LOCUS       sequence-275272-       18117 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid to accept gRNA target with SapI, lacZ blue-white screening. 
            Contains Cas9 and HygR..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18117)
  AUTHORS   Sauret-Gueto S, Frangedakis E, Silvestri L, Rebmann M, Tomaselli M, 
            Markel K, Delmans M, West A, Patron NJ, Haseloff J
  TITLE     Systematic tools for reprogramming plant gene expression in a simple
            model, Marchantia polymorpha.
  JOURNAL   ACS Synth Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511.
  PUBMED    32163700
REFERENCE   2  (bases 1 to 18117)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511."
COMMENT     Sequence Label: L2_lacZgRNA-Cas9-CsA
FEATURES             Location/Qualifiers
     source          1..18117
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     455..477
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     469..486
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     470..486
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    487..543
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(556..572)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(556..572)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(569..591)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    580..596
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(604..610,611..628,629..634))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    649..670
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    complement(819..843)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2593..6696
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             2593..6696
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6709..6729
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      6753..7005
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     polyA_signal    7052..7226
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    7252..7276
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             8577..9206
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             9635..10708
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      10774..10968
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(11204..11226)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    11312..11452
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(11467..11484)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11638..12226)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11718..11737)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(12314..13105)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    13891..13915
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        14037..14713
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     14650..14670
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             14786..15811
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    15846..16020
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
ORIGIN
        1 ttgagacaac aaacagtgca cagtggatcg aagaatgacc acagttgaga cacgtggcgc
       61 tggcctcatt tattgcacca tacgtttagt gaagaactga ataatggtgg atataaagca
      121 gagttgcacc cagcctctcg tgaagagcca cagcttgtct gtaagcggat gccgggagca
      181 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg
      241 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
      301 gcgtaaggag aaaataccgc atcaggcgct catccgccat tcaggctgcg caactgttgg
      361 gaagggcgat cggcgcaggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct
      421 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg
      481 gccagtgaat tcgagctcgg tacccgggga tcctctagag tcgacctgca ggcatgcaag
      541 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
      601 acacaacata cgagccggaa gcatatagtg taaagcctgg ggtgcctaat gagtgagcta
      661 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
      721 gctgggctct tcatttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa
      781 cttgaaaaag tggcaccgag tcggtgcttt ttttctagac ccagctttct tgtacaaagt
      841 tggccgctac agaggagcaa atgagtcaca cacattgttg agagacatat caaaataact
      901 caattgtaag gtaagttgat agatttccca gttaaatgca ttattttaac aatatttgta
      961 tctcaaattt tcaaaatcat atcaaatttg cagatcttga tgatgattca caactctgtc
     1021 ttcgattttt gattgttaac gggtatcgat atttgggtaa cttaaaaaga atttgctaaa
     1081 gatcttaaaa aacagattaa aaaaaccttt caaaatagct aatcctttaa atccgtttca
     1141 caatataatg ctcattgatg ataatcgaaa agattccatt ttaaagtgca tataaattgc
     1201 ttaattatct ctttctgtct cgtaatattt tgttctctcc tattggatcg gacccgcatc
     1261 ttctcgatca gttcactata ctaatatttg gtcaacttca agagagatta gaacttcgtt
     1321 ctccaaagtg taaaggccga caagcagaaa aggaaacttc aatcagaagc cttctaggtt
     1381 ttcccgcgtc ccaaaaagcg aagggaggac ggggaggacg gtgaggccag aagaagaaag
     1441 caacagtaaa atctcatgtg attgcgtgca tgtcatggat ctatgggatg ctagtccacc
     1501 gtttggaagt atgtgcagaa ggagaaaggc gcgggaggcc aaaagcgact tcggccacct
     1561 ctctactttc tagaaagttt gctcgcagct tgtaataagc gcggaaaagg tactcgaaaa
     1621 accggagcaa tcggtgtggt atcagcagtc gggttcgcta aacctacgtc ttcttatttt
     1681 ggaatgttaa attgaaaggg cgtgccggcc ctaagcacgg acgatgtgac agacggagga
     1741 gtgtgagcaa ttcggatgac gatgctggcc ggcagtcacg acaaagagtc cgaaggggca
     1801 tggacagggc aatatataaa tggaaaagag gctttgaata ttcctaaccc ttcgtcgtcg
     1861 gcctcccaac ttgccctcgc atgctgaatt ggccgcctat ctgcgaatgg ccggccgccg
     1921 accctaagtc gcaataactt gaacggagcc gatgcaaagt ccttatcgaa cgctcgttat
     1981 tataaacggg gcagacctag gtatttaatt atatcaaaac cctccgcaat ttcgctaatc
     2041 attccctaat ttcttctact ttggcgtcta gtgctgtggt cgcgcttgct ctcaaagctc
     2101 gctgggaggc agtggagcgt ctggcttaaa atcgatcgcc gcaaccctag agttcctttc
     2161 acttctttct ctcttgcgtg gagagcatcg acatcgagga gctgctactt gtcttctggt
     2221 atgcgccttg tgccctcgtc tctgtacgtc gttacgattg ctcgatattg tgattgtcta
     2281 gacctgaaac ccgactggag cttttacgtg gtgtcgattg gggcctcgag cgagtgattt
     2341 tttaggttcg ttttagccaa gtacatccca ctttggtcag tcctgttatc tcggtaaggt
     2401 gttcagttcg gtcactggtg cggaggtgcc tcgtaagttt cctgcatgat agatttgtct
     2461 atccttgact tgaagaggag tcgaaggtca gcgaggttgg ctgtgtgcta tggacgtcgg
     2521 tgctccttgg ttccgtgctt ctatgggtgt tgcctcaaca gtattgatgt gcctgcagaa
     2581 aggttgtcac caatggataa gaagtactct atcggactcg atatcggaac taactctgtg
     2641 ggatgggctg tgatcaccga tgagtacaag gtgccatcta agaagttcaa ggttctcgga
     2701 aacaccgata ggcactctat caagaaaaac cttatcggtg ctctcctctt cgattctggt
     2761 gaaactgctg aggctaccag actcaagaga accgctagaa gaaggtacac cagaagaaag
     2821 aacaggatct gctacctcca agagatcttc tctaacgaga tggctaaagt ggatgattca
     2881 ttcttccaca ggctcgaaga gtcattcctc gtggaagaag ataagaagca cgagaggcac
     2941 cctatcttcg gaaacatcgt tgatgaggtg gcataccacg agaagtaccc tactatctac
     3001 cacctcagaa agaagctcgt tgattctact gataaggctg atctcaggct catctacctc
     3061 gctctcgctc acatgatcaa gttcagagga cacttcctca tcgagggtga tctcaaccct
     3121 gataactctg atgtggataa gttgttcatc cagctcgtgc agacctacaa ccagcttttc
     3181 gaagagaacc ctatcaacgc ttcaggtgtg gatgctaagg ctatcctctc tgctaggctc
     3241 tctaagtcaa gaaggcttga gaacctcatt gctcagctcc ctggtgagaa gaagaacgga
     3301 cttttcggaa acttgatcgc tctctctctc ggactcaccc ctaacttcaa gtctaacttc
     3361 gatctcgctg aggatgcaaa gctccagctc tcaaaggata cctacgatga tgatctcgat
     3421 aacctcctcg ctcagatcgg agatcagtac gctgatttgt tcctcgctgc taagaacctc
     3481 tctgatgcta tcctcctcag tgatatcctc agagtgaaca ccgagatcac caaggctcca
     3541 ctctcagctt ctatgatcaa gagatacgat gagcaccacc aggatctcac acttctcaag
     3601 gctcttgtta gacagcagct cccagagaag tacaaagaga ttttcttcga tcagtctaag
     3661 aacggatacg ctggttacat cgatggtggt gcatctcaag aagagttcta caagttcatc
     3721 aagcctatcc tcgagaagat ggatggaacc gaggaactcc tcgtgaagct caatagagag
     3781 gatcttctca gaaagcagag gaccttcgat aacggatcta tccctcatca gatccacctc
     3841 ggagagttgc acgctatcct tagaaggcaa gaggatttct acccattcct caaggataac
     3901 agggaaaaga ttgagaagat tctcaccttc agaatccctt actacgtggg acctctcgct
     3961 agaggaaact caagattcgc ttggatgacc agaaagtctg aggaaaccat caccccttgg
     4021 aacttcgaag aggtggtgga taagggtgct agtgctcagt ctttcatcga gaggatgacc
     4081 aacttcgata agaaccttcc aaacgagaag gtgctcccta agcactcttt gctctacgag
     4141 tacttcaccg tgtacaacga gttgaccaag gttaagtacg tgaccgaggg aatgaggaag
     4201 cctgcttttt tgtcaggtga gcaaaagaag gctatcgttg atctcttgtt caagaccaac
     4261 agaaaggtga ccgtgaagca gctcaaagag gattacttca agaaaatcga gtgcttcgat
     4321 tcagttgaga tttctggtgt tgaggatagg ttcaacgcat ctctcggaac ctaccacgat
     4381 ctcctcaaga tcattaagga taaggatttc ttggataacg aggaaaacga ggatatcttg
     4441 gaggatatcg ttcttaccct caccctcttt gaagatagag agatgattga agaaaggctc
     4501 aagacctacg ctcatctctt cgatgataag gtgatgaagc agttgaagag aagaagatac
     4561 actggttggg gaaggctctc aagaaagctc attaacggaa tcagggataa gcagtctgga
     4621 aagacaatcc ttgatttcct caagtctgat ggattcgcta acagaaactt catgcagctc
     4681 atccacgatg attctctcac ctttaaagag gatatccaga aggctcaggt ttcaggacag
     4741 ggtgatagtc tccatgagca tatcgctaac ctcgctggat ctcctgcaat caagaaggga
     4801 atcctccaga ctgtgaaggt tgtggatgag ttggtgaagg tgatgggaag gcataagcct
     4861 gagaacatcg tgatcgaaat ggctagagag aaccagacca ctcagaaggg acagaagaac
     4921 tctagggaaa ggatgaagag gatcgaggaa ggtatcaaag agcttggatc tcagatcctc
     4981 aaagagcacc ctgttgagaa cactcagctc cagaatgaga agctctacct ctactacctc
     5041 cagaacggaa gggatatgta tgtggatcaa gagttggata tcaacaggct ctctgattac
     5101 gatgttgatc atatcgtgcc acagtcattc ttgaaggatg attctatcga taacaaggtg
     5161 ctcaccaggt ctgataagaa caggggtaag agtgataacg tgccaagtga agaggttgtg
     5221 aagaaaatga agaactattg gaggcagctc ctcaacgcta agctcatcac tcagagaaag
     5281 ttcgataact tgactaaggc tgagagggga ggactctctg aattggataa ggcaggattc
     5341 atcaagaggc agcttgtgga aaccaggcag atcactaagc acgttgcaca gatcctcgat
     5401 tctaggatga acaccaagta cgatgagaac gataagttga tcagggaagt gaaggttatc
     5461 accctcaagt caaagctcgt gtctgatttc agaaaggatt tccaattcta caaggtgagg
     5521 gaaatcaaca actaccacca cgctcacgat gcttacctta acgctgttgt tggaaccgct
     5581 ctcatcaaga agtatcctaa gctcgagtca gagttcgtgt acggtgatta caaggtgtac
     5641 gatgtgagga agatgatcgc taagtctgag caagagatcg gaaaggctac cgctaagtat
     5701 ttcttctact ctaacatcat gaatttcttc aagaccgaga ttaccctcgc taacggtgag
     5761 atcagaaaga ggccactcat cgagacaaac ggtgaaacag gtgagatcgt gtgggataag
     5821 ggaagggatt tcgctaccgt tagaaaggtg ctctctatgc cacaggtgaa catcgttaag
     5881 aaaaccgagg tgcagaccgg tggattctct aaagagtcta tcctccctaa gaggaactct
     5941 gataagctca ttgctaggaa gaaggattgg gaccctaaga aatacggtgg tttcgattct
     6001 cctaccgtgg cttactctgt tctcgttgtg gctaaggttg agaagggaaa gagtaagaag
     6061 ctcaagtctg ttaaggaact tctcggaatc actatcatgg aaaggtcatc tttcgagaag
     6121 aacccaatcg atttcctcga ggctaaggga tacaaagagg ttaagaagga tctcatcatc
     6181 aagctcccaa agtactcact cttcgaactc gagaacggta gaaagaggat gctcgcttct
     6241 gctggtgagc ttcaaaaggg aaacgagctt gctctcccat ctaagtacgt taactttctt
     6301 tacctcgctt ctcactacga gaagttgaag ggatctccag aagataacga gcagaagcaa
     6361 cttttcgttg agcagcacaa gcactacttg gatgagatca tcgagcagat ctctgagttc
     6421 tctaaaaggg tgatcctcgc tgatgcaaac ctcgataagg tgttgtctgc ttacaacaag
     6481 cacagagata agcctatcag ggaacaggca gagaacatca tccatctctt cacccttacc
     6541 aacctcggtg ctcctgctgc tttcaagtac ttcgatacaa ccatcgatag gaagagatac
     6601 acctctacca aagaagtgct cgatgctacc ctcatccatc agtctatcac tggactctac
     6661 gagactagga tcgatctctc acagctcggt ggtgattcaa gggctgatcc taagaagaag
     6721 aggaaggttt gagcttgagc tcgaatttcc ccgatcgttc aaacatttgg caataaagtt
     6781 tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt
     6841 acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta
     6901 tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa
     6961 actaggataa attatcgcgc gcggtgtcat ctatgttact agatcgatcc gtatcgatag
     7021 cctctagcta gagtcgatcg acaagctcga gtttctccat aataatgtgt gagtagttcc
     7081 cagataaggg aattagggtt cctatagggt ttcgctcatg tgttgagcat ataagaaacc
     7141 cttagtatgt atttgtattt gtaaaatact tctatcaata aaatttctaa ttcctaaaat
     7201 caaaatccag tactaaaatc cagatcgcta ggtatacttg agaccggatc ctgacaggat
     7261 atattggcgg gtaaacctaa gagaaaagag cgtttattag aataatcgga tatttaaaag
     7321 ggcgtgaaaa ggtttatccg ttcgtccatt tgtatgtgca tgccaaccac agggttcccc
     7381 tcgggatcaa agtactttga tccaacccct ccgctgctat agtgcagtcg gcttctgacg
     7441 ttcagtgcag ccgtcatctg aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc
     7501 tgccgccctg cccttttcct ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa
     7561 tacttgcgac tagaaccgga gacattacgc catgaacaag agcgccgccg ctggcctgct
     7621 gggctatgcc cgcgtcagca ccgacgacca ggacttgacc aaccaacggg ccgaactgca
     7681 cgcggccggc tgcaccaagc tgttttccga gaagatcacc ggcaccaggc gcgaccgccc
     7741 ggagctggcc aggatgcttg accacctacg ccctggcgac gttgtgacag tgaccaggct
     7801 agaccgcctg gcccgcagca cccgcgacct actggacatt gccgagcgca tccaggaggc
     7861 cggcgcgggc ctgcgtagcc tggcagagcc gtgggccgac accaccacgc cggccggccg
     7921 catggtgttg accgtgttcg ccggcattgc cgagttcgag cgttccctaa tcatcgaccg
     7981 cacccggagc gggcgcgagg ccgccaaggc ccgaggcgtg aagtttggcc cccgccctac
     8041 cctcaccccg gcacagatcg cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt
     8101 gaaagaggcg gctgcactgc ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg
     8161 cagcgaggaa gtgacgccca ccgaggccag gcggcgcggt gccttccgtg aggacgcatt
     8221 gaccgaggcc gacgccctgg cggccgccga gaatgaacgc caagaggaac aagcatgaaa
     8281 ccgcaccagg acggccagga cgaaccgttt ttcattaccg aagagatcga ggcggagatg
     8341 atcgcggccg ggtacgtgtt cgagccgccc gcgcacctct caaccgtgcg gctgcatgaa
     8401 atcctggccg gtttgtctga tgccaagctg gcggcctggc cggccagctt ggccgctgaa
     8461 gaaaccgagc gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat
     8521 gcggtcgctg cgtatatgat gcgatgagta aataaacaaa tacgcaaggg gaacgcatga
     8581 aggttatcgc tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc
     8641 tagcccgcgc cctgcaactc gccggggccg atgttctgtt agtcgattcc gatccccagg
     8701 gcagtgcccg cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg
     8761 accgcccgac gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg
     8821 acggagcgcc ccaggcggcg gacttggctg tgtccgcgat caaggcagcc gacttcgtgc
     8881 tgattccggt gcagccaagc ccttacgaca tatgggccac cgccgacctg gtggagctgg
     8941 ttaagcagcg cattgaggtc acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg
     9001 cgatcaaagg cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg tacgagctgc
     9061 ccattcttga gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc gccgccggca
     9121 caaccgttct tgaatcagaa cccgagggcg acgctgcccg cgaggtccag gcgctggccg
     9181 ctgaaattaa atcaaaactc atttgagtta atgaggtaaa gagaaaatga gcaaaagcac
     9241 aaacacgcta agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa cgttggccag
     9301 cctggcagac acgccagcca tgaagcgggt caactttcag ttgccggcgg aggatcacac
     9361 caagctgaag atgtacgcgg tacgccaagg caagaccatt accgagctgc tatctgaata
     9421 catcgcgcag ctaccagagt aaatgagcaa atgaataaat gagtagatga attttagcgg
     9481 ctaaaggagg cggcatggaa aatcaagaac aaccaggcac cgacgccgtg gaatgcccca
     9541 tgtgtggagg aacgggcggt tggccaggcg taagcggctg ggttgcctgc cggccctgca
     9601 atggcactgg aacccccaag cccgaggaat cggcgtgagc ggtcgcaaac catccggccc
     9661 ggtacaaatc ggcgcggcgc tgggtgatga cctggtggag aagttgaagg ccgcgcaggc
     9721 cgcccagcgg caacgcatcg aggcagaagc acgccccggt gaatcgtggc aagcggccgc
     9781 tgatcgaatc cgcaaagaat cccggcaacc gccggcagcc ggtgcgccgt cgattaggaa
     9841 gccgcccaag ggcgacgagc aaccagattt tttcgttccg atgctctatg acgtgggcac
     9901 ccgcgatagt cgcagcatca tggacgtggc cgttttccgt ctgtcgaagc gtgaccgacg
     9961 agctggcgag gtgatccgct acgagcttcc agacgggcac gtagaggttt ccgcagggcc
    10021 ggccggcatg gccagtgtgt gggattacga cctggtactg atggcggttt cccatctaac
    10081 cgaatccatg aaccgatacc gggaagggaa gggagacaag cccggccgcg tgttccgtcc
    10141 acacgttgcg gacgtactca agttctgccg gcgagccgat ggcggaaagc agaaagacga
    10201 cctggtagaa acctgcattc ggttaaacac cacgcacgtt gccatgcagc gtacgaagaa
    10261 ggccaagaac ggccgcctgg tgacggtatc cgagggtgaa gccttgatta gccgctacaa
    10321 gatcgtaaag agcgaaaccg ggcggccgga gtacatcgag atcgagctag ctgattggat
    10381 gtaccgcgag atcacagaag gcaagaaccc ggacgtgctg acggttcacc ccgattactt
    10441 tttgatcgat cccggcatcg gccgttttct ctaccgcctg gcacgccgcg ccgcaggcaa
    10501 ggcagaagcc agatggttgt tcaagacgat ctacgaacgc agtggcagcg ccggagagtt
    10561 caagaagttc tgtttcaccg tgcgcaagct gatcgggtca aatgacctgc cggagtacga
    10621 tttgaaggag gaggcggggc aggctggccc gatcctagtc atgcgctacc gcaacctgat
    10681 cgagggcgaa gcatccgccg gttcctaatg tacggagcag atgctagggc aaattgccct
    10741 agcaggggaa aaaggtcgaa aaggactctt tcctgtggat agcacgtaca ttgggaaccc
    10801 aaagccgtac attgggaacc ggaacccgta cattgggaac ccaaagccgt acattgggaa
    10861 ccggtcacac atgtaagtga ctgatataaa agagaaaaaa ggcgattttt ccgcctaaaa
    10921 ctctttaaaa cttattaaaa ctcttaaaac ccgcctggcc tgtgcataac tgtctggcca
    10981 gcgcacagcc gaagtgctgc aaaaagcgcc tacccttcgg tcgctgcgct ccctacgccc
    11041 cgccgcttcg cgtcggccta tcgcggccgc tggccgctca aaaatggctg gcctacggcc
    11101 aggcaatcta ccagggcgcg gacaagccgc gccgtcgcca ctcgaccgcc ggcgcccaca
    11161 tcaaggcacc ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
    11221 tcccggtgac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
    11281 gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata
    11341 gcggagtgta tactggctta actatgcggc atcagagcag attgtactga gagtgcacca
    11401 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcatc
    11461 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
    11521 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
    11581 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
    11641 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
    11701 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
    11761 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
    11821 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
    11881 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
    11941 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
    12001 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
    12061 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
    12121 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
    12181 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
    12241 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
    12301 gcattctagg tgattatttg ccgactacct tggtgatctc gcctttcacg tagtggacaa
    12361 attcttccaa ctgatctgcg cgcgaggcca agcgatcttc ttcttgtcca agataagcct
    12421 gtctagcttc aagtatgacg ggctgatact gggccggcag gcgctccatt gcccagtcgg
    12481 cagcgacatc cttcggcgcg attttgccgg ttactgcgct gtaccaaatg cgggacaacg
    12541 taagcactac atttcgctca tcaccagccc agtcgggcgg cgagttccat agcgttaagg
    12601 tttcatttag cgcctcaaat agatcctgtt caggaaccgg atcaaagagt tcctccgccg
    12661 ctggacctac caaggcaacg ctatgttctc ttgcttttgt cagcaagata gccagatcaa
    12721 tgtcgatcgt ggctggctcg aagatacctg caagaatgtc attgcgctgc cattctccaa
    12781 attgcagttc gcgcttagct ggataacgcc acggaatgat gtcgtcgtgc acaacaatgg
    12841 tgacttctac agcgcggaga atctcgctct ctccagggga agccgaagtt tccaaaaggt
    12901 cgttgatcaa agctcgccgc gttgtttcat caagccttac ggtcaccgta accagcaaat
    12961 caatatcact gtgtggcttc aggccgccat ccactgcgga gccgtacaaa tgtacggcca
    13021 gcaacgtcgg ttcgagatgg cgctcgatga cgccaactac ctctgatagt tgagtcgata
    13081 cttcggcgat caccgcttcc ctcataatgt ttaactttgt tttagggcga ctgccctgct
    13141 gcgtaacatc gttgctgctc cataacatca aacatcgacc cacggcgtaa cgcgcttgct
    13201 gcttggatgc ccgaggcata gactgtaccc caaaaaaaca gtcataacaa gccatgaaaa
    13261 ccgccactgc gccgttacca ccgctgcgtt cggtcaaggt tctggaccag ttgcgtgagc
    13321 gcatacgcta cttgcattac agcttacgaa ccgaacaggc ttatgtccac tgggttcgtg
    13381 ccttcatccg tttccacggt gtgcgtcacc cggcaacctt gggtagcagc gaagtcgagg
    13441 catttctgtc ctggctggaa cagaacttat tatttccttc ctcttttcta cagtatttaa
    13501 agatacccca agaagctaat tataacaaga cgaactccaa ttcactgttc cttgcattct
    13561 aaaaccttaa ataccagaaa acagcttttt caaagttgtt ttcaaagttg gcgtataaca
    13621 tagtatcgac ggagccgatt ttgaaaccgc ggtgatcaca ggcagcaacg ctctgtcatc
    13681 gttacaatca acatgctacc ctccgcgaga tcatccgtgt ttcaaacccg gcagcttagt
    13741 tgccgttctt ccgaatagca tcggtaacat gagcaaagtc tgccgcctta caacggctct
    13801 cccgctgacg ccgtcccgga ctgatgggct gcctgtatcg agtggtgatt ttgtgccgag
    13861 ctgccggtcg gggagctgtt ggctggctgg tggcaggata tattgtggtg taaacataac
    13921 ggatccggtc tcaggagaat gaggaggcta gagcagcttg ccaacatggt ggagcacgac
    13981 actctcgtct actccaagaa tatcaaagat acagtctcag aagaccaaag ggctattgag
    14041 acttttcaac aaagggtaat atcgggaaac ctcctcggat tccattgccc agctatctgt
    14101 cacttcatca aaaggacagt agaaaaggaa ggtggcacct acaaatgcca tcattgcgat
    14161 aaaggaaagg ctatcgttca agatgcctct gccgacagtg gtcccaaaga tggaccccca
    14221 cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat
    14281 tgatgtgaac atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt
    14341 ctcagaagac caaagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct
    14401 cggattccat tgcccagcta tctgtcactt catcaaaagg acagtagaaa aggaaggtgg
    14461 cacctacaaa tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctgccga
    14521 cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc
    14581 aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc
    14641 acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga
    14701 gaggacacgc tgaaatcacc agtctctctc tacaaatcta tctctctcga gctttcgcag
    14761 atccgggggg caatgagatt caccaatgaa aaagcctgaa ctcaccgcga cgtctgtcga
    14821 gaagtttctg atcgaaaagt tcgacagcgt ctccgacctg atgcagctct cggagggcga
    14881 agaatctcgt gctttcagct tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag
    14941 ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg cactttgcat cggccgcgct
    15001 cccgattccg gaagtgcttg acattgggga gtttagcgag agcctgacct attgcatctc
    15061 ccgccgttca cagggtgtca cgttgcaaga cctgcctgaa accgaactgc ccgctgttct
    15121 acaaccggtc gcggaggcta tggatgcgat cgctgcggcc gatcttagcc agacgagcgg
    15181 gttcggccca ttcggaccgc aaggaatcgg tcaatacact acatggcgtg atttcatatg
    15241 cgcgattgct gatccccatg tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc
    15301 gtccgtcgcg caggctctcg atgagctgat gctttgggcc gaggactgcc ccgaagtccg
    15361 gcacctcgtg cacgcggatt tcggctccaa caatgtcctg acggacaatg gccgcataac
    15421 agcggtcatt gactggagcg aggcgatgtt cggggattcc caatacgagg tcgccaacat
    15481 cttcttctgg aggccgtggt tggcttgtat ggagcagcag acgcgctact tcgagcggag
    15541 gcatccggag cttgcaggat cgccacgact ccgggcgtat atgctccgca ttggtcttga
    15601 ccaactctat cagagcttgg ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg
    15661 atgcgacgca atcgtccgat ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag
    15721 aagcgcggcc gtctggaccg atggctgtgt agaagtactc gccgatagtg gaaaccgacg
    15781 ccccagcact cgtccgaggg caaagaaata ggcttctcta gctagagtcg atcgacaagc
    15841 tcgagtttct ccataataat gtgtgagtag ttcccagata agggaattag ggttcctata
    15901 gggtttcgct catgtgttga gcatataaga aacccttagt atgtatttgt atttgtaaaa
    15961 tacttctatc aataaaattt ctaattccta aaaccaaaat ccagtactaa aatccagatc
    16021 gctagcaagg agtctgagca ggacaactcg cgtagtgaga gttacatgtt cgttgggttc
    16081 ttccgacacg gacctgagtt ggccaacgtc ccacctgagg tctgtgcccc ggtgatgaga
    16141 agtgtgcatc tcgttcttgc agctcgtcag tactttcaga atcatggcgt gcatggtaga
    16201 atgaccctta taacggactt cgacatggca atcgctatac aggagtattc attcaaaaga
    16261 gatttttaaa gatcaatttt tctttctcat aagtctaata cgtcatgata ataactttaa
    16321 aaaaaattga actcgttaaa aaatttgatc aaattttaat ttattattag atcatatatt
    16381 acatatcagt ttcataatgt tatactaaga tctttcatat atattttaac ccacgagaca
    16441 aaatatgttt atatctttat ttatttaaat attttttagc tcaaaacatc gcaaactcag
    16501 ctatcataat gctttttaat gaaaaaaaca aattttccta tcatactctc attgtttata
    16561 attttttaga aaaaaaactt gaagataaaa taacaacaat aatagtttca aaagcacatt
    16621 tttgttgtaa atttcaagaa aaatacaatc atattactga ttgactacaa aaatttgtaa
    16681 attaatccag gctacatgtt taagatgtgt caaaactgca tcatctacat ctcattttaa
    16741 tttttctaaa ctacatacat agtatcaata acaattgaac aatgctagtt aatgtgttat
    16801 actcattagc ttgtaatata tcttcaactt gatgaatcgg attgatcata aaatttcaca
    16861 aacttatgtg ctgattaata aaaaatataa tcagattttt catgaataat taagataaat
    16921 aacacatgca agtctcaatt agaatttaac aatctgacta taaaaagtcg gtttatcttg
    16981 attttcacaa aaatttaaca tagtttcaag gtgaatgggc ttttatttta atccaatcaa
    17041 ctatttgaca catacatctt gcaaatttat ttatttttta tggaaaaaaa catgatttat
    17101 gtttttcttt gagaaagtat attgtggatt gtcaaaaaaa acctatggaa gatttatatg
    17161 taagagttaa aaatttattt tcaaatatgt caacaatcca caatatcgaa gaaaattttc
    17221 aaaaaatcat cacatatgat gtaaatataa tatgaatatg tgcaatctta tttcatttta
    17281 ctctgaaaac ttaatttctt tttatagagc attctgtact tactggtgtc aaatatacag
    17341 ataaaatgca gtccataacg tatcactcta taagcaaaat cataaacttc gtgatctctc
    17401 acagttcaaa cgagaactat agaaaccctt caactagcaa aaaactcaca actttctatt
    17461 ccaatatctt taattgtctg acgatttaca aatagaaacg aaaaccagac acacacacac
    17521 acgcaaacta caaagtaaaa acacaaccac ctgacattag acagtgagat tattaaacat
    17581 cctaaactaa aaaacaaaca aattaattct ctatgtacaa gtccccatcg tgatcaattt
    17641 ttgcatccca atatagtggc ggttagtata agaggacctt cgtctattca ctctccatgt
    17701 ttgtcaaact atagtacagt acatatatgt atgggaacaa attccgaaca ctctagtaca
    17761 ctcctcaacg caagttctat ctcatatctc tgcaagagct tattccaaga aactttcttg
    17821 tgcttctatg catgcccagg taccttaagt cgcgcgcata gtcacttcac agccttttca
    17881 gttctccctt tgatggctca gagtgacgca actgcacaaa aataagaaaa aagtcgaagc
    17941 gtcacgagag atgtatccgg ctagtttcct tggagttcgg attgctctct ttctctgtct
    18001 ccccgaacca tatggaattt atgaactgct gttcactagt cagtcacttg tttcagaact
    18061 tttgagtggt cgatttatcc accttcttgt ttcttcctgg gtccagaatc tgacttc
//
