttgctc tcctcctccc
     1201 gttgtttgat cgcgggatcg tacttgccgg tgcagagcac ttgaggaatt acttcttcta
     1261 aaagccattc ttgtaattct atggcgtaag gcaatttgga cttcataatc agctgaatca
     1321 cgccggattt agtaatgagc actgtatgcg gctgcaaata cagcgggtcg ccccttttca
     1381 cgacgctgtt agaggtaggg cccccatttt ggatggtctg ctcaaataac gatttgtatt
     1441 tattgtctac atgaacacgt atagctttat cacaaactgt atattttaaa ctgttagcga
     1501 cgtccttggc cacgaaccgg acctgttggt cgcgctctag cacgtaccgc aggttgaacg
     1561 tatcttctcc aaatttaaat tctccaattt taacgcgagc cattttgata cacgtgtgtc
     1621 gattttgcaa caactattgt tttttaacgc aaactaaact tattgtggta agcaataatt
     1681 aaatatgggg gaacatgcgc cgctacaaca ctcgtcgtta tgaacgcaga cggcgccggt
     1741 ctcggcgcaa gcggctaaaa cgtgttgcgc gttcaacgcg gcaaacatcg caaaagccaa
     1801 tagtacagtt ttgatttgca tattaacggc gattttttaa attatcttat ttaataaata
     1861 gttatgacgc ctacaactcc ccgcccgcgt tgactcgctg cacctcgagc agttcgttga
     1921 cgccttcctc cgtgtggccg aacacgtcga gcgggtggtc gatgaccagc ggcgtgccgc
     1981 acgcgacgca caagtatctg tacaccgaat gatcgtcggg cgaaggcacg tcggcctcca
     2041 agtggcaata ttggcaaatt cgaaaatata tacagttggg ttgtttgcgc atatctatcg
     2101 tggcgttggg catgtacgtc cgaacgttga tttgcatgca agccgaaatt aaatcattgc
     2161 gattagtgcg attaaaacgt tgtacatcct cgcttttaat catgccgtcg attaaatcgc
     2221 gcaatcgagt caagtgatca aagtgtggaa taatgttttc tttgtattcc cgagtcaagc
     2281 gcagcgcgta ttttaacaaa ctagccatct tgtaagttag tttcatttaa tgcaacttta
     2341 tccaataata tattatgtat cgcacgtcaa gaattaacaa tgcgcccgtt gtcgcatctc
     2401 aacacgacta tgatagagat caaataaagc gcgaattaaa tagcttgcga cgcaacgtgc
     2461 acgatctgtg cacgcgttcc ggcacgagct ttgattgtaa taagttttta cgaagcgatg
     2521 acatgacccc cgtagtgaca acgatcacgc ccaaaagaac tgccgactac aaaattaccg
     2581 agtatgtcgg tgacgttaaa actattaagc catccaatcg accgttagtc gaatcaggac
     2641 cgctggtgcg agaagccgcg aagtatggcg aatgcatcgt ataacgtgtg gagtccgctc
     2701 attagagcgt catgtttaga caagaaagct acatatttaa ttgatcccga tgattttatt
     2761 gataaattga ccctaactcc atacacggta ttctacaatg gcggggtttt ggtcaaaatt
     2821 tccggactgc gattgtacat gctgttaacg gctccgccca ctattaatga aattaaaaat
     2881 tccaatttta aaaaacgcag caagagaaac atttgtatga aagaatgcgt agaaggaaag
     2941 aaaaatgtcg tcgacatgct gaacaacaag attaatatgc ctccgtgtat aaaaaaaata
     3001 ttgaacgatt tgaaagaaaa caatgtaccg cgcggcggta tgtacaggaa gaggtttata
     3061 ctaaactgtt acattgcaaa cgtggtttcg tgtgccaagt gtgaaaaccg atgtttaatc
     3121 aaggctctga cgcatttcta caaccacgac tccaagtgtg tgggtgaagt catgcatctt
     3181 ttaatcaaat cccaagatgt gtataaacca ccaaactgcc aaaaaatgaa aactgtcgac
     3241 aagctctgtc cgtttgctgg caactgcaag ggtctcaatc ctatttgtaa ttattgaata
     3301 ataaaacaat tataaatgtc aaatttgttt tttattaacg atacaaacca aacgcaacaa
     3361 gaacatttgt agtattatct ataattgaaa acgcgtagtt ataatcgctg aggtaatatt
     3421 taaaatcatt ttcaaatgat tcacagttaa tttgcgacaa tataatttta ttttcacata
     3481 aactagacgc cttgtcgtct tcttcttcgt attccttctc tttttcattt ttctcctcat
     3541 aaaaattaac atagttatta tcgtatccat atatgtatct atcgtataga gtaaattttt
     3601 tgttgtcata aatatatatg tcttttttaa tggggtgtat agtaccgctg cgcatagttt
     3661 ttctgtaatt tacaacagtg ctattttctg gtagttcttc ggagtgtgtt gctttaatta
     3721 ttaaatttat ataatcaatg aatttgggat cgtcggtttt gtacaatatg ttgccggcat
     3781 agtacgcagc ttcttctagt tcaattacac cattttttag cagcaccgga ttaacataac
     3841 tttccaaaat gttgtacgaa ccgttaaaca aaaacagttc acctcccttt tctatactat
     3901 tgtctgcgag cagttgtttg ttgttaaaaa taacagccat tgtaatgaga cgcacaaact
     3961 aatatcacaa actggaaatg tctatcaata tatagttgct gatatcatgg agataattaa
     4021 aatgataacc atctcgcaaa taaataagta ttttactgtt ttcgtaacag ttttgtaata
     4081 aaaaaaccta taaatattcc ggattattca taccgtccca ccatcgggcg cggatctatg
     4141 ctactagtaa atcagtcaca ccaaggcttc aataaggaac acacaagcaa gatggtaagc
     4201 gctattgttt tatatgtgct tttggcggcg gcggcgcatt ctgcctttgc ggcggatccc
     4261 cagaagcagt attgggtctg caactcatcc gatgcaagta tttcatacac ctactgtgat
     4321 aaaatgcaat acccaatttc aattaatgtt aacccctgta tagaattgaa agggtccaaa
     4381 ggattattgc acattttcta cattccaagg agagatttaa agcaattata tttcaatctc
     4441 tatataactg tcaacaccat gaatcttcca aagcgcaaag aagttatttg ccgaggatct
     4501 gatgacgatt actctttttg cagagctctg aagggagaga ctgtgaatac aacaatatca
     4561 ttctccttca agggaataaa attttctaag ggaaaataca aatgtgttgt tgaagctatt
     4621 tctgggagcc cagaagaaat gctcttttgc ttggagtttg tcatcctaca ccaacctaat
     4681 tcaaattcta gactggttcc gcgtggttcc gctgcaaatg ctgcgcaaca cgatgaagcc
     4741 gtagacaaca aattcaacaa agaacaacaa aacgcgttct atgagatctt acatttacct
     4801 aacttaaacg aagaacaacg aaacgccttc atccaaagtt taaaagatga cccaagccaa
     4861 agcgctaacc ttttagcaga agctaaaaag ctaaatgatg ctcaggcgcc gaaagtagac
     4921 aacaaattca acaaagaaca acaaaacgcg ttctatgaga tcttacattt acctaactta
     4981 aacgaagaac aacgaaacgc cttcatccaa agtttaaaag atgacccaag ccaaagcgct
     5041 aaccttttag cagaagctaa aaagctaaat gatgctcagg cgccgaaagt agacgcgaat
     5101 tcgagctcgg taccctgagc ggccgcctgg ttccgcgtgg ttcgcatcat catcatcatc
     5161 actgaagatc tgatcctttc ctgggacccg gcaagaacca aaaactcact ctcttcaagg
     5221 aaatccgtaa tgttaaaccc gacacgatga agcttgtcgt tggatggaaa ggaaaagagt
     5281 tctacaggga aacttggacc cgcttcatgg aagacagctt ccccattgtt aacgaccaag
     5341 aagtgatgga tgttttcctt gttgtcaaca tgcgtcccac tagacccaac cgttgttaca
     5401 aattcctggc ccaacacgct ctgcgttgcg accccgacta tgtacctcat gacgtgatta
     5461 ggatcgtcga gccttcatgg gtgggcagca acaacgagta ccgcatcagc ctggctaaga
     5521 agggcggcgg ctgcccaata atgaaccttc actctgagta caccaactcg ttcgaacagt
     5581 tcatcgatcg tgtcatctgg gagaacttct acaagcccat cgtttacatc ggtaccgact
     5641 ctgctgaaga ggaggaaatt ctccttgaag tttccctggt gttcaaagta aaggagtttg
     5701 caccagacgc acctctgttc actggtccgg cgtattaaaa cacgatacat tgttattagt
     5761 acatttatta agcgctagat tctgtgcgtt gttgatttac agacaattgt tgtacgtatt
     5821 ttaataattc attaaattta taatctttag ggtggtatgt tagagcgaaa atcaaatgat
     5881 tttcagcgtc tttatatctg aatttaaata ttaaatcctc aatagatttg taaaataggt
     5941 ttcgattagt ttcaaacaag ggttgttttt ccgaaccgat ggctggacta tctaatggat
     6001 tttcgctcaa cgccacaaaa cttgccaaat cttgtagcag caatctagct ttgtcgatat
     6061 tcgtttgtgt tttgttttgt aataaaggtt cgacgtcgtt caaaatatta tgcgcttttg
     6121 tatttctttc atcactgtcg ttagtgtaca attgactcga cgtaaacacg ttaaataaag
     6181 cttggacata tttaacatcg ggcgtgttag ctttattagg ccgattatcg tcgtcgtccc
     6241 aaccctcgtc gttagaagtt gcttccgaag acgattttgc catagccaca cgacgcctat
     6301 taattgtgtc ggctaacacg tccgcgatca aatttgtagt tgagcttttt ggaattattt
     6361 ctgattgcgg gcgtttttgg gcgggtttca atctaactgt gcccgatttt aattcagaca
     6421 acacgttaga aagcgatggt gcaggcggtg gtaacatttc agacggcaaa tctactaatg
     6481 gcggcggtgg tggagctgat gataaatcta ccatcggtgg aggcgcaggc ggggctggcg
     6541 gcggaggcgg aggcggaggt ggtggcggtg atgcagacgg cggtttaggc tcaaatgtct
     6601 ctttaggcaa cacagtcggc acctcaacta ttgtactggt ttcgggcgcc gtttttggtt
     6661 tgaccggtct gagacgagtg cgattttttt cgtttctaat agcttccaac aattgttgtc
     6721 tgtcgtctaa aggtgcagcg ggttgaggtt ccgtcggcat tggtggagcg ggcggcaatt
     6781 cagacatcga tggtggtggt ggtggtggag gcgctggaat gttaggcacg ggagaaggtg
     6841 gtggcggcgg tgccgccggt ataatttgtt ctggtttagt ttgttcgcgc acgattgtgg
     6901 gcaccggcgc aggcgccgct ggctgcacaa cggaaggtcg tctgcttcga ggcagcgctt
     6961 ggggtggtgg caattcaata ttataattgg aatacaaatc gtaaaaatct gctataagca
     7021 ttgtaatttc gctatcgttt accgtgccga tatttaacaa ccgctcaatg taagcaattg
     7081 tattgtaaag agattgtctc aagctcgcac gccgataaca agccttttca tttttactac
     7141 agcattgtag tggcgagaca cttcgctgtc gtcgacgtac atgtatgctt tgttgtcaaa
     7201 aacgtcgttg gcaagcttta aaatatttaa aagaacatct ctgttcagca ccactgtgtt
     7261 gtcgtaaatg ttgtttttga taatttgcgc ttccgcagta tcgacacgtt caaaaaattg
     7321 atgcgcatca attttgttgt tcctattatt gaataaataa gattgtacag attcatatct
     7381 acgattcgtc atggccacca caaatgctac gctgcaaacg ctggtacaat tttacgaaaa
     7441 ctgcaaaaac gtcaaaactc ggtataaaat aatcaacggg cgctttggca aaatatctat
     7501 tttatcgcac aagcccacta gcaaattgta tttgcagaaa acaatttcgg cgcacaattt
     7561 taacgctgac gaaataaaag ttcaccagtt aatgagcgac cacccaaatt ttataaaaat
     7621 ctattttaat cacggttcca tcaacaacca agtgatcgtg atggactaca ttgactgtcc
     7681 cgatttattt gaaacactac aaattaaagg cgagctttcg taccaacttg ttagcaatat
     7741 tattagacag ctgtgtgaag cgctcaacga tttgcacaag cacaatttca tacacaacga
     7801 cataaaactc gaaaatgtct tatatttcga agcacttgat cgcgtgtatg tttgcgatta
     7861 cggattgtgc aaacacgaaa actcacttag cgtgcacgac ggcacgttgg agtattttag
     7921 tccggaaaaa attcgacacc acaactatgc acgttcgttt gactggtacg ccgtcggcgt
     7981 gttaacatac aagttgctaa ccggcggcca attcgtaatc atggtcatag ctgtttcctg
     8041 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     8101 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     8161 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     8221 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     8281 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     8341 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     8401 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     8461 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     8521 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     8581 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     8641 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     8701 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     8761 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     8821 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     8881 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     8941 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     9001 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     9061 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     9121 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     9181 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     9241 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     9301 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     9361 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     9421 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     9481 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     9541 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     9601 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     9661 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     9721 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     9781 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     9841 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     9901 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     9961 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
    10021 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
    10081 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
    10141 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
    10201 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg
    10261 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
    10321 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
    10381 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
    10441 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc
    10501 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
    10561 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
    10621 gttgtaaaac gacggccagt
//
