LOCUS       sequence-275264-        9098 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  L1 in position 3, to clone gRNA target using BbsI, lacZ blue-white 
            screening.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9098)
  AUTHORS   Sauret-Gueto S, Frangedakis E, Silvestri L, Rebmann M, Tomaselli M, 
            Markel K, Delmans M, West A, Patron NJ, Haseloff J
  TITLE     Systematic tools for reprogramming plant gene expression in a simple
            model, Marchantia polymorpha.
  JOURNAL   ACS Synth Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511.
  PUBMED    32163700
REFERENCE   2  (bases 1 to 9098)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511."
COMMENT     Sequence Label: L1_lacZgRNA-Ck3
FEATURES             Location/Qualifiers
     source          1..9098
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    2372..2396
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             2829..3644
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             2829..3644
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2902..2921)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3732..4320
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4221..4240
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4474..4491
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    4506..4646
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     4732..4754
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      4990..5184
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(5250..6323)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(6752..7381)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    8682..8706
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    8729..8753
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_RNA        complement(8765..8840)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    8902..8923
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8938..8943,8944..8961,8962..8968)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8976..8992
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8981..9003
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9000..9016
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9000..9016
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     misc_feature    9029..9085
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(join(9086..9098,1..5))
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(join(9086..9098,1..4))
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(join(9095..9098,1..19))
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
       61 ccccctttcg ccagctggcg taatagcgaa gaggcctgcg ccgatcgccc ttcccaacag
      121 ttgcgcagcc tgaatggcgg atgagcgcct gatgcggtat tttctcctta cgcatctgtg
      181 cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
      241 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
      301 ggcatccgct tacagacaag ctgtggaaga catcgagagg ctgggtgcaa ctctgcttta
      361 tatccaccat tattcagttc ttcactaaac gtatggtgca ataaatgagg ccagcgccac
      421 gtgtctcaac tgtggtcatt cttcgatcca ctgtgcactg tttgttgtct caagaagtca
      481 gattctggac ccaggaagaa acaagaaggt ggataaatcg accactcaaa agttctgaaa
      541 caagtgactg actagtgaac agcagttcat aaattccata tggttcgggg agacagagaa
      601 agagagcaat ccgaactcca aggaaactag ccggatacat ctctcgtgac gcttcgactt
      661 ttttcttatt tttgtgcagt tgcgtcactc tgagccatca aagggagaac tgaaaaggct
      721 gtgaagtgac tatgcgcgcg acttaaggta cctgggcatg catagaagca caagaaagtt
      781 tcttggaata agctcttgca gagatatgag atagaacttg cgttgaggag tgtactagag
      841 tgttcggaat ttgttcccat acatatatgt actgtactat agtttgacaa acatggagag
      901 tgaatagacg aaggtcctct tatactaacc gccactatat tgggatgcaa aaattgatca
      961 cgatggggac ttgtacatag agaattaatt tgtttgtttt ttagtttagg atgtttaata
     1021 atctcactgt ctaatgtcag gtggttgtgt ttttactttg tagtttgcgt gtgtgtgtgt
     1081 gtctggtttt cgtttctatt tgtaaatcgt cagacaatta aagatattgg aatagaaagt
     1141 tgtgagtttt ttgctagttg aagggtttct atagttctcg tttgaactgt gagagatcac
     1201 gaagtttatg attttgctta tagagtgata cgttatggac tgcattttat ctgtatattt
     1261 gacaccagta agtacagaat gctctataaa aagaaattaa gttttcagag taaaatgaaa
     1321 taagattgca catattcata ttatatttac atcatatgtg atgatttttt gaaaattttc
     1381 ttcgatattg tggattgttg acatatttga aaataaattt ttaactctta catataaatc
     1441 ttccataggt tttttttgac aatccacaat atactttctc aaagaaaaac ataaatcatg
     1501 tttttttcca taaaaaataa ataaatttgc aagatgtatg tgtcaaatag ttgattggat
     1561 taaaataaaa gcccattcac cttgaaacta tgttaaattt ttgtgaaaat caagataaac
     1621 cgacttttta tagtcagatt gttaaattct aattgagact tgcatgtgtt atttatctta
     1681 attattcatg aaaaatctga ttatattttt tattaatcag cacataagtt tgtgaaattt
     1741 tatgatcaat ccgattcatc aagttgaaga tatattacaa gctaatgagt ataacacatt
     1801 aactagcatt gttcaattgt tattgatact atgtatgtag tttagaaaaa ttaaaatgag
     1861 atgtagatga tgcagttttg acacatctta aacatgtagc ctggattaat ttacaaattt
     1921 ttgtagtcaa tcagtaatat gattgtattt ttcttgaaat ttacaacaaa aatgtgcttt
     1981 tgaaactatt attgttgtta ttttatcttc aagttttttt tctaaaaaat tataaacaat
     2041 gagagtatga taggaaaatt tgtttttttc attaaaaagc attatgatag ctgagtttgc
     2101 gatgttttga gctaaaaaat atttaaataa ataaagatat aaacatattt tgtctcgtgg
     2161 gttaaaatat atatgaaaga tcttagtata acattatgaa actgatatgt aatatatgat
     2221 ctaataataa attaaaattt gatcaaattt tttaacgagt tcaatttttt ttaaagttat
     2281 tatcatgacg tattagactt atgagaaaga aaaattgatc tttaaaaatc tcttttgaat
     2341 gaatactcct gtatgaagag caattcgtta tgtttacacc acaatatatc ctgccaccag
     2401 ccagccaaca gctccccgac cggcagctcg gcacaaaatc accactcgat acaggcagcc
     2461 catcagtccg ggacggcgtc agcgggagag ccgttgtaag gcggcagact ttgctcatgt
     2521 taccgatgct attcggaaga acggcaacta agctgccggg tttgaaacac ggatgatctc
     2581 gcggagggta gcatgttgat tgtaacgatg acagagcgtt gctgcctgtg atcaccgcgg
     2641 tttcaaaatc ggctccgtcg atactatgtt atacgccaac tttgaaaaca actttgaaaa
     2701 agctgttttc tggtatttaa ggttttagaa tgcaaggaac agtgaattgg agttcgtctt
     2761 gttataatta gcttcttggg gtatctttaa atactgtaga aaagaggaag gaaataataa
     2821 gttctgttat gagccatatt caacgggaaa cgtcttgctc aaggccgcga ttaaattcca
     2881 acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg
     2941 cgacaatcta ccgattgtat gggaagcccg atgcgccaga gttgtttctg aaacatggca
     3001 aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg ctgacggaat
     3061 ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca tggttactca
     3121 ccactgcgat cccagggaaa acagcattcc aggtattaga agaatatcct gattcaggtg
     3181 aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta
     3241 attgtccttt taacagcgat cgcgtatttc gactcgctca ggcgcaatca cgaatgaata
     3301 acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct gttgaacaag
     3361 tctggaaaga aatgcataaa cttttgccat tctcaccgga ttcagtcgtc actcatggtg
     3421 atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt attgatgttg
     3481 gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac tgcctcggtg
     3541 agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat aatcctgata
     3601 tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaatcacct agaatgcatg
     3661 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     3721 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     3781 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     3841 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     3901 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     3961 ccagtggctg ctgccagtgg cgataagttg tgtcttaccg ggttggactc aagacgatag
     4021 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     4081 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     4141 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     4201 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     4261 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     4321 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     4381 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     4441 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggat
     4501 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg
     4561 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat
     4621 cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct
     4681 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc accgggagct
     4741 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagggt gccttgatgt
     4801 gggcgccggc ggtcgagtgg cgacggcgcg gcttgtccgc gccctggtag attgcctggc
     4861 cgtaggccag ccatttttga gcggccagcg gccgcgatag gccgacgcga agcggcgggg
     4921 cgtagggagc gcagcgaccg aagggtaggc gctttttgca gcacttcggc tgtgcgctgg
     4981 ccagacagtt atgcacaggc caggcgggtt ttaagagttt taataagttt taaagagttt
     5041 taggcggaaa aatcgccttt tttctctttt atatcagtca cttacatgtg tgaccggttc
     5101 ccaatgtacg gctttgggtt cccaatgtac gggttccggt tcccaatgta cggctttggg
     5161 ttcccaatgt acgtgctatc cacaggaaag agtccttttc gacctttttc ccctgctagg
     5221 gcaatttgcc ctagcatctg ctccgtacat taggaaccgg cggatgcttc gccctcgatc
     5281 aggttgcggt agcgcatgac taggatcggg ccagcctgcc ccgcctcctc cttcaaatcg
     5341 tactccggca ggtcatttga cccgatcagc ttgcgcacgg tgaaacagaa cttcttgaac
     5401 tctccggcgc tgccactgcg ttcgtagatc gtcttgaaca accatctggc ttctgccttg
     5461 cctgcggcgc ggcgtgccag gcggtagaga aaacggccga tgccgggatc gatcaaaaag
     5521 taatcggggt gaaccgtcag cacgtccggg ttcttgcctt ctgtgatctc gcggtacatc
     5581 caatcagcta gctcgatctc gatgtactcc ggccgcccgg tttcgctctt tacgatcttg
     5641 tagcggctaa tcaaggcttc accctcggat accgtcacca ggcggccgtt cttggccttc
     5701 ttcgtacgct gcatggcaac gtgcgtggtg tttaaccgaa tgcaggtttc taccaggtcg
     5761 tctttctgct ttccgccatc ggctcgccgg cagaacttga gtacgtccgc aacgtgtgga
     5821 cggaacacgc ggccgggctt gtctcccttc ccttcccggt atcggttcat ggattcggtt
     5881 agatgggaaa ccgccatcag taccaggtcg taatcccaca cactggccat gccggccggc
     5941 cctgcggaaa cctctacgtg cccgtctgga agctcgtagc ggatcacctc gccagctcgt
     6001 cggtcacgct tcgacagacg gaaaacggcc acgtccatga tgctgcgact atcgcgggtg
     6061 cccacgtcat agagcatcgg aacgaaaaaa tctggttgct cgtcgccctt gggcggcttc
     6121 ctaatcgacg gcgcaccggc tgccggcggt tgccgggatt ctttgcggat tcgatcagcg
     6181 gccgcttgcc acgattcacc ggggcgtgct tctgcctcga tgcgttgccg ctgggcggcc
     6241 tgcgcggcct tcaacttctc caccaggtca tcacccagcg ccgcgccgat ttgtaccggg
     6301 ccggatggtt tgcgaccgct cacgccgatt cctcgggctt gggggttcca gtgccattgc
     6361 agggccggca ggcaacccag ccgcttacgc ctggccaacc gcccgttcct ccacacatgg
     6421 ggcattccac ggcgtcggtg cctggttgtt cttgattttc catgccgcct cctttagccg
     6481 ctaaaattca tctactcatt tattcatttg ctcatttact ctggtagctg cgcgatgtat
     6541 tcagatagca gctcggtaat ggtcttgcct tggcgtaccg cgtacatctt cagcttggtg
     6601 tgatcctccg ccggcaactg aaagttgacc cgcttcatgg ctggcgtgtc tgccaggctg
     6661 gccaacgttg cagccttgct gctgcgtgcg ctcggacggc cggcacttag cgtgtttgtg
     6721 cttttgctca ttttctcttt acctcattaa ctcaaatgag ttttgattta atttcagcgg
     6781 ccagcgcctg gacctcgcgg gcagcgtcgc cctcgggttc tgattcaaga acggttgtgc
     6841 cggcggcggc agtgcctggg tagctcacgc gctgcgtgat acgggactca agaatgggca
     6901 gctcgtaccc ggccagcgcc tcggcaacct caccgccgat gcgcgtgcct ttgatcgccc
     6961 gcgacacgac aaaggccgct tgtagccttc catccgtgac ctcaatgcgc tgcttaacca
     7021 gctccaccag gtcggcggtg gcccatatgt cgtaagggct tggctgcacc ggaatcagca
     7081 cgaagtcggc tgccttgatc gcggacacag ccaagtccgc cgcctggggc gctccgtcga
     7141 tcactacgaa gtcgcgccgg ccgatggcct tcacgtcgcg gtcaatcgtc gggcggtcga
     7201 tgccgacaac ggttagcggt tgatcttccc gcacggccgc ccaatcgcgg gcactgccct
     7261 ggggatcgga atcgactaac agaacatcgg ccccggcgag ttgcagggcg cgggctagat
     7321 gggttgcgat ggtcgtcttg cctgacccgc ctttctggtt aagtacagcg ataaccttca
     7381 tgcgttcccc ttgcgtattt gtttatttac tcatcgcatc atatacgcag cgaccgcatg
     7441 acgcaagctg ttttactcaa atacacatca cctttttaga cggcggcgct cggtttcttc
     7501 agcggccaag ctggccggcc aggccgccag cttggcatca gacaaaccgg ccaggatttc
     7561 atgcagccgc acggttgaga ggtgcgcggg cggctcgaac acgtacccgg ccgcgatcat
     7621 ctccgcctcg atctcttcgg taatgaaaaa cggttcgtcc tggccgtcct ggtgcggttt
     7681 catgcttgtt cctcttggcg ttcattctcg gcggccgcca gggcgtcggc ctcggtcaat
     7741 gcgtcctcac ggaaggcacc gcgccgcctg gcctcggtgg gcgtcacttc ctcgctgcgc
     7801 tcaagtgcgc ggtacagggt cgagcgatgc acgccaagca gtgcagccgc ctctttcacg
     7861 gtgcggcctt cctggtcgat cagctcgcgg gcgtgcgcga tctgtgccgg ggtgagggta
     7921 gggcgggggc caaacttcac gcctcgggcc ttggcggcct cgcgcccgct ccgggtgcgg
     7981 tcgatgatta gggaacgctc gaactcggca atgccggcga acacggtcaa caccatgcgg
     8041 ccggccggcg tggtggtgtc ggcccacggc tctgccaggc tacgcaggcc cgcgccggcc
     8101 tcctggatgc gctcggcaat gtccagtagg tcgcgggtgc tgcgggccag gcggtctagc
     8161 ctggtcactg tcacaacgtc gccagggcgt aggtggtcaa gcatcctggc cagctccggg
     8221 cggtcgcgcc tggtgccggt gatcttctcg gaaaacagct tggtgcagcc ggccgcgtgc
     8281 agttcggccc gttggttggt caagtcctgg tcgtcggtgc tgacgcgggc atagcccagc
     8341 aggccagcgg cggcgctctt gttcatggcg taatgtctcc ggttctagtc gcaagtattc
     8401 tactttatgc gactaaaaca cgcgacaaga aaacgccagg aaaagggcag ggcggcagcc
     8461 tgtcgcgtaa cttaggactt gtgcgacatg tcgttttcag atgacggctg cactgaacgt
     8521 cagaagccga ctgcactata gcagcggagg ggttggatca aagtactttg atcccgaggg
     8581 gaaccctgtg gttggcatgc acatacaaat ggacgaacgg ataaaccttt tcacgccctt
     8641 ttaaatatcc gattattcta ataaacgctc ttttctctta ggtttacccg ccaatatatc
     8701 ctgtcagaat tgctcttcac tgtagcggcc aactttgtac aagaaagctg ggtctagaaa
     8761 aaaagcaccg actcggtgcc actttttcaa gttgataacg gactagcctt attttaactt
     8821 gctatttcta gctctaaaac gtcttcccag ctggcacgac aggtttcccg actggaaagc
     8881 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
     8941 acactatatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
     9001 aggaaacagc tatgaccatg attacgccaa gcttgcatgc ctgcaggtcg actctagagg
     9061 atccccgggt accgagctcg aattcactgg ccgtcgtt
//
