LOCUS       sequence-27524-e         720 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 720)
  AUTHORS   Zeiser E, Frokjaer-Jensen C, Jorgensen E, Ahringer J
  TITLE     MosSCI and gateway compatible plasmid toolkit for constitutive and 
            inducible expression of transgenes in the C. elegans germline.
  JOURNAL   PLoS One. 2011;6(5):e20082. Epub 2011 May 26.
  PUBMED    21637852
REFERENCE   2  (bases 1 to 720)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(5)"; pages: "e20082. Epub 2011 May 26"
COMMENT     Sequence Label: pJA258
FEATURES             Location/Qualifiers
     source          1..720
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(51..174)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(225..253)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 ctctagagct cgaattctac aggtcactaa taccatctaa gtagttggtt catagtgact
       61 gcatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc taatttaata
      121 tattgatatt tatatcattt tacgtttctc gttcaacttt cttgtacaaa gtggagggag
      181 gtggaggtgg agctatgagt aaaggagaag aacttttcac tggagttgtc ccaattcttg
      241 ttgaattaga tggtgatgtt aatgggcaca aattttctgt cagtggagag ggtgaaggtg
      301 atgcaacata cggaaaactt acccttaaat ttatttgcac tactggaaaa ctacctgttc
      361 catgggtaag tttaaacata tatatactaa ctaaccctga ttatttaaat tttcagccaa
      421 cacttgtcac tactttcggt tatggtctta tgtgcttcgc cagataccca gatcatatga
      481 aacagcatga ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa agaactatat
      541 ttttcaaaga tgacgggaac tacaagacac gtaagtttaa acagttcggt actaactaac
      601 catacatatt taaattttca ggtgctgaag tcaagtttga aggtgatacc cttgttaata
      661 gaatcgagtt aaaaggtatt gattttaaag aagatggaaa cattcttgga cacaaattgg
//
