LOCUS       sequence-27523-e         760 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Zeiser E, Frokjaer-Jensen C, Jorgensen E, Ahringer J
  TITLE     MosSCI and gateway compatible plasmid toolkit for constitutive and 
            inducible expression of transgenes in the C. elegans germline.
  JOURNAL   PLoS One. 2011;6(5):e20082. Epub 2011 May 26.
  PUBMED    21637852
REFERENCE   2  (bases 1 to 760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(5)"; pages: "e20082. Epub 2011 May 26"
COMMENT     Sequence Label: pJA258
FEATURES             Location/Qualifiers
     source          1..760
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    44..139
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      complement(144..467)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
ORIGIN
        1 gaccnngggg ancgcggccg cgnttacgct accatggagc tccaaataat gattttattt
       61 tgactgatag tgacctgttc gttgcaacaa attgataagc aatgcttttt tataatgcca
      121 actttgtata ataaagttgc tgagactttt ttcttggcgg cacaataaag tttcctcttt
      181 ttgttgggtc actcaacgaa cacaatacaa atatttgagc gctcgcattt ttctcaacct
      241 ttcgggttta aagcacttca aaatggaatt gaagagagat tgtgaaagta catcagacag
      301 aatcacattt tgatagcatt cacttcactc agatgcaaac ttttcagtga ctataaaaag
      361 tcataaaata ataacattaa aaaagcaaat aaattagcga gagaattttt tgacaaaaag
      421 aaagaagagt gatagagaag aagggaatgc ttgaaaggat tttgcattta tctcatttgt
      481 atagttcgtc catgccatgt gtaatcccag cagctgttac aaactcttta aggaccatgt
      541 ggtctctctt ttcgttggga tctttcgaaa gggcagattg gtaggacagg taatggttgt
      601 ctggtaaaag gacagggcca tcgccaattg gagtattttg ttgataatgg tctgctagtt
      661 gaacgcttcc atcttcaatg ttgtgtctaa ttttgagttc tgaaattnaa tcagatnagt
      721 tagtagtaaa tcatgttaac tacacttgat ccntctttgt
//
