LOCUS       sequence-275266-        9098 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  L1 in position 2, to clone gRNA target using BbsI, lacZ blue-white 
            screening.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9098)
  AUTHORS   Sauret-Gueto S, Frangedakis E, Silvestri L, Rebmann M, Tomaselli M, 
            Markel K, Delmans M, West A, Patron NJ, Haseloff J
  TITLE     Systematic tools for reprogramming plant gene expression in a simple
            model, Marchantia polymorpha.
  JOURNAL   ACS Synth Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511.
  PUBMED    32163700
REFERENCE   2  (bases 1 to 9098)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511."
COMMENT     Sequence Label: L1_lacZgRNA-Ck2
FEATURES             Location/Qualifiers
     source          1..9098
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1897..1921
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             2354..3169
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             2354..3169
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2427..2446)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3257..3845
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3746..3765
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3999..4016
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    4031..4171
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     4257..4279
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      4515..4709
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(4775..5848)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(6277..6906)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    8207..8231
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    8254..8278
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_RNA        complement(8290..8365)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    8427..8448
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8463..8468,8469..8486,8487..8493)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8501..8517
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8506..8528
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8525..8541
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8525..8541
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     misc_feature    8554..8610
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(8611..8628)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8611..8627)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8620..8642)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 agtcagattc tggacccagg aagaaacaag aaggtggata aatcgaccac tcaaaagttc
       61 tgaaacaagt gactgactag tgaacagcag ttcataaatt ccatatggtt cggggagaca
      121 gagaaagaga gcaatccgaa ctccaaggaa actagccgga tacatctctc gtgacgcttc
      181 gacttttttc ttatttttgt gcagttgcgt cactctgagc catcaaaggg agaactgaaa
      241 aggctgtgaa gtgactatgc gcgcgactta aggtacctgg gcatgcatag aagcacaaga
      301 aagtttcttg gaataagctc ttgcagagat atgagataga acttgcgttg aggagtgtac
      361 tagagtgttc ggaatttgtt cccatacata tatgtactgt actatagttt gacaaacatg
      421 gagagtgaat agacgaaggt cctcttatac taaccgccac tatattggga tgcaaaaatt
      481 gatcacgatg gggacttgta catagagaat taatttgttt gttttttagt ttaggatgtt
      541 taataatctc actgtctaat gtcaggtggt tgtgttttta ctttgtagtt tgcgtgtgtg
      601 tgtgtgtctg gttttcgttt ctatttgtaa atcgtcagac aattaaagat attggaatag
      661 aaagttgtga gttttttgct agttgaaggg tttctatagt tctcgtttga actgtgagag
      721 atcacgaagt ttatgatttt gcttatagag tgatacgtta tggactgcat tttatctgta
      781 tatttgacac cagtaagtac agaatgctct ataaaaagaa attaagtttt cagagtaaaa
      841 tgaaataaga ttgcacatat tcatattata tttacatcat atgtgatgat tttttgaaaa
      901 ttttcttcga tattgtggat tgttgacata tttgaaaata aatttttaac tcttacatat
      961 aaatcttcca taggtttttt ttgacaatcc acaatatact ttctcaaaga aaaacataaa
     1021 tcatgttttt ttccataaaa aataaataaa tttgcaagat gtatgtgtca aatagttgat
     1081 tggattaaaa taaaagccca ttcaccttga aactatgtta aatttttgtg aaaatcaaga
     1141 taaaccgact ttttatagtc agattgttaa attctaattg agacttgcat gtgttattta
     1201 tcttaattat tcatgaaaaa tctgattata ttttttatta atcagcacat aagtttgtga
     1261 aattttatga tcaatccgat tcatcaagtt gaagatatat tacaagctaa tgagtataac
     1321 acattaacta gcattgttca attgttattg atactatgta tgtagtttag aaaaattaaa
     1381 atgagatgta gatgatgcag ttttgacaca tcttaaacat gtagcctgga ttaatttaca
     1441 aatttttgta gtcaatcagt aatatgattg tatttttctt gaaatttaca acaaaaatgt
     1501 gcttttgaaa ctattattgt tgttatttta tcttcaagtt ttttttctaa aaaattataa
     1561 acaatgagag tatgatagga aaatttgttt ttttcattaa aaagcattat gatagctgag
     1621 tttgcgatgt tttgagctaa aaaatattta aataaataaa gatataaaca tattttgtct
     1681 cgtgggttaa aatatatatg aaagatctta gtataacatt atgaaactga tatgtaatat
     1741 atgatctaat aataaattaa aatttgatca aattttttaa cgagttcaat tttttttaaa
     1801 gttattatca tgacgtatta gacttatgag aaagaaaaat tgatctttaa aaatctcttt
     1861 tgaatgaata ctccttgctg aagagcaatt cgttatgttt acaccacaat atatcctgcc
     1921 accagccagc caacagctcc ccgaccggca gctcggcaca aaatcaccac tcgatacagg
     1981 cagcccatca gtccgggacg gcgtcagcgg gagagccgtt gtaaggcggc agactttgct
     2041 catgttaccg atgctattcg gaagaacggc aactaagctg ccgggtttga aacacggatg
     2101 atctcgcgga gggtagcatg ttgattgtaa cgatgacaga gcgttgctgc ctgtgatcac
     2161 cgcggtttca aaatcggctc cgtcgatact atgttatacg ccaactttga aaacaacttt
     2221 gaaaaagctg ttttctggta tttaaggttt tagaatgcaa ggaacagtga attggagttc
     2281 gtcttgttat aattagcttc ttggggtatc tttaaatact gtagaaaaga ggaaggaaat
     2341 aataagttct gttatgagcc atattcaacg ggaaacgtct tgctcaaggc cgcgattaaa
     2401 ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc
     2461 aggtgcgaca atctaccgat tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca
     2521 tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa actggctgac
     2581 ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt
     2641 actcaccact gcgatcccag ggaaaacagc attccaggta ttagaagaat atcctgattc
     2701 aggtgaaaat attgttgatg cgctggcagt gttcctgcgc cggttgcatt cgattcctgt
     2761 ttgtaattgt ccttttaaca gcgatcgcgt atttcgactc gctcaggcgc aatcacgaat
     2821 gaataacggt ttggttgatg cgagtgattt tgatgacgag cgtaatggct ggcctgttga
     2881 acaagtctgg aaagaaatgc ataaactttt gccattctca ccggattcag tcgtcactca
     2941 tggtgatttc tcacttgata accttatttt tgacgagggg aaattaatag gttgtattga
     3001 tgttggacga gtcggaatcg cagaccgata ccaggatctt gccatcctat ggaactgcct
     3061 cggtgagttt tctccttcat tacagaaacg gctttttcaa aaatatggta ttgataatcc
     3121 tgatatgaat aaattgcagt ttcatttgat gctcgatgag tttttctaat cacctagaat
     3181 gcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     3241 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     3301 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     3361 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     3421 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     3481 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     3541 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     3601 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     3661 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     3721 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     3781 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     3841 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     3901 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     3961 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     4021 cggatgagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     4081 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc
     4141 gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc
     4201 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtcaccgg
     4261 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agggtgcctt
     4321 gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg tccgcgccct ggtagattgc
     4381 ctggccgtag gccagccatt tttgagcggc cagcggccgc gataggccga cgcgaagcgg
     4441 cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt ttgcagcact tcggctgtgc
     4501 gctggccaga cagttatgca caggccaggc gggttttaag agttttaata agttttaaag
     4561 agttttaggc ggaaaaatcg ccttttttct cttttatatc agtcacttac atgtgtgacc
     4621 ggttcccaat gtacggcttt gggttcccaa tgtacgggtt ccggttccca atgtacggct
     4681 ttgggttccc aatgtacgtg ctatccacag gaaagagtcc ttttcgacct ttttcccctg
     4741 ctagggcaat ttgccctagc atctgctccg tacattagga accggcggat gcttcgccct
     4801 cgatcaggtt gcggtagcgc atgactagga tcgggccagc ctgccccgcc tcctccttca
     4861 aatcgtactc cggcaggtca tttgacccga tcagcttgcg cacggtgaaa cagaacttct
     4921 tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt gaacaaccat ctggcttctg
     4981 ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg gccgatgccg ggatcgatca
     5041 aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt gccttctgtg atctcgcggt
     5101 acatccaatc agctagctcg atctcgatgt actccggccg cccggtttcg ctctttacga
     5161 tcttgtagcg gctaatcaag gcttcaccct cggataccgt caccaggcgg ccgttcttgg
     5221 ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa ccgaatgcag gtttctacca
     5281 ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa cttgagtacg tccgcaacgt
     5341 gtggacggaa cacgcggccg ggcttgtctc ccttcccttc ccggtatcgg ttcatggatt
     5401 cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc ccacacactg gccatgccgg
     5461 ccggccctgc ggaaacctct acgtgcccgt ctggaagctc gtagcggatc acctcgccag
     5521 ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc catgatgctg cgactatcgc
     5581 gggtgcccac gtcatagagc atcggaacga aaaaatctgg ttgctcgtcg cccttgggcg
     5641 gcttcctaat cgacggcgca ccggctgccg gcggttgccg ggattctttg cggattcgat
     5701 cagcggccgc ttgccacgat tcaccggggc gtgcttctgc ctcgatgcgt tgccgctggg
     5761 cggcctgcgc ggccttcaac ttctccacca ggtcatcacc cagcgccgcg ccgatttgta
     5821 ccgggccgga tggtttgcga ccgctcacgc cgattcctcg ggcttggggg ttccagtgcc
     5881 attgcagggc cggcaggcaa cccagccgct tacgcctggc caaccgcccg ttcctccaca
     5941 catggggcat tccacggcgt cggtgcctgg ttgttcttga ttttccatgc cgcctccttt
     6001 agccgctaaa attcatctac tcatttattc atttgctcat ttactctggt agctgcgcga
     6061 tgtattcaga tagcagctcg gtaatggtct tgccttggcg taccgcgtac atcttcagct
     6121 tggtgtgatc ctccgccggc aactgaaagt tgacccgctt catggctggc gtgtctgcca
     6181 ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg acggccggca cttagcgtgt
     6241 ttgtgctttt gctcattttc tctttacctc attaactcaa atgagttttg atttaatttc
     6301 agcggccagc gcctggacct cgcgggcagc gtcgccctcg ggttctgatt caagaacggt
     6361 tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc gtgatacggg actcaagaat
     6421 gggcagctcg tacccggcca gcgcctcggc aacctcaccg ccgatgcgcg tgcctttgat
     6481 cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc gtgacctcaa tgcgctgctt
     6541 aaccagctcc accaggtcgg cggtggccca tatgtcgtaa gggcttggct gcaccggaat
     6601 cagcacgaag tcggctgcct tgatcgcgga cacagccaag tccgccgcct ggggcgctcc
     6661 gtcgatcact acgaagtcgc gccggccgat ggccttcacg tcgcggtcaa tcgtcgggcg
     6721 gtcgatgccg acaacggtta gcggttgatc ttcccgcacg gccgcccaat cgcgggcact
     6781 gccctgggga tcggaatcga ctaacagaac atcggccccg gcgagttgca gggcgcgggc
     6841 tagatgggtt gcgatggtcg tcttgcctga cccgcctttc tggttaagta cagcgataac
     6901 cttcatgcgt tccccttgcg tatttgttta tttactcatc gcatcatata cgcagcgacc
     6961 gcatgacgca agctgtttta ctcaaataca catcaccttt ttagacggcg gcgctcggtt
     7021 tcttcagcgg ccaagctggc cggccaggcc gccagcttgg catcagacaa accggccagg
     7081 atttcatgca gccgcacggt tgagaggtgc gcgggcggct cgaacacgta cccggccgcg
     7141 atcatctccg cctcgatctc ttcggtaatg aaaaacggtt cgtcctggcc gtcctggtgc
     7201 ggtttcatgc ttgttcctct tggcgttcat tctcggcggc cgccagggcg tcggcctcgg
     7261 tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc ggtgggcgtc acttcctcgc
     7321 tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc aagcagtgca gccgcctctt
     7381 tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg cgcgatctgt gccggggtga
     7441 gggtagggcg ggggccaaac ttcacgcctc gggccttggc ggcctcgcgc ccgctccggg
     7501 tgcggtcgat gattagggaa cgctcgaact cggcaatgcc ggcgaacacg gtcaacacca
     7561 tgcggccggc cggcgtggtg gtgtcggccc acggctctgc caggctacgc aggcccgcgc
     7621 cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg ggtgctgcgg gccaggcggt
     7681 ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg gtcaagcatc ctggccagct
     7741 ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa cagcttggtg cagccggccg
     7801 cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc ggtgctgacg cgggcatagc
     7861 ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg tctccggttc tagtcgcaag
     7921 tattctactt tatgcgacta aaacacgcga caagaaaacg ccaggaaaag ggcagggcgg
     7981 cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt ttcagatgac ggctgcactg
     8041 aacgtcagaa gccgactgca ctatagcagc ggaggggttg gatcaaagta ctttgatccc
     8101 gaggggaacc ctgtggttgg catgcacata caaatggacg aacggataaa ccttttcacg
     8161 cccttttaaa tatccgatta ttctaataaa cgctcttttc tcttaggttt acccgccaat
     8221 atatcctgtc agaattgctc ttcagtatag cggccaactt tgtacaagaa agctgggtct
     8281 agaaaaaaag caccgactcg gtgccacttt ttcaagttga taacggacta gccttatttt
     8341 aacttgctat ttctagctct aaaacgtctt cccagctggc acgacaggtt tcccgactgg
     8401 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     8461 gctttacact atatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
     8521 cacacaggaa acagctatga ccatgattac gccaagcttg catgcctgca ggtcgactct
     8581 agaggatccc cgggtaccga gctcgaattc actggccgtc gttttacaac gtcgtgactg
     8641 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     8701 gcgtaatagc gaagaggcct gcgccgatcg cccttcccaa cagttgcgca gcctgaatgg
     8761 cggatgagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     8821 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc
     8881 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
     8941 aagctgtgga agacatcgag aggctgggtg caactctgct ttatatccac cattattcag
     9001 ttcttcacta aacgtatggt gcaataaatg aggccagcgc cacgtgtctc aactgtggtc
     9061 attcttcgat ccactgtgca ctgtttgttg tctcaaga
//
