LOCUS       sequence-275248-       12542 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PgadE - TARGET3 - MevT - MBIS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12542)
  AUTHORS   Glasscock CJ, Biggs BW, Lazar JT, Arnold JH, Burdette LA, Valdes A, 
            Kang MK, Tullman-Ercek D, Tyo KEJ, Lucks JB
  TITLE     Dynamic Control of Gene Expression with Riboregulated Switchable 
            Feedback Promoters.
  JOURNAL   ACS Synth Biol. 2021 May 21;10(5):1199-1213. doi: 
            10.1021/acssynbio.1c00015. Epub 2021 Apr 9.
  PUBMED    33834762
REFERENCE   2  (bases 1 to 12542)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00015"; journalName: "ACS Synth Biol"; date: 
            "2021-05-21- 21"; volume: "10"; issue: "5"; pages: "1199-1213"
COMMENT     Sequence Label: pJBL6729
FEATURES             Location/Qualifiers
     source          1..12542
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1600..1611)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             4524..4535
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             8142..8153
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      10567..10638
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      10654..10681
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      10720..10791
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      10807..10834
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(10862..10879)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10996..11541)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(11076..11095)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(11655..11749)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(11770..12429)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     12344..12363
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(12430..12532)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
ORIGIN
        1 tgaattcacg agatctgcgg atgggcagta ttggcgctga acgcctgctg gcgcgtattc
       61 gtggcgttaa tactctctcc gctacgcagt gttgtagatc aattgcgcac tatcattgaa
      121 ataattacct gctagtgatt atttcaacct actgaatttc atctaatttt tttcactcta
      181 tggcaaatta gccatttcaa acattatcat ggctgatatt ttccgtagtc aggtttaatg
      241 ttttaaaagt gctgtgggaa agtgaacaaa gagttccgta agcgttgatg ctatgggcgg
      301 ttaaataagt aatccgggtt catttttttg caactggcgt tgattacatt gcataaatat
      361 ccgtgtctcc agacgctata taaaaacctg aagacatgaa tgcgttattt actcaggtaa
      421 tttcaatgcg ttaaaagaaa gctggcaatc caattgccag cttaagtcga aacaaggaga
      481 ctcgatattt aaatcggatt acattttaac tttagtaata ttcttcagag atcacaaact
      541 ggttattgat aacttattct tgggcagtaa tccgcaaacg ttaacttttt gtttgctatt
      601 tacaagctga taacaaccag gaatcttact taggatcaat atatggagtg cgtgatggat
      661 aaatctgaag tattgattag tgttaataga cgtattagtt cacgaagggt aaagttctta
      721 taggcgttta ctatattgaa caacgattcg gacaaggatg taaataatga aaaggatgac
      781 atattcgaaa cgacctctat cctatctatc tgcctgtcct gtcctgtgtc tttgcgggga
      841 atgtatacag ttcatgtata tattccccgc tttttttttg gatcttagga ggaatataaa
      901 atgaaaaatt gtgtcatcgt cagtgcggta cgtactgcta tcggtagttt taacggttca
      961 ctcgcttcca ccagcgccat cgacctgggg gcgacagtaa ttaaagccgc cattgaacgt
     1021 gcaaaaatcg attcacaaca cgttgatgaa gtgattatgg gtaacgtgtt acaagccggg
     1081 ctggggcaaa atccggcgcg tcaggcactg ttaaaaagcg ggctggcaga aacggtgtgc
     1141 ggattcacgg tcaataaagt atgtggttcg ggtcttaaaa gtgtggcgct tgccgcccag
     1201 gccattcagg caggtcaggc gcagagcatt gtggcggggg gtatggaaaa tatgagttta
     1261 gccccctact tactcgatgc aaaagcacgc tctggttatc gtcttggaga cggacaggtt
     1321 tatgacgtaa tcctgcgcga tggcctgatg tgcgccaccc atggttatca tatggggatt
     1381 accgccgaaa acgtggctaa agagtacgga attacccgtg aaatgcagga tgaactggcg
     1441 ctacattcac agcgtaaagc ggcagccgca attgagtccg gtgcttttac agccgaaatc
     1501 gtcccggtaa atgttgtcac tcgaaagaaa accttcgtct tcagtcaaga cgagttcccg
     1561 aaagcgaact caacggctga agcgttaggt gcattgcgcc cggccttcga taaagcagga
     1621 acagtcaccg ctgggaacgc gtctggtatt aacgacggtg ctgccgctct ggtgattatg
     1681 gaagaatctg cggcgctggc agcaggcctt acccccctgg ctcgcattaa aagttatgcc
     1741 agcggtggcg tgccccccgc attgatgggt atggggccag tacctgccac gcaaaaagcg
     1801 ttacaactgg cggggctgca actggcggat attgatctca ttgaggctaa tgaagcattt
     1861 gctgcacagt tccttgccgt tgggaaaaac ctgggctttg attctgagaa agtgaatgtc
     1921 aacggcgggg ccatcgcgct cgggcatcct atcggtgcca gtggtgctcg tattctggtc
     1981 acactattac atgccatgca ggcacgcgat aaaacgctgg ggctggcaac actgtgcatt
     2041 ggcggcggtc agggaattgc gatggtgatt gaacggttga attgaggatc ttgaattaag
     2101 gaggacagct aaatgaaact ctcaactaaa ctttgttggt gtggtattaa aggaagactt
     2161 aggccgcaaa agcaacaaca attacacaat acaaacttgc aaatgactga actaaaaaaa
     2221 caaaagaccg ctgaacaaaa aaccagacct caaaatgtcg gtattaaagg tatccaaatt
     2281 tacatcccaa ctcaatgtgt caaccaatct gagctagaga aatttgatgg cgtttctcaa
     2341 ggtaaataca caattggtct gggccaaacc aacatgtctt ttgtcaatga cagagaagat
     2401 atctactcga tgtccctaac tgttttgtct aagttgatca agagttacaa catcgacacc
     2461 aacaaaattg gtagattaga agtcggtact gaaactctga ttgacaagtc caagtctgtc
     2521 aagtctgtct tgatgcaatt gtttggtgaa aacactgacg tcgaaggtat tgacacgctt
     2581 aatgcctgtt acggtggtac caacgcgttg ttcaactctt tgaactggat tgaatctaac
     2641 gcatgggatg gtagagacgc cattgtagtt tgcggtgata ttgccatcta cgataagggt
     2701 gccgcaagac caaccggtgg tgccggtact gttgctatgt ggatcggtcc tgatgctcca
     2761 attgtatttg actctgtaag agcttcttac atggaacacg cctacgattt ttacaagcca
     2821 gatttcacca gcgaatatcc ttacgtcgat ggtcattttt cattaacttg ttacgtcaag
     2881 gctcttgatc aagtttacaa gagttattcc aagaaggcta tttctaaagg gttggttagc
     2941 gatcccgctg gttcggatgc tttgaacgtt ttgaaatatt tcgactacaa cgttttccat
     3001 gttccaacct gtaaattggt cacaaaatca tacggtagat tactatataa cgatttcaga
     3061 gccaatcctc aattgttccc agaagttgac gccgaattag ctactcgcga ttatgacgaa
     3121 tctttaaccg ataagaacat tgaaaaaact tttgttaatg ttgctaagcc attccacaaa
     3181 gagagagttg cccaatcttt gattgttcca acaaacacag gtaacatgta caccgcatct
     3241 gtttatgccg cctttgcatc tctattaaac tatgttggat ctgacgactt acaaggcaag
     3301 cgtgttggtt tattttctta cggttccggt ttagctgcat ctctatattc ttgcaaaatt
     3361 gttggtgacg tccaacatat tatcaaggaa ttagatatta ctaacaaatt agccaagaga
     3421 atcaccgaaa ctccaaagga ttacgaagct gccatcgaat tgagagaaaa tgcccatttg
     3481 aagaagaact tcaaacctca aggttccatt gagcatttgc aaagtggtgt ttactacttg
     3541 accaacatcg atgacaaatt tagaagatcc tacgatgtta aaaaatgagg atctaaaata
     3601 aggaggatta cactatggtt ttaaccaata aaacagtcat ttctggatcg aaagtcaaaa
     3661 gtttatcatc tgcgcaatcg agctcatcag gaccttcatc atctagtgag gaagatgatt
     3721 cccgcgatat tgaaagcttg gataagaaaa tacgtccttt agaagaatta gaagcattat
     3781 taagtagtgg aaatacaaaa caattgaaga acaaagaggt cgctgccttg gttattcacg
     3841 gtaagttacc tttgtacgct ttggagaaaa aattaggtga tactacgaga gcggttgcgg
     3901 tacgtaggaa ggctctttca attttggcag aagctcctgt attagcatct gatcgtttac
     3961 catataaaaa ttatgactac gaccgcgtat ttggcgcttg ttgtgaaaat gttataggtt
     4021 acatgccttt gcccgttggt gttataggcc ccttggttat cgatggtaca tcttatcata
     4081 taccaatggc aactacagag ggttgtttgg tagcttctgc catgcgtggc tgtaaggcaa
     4141 tcaatgctgg cggtggtgca acaactgttt taactaagga tggtatgaca agaggcccag
     4201 tagtccgttt cccaactttg aaaagatccg gtgcctgtaa gatatggtta gactcagaag
     4261 agggacaaaa cgcaattaaa aaagctttta actctacatc aagatttgca cgtctgcaac
     4321 atattcaaac ttgtctagca ggagatttac tcttcatgag atttagaaca actactggtg
     4381 acgcaatggg tatgaatatg atttctaaag gtgtcgaata ctcattaaag caaatggtag
     4441 aagagtatgg ctgggaagat atggaggttg tctccgtttc tggtaactac tgtaccgaca
     4501 aaaaaccagc tgccatcaac tggatcgaag gtcgtggtaa gagtgtcgtc gcagaagcta
     4561 ctattcctgg tgatgttgtc agaaaagtgt taaaaagtga tgtttccgca ttggttgagt
     4621 tgaacattgc taagaatttg gttggatctg caatggctgg gtctgttggt ggatttaacg
     4681 cacatgcagc taatttagtg acagctgttt tcttggcatt aggacaagat cctgcacaaa
     4741 atgttgaaag ttccaactgt ataacattga tgaaagaagt ggacggtgat ttgagaattt
     4801 ccgtatccat gccatccatc gaagtaggta ccatcggtgg tggtactgtt ctagaaccac
     4861 aaggtgccat gttggactta ttaggtgtaa gaggcccgca tgctaccgct cctggtacca
     4921 acgcacgtca attagcaaga atagttgcct gtgccgtctt ggcaggtgaa ttatccttat
     4981 gtgctgccct agcagccggc catttggttc aaagtcatat gacccacaac aggaaacctg
     5041 ctgaaccaac aaaacctaac aatttggacg ccactgatat aaatcgtttg aaagatgggt
     5101 ccgtcacctg cattaaatcc tgaggatcta ggaggttaat tggatgtcat taccgttctt
     5161 aacttctgca ccgggaaagg ttattatttt tggtgaacac tctgctgtgt acaacaagcc
     5221 tgccgtcgct gctagtgtgt ctgcgttgag aacctacctg ctaataagcg agtcatctgc
     5281 accagatact attgaattgg acttcccgga cattagcttt aatcataagt ggtccatcaa
     5341 tgatttcaat gccatcaccg aggatcaagt aaactcccaa aaattggcca aggctcaaca
     5401 agccaccgat ggcttgtctc aggaactcgt tagtcttttg gacccgttgt tagctcaact
     5461 atccgaatcc ttccactacc atgcagcgtt ttgtttcctg tatatgtttg tttgcctatg
     5521 cccccatgcc aagaatatta agttttcttt aaagtctact ttacccatcg gtgctgggtt
     5581 gggctcaagc gcctctattt ctgtatcact ggccttagct atggcctact tgggggggtt
     5641 aataggatct aatgacttgg aaaagctgtc agaaaacgat aagcatatag tgaatcaatg
     5701 ggccttcata ggtgaaaagt gtattcacgg taccccttca ggaatagata acgctgtggc
     5761 cacttatggt aatgccctgc tatttgaaaa agactcacat aatggaacaa taaacacaaa
     5821 caattttaag ttcttagatg atttcccagc cattccaatg atcctaacct atactaggat
     5881 tccaaggtct acaaaagacc ttgttgctcg cgttcgtgtg ttggtcaccg agaaatttcc
     5941 tgaagttatg aagccaattc tagatgccat gggtgaatgt gccctacaag gcttagagat
     6001 catgactaag ttaagtaaat gtaaaggcac cgatgacgag gctgtagaaa ataataatga
     6061 actgtatgaa caactattgg aattgataag aataaatcat ggactgcttg tctcaatcgg
     6121 tgtttctcat cctggattag aacttattaa aaatctgagc gatgatttga gaattggctc
     6181 cacaaaactt accggtgctg gtggcggcgg ttgctctttg actttgttac gaagagacat
     6241 tactcaagag caaattgaca gcttcaaaaa gaaattgcaa gatgatttta gttacgagac
     6301 atttgaaaca gacttgggtg ggactggctg ctgtttgtta agcgcaaaaa atttgaataa
     6361 agaccttaaa atcaaatccc tagtattcca attatttgaa aataaaacta ccacaaagca
     6421 acaaattgac gatctattat tgccaggaaa cacgaattta ccatggactt catagggatc
     6481 ttaaggagga taccctatgt cagagttgag agccttcagt gccccaggga aagcgttact
     6541 agctggtgga tatttagttt tagatacaaa atatgaagca tttgtagtcg gattatcggc
     6601 aagaatgcat gctgtagccc atccttacgg ttcattgcaa gggtctgata agtttgaagt
     6661 gcgtgtgaaa agtaaacaat ttaaagatgg ggagtggctg taccatataa gtcctaaaag
     6721 tggcttcatt cctgtttcga taggcggatc taagaaccct ttcattgaaa aagttatcgc
     6781 taacgtattt agctacttta aacctaacat ggacgactac tgcaatagaa acttgttcgt
     6841 tattgatatt ttctctgatg atgcctacca ttctcaggag gatagcgtta ccgaacatcg
     6901 tggcaacaga agattgagtt ttcattcgca cagaattgaa gaagttccca aaacagggct
     6961 gggctcctcg gcaggtttag tcacagtttt aactacagct ttggcctcct tttttgtatc
     7021 ggacctggaa aataatgtag acaaatatag agaagttatt cataatttag cacaagttgc
     7081 tcattgtcaa gctcagggta aaattggaag cgggtttgat gtagcggcgg cagcatatgg
     7141 atctatcaga tatagaagat tcccacccgc attaatctct aatttgccag atattggaag
     7201 tgctacttac ggcagtaaac tggcgcattt ggttgatgaa gaagactgga atattacgat
     7261 taaaagtaac catttacctt cgggattaac tttatggatg ggcgatatta agaatggttc
     7321 agaaacagta aaactggtcc agaaggtaaa aaattggtat gattcgcata tgccagaaag
     7381 cttgaaaata tatacagaac tcgatcatgc aaattctaga tttatggatg gactatctaa
     7441 actagatcgc ttacacgaga ctcatgacga ttacagcgat cagatatttg agtctcttga
     7501 gaggaatgac tgtacctgtc aaaagtatcc tgaaatcaca gaagttagag atgcagttgc
     7561 cacaattaga cgttccttta gaaaaataac taaagaatct ggtgccgata tcgaacctcc
     7621 cgtacaaact agcttattgg atgattgcca gaccttaaaa ggagttctta cttgcttaat
     7681 acctggtgct ggtggttatg acgccattgc agtgattact aagcaagatg ttgatcttag
     7741 ggctcaaacc gctaatgaca aaagattttc taaggttcaa tggctggatg taactcaggc
     7801 tgactggggt gttaggaaag aaaaagatcc ggaaacttat cttgataaat agggatctag
     7861 gaggattatg agatgaccgt ttacacagca tccgttaccg cacccgtcaa catcgcaacc
     7921 cttaagtatt gggggaaaag ggacacgaag ttgaatctgc ccaccaattc gtccatatca
     7981 gtgactttat cgcaagatga cctcagaacg ttgacctctg cggctactgc acctgagttt
     8041 gaacgcgaca ctttgtggtt aaatggagaa ccacacagca tcgacaatga aagaactcaa
     8101 aattgtctgc gcgacctacg ccaattaaga aaggaaatgg aatcgaagga cgcctcattg
     8161 cccacattat ctcaatggaa actccacatt gtctccgaaa ataactttcc tacagcagct
     8221 ggtttagctt cctccgctgc tggctttgct gcattggtct ctgcaattgc taagttatac
     8281 caattaccac agtcaacttc agaaatatct agaatagcaa gaaaggggtc tggttcagct
     8341 tgtagatcgt tgtttggcgg atacgtggcc tgggaaatgg gaaaagctga agatggtcat
     8401 gattccatgg cagtacaaat cgcagacagc tctgactggc ctcagatgaa agcttgtgtc
     8461 ctagttgtca gcgatattaa aaaggatgtg agttccactc agggtatgca attgaccgtg
     8521 gcaacctccg aactatttaa agaaagaatt gaacatgtcg taccaaagag atttgaagtc
     8581 atgcgtaaag ccattgttga aaaagatttc gccacctttg caaaggaaac aatgatggat
     8641 tccaactctt tccatgccac atgtttggac tctttccctc caatattcta catgaatgac
     8701 acttccaagc gtatcatcag ttggtgccac accattaatc agttttacgg agaaacaatc
     8761 gttgcataca cgtttgatgc aggtccaaat gctgtgttgt actacttagc tgaaaatgag
     8821 tcgaaactct ttgcatttat ctataaattg tttggctctg ttcctggatg ggacaagaaa
     8881 tttactactg agcagcttga ggctttcaac catcaatttg aatcatctaa ctttactgca
     8941 cgtgaattgg atcttgagtt gcaaaaggat gttgccagag tgattttaac tcaagtcggt
     9001 tcaggcccac aagaaacaaa cgaatctttg attgacgcaa agactggtct accaaaggaa
     9061 taaggatcta ggaggtaatg ataatgcaaa cggaacacgt cattttattg aatgcacagg
     9121 gagttcccac gggtacgctg gaaaagtatg ccgcacacac ggcagacacc cgcttacatc
     9181 tcgcgttctc cagttggctg tttaatgcca aaggacaatt attagttacc cgccgcgcac
     9241 tgagcaaaaa agcatggcct ggcgtgtgga ctaactcggt ttgtgggcac ccacaactgg
     9301 gagaaagcaa cgaagacgca gtgatccgcc gttgccgtta tgagcttggc gtggaaatta
     9361 cgcctcctga atctatctat cctgactttc gctaccgcgc caccgatccg agtggcattg
     9421 tggaaaatga agtgtgtccg gtatttgccg cacgcaccac tagtgcgtta cagatcaatg
     9481 atgatgaagt gatggattat caatggtgtg atttagcaga tgtattacac ggtattgatg
     9541 ccacgccgtg ggcgttcagt ccgtggatgg tgatgcaggc gacaaatcgc gaagccagaa
     9601 aacgattatc tgcatttacc cagcttaaat aaggatctag gaggttactc atatggactt
     9661 tccgcagcaa ctcgaagcct gcgttaagca ggccaaccag gcgctgagcc gttttatcgc
     9721 cccactgccc tttcagaaca ctcccgtggt cgaaaccatg cagtatggcg cattattagg
     9781 tggtaagcgc ctgcgacctt tcctggttta tgccaccggt catatgttcg gcgttagcac
     9841 aaacacgctg gacgcacccg ctgccgccgt tgagtgtatc cacgcttact cattaattca
     9901 tgatgattta ccggcaatgg atgatgacga tctgcgtcgc ggtttgccaa cctgccatgt
     9961 gaagtttggc gaagcaaacg cgattctcgc tggcgacgct ttacaaacgc tggcgttctc
    10021 gattttaagc gatgccgata tgccggaagt gtcggaccgc gacagaattt cgatgatttc
    10081 tgaactggcg agcgccagtg gtattgccgg aatgtgcggt ggtcaggcat tagatttaga
    10141 cgcggaaggc aaacacgtac ctctggacgc gcttgagcgt attcatcgtc ataaaaccgg
    10201 cgcattgatt cgcgccgccg ttcgccttgg tgcattaagc gccggagata aaggacgtcg
    10261 tgctctgccg gtactcgaca agtatgcaga gagcatcggc cttgccttcc aggttcagga
    10321 tgacatcctg gatgtggtgg gagatactgc aacgttggga aaacgccagg gtgccgacca
    10381 gcaacttggt aaaagtacct accctgcact tctgggcctt gagcaagccc ggaagaaagc
    10441 ccgggatctg atcgacgatg cccgtcagtc gctgaaacaa ctggctgaac agtcactcga
    10501 tacctcggca ctggaagcgc tagcggacta catcatccag cgtaataaat aaggatctcc
    10561 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
    10621 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc
    10681 gtttatagga tcctaactcg agtaaggatc tccaggcatc aaataaaacg aaaggctcag
    10741 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt
    10801 cacactggct caccttcggg tgggcctttc tgcgtttata cctagggata tattccgctt
    10861 cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg gcgagcggaa atggcttacg
    10921 aacggggcgg agatttcctg gaagatgcca ggaagatact taacagggaa gtgagagggc
    10981 cgcggcaaag ccgtttttcc ataggctccg cccccctgac aagcatcacg aaatctgacg
    11041 ctcaaatcag tggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
    11101 cggctccctc gtgcgctctc ctgttcctgc ctttcggttt accggtgtca ttccgctgtt
    11161 atggccgcgt ttgtctcatt ccacgcctga cactcagttc cgggtaggca gttcgctcca
    11221 agctggactg tatgcacgaa ccccccgttc agtccgaccg ctgcgcctta tccggtaact
    11281 atcgtcttga gtccaacccg gaaagacatg caaaagcacc actggcagca gccactggta
    11341 attgatttag aggagttagt cttgaagtca tgcgccggtt aaggctaaac tgaaaggaca
    11401 agttttggtg actgcgctcc tccaagccag ttacctcggt tcaaagagtt ggtagctcag
    11461 agaaccttcg aaaaaccgcc ctgcaaggcg gttttttcgt tttcagagca agagattacg
    11521 cgcagaccaa aacgatctca agaagatcat cttattaatc agataaaata tttctagatt
    11581 tcagtgcaat ttatctcttc aaatgtagca cctgaagtca gccccatacg atataagttg
    11641 ttactagtgc ttggattctc accaataaaa aacgcccggc ggcaaccgag cgttctgaac
    11701 aaatccagat ggagttctga ggtcattact ggatctatca acaggagtcc aagcgagctc
    11761 gatatcaaat tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat
    11821 tctgccgaca tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag
    11881 caccttgtcg ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc
    11941 catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg ctgagacgaa
    12001 aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac
    12061 atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagcga
    12121 tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat
    12181 caccagctca ccgtctttca ttgccatacg aaattccgga tgagcattca tcaggcgggc
    12241 aagaatgtga ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa
    12301 ggccgtaata tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc
    12361 ctcaaaatgt tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt
    12421 tttctccatt ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg
    12481 tagtgatctt atttcattat ggtgaaagtt ggaacctctt acgtgccgat caacgtctca
    12541 tt
//
