LOCUS       sequence-27525-e         754 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Zeiser E, Frokjaer-Jensen C, Jorgensen E, Ahringer J
  TITLE     MosSCI and gateway compatible plasmid toolkit for constitutive and 
            inducible expression of transgenes in the C. elegans germline.
  JOURNAL   PLoS One. 2011;6(5):e20082. Epub 2011 May 26.
  PUBMED    21637852
REFERENCE   2  (bases 1 to 754)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(5)"; pages: "e20082. Epub 2011 May 26"
COMMENT     Sequence Label: pJA252
FEATURES             Location/Qualifiers
     source          1..754
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(67..190)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(680..700)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 tcgcggccgc ctgcagctct agagctcgaa ttctacaggt cactaatacc atctaagtag
       61 ttggttcata gtgactgcat atgttgtgtt ttacagtatt atgtagtctg ttttttatgc
      121 aaaatctaat ttaatatatt gatatttata tcattttacg tttctcgttc aacttttttg
      181 tacaaacttg ctctctgtct gaaacattca attgattatc gataaaatta attaagagag
      241 aatacaatga gaaaacggaa aatcgagcag gattttgggg aaagtcgggg aatttttgct
      301 atttaagctt tctttaacaa agaaactgca tttttccatg agattaccat gaaatttgcg
      361 ttaaatgggt gaatttataa catttacggc tagaaattaa ttttaaaaaa gagaataaac
      421 gaaaaattat tcgagaaaac gtctttgaag cagaaatgaa atgaaaaaac aaccgccagc
      481 gctgacaaaa atgatcaaac aaacggagta cggtatacgc ggtaagaccc aaatgaattt
      541 taattcggtg cgcctttaaa aaatgtattt ttttaatttc tttatccgtg ggcccaaatg
      601 tatttttttc ttgtggtttg atgtaaaaat tgggttatat tatttgtttt atggtttaat
      661 tttttaaaaa ctgatatttc aacttttcta tacaaagttg gcattataaa aaagcattgc
      721 ttatcaattt gttgcaacga caggtcacta tcag
//
