LOCUS       sequence-27526-e         750 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Zeiser E, Frokjaer-Jensen C, Jorgensen E, Ahringer J
  TITLE     MosSCI and gateway compatible plasmid toolkit for constitutive and 
            inducible expression of transgenes in the C. elegans germline.
  JOURNAL   PLoS One. 2011;6(5):e20082. Epub 2011 May 26.
  PUBMED    21637852
REFERENCE   2  (bases 1 to 750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(5)"; pages: "e20082. Epub 2011 May 26"
COMMENT     Sequence Label: pJA269
FEATURES             Location/Qualifiers
     source          1..750
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    18..113
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(542..570)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 cgctaccatg gagctccaaa taatgatttt attttgactg atagtgacct gttcgttgca
       61 acaaattgat aagcaatgct tttttataat gccaactttg tatagaaaag ttggatcacc
      121 aaaaacggaa cgttgagctg gacggaaata gtggtaaagt gacatgatta tagtttgaag
      181 atttctaatt tcacaattag agcaaatgtt gttcggtatt tattttcaac ggtatttata
      241 ctattttcca cctttttcta gaacattcga gctgcttgtt gcaaaaggag ggcgactcac
      301 attcggtaca tggaaaagta gtgtacacaa taaagagacc cagatacatt ttccgtctgc
      361 gtctctttgc acccaccggg agtattttca aacgaatgca tctaggacct tctagaacat
      421 tctgtaaggc tgcagaatgc gggtatataa ggaaagcggg ctcagaggaa gccaacacgc
      481 tttgttctag tgcatctaaa aaacttcgaa aatgagtaaa ggagaagaac ttttcactgg
      541 agttgtccca attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag
      601 tggagagggt gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac
      661 tggaaaacta cctgttccat gggtaagttt aaacatatat atactaacta accctgatta
      721 tttaaatttt cagccaacac ttgtcactac
//
