LOCUS       sequence-27522-e         733 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Zeiser E, Frokjaer-Jensen C, Jorgensen E, Ahringer J
  TITLE     MosSCI and gateway compatible plasmid toolkit for constitutive and 
            inducible expression of transgenes in the C. elegans germline.
  JOURNAL   PLoS One. 2011;6(5):e20082. Epub 2011 May 26.
  PUBMED    21637852
REFERENCE   2  (bases 1 to 733)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(5)"; pages: "e20082. Epub 2011 May 26"
COMMENT     Sequence Label: pJA257
FEATURES             Location/Qualifiers
     source          1..733
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      30..353
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    complement(358..453)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(490..509)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(491..509)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(514..530)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(514..530)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(632..651)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 acnggcagga cgactataca atgagataaa tgcaaaatcc tttcaagcat tcccttcttc
       61 tctatcactc ttctttcttt ttgtcaaaaa attctctcgc taatttattt gcttttttaa
      121 tgttattatt ttatgacttt ttatagtcac tgaaaagttt gcatctgagt gaagtgaatg
      181 ctatcaaaat gtgattctgt ctgatgtact ttcacaatct ctcttcaatt ccattttgaa
      241 gtgctttaaa cccgaaaggt tgagaaaaat gcgagcgctc aaatatttgt attgtgttcg
      301 ttgagtgacc caacaaaaag aggaaacttt attgtgccgc caagaaaaaa gtctcagcaa
      361 ctttattata caaagttggc attataaaaa agcattgctt atcaatttgt tgcaacgaac
      421 aggtcactat cagtcaaaat aaaatcatta tttggagctc catggtagcg ttaacgcggc
      481 cgcgatatcc cctatagtga gtcgtattac atggtcatag ctgtttcctg gcagctctgg
      541 cccgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgaaca
      601 ataaaactgt ctgcttacat aaacagtaat acaaggggtg ttatgagcca tattcaacgg
      661 gaaacgtcga ggccgcgatt aattccaaca tggatgctga ttatatgggt ataatgggct
      721 cgcgatatgt cgg
//
