LOCUS       sequence-275262-       12750 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  L1 in position 4, for AtcoCas9 (Puchta) expression in L2 CRISPR 
            vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12750)
  AUTHORS   Sauret-Gueto S, Frangedakis E, Silvestri L, Rebmann M, Tomaselli M, 
            Markel K, Delmans M, West A, Patron NJ, Haseloff J
  TITLE     Systematic tools for reprogramming plant gene expression in a simple
            model, Marchantia polymorpha.
  JOURNAL   ACS Synth Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511.
  PUBMED    32163700
REFERENCE   2  (bases 1 to 12750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511."
COMMENT     Sequence Label: L1_Cas9-Ck4
FEATURES             Location/Qualifiers
     source          1..12750
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(257..1072)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(257..1072)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     980..999
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     misc_feature    1505..1529
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             3291..7394
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             3291..7394
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             7407..7427
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      7451..7703
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     polyA_signal    7750..7924
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    7945..7969
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             9270..9899
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             10328..11401
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      11467..11661
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(11897..11919)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    12005..12145
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     12060..12079
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(12160..12177)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(12331..12750,1..169))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12411..12430)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
       61 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      121 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
      181 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
      241 catgcattct aggtgattag aaaaactcat cgagcatcaa atgaaactgc aatttattca
      301 tatcaggatt atcaatacca tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact
      361 caccgaggca gttccatagg atggcaagat cctggtatcg gtctgcgatt ccgactcgtc
      421 caacatcaat acaacctatt aatttcccct cgtcaaaaat aaggttatca agtgagaaat
      481 caccatgagt gacgactgaa tccggtgaga atggcaaaag tttatgcatt tctttccaga
      541 cttgttcaac aggccagcca ttacgctcgt catcaaaatc actcgcatca accaaaccgt
      601 tattcattcg tgattgcgcc tgagcgagtc gaaatacgcg atcgctgtta aaaggacaat
      661 tacaaacagg aatcgaatgc aaccggcgca ggaacactgc cagcgcatca acaatatttt
      721 cacctgaatc aggatattct tctaatacct ggaatgctgt tttccctggg atcgcagtgg
      781 tgagtaacca tgcatcatca ggagtacgga taaaatgctt gatggtcgga agaggcataa
      841 attccgtcag ccagtttagt ctgaccatct catctgtaac atcattggca acgctacctt
      901 tgccatgttt cagaaacaac tctggcgcat cgggcttccc atacaatcgg tagattgtcg
      961 cacctgattg cccgacatta tcgcgagccc atttataccc atataaatca gcatccatgt
     1021 tggaatttaa tcgcggcctt gagcaagacg tttcccgttg aatatggctc ataacagaac
     1081 ttattatttc cttcctcttt tctacagtat ttaaagatac cccaagaagc taattataac
     1141 aagacgaact ccaattcact gttccttgca ttctaaaacc ttaaatacca gaaaacagct
     1201 ttttcaaagt tgttttcaaa gttggcgtat aacatagtat cgacggagcc gattttgaaa
     1261 ccgcggtgat cacaggcagc aacgctctgt catcgttaca atcaacatgc taccctccgc
     1321 gagatcatcc gtgtttcaaa cccggcagct tagttgccgt tcttccgaat agcatcggta
     1381 acatgagcaa agtctgccgc cttacaacgg ctctcccgct gacgccgtcc cggactgatg
     1441 ggctgcctgt atcgagtggt gattttgtgc cgagctgccg gtcggggagc tgttggctgg
     1501 ctggtggcag gatatattgt ggtgtaaaca taacgaattg ctcttcacag aggagcaaat
     1561 gagtcacaca cattgttgag agacatatca aaataactca attgtaaggt aagttgatag
     1621 atttcccagt taaatgcatt attttaacaa tatttgtatc tcaaattttc aaaatcatat
     1681 caaatttgca gatcttgatg atgattcaca actctgtctt cgatttttga ttgttaacgg
     1741 gtatcgatat ttgggtaact taaaaagaat ttgctaaaga tcttaaaaaa cagattaaaa
     1801 aaacctttca aaatagctaa tcctttaaat ccgtttcaca atataatgct cattgatgat
     1861 aatcgaaaag attccatttt aaagtgcata taaattgctt aattatctct ttctgtctcg
     1921 taatattttg ttctctccta ttggatcgga cccgcatctt ctcgatcagt tcactatact
     1981 aatatttggt caacttcaag agagattaga acttcgttct ccaaagtgta aaggccgaca
     2041 agcagaaaag gaaacttcaa tcagaagcct tctaggtttt cccgcgtccc aaaaagcgaa
     2101 gggaggacgg ggaggacggt gaggccagaa gaagaaagca acagtaaaat ctcatgtgat
     2161 tgcgtgcatg tcatggatct atgggatgct agtccaccgt ttggaagtat gtgcagaagg
     2221 agaaaggcgc gggaggccaa aagcgacttc ggccacctct ctactttcta gaaagtttgc
     2281 tcgcagcttg taataagcgc ggaaaaggta ctcgaaaaac cggagcaatc ggtgtggtat
     2341 cagcagtcgg gttcgctaaa cctacgtctt cttattttgg aatgttaaat tgaaagggcg
     2401 tgccggccct aagcacggac gatgtgacag acggaggagt gtgagcaatt cggatgacga
     2461 tgctggccgg cagtcacgac aaagagtccg aaggggcatg gacagggcaa tatataaatg
     2521 gaaaagaggc tttgaatatt cctaaccctt cgtcgtcggc ctcccaactt gccctcgcat
     2581 gctgaattgg ccgcctatct gcgaatggcc ggccgccgac cctaagtcgc aataacttga
     2641 acggagccga tgcaaagtcc ttatcgaacg ctcgttatta taaacggggc agacctaggt
     2701 atttaattat atcaaaaccc tccgcaattt cgctaatcat tccctaattt cttctacttt
     2761 ggcgtctagt gctgtggtcg cgcttgctct caaagctcgc tgggaggcag tggagcgtct
     2821 ggcttaaaat cgatcgccgc aaccctagag ttcctttcac ttctttctct cttgcgtgga
     2881 gagcatcgac atcgaggagc tgctacttgt cttctggtat gcgccttgtg ccctcgtctc
     2941 tgtacgtcgt tacgattgct cgatattgtg attgtctaga cctgaaaccc gactggagct
     3001 tttacgtggt gtcgattggg gcctcgagcg agtgattttt taggttcgtt ttagccaagt
     3061 acatcccact ttggtcagtc ctgttatctc ggtaaggtgt tcagttcggt cactggtgcg
     3121 gaggtgcctc gtaagtttcc tgcatgatag atttgtctat ccttgacttg aagaggagtc
     3181 gaaggtcagc gaggttggct gtgtgctatg gacgtcggtg ctccttggtt ccgtgcttct
     3241 atgggtgttg cctcaacagt attgatgtgc ctgcagaaag gttgtcacca atggataaga
     3301 agtactctat cggactcgat atcggaacta actctgtggg atgggctgtg atcaccgatg
     3361 agtacaaggt gccatctaag aagttcaagg ttctcggaaa caccgatagg cactctatca
     3421 agaaaaacct tatcggtgct ctcctcttcg attctggtga aactgctgag gctaccagac
     3481 tcaagagaac cgctagaaga aggtacacca gaagaaagaa caggatctgc tacctccaag
     3541 agatcttctc taacgagatg gctaaagtgg atgattcatt cttccacagg ctcgaagagt
     3601 cattcctcgt ggaagaagat aagaagcacg agaggcaccc tatcttcgga aacatcgttg
     3661 atgaggtggc ataccacgag aagtacccta ctatctacca cctcagaaag aagctcgttg
     3721 attctactga taaggctgat ctcaggctca tctacctcgc tctcgctcac atgatcaagt
     3781 tcagaggaca cttcctcatc gagggtgatc tcaaccctga taactctgat gtggataagt
     3841 tgttcatcca gctcgtgcag acctacaacc agcttttcga agagaaccct atcaacgctt
     3901 caggtgtgga tgctaaggct atcctctctg ctaggctctc taagtcaaga aggcttgaga
     3961 acctcattgc tcagctccct ggtgagaaga agaacggact tttcggaaac ttgatcgctc
     4021 tctctctcgg actcacccct aacttcaagt ctaacttcga tctcgctgag gatgcaaagc
     4081 tccagctctc aaaggatacc tacgatgatg atctcgataa cctcctcgct cagatcggag
     4141 atcagtacgc tgatttgttc ctcgctgcta agaacctctc tgatgctatc ctcctcagtg
     4201 atatcctcag agtgaacacc gagatcacca aggctccact ctcagcttct atgatcaaga
     4261 gatacgatga gcaccaccag gatctcacac ttctcaaggc tcttgttaga cagcagctcc
     4321 cagagaagta caaagagatt ttcttcgatc agtctaagaa cggatacgct ggttacatcg
     4381 atggtggtgc atctcaagaa gagttctaca agttcatcaa gcctatcctc gagaagatgg
     4441 atggaaccga ggaactcctc gtgaagctca atagagagga tcttctcaga aagcagagga
     4501 ccttcgataa cggatctatc cctcatcaga tccacctcgg agagttgcac gctatcctta
     4561 gaaggcaaga ggatttctac ccattcctca aggataacag ggaaaagatt gagaagattc
     4621 tcaccttcag aatcccttac tacgtgggac ctctcgctag aggaaactca agattcgctt
     4681 ggatgaccag aaagtctgag gaaaccatca ccccttggaa cttcgaagag gtggtggata
     4741 agggtgctag tgctcagtct ttcatcgaga ggatgaccaa cttcgataag aaccttccaa
     4801 acgagaaggt gctccctaag cactctttgc tctacgagta cttcaccgtg tacaacgagt
     4861 tgaccaaggt taagtacgtg accgagggaa tgaggaagcc tgcttttttg tcaggtgagc
     4921 aaaagaaggc tatcgttgat ctcttgttca agaccaacag aaaggtgacc gtgaagcagc
     4981 tcaaagagga ttacttcaag aaaatcgagt gcttcgattc agttgagatt tctggtgttg
     5041 aggataggtt caacgcatct ctcggaacct accacgatct cctcaagatc attaaggata
     5101 aggatttctt ggataacgag gaaaacgagg atatcttgga ggatatcgtt cttaccctca
     5161 ccctctttga agatagagag atgattgaag aaaggctcaa gacctacgct catctcttcg
     5221 atgataaggt gatgaagcag ttgaagagaa gaagatacac tggttgggga aggctctcaa
     5281 gaaagctcat taacggaatc agggataagc agtctggaaa gacaatcctt gatttcctca
     5341 agtctgatgg attcgctaac agaaacttca tgcagctcat ccacgatgat tctctcacct
     5401 ttaaagagga tatccagaag gctcaggttt caggacaggg tgatagtctc catgagcata
     5461 tcgctaacct cgctggatct cctgcaatca agaagggaat cctccagact gtgaaggttg
     5521 tggatgagtt ggtgaaggtg atgggaaggc ataagcctga gaacatcgtg atcgaaatgg
     5581 ctagagagaa ccagaccact cagaagggac agaagaactc tagggaaagg atgaagagga
     5641 tcgaggaagg tatcaaagag cttggatctc agatcctcaa agagcaccct gttgagaaca
     5701 ctcagctcca gaatgagaag ctctacctct actacctcca gaacggaagg gatatgtatg
     5761 tggatcaaga gttggatatc aacaggctct ctgattacga tgttgatcat atcgtgccac
     5821 agtcattctt gaaggatgat tctatcgata acaaggtgct caccaggtct gataagaaca
     5881 ggggtaagag tgataacgtg ccaagtgaag aggttgtgaa gaaaatgaag aactattgga
     5941 ggcagctcct caacgctaag ctcatcactc agagaaagtt cgataacttg actaaggctg
     6001 agaggggagg actctctgaa ttggataagg caggattcat caagaggcag cttgtggaaa
     6061 ccaggcagat cactaagcac gttgcacaga tcctcgattc taggatgaac accaagtacg
     6121 atgagaacga taagttgatc agggaagtga aggttatcac cctcaagtca aagctcgtgt
     6181 ctgatttcag aaaggatttc caattctaca aggtgaggga aatcaacaac taccaccacg
     6241 ctcacgatgc ttaccttaac gctgttgttg gaaccgctct catcaagaag tatcctaagc
     6301 tcgagtcaga gttcgtgtac ggtgattaca aggtgtacga tgtgaggaag atgatcgcta
     6361 agtctgagca agagatcgga aaggctaccg ctaagtattt cttctactct aacatcatga
     6421 atttcttcaa gaccgagatt accctcgcta acggtgagat cagaaagagg ccactcatcg
     6481 agacaaacgg tgaaacaggt gagatcgtgt gggataaggg aagggatttc gctaccgtta
     6541 gaaaggtgct ctctatgcca caggtgaaca tcgttaagaa aaccgaggtg cagaccggtg
     6601 gattctctaa agagtctatc ctccctaaga ggaactctga taagctcatt gctaggaaga
     6661 aggattggga ccctaagaaa tacggtggtt tcgattctcc taccgtggct tactctgttc
     6721 tcgttgtggc taaggttgag aagggaaaga gtaagaagct caagtctgtt aaggaacttc
     6781 tcggaatcac tatcatggaa aggtcatctt tcgagaagaa cccaatcgat ttcctcgagg
     6841 ctaagggata caaagaggtt aagaaggatc tcatcatcaa gctcccaaag tactcactct
     6901 tcgaactcga gaacggtaga aagaggatgc tcgcttctgc tggtgagctt caaaagggaa
     6961 acgagcttgc tctcccatct aagtacgtta actttcttta cctcgcttct cactacgaga
     7021 agttgaaggg atctccagaa gataacgagc agaagcaact tttcgttgag cagcacaagc
     7081 actacttgga tgagatcatc gagcagatct ctgagttctc taaaagggtg atcctcgctg
     7141 atgcaaacct cgataaggtg ttgtctgctt acaacaagca cagagataag cctatcaggg
     7201 aacaggcaga gaacatcatc catctcttca cccttaccaa cctcggtgct cctgctgctt
     7261 tcaagtactt cgatacaacc atcgatagga agagatacac ctctaccaaa gaagtgctcg
     7321 atgctaccct catccatcag tctatcactg gactctacga gactaggatc gatctctcac
     7381 agctcggtgg tgattcaagg gctgatccta agaagaagag gaaggtttga gcttgagctc
     7441 gaatttcccc gatcgttcaa acatttggca ataaagtttc ttaagattga atcctgttgc
     7501 cggtcttgcg atgattatca tataatttct gttgaattac gttaagcatg taataattaa
     7561 catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc cgcaattata
     7621 catttaatac gcgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcgc
     7681 ggtgtcatct atgttactag atcgatccgt atcgatagcc tctagctaga gtcgatcgac
     7741 aagctcgagt ttctccataa taatgtgtga gtagttccca gataagggaa ttagggttcc
     7801 tatagggttt cgctcatgtg ttgagcatat aagaaaccct tagtatgtat ttgtatttgt
     7861 aaaatacttc tatcaataaa atttctaatt cctaaaatca aaatccagta ctaaaatcca
     7921 gatcgctagg ttgaagagca attctgacag gatatattgg cgggtaaacc taagagaaaa
     7981 gagcgtttat tagaataatc ggatatttaa aagggcgtga aaaggtttat ccgttcgtcc
     8041 atttgtatgt gcatgccaac cacagggttc ccctcgggat caaagtactt tgatccaacc
     8101 cctccgctgc tatagtgcag tcggcttctg acgttcagtg cagccgtcat ctgaaaacga
     8161 catgtcgcac aagtcctaag ttacgcgaca ggctgccgcc ctgccctttt cctggcgttt
     8221 tcttgtcgcg tgttttagtc gcataaagta gaatacttgc gactagaacc ggagacatta
     8281 cgccatgaac aagagcgccg ccgctggcct gctgggctat gcccgcgtca gcaccgacga
     8341 ccaggacttg accaaccaac gggccgaact gcacgcggcc ggctgcacca agctgttttc
     8401 cgagaagatc accggcacca ggcgcgaccg cccggagctg gccaggatgc ttgaccacct
     8461 acgccctggc gacgttgtga cagtgaccag gctagaccgc ctggcccgca gcacccgcga
     8521 cctactggac attgccgagc gcatccagga ggccggcgcg ggcctgcgta gcctggcaga
     8581 gccgtgggcc gacaccacca cgccggccgg ccgcatggtg ttgaccgtgt tcgccggcat
     8641 tgccgagttc gagcgttccc taatcatcga ccgcacccgg agcgggcgcg aggccgccaa
     8701 ggcccgaggc gtgaagtttg gcccccgccc taccctcacc ccggcacaga tcgcgcacgc
     8761 ccgcgagctg atcgaccagg aaggccgcac cgtgaaagag gcggctgcac tgcttggcgt
     8821 gcatcgctcg accctgtacc gcgcacttga gcgcagcgag gaagtgacgc ccaccgaggc
     8881 caggcggcgc ggtgccttcc gtgaggacgc attgaccgag gccgacgccc tggcggccgc
     8941 cgagaatgaa cgccaagagg aacaagcatg aaaccgcacc aggacggcca ggacgaaccg
     9001 tttttcatta ccgaagagat cgaggcggag atgatcgcgg ccgggtacgt gttcgagccg
     9061 cccgcgcacc tctcaaccgt gcggctgcat gaaatcctgg ccggtttgtc tgatgccaag
     9121 ctggcggcct ggccggccag cttggccgct gaagaaaccg agcgccgccg tctaaaaagg
     9181 tgatgtgtat ttgagtaaaa cagcttgcgt catgcggtcg ctgcgtatat gatgcgatga
     9241 gtaaataaac aaatacgcaa ggggaacgca tgaaggttat cgctgtactt aaccagaaag
     9301 gcgggtcagg caagacgacc atcgcaaccc atctagcccg cgccctgcaa ctcgccgggg
     9361 ccgatgttct gttagtcgat tccgatcccc agggcagtgc ccgcgattgg gcggccgtgc
     9421 gggaagatca accgctaacc gttgtcggca tcgaccgccc gacgattgac cgcgacgtga
     9481 aggccatcgg ccggcgcgac ttcgtagtga tcgacggagc gccccaggcg gcggacttgg
     9541 ctgtgtccgc gatcaaggca gccgacttcg tgctgattcc ggtgcagcca agcccttacg
     9601 acatatgggc caccgccgac ctggtggagc tggttaagca gcgcattgag gtcacggatg
     9661 gaaggctaca agcggccttt gtcgtgtcgc gggcgatcaa aggcacgcgc atcggcggtg
     9721 aggttgccga ggcgctggcc gggtacgagc tgcccattct tgagtcccgt atcacgcagc
     9781 gcgtgagcta cccaggcact gccgccgccg gcacaaccgt tcttgaatca gaacccgagg
     9841 gcgacgctgc ccgcgaggtc caggcgctgg ccgctgaaat taaatcaaaa ctcatttgag
     9901 ttaatgaggt aaagagaaaa tgagcaaaag cacaaacacg ctaagtgccg gccgtccgag
     9961 cgcacgcagc agcaaggctg caacgttggc cagcctggca gacacgccag ccatgaagcg
    10021 ggtcaacttt cagttgccgg cggaggatca caccaagctg aagatgtacg cggtacgcca
    10081 aggcaagacc attaccgagc tgctatctga atacatcgcg cagctaccag agtaaatgag
    10141 caaatgaata aatgagtaga tgaattttag cggctaaagg aggcggcatg gaaaatcaag
    10201 aacaaccagg caccgacgcc gtggaatgcc ccatgtgtgg aggaacgggc ggttggccag
    10261 gcgtaagcgg ctgggttgcc tgccggccct gcaatggcac tggaaccccc aagcccgagg
    10321 aatcggcgtg agcggtcgca aaccatccgg cccggtacaa atcggcgcgg cgctgggtga
    10381 tgacctggtg gagaagttga aggccgcgca ggccgcccag cggcaacgca tcgaggcaga
    10441 agcacgcccc ggtgaatcgt ggcaagcggc cgctgatcga atccgcaaag aatcccggca
    10501 accgccggca gccggtgcgc cgtcgattag gaagccgccc aagggcgacg agcaaccaga
    10561 ttttttcgtt ccgatgctct atgacgtggg cacccgcgat agtcgcagca tcatggacgt
    10621 ggccgttttc cgtctgtcga agcgtgaccg acgagctggc gaggtgatcc gctacgagct
    10681 tccagacggg cacgtagagg tttccgcagg gccggccggc atggccagtg tgtgggatta
    10741 cgacctggta ctgatggcgg tttcccatct aaccgaatcc atgaaccgat accgggaagg
    10801 gaagggagac aagcccggcc gcgtgttccg tccacacgtt gcggacgtac tcaagttctg
    10861 ccggcgagcc gatggcggaa agcagaaaga cgacctggta gaaacctgca ttcggttaaa
    10921 caccacgcac gttgccatgc agcgtacgaa gaaggccaag aacggccgcc tggtgacggt
    10981 atccgagggt gaagccttga ttagccgcta caagatcgta aagagcgaaa ccgggcggcc
    11041 ggagtacatc gagatcgagc tagctgattg gatgtaccgc gagatcacag aaggcaagaa
    11101 cccggacgtg ctgacggttc accccgatta ctttttgatc gatcccggca tcggccgttt
    11161 tctctaccgc ctggcacgcc gcgccgcagg caaggcagaa gccagatggt tgttcaagac
    11221 gatctacgaa cgcagtggca gcgccggaga gttcaagaag ttctgtttca ccgtgcgcaa
    11281 gctgatcggg tcaaatgacc tgccggagta cgatttgaag gaggaggcgg ggcaggctgg
    11341 cccgatccta gtcatgcgct accgcaacct gatcgagggc gaagcatccg ccggttccta
    11401 atgtacggag cagatgctag ggcaaattgc cctagcaggg gaaaaaggtc gaaaaggact
    11461 ctttcctgtg gatagcacgt acattgggaa cccaaagccg tacattggga accggaaccc
    11521 gtacattggg aacccaaagc cgtacattgg gaaccggtca cacatgtaag tgactgatat
    11581 aaaagagaaa aaaggcgatt tttccgccta aaactcttta aaacttatta aaactcttaa
    11641 aacccgcctg gcctgtgcat aactgtctgg ccagcgcaca gccgaagtgc tgcaaaaagc
    11701 gcctaccctt cggtcgctgc gctccctacg ccccgccgct tcgcgtcggc ctatcgcggc
    11761 cgctggccgc tcaaaaatgg ctggcctacg gccaggcaat ctaccagggc gcggacaagc
    11821 cgcgccgtcg ccactcgacc gccggcgccc acatcaaggc accctgcctc gcgcgtttcg
    11881 gtgatgacgg tgaaaacctc tgacacatgc agctcccggt gacggtcaca gcttgtctgt
    11941 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
    12001 ggggcgcagc catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc
    12061 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
    12121 cgtaaggaga aaataccgca tcaggcgctc atccgcttcc tcgctcactg actcgctgcg
    12181 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
    12241 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
    12301 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
    12361 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
    12421 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
    12481 atacctgtcc gcctttctcc cttcggaaag cgtggcgctt tctcatagct cacgctgtag
    12541 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
    12601 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
    12661 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
    12721 cggtgctaca gagttcttga agtggtggcc
//
