LOCUS       sequence-275230-        6211 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  10xUAS expression vector with a vermilion marker and a phiC31 attB 
            sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6211)
  AUTHORS   Kondo S, Takahashi T, Yamagata N, Imanishi Y, Katow H, Hiramatsu S, 
            Lynn K, Abe A, Kumaraswamy A, Tanimoto H
  TITLE     Neurochemical Organization of the Drosophila Brain Visualized by 
            Endogenously Tagged Neurotransmitter Receptors.
  JOURNAL   Cell Rep. 2020 Jan 7;30(1):284-297.e5. doi: 
            10.1016/j.celrep.2019.12.018.
  PUBMED    31914394
REFERENCE   2  (bases 1 to 6211)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2019.12"; journalName: "Cell Rep"; date: 
            "2020-01-7- 7"; volume: "30"; issue: "1"; pages: "284-297.e5"
COMMENT     Sequence Label: pBFv-UAS3
FEATURES             Location/Qualifiers
     source          1..6211
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    17..111
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    143..237
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        266..504
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     451..472
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     primer_bind     complement(557..573)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(557..573)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(1154..1174)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1154..1172)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1193..1209)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1193..1209)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1206..1228)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1217..1233
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1241..1247,1248..1265,1266..1271))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1286..1307
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1424..1441)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1595..2183)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1675..1694)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2354..3145,3146..3214))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2977..2996
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3215..3319)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3345..3800)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3482..3503)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3694..3713
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3927..3949
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3941..3958
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3942..3958
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3968..3987
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3968..3986
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    4194..4263
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     CDS             complement(join(4463..4580,4657..4750,4842..5448,5503..5636,
                     5697..5857,5931..5956))
                     /codon_start=1
                     /product="tryptophan oxygenase"
                     /label=vermilion
                     /note="selectable marker, required to synthesize the brown 
                     eye pigment in Drosophila"
                     /translation="MSCPYAGNGNDHDDSAVPLTTEVGKIYGEYLMLDKLLDAQCMLSE
                     EDKRPVHDEHLFIITHQAYELWFKQIIFEFDSIRDMLDAEVIDETKTLEIVKRLNRVVL
                     ILKLLVDQVPILETMTPLDFMDFRKYLAPASGFQSLQFRLIENKLGVLTEQRVRYNQKY
                     SDVFSDEEARNSIRNSEKDPSLLELVQRWLERTPGLEESGFNFWAKFQESVDRFLEAQV
                     QSAMEEPVEKAKNYRLMDIEKRREVYRSIFDPAVHDALVRRGDRRFSHRALQGAIMITF
                     YRDEPRFSQPHQLLTLLMDIDSLITKWRYNHVIMVQRMIGSQQLGTGGSSGYQYLRSTL
                     SDRYKVFLDLFNLSTFLIPREAIPPLDETIRKKLINKSV"
ORIGIN
        1 accttaagct gcaggtcgga gtactgtcct ccgagcggag tactgtcctc cgagcggagt
       61 actgtcctcc gagcggagta ctgtcctccg agcggagtac tgtcctccga gcggagactc
      121 tagcgagcac gcgtctgcag gtcggagtac tgtcctccga gcggagtact gtcctccgag
      181 cggagtactg tcctccgagc ggagtactgt cctccgagcg gagtactgtc ctccgagcgg
      241 agactctagc gagccatatg gagcgccgga gtataaatag aggcgcttcg tctacggagc
      301 gacaattcaa ttcaaacaag caaagtgaac acgtcgctaa gcgaaagcta agcaaataaa
      361 caagcgcagc tgaacaagct aaacaatctg cagtaaagtg caagttaaag tgaatcaatt
      421 aaaagtaacc agcaaccaag taaatcaact gcaactactg aaatctgcca agaagtaatt
      481 attgaataca agaagagaac tctgaatagg gaattggggc gcgccgctag cagatctgaa
      541 ttcctgcagc ccgggggatc cactagttct agagcggccg cgcgtcacgc cacttcaacg
      601 ctcgatggga gcgtcattgg tgggcggggt aaccgtcgaa atcagtgttt acgcttccaa
      661 tcgcaacaaa aaattcactg caacactgaa aagcatacga aaacgatgaa gattgtacga
      721 gaaaccataa agtattttat ccacaaagac acgtatagca gaaaagccaa gttaactcgg
      781 cgataagttg tgtacacaag aataaaatcg gccagattca gtgttgtcag aaataagaaa
      841 accccactat gtttttcttt gccttttctt tctcccagcg atcattcatt tcgtggtgaa
      901 agaacggggt cattgcacgg agtttcgact gcgggaaagc agagctgccg ttcacttcgt
      961 ctataattag cgctttctat tttccccgat tcgggccgct gctgcgcttt tccgcctgct
     1021 gtttgtggca agtgtagcag caggctgtgc acgcagtgtg tagggataac agggtaatga
     1081 cgtcgcatgc acttggcttt ccaccgttgg tatcgattct ctgggacgat gagtcgagct
     1141 ccagcttttg ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc
     1201 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     1261 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     1321 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     1381 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     1441 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     1501 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     1561 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     1621 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     1681 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     1741 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     1801 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     1861 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     1921 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     1981 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     2041 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     2101 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     2161 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     2221 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     2281 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     2341 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     2401 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     2461 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     2521 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     2581 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     2641 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     2701 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     2761 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     2821 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     2881 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     2941 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     3001 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     3061 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     3121 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     3181 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     3241 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     3301 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctaaattg taagcgttaa
     3361 tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc
     3421 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt
     3481 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa
     3541 aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg
     3601 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg
     3661 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc
     3721 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa
     3781 tgcgccgcta cagggcgcgt cccattcgcc attcaggctg cgcaactgtt gggaagggcg
     3841 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
     3901 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga
     3961 gcgcgcgtaa tacgactcac tatagggcga attgggtacc tcgacatgcc cgccgtgacc
     4021 gtcgagaacc cgctgacgct gccccgcgta tccgcacccg ccgacgccgt cgcacgtccc
     4081 gtgctcaccg tgaccaccgc gcccagcggt ttcgagggcg agggcttccc ggtgcgccgc
     4141 gcgttcgccg ggatcaacta ccgccacctc gacccgttca tcatgatgga ccagatgggt
     4201 gaggtggagt acgcgcccgg ggagcccaag ggcacgccct ggcacccgca ccgcggcttc
     4261 gagaccgtga cctacatcgt cgacaagctt ggatttattt tgttatgtta tatgtattat
     4321 atgtcagaca taaagaaaag gaacacatca aatgtgataa caaagactaa acaagtaatt
     4381 ttattacacc aaaacgacaa aacagtaggc agaacaaaca acgcatagcc aaacattgac
     4441 gaattggata ccctgccgat tgtcagacac ttttgttgat cagtttcttg cgaatggtct
     4501 cgtccagcgg tggaatcgcc tcgcggggaa tcagaaaagt ggacagattg aacagatcca
     4561 gaaacacctt gtaccgatca ctgaaaccaa aaaaaaacaa agggagaaca gtttgagttc
     4621 attgatcccc gatataatca catctgcgat gatcacctga gagtggagcg cagatattga
     4681 tatccagacg agccaccagt gcccaactgt tgggatccaa tcatgcgttg caccatgatc
     4741 acgtgattgt ctgcggcggg aatagaaagt atttggttag gaaaaccagt cttaaacata
     4801 agatatattt ataaaagagt atcaaagaat gcaatactta catctccact tggttattaa
     4861 cgagtcgatg tccatgagca gggtgagcaa ctggtgtggt tggctgaacc tgggttcatc
     4921 cctatagaag gtgatcatga tggctccctg aagggcacga tggctaaacc ggcgatcccc
     4981 acgacgcacc agtgcatcgt gcactgccgg atcaaagatg gagcgataca cctcgcgtcg
     5041 cttctcaatg tccatgaggc ggtagttttt cgccttctcc acgggctcct ccatggcgct
     5101 ctgtacctgc gcctccagga atcgatcgac gctctcctga aacttggccc agaagttgaa
     5161 gccactctcc tccagtccgg gcgtcctctc cagccatcgc tgcactagct ccagtagcga
     5221 gggatctttc tccgagttgc gaatcgagtt ccgcgcctcc tcgtcgctaa agacatccga
     5281 gtacttctgg ttgtatctca cccgctgctc tgtcagaact cccagcttgt tctcgatcaa
     5341 acggaactgc agcgactgaa aaccagatgc gggtgccagg tacttgcgga agtccatgaa
     5401 gtctagcggg gtcatggtct ccagaatggg cacttggtcc accaggagct gtacaaagga
     5461 agttataaac ggattttggt aagagattca gaaagcactc acttttagaa tcagaaccac
     5521 tcggttcagt cgcttgacaa tctccagcgt cttggtttca tcgatgacct ctgcatccaa
     5581 catgtctcgt atggagtcga actcaaagat gatctgcttg aaccaaagct cgtaggctgt
     5641 ggcgaaggta cttaaatgcc attgagtgtt gtcatcaaag ttgtaaacct actcaccctg
     5701 gtgcgtgatg atgaacagat gctcatcgtg cacgggtcgc ttgtcctcct cggacagcat
     5761 acactgggca tccagcagtt tgtccagcat cagatactct ccatagattt tgcccacttc
     5821 cgtggttaat ggcaccgccg aatcatcgtg atcgtttctg tatgggtttg aattgaatcg
     5881 cagaactgaa gatcgattgg cattcctgga cagcacgtgc tggtgctcac ccgtttcctg
     5941 catagggaca gctcatggtg cacagctcag atcagatcgt gactcctcga gcggcggatg
     6001 ctggcgaact gatctccgcc agcggaccgg agatgagacc ccagcgaacc gataacagag
     6061 cgagagagct ccagttccga ctgattgcac agtcggtgat ctgggcgatg ggcactgcca
     6121 gataggctgg gaattatcaa tcacttgagg tgaaagtgcg gcgcacacaa atccaagctt
     6181 gatatcgaat tccctgcagg gccaaccaac a
//
