LOCUS       sequence-275278-       14899 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Pstabilized - TARGET8 - MevT - MBIS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14899)
  AUTHORS   Glasscock CJ, Biggs BW, Lazar JT, Arnold JH, Burdette LA, Valdes A, 
            Kang MK, Tullman-Ercek D, Tyo KEJ, Lucks JB
  TITLE     Dynamic Control of Gene Expression with Riboregulated Switchable 
            Feedback Promoters.
  JOURNAL   ACS Synth Biol. 2021 May 21;10(5):1199-1213. doi: 
            10.1021/acssynbio.1c00015. Epub 2021 Apr 9.
  PUBMED    33834762
REFERENCE   2  (bases 1 to 14899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00015"; journalName: "ACS Synth Biol"; date: 
            "2021-05-21- 21"; volume: "10"; issue: "5"; pages: "1199-1213"
COMMENT     Sequence Label: pJBL6731
FEATURES             Location/Qualifiers
     source          1..14899
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..19
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(23..66)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    69..90
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     CDS             complement(3956..3967)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             6880..6891
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             10498..10509
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      12923..12994
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      13010..13037
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      13076..13147
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      13163..13190
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(13218..13235)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13352..13897)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(13432..13451)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(14011..14105)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(14126..14785)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     14700..14719
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(14786..14888)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
ORIGIN
        1 aataggcgta tcacgaggca gaatttcaga taaaaaaaat ccttagcttt cgctaaggat
       61 gatttctgga attcgcggcc gcttctagag gaagacacgg agctcggtac caaattccag
      121 aaaagaggcc tcccgaaagg ggggcctttt ttcgttttgg tcctactggc gcgcctcagt
      181 cagagtattg acttaaagtc taacctatag gaccgttaca gccatcgaga gctgcgagac
      241 tgtcgccgga tgtgtatccg acctgacgat ggcccaaaag ggccgaaaca gtcctctaca
      301 aataattttg tttaaatcaa ttcacctgcg tgaaaatggt agatttaaga actttaggat
      361 attcacagca gcaacaggaa aagatcaagc ccaaagttag gtcgacagtc gcgcagcatc
      421 acgaagcgct ggttggtcat gggtttacac atgcccacat cgtagcctta tcgcagcacc
      481 ctgcagccct tggcacggtc gccgtcaagt accaggacat gattgcggcg ttgccggaag
      541 ccacacatga ggcgatcgtc ggtgtgggga aacagtggag cggagcccga gcgcttgagg
      601 ccctgttgac ggtcgcggga gagctgagag ggcctcccct tcagctggac acgggccagt
      661 tgctgaagat cgcgaagcgg ggaggagtca cggcggtcga ggcggtgcac gcgtggcgca
      721 atgcgctcac gggagcaccc ctcaatctga ccccggatca ggtggttgcg atcgccagta
      781 atggcggagg caaacaagca ctcgaaactg tacagcgcct cctgccggta ctgtgccaag
      841 atcatggctt gacgcctgag caagtcgtag ctattgcatc aaacatcggt ggtaaacagg
      901 cgctggaaac cgtacaacga ttactccctg tcttatgcca ggcacacggt ctgacccccg
      961 accaagttgt agccattgcg tctaatggcg gcgggaaaca ggccctggaa acggtccaac
     1021 gtctgttacc cgttctgtgt caggctcacg gtctgacccc tgcccaggta gttgcaattg
     1081 ccagcaacat cggcgggaaa caagcgctgg agactgtgca gcgtctgctc cctgtgttat
     1141 gccaagatca tgggctcact ccggatcagg tggtggccat cgcttccaat attggcggta
     1201 aacaggcgct ggagacagtg caacgacttt tacctgttct ctgccaggat catggtctaa
     1261 ctcccgagca ggtcgtcgcc atcgcctctc atgacggcgg gaaacaagcg ttggaaactg
     1321 tccagcgact cctgccggtt ttgtgccagg cccacgggct tactcctgac caggtagttg
     1381 cgatcgcgtc aaatgggggt ggcaaacaag ccctcgaaac cgtgcaacgc ctgctgcccg
     1441 tcttgtgcca agctcatggg ctgactccgg cgcaagtagt cgcgattgcg agccacgatg
     1501 gcggtaagca ggcactggaa acggttcagc gcctgctccc ggttctatgc caggatcacg
     1561 gcctgacccc ggaccaggtc gtcgcgatcg cgtcaaatat cggtggcaaa caagctttgg
     1621 agacagtaca gcgcctgtta ccagtgcttt gccaggacca tggtctgacc cctgagcaag
     1681 tagtggcgat cgcttctaat attgggggca aacaagcgct ggaaacagta cagcgtctgt
     1741 taccggtcct atgccaggca catggcctga cccctgatca ggtggtagcc attgccagtc
     1801 atgatggcgg taaacaggcg cttgagactg tccaacgtct gctgccggtc ctctgtcagg
     1861 ctcatggcct gacgccagct caagtcgtgg ctatcgcttc gcatgatggc ggaaaacagg
     1921 cactggagac tgtgcagcga ctgttgccag ttctgtgtca ggatcacggt ttaactccgg
     1981 accaggtggt cgctattgcg tcgaatgggg gcggtaaaca agcgctggaa actgtgcaac
     2041 gtttgctccc agttctgtgc caggaccatg ggctgactcc ggaacaggta gtggccattg
     2101 cttctaatat tggtgggaaa caggcgctgg aaaccgtgca gcgcctgctt ccagtgcttt
     2161 gccaggccca tggcctgacg ccagatcagg tggttgctat agccagcaat ggcggcggta
     2221 aacaggccct cgaaaccgtc cagcgcctgc tccctgtgct gtgccaggcc catgggctta
     2281 ccccagcgca agtagtggcg attgcgtcta atattggtgg taaacaggcg ttggagactg
     2341 tacaacgcct gctgccagtt ttatgccaag atcatggtct gacccctgag caggtagtgg
     2401 ctattgcatc caacaacggg ggcagacccg cactggagtc aatcgtggcc cagctttcga
     2461 ggccggaccc cgcgctggcc gcactcacta atgatcatct tgtagcgctg gcctgcctcg
     2521 gcggacgacc cgccttggat gcggtgaaga aggggctccc gcacgcgcct gcattgatta
     2581 agcggaccaa cagaaggatt cccgagagga catcacatcg agtggcagat cacgcgcaag
     2641 tggtccgcgt gctcggattc ttccagtgtc actcccaccc cgcacaagcg ttcgatgacg
     2701 ccatgactca atttggtatg tcgagacacg gactgctgca gctctttcgt agagtcggtg
     2761 tcacagaact cgaggcccgc tcgggcacac tgcctcccgc ctcccagcgg tgggacagga
     2821 ttctccaagc gagcggtatg aaacgcgcga agccttcacc tacgtcaact cagacacctg
     2881 accaggcgag ccttcatgcg ttcgcagact cgctggagag ggatttggac gcgccctcgc
     2941 ccatgcatga aggggaccaa actcgcgcgt cataataggt ttcagccaaa aaacttaaga
     3001 ccgccggtct tgtccactac cttgcagtaa tgcggtggac aggatcggcg gttttctttt
     3061 ctcttctcaa gcttatcccg aaaatttatc aaaaagagta ttgacttata actcaaccta
     3121 taggatactt acagccatcg agagctgcgc catcctcaat ctctacctac tctcacttac
     3181 tcttatcctg cggggaatgt atacagttca tgtatatatt ccccgctttt tttttggatc
     3241 ttaggaggaa tataaaatga aaaattgtgt catcgtcagt gcggtacgta ctgctatcgg
     3301 tagttttaac ggttcactcg cttccaccag cgccatcgac ctgggggcga cagtaattaa
     3361 agccgccatt gaacgtgcaa aaatcgattc acaacacgtt gatgaagtga ttatgggtaa
     3421 cgtgttacaa gccgggctgg ggcaaaatcc ggcgcgtcag gcactgttaa aaagcgggct
     3481 ggcagaaacg gtgtgcggat tcacggtcaa taaagtatgt ggttcgggtc ttaaaagtgt
     3541 ggcgcttgcc gcccaggcca ttcaggcagg tcaggcgcag agcattgtgg cggggggtat
     3601 ggaaaatatg agtttagccc cctacttact cgatgcaaaa gcacgctctg gttatcgtct
     3661 tggagacgga caggtttatg acgtaatcct gcgcgatggc ctgatgtgcg ccacccatgg
     3721 ttatcatatg gggattaccg ccgaaaacgt ggctaaagag tacggaatta cccgtgaaat
     3781 gcaggatgaa ctggcgctac attcacagcg taaagcggca gccgcaattg agtccggtgc
     3841 ttttacagcc gaaatcgtcc cggtaaatgt tgtcactcga aagaaaacct tcgtcttcag
     3901 tcaagacgag ttcccgaaag cgaactcaac ggctgaagcg ttaggtgcat tgcgcccggc
     3961 cttcgataaa gcaggaacag tcaccgctgg gaacgcgtct ggtattaacg acggtgctgc
     4021 cgctctggtg attatggaag aatctgcggc gctggcagca ggccttaccc ccctggctcg
     4081 cattaaaagt tatgccagcg gtggcgtgcc ccccgcattg atgggtatgg ggccagtacc
     4141 tgccacgcaa aaagcgttac aactggcggg gctgcaactg gcggatattg atctcattga
     4201 ggctaatgaa gcatttgctg cacagttcct tgccgttggg aaaaacctgg gctttgattc
     4261 tgagaaagtg aatgtcaacg gcggggccat cgcgctcggg catcctatcg gtgccagtgg
     4321 tgctcgtatt ctggtcacac tattacatgc catgcaggca cgcgataaaa cgctggggct
     4381 ggcaacactg tgcattggcg gcggtcaggg aattgcgatg gtgattgaac ggttgaattg
     4441 aggatcttga attaaggagg acagctaaat gaaactctca actaaacttt gttggtgtgg
     4501 tattaaagga agacttaggc cgcaaaagca acaacaatta cacaatacaa acttgcaaat
     4561 gactgaacta aaaaaacaaa agaccgctga acaaaaaacc agacctcaaa atgtcggtat
     4621 taaaggtatc caaatttaca tcccaactca atgtgtcaac caatctgagc tagagaaatt
     4681 tgatggcgtt tctcaaggta aatacacaat tggtctgggc caaaccaaca tgtcttttgt
     4741 caatgacaga gaagatatct actcgatgtc cctaactgtt ttgtctaagt tgatcaagag
     4801 ttacaacatc gacaccaaca aaattggtag attagaagtc ggtactgaaa ctctgattga
     4861 caagtccaag tctgtcaagt ctgtcttgat gcaattgttt ggtgaaaaca ctgacgtcga
     4921 aggtattgac acgcttaatg cctgttacgg tggtaccaac gcgttgttca actctttgaa
     4981 ctggattgaa tctaacgcat gggatggtag agacgccatt gtagtttgcg gtgatattgc
     5041 catctacgat aagggtgccg caagaccaac cggtggtgcc ggtactgttg ctatgtggat
     5101 cggtcctgat gctccaattg tatttgactc tgtaagagct tcttacatgg aacacgccta
     5161 cgatttttac aagccagatt tcaccagcga atatccttac gtcgatggtc atttttcatt
     5221 aacttgttac gtcaaggctc ttgatcaagt ttacaagagt tattccaaga aggctatttc
     5281 taaagggttg gttagcgatc ccgctggttc ggatgctttg aacgttttga aatatttcga
     5341 ctacaacgtt ttccatgttc caacctgtaa attggtcaca aaatcatacg gtagattact
     5401 atataacgat ttcagagcca atcctcaatt gttcccagaa gttgacgccg aattagctac
     5461 tcgcgattat gacgaatctt taaccgataa gaacattgaa aaaacttttg ttaatgttgc
     5521 taagccattc cacaaagaga gagttgccca atctttgatt gttccaacaa acacaggtaa
     5581 catgtacacc gcatctgttt atgccgcctt tgcatctcta ttaaactatg ttggatctga
     5641 cgacttacaa ggcaagcgtg ttggtttatt ttcttacggt tccggtttag ctgcatctct
     5701 atattcttgc aaaattgttg gtgacgtcca acatattatc aaggaattag atattactaa
     5761 caaattagcc aagagaatca ccgaaactcc aaaggattac gaagctgcca tcgaattgag
     5821 agaaaatgcc catttgaaga agaacttcaa acctcaaggt tccattgagc atttgcaaag
     5881 tggtgtttac tacttgacca acatcgatga caaatttaga agatcctacg atgttaaaaa
     5941 atgaggatct aaaataagga ggattacact atggttttaa ccaataaaac agtcatttct
     6001 ggatcgaaag tcaaaagttt atcatctgcg caatcgagct catcaggacc ttcatcatct
     6061 agtgaggaag atgattcccg cgatattgaa agcttggata agaaaatacg tcctttagaa
     6121 gaattagaag cattattaag tagtggaaat acaaaacaat tgaagaacaa agaggtcgct
     6181 gccttggtta ttcacggtaa gttacctttg tacgctttgg agaaaaaatt aggtgatact
     6241 acgagagcgg ttgcggtacg taggaaggct ctttcaattt tggcagaagc tcctgtatta
     6301 gcatctgatc gtttaccata taaaaattat gactacgacc gcgtatttgg cgcttgttgt
     6361 gaaaatgtta taggttacat gcctttgccc gttggtgtta taggcccctt ggttatcgat
     6421 ggtacatctt atcatatacc aatggcaact acagagggtt gtttggtagc ttctgccatg
     6481 cgtggctgta aggcaatcaa tgctggcggt ggtgcaacaa ctgttttaac taaggatggt
     6541 atgacaagag gcccagtagt ccgtttccca actttgaaaa gatccggtgc ctgtaagata
     6601 tggttagact cagaagaggg acaaaacgca attaaaaaag cttttaactc tacatcaaga
     6661 tttgcacgtc tgcaacatat tcaaacttgt ctagcaggag atttactctt catgagattt
     6721 agaacaacta ctggtgacgc aatgggtatg aatatgattt ctaaaggtgt cgaatactca
     6781 ttaaagcaaa tggtagaaga gtatggctgg gaagatatgg aggttgtctc cgtttctggt
     6841 aactactgta ccgacaaaaa accagctgcc atcaactgga tcgaaggtcg tggtaagagt
     6901 gtcgtcgcag aagctactat tcctggtgat gttgtcagaa aagtgttaaa aagtgatgtt
     6961 tccgcattgg ttgagttgaa cattgctaag aatttggttg gatctgcaat ggctgggtct
     7021 gttggtggat ttaacgcaca tgcagctaat ttagtgacag ctgttttctt ggcattagga
     7081 caagatcctg cacaaaatgt tgaaagttcc aactgtataa cattgatgaa agaagtggac
     7141 ggtgatttga gaatttccgt atccatgcca tccatcgaag taggtaccat cggtggtggt
     7201 actgttctag aaccacaagg tgccatgttg gacttattag gtgtaagagg cccgcatgct
     7261 accgctcctg gtaccaacgc acgtcaatta gcaagaatag ttgcctgtgc cgtcttggca
     7321 ggtgaattat ccttatgtgc tgccctagca gccggccatt tggttcaaag tcatatgacc
     7381 cacaacagga aacctgctga accaacaaaa cctaacaatt tggacgccac tgatataaat
     7441 cgtttgaaag atgggtccgt cacctgcatt aaatcctgag gatctaggag gttaattgga
     7501 tgtcattacc gttcttaact tctgcaccgg gaaaggttat tatttttggt gaacactctg
     7561 ctgtgtacaa caagcctgcc gtcgctgcta gtgtgtctgc gttgagaacc tacctgctaa
     7621 taagcgagtc atctgcacca gatactattg aattggactt cccggacatt agctttaatc
     7681 ataagtggtc catcaatgat ttcaatgcca tcaccgagga tcaagtaaac tcccaaaaat
     7741 tggccaaggc tcaacaagcc accgatggct tgtctcagga actcgttagt cttttggacc
     7801 cgttgttagc tcaactatcc gaatccttcc actaccatgc agcgttttgt ttcctgtata
     7861 tgtttgtttg cctatgcccc catgccaaga atattaagtt ttctttaaag tctactttac
     7921 ccatcggtgc tgggttgggc tcaagcgcct ctatttctgt atcactggcc ttagctatgg
     7981 cctacttggg ggggttaata ggatctaatg acttggaaaa gctgtcagaa aacgataagc
     8041 atatagtgaa tcaatgggcc ttcataggtg aaaagtgtat tcacggtacc ccttcaggaa
     8101 tagataacgc tgtggccact tatggtaatg ccctgctatt tgaaaaagac tcacataatg
     8161 gaacaataaa cacaaacaat tttaagttct tagatgattt cccagccatt ccaatgatcc
     8221 taacctatac taggattcca aggtctacaa aagaccttgt tgctcgcgtt cgtgtgttgg
     8281 tcaccgagaa atttcctgaa gttatgaagc caattctaga tgccatgggt gaatgtgccc
     8341 tacaaggctt agagatcatg actaagttaa gtaaatgtaa aggcaccgat gacgaggctg
     8401 tagaaaataa taatgaactg tatgaacaac tattggaatt gataagaata aatcatggac
     8461 tgcttgtctc aatcggtgtt tctcatcctg gattagaact tattaaaaat ctgagcgatg
     8521 atttgagaat tggctccaca aaacttaccg gtgctggtgg cggcggttgc tctttgactt
     8581 tgttacgaag agacattact caagagcaaa ttgacagctt caaaaagaaa ttgcaagatg
     8641 attttagtta cgagacattt gaaacagact tgggtgggac tggctgctgt ttgttaagcg
     8701 caaaaaattt gaataaagac cttaaaatca aatccctagt attccaatta tttgaaaata
     8761 aaactaccac aaagcaacaa attgacgatc tattattgcc aggaaacacg aatttaccat
     8821 ggacttcata gggatcttaa ggaggatacc ctatgtcaga gttgagagcc ttcagtgccc
     8881 cagggaaagc gttactagct ggtggatatt tagttttaga tacaaaatat gaagcatttg
     8941 tagtcggatt atcggcaaga atgcatgctg tagcccatcc ttacggttca ttgcaagggt
     9001 ctgataagtt tgaagtgcgt gtgaaaagta aacaatttaa agatggggag tggctgtacc
     9061 atataagtcc taaaagtggc ttcattcctg tttcgatagg cggatctaag aaccctttca
     9121 ttgaaaaagt tatcgctaac gtatttagct actttaaacc taacatggac gactactgca
     9181 atagaaactt gttcgttatt gatattttct ctgatgatgc ctaccattct caggaggata
     9241 gcgttaccga acatcgtggc aacagaagat tgagttttca ttcgcacaga attgaagaag
     9301 ttcccaaaac agggctgggc tcctcggcag gtttagtcac agttttaact acagctttgg
     9361 cctccttttt tgtatcggac ctggaaaata atgtagacaa atatagagaa gttattcata
     9421 atttagcaca agttgctcat tgtcaagctc agggtaaaat tggaagcggg tttgatgtag
     9481 cggcggcagc atatggatct atcagatata gaagattccc acccgcatta atctctaatt
     9541 tgccagatat tggaagtgct acttacggca gtaaactggc gcatttggtt gatgaagaag
     9601 actggaatat tacgattaaa agtaaccatt taccttcggg attaacttta tggatgggcg
     9661 atattaagaa tggttcagaa acagtaaaac tggtccagaa ggtaaaaaat tggtatgatt
     9721 cgcatatgcc agaaagcttg aaaatatata cagaactcga tcatgcaaat tctagattta
     9781 tggatggact atctaaacta gatcgcttac acgagactca tgacgattac agcgatcaga
     9841 tatttgagtc tcttgagagg aatgactgta cctgtcaaaa gtatcctgaa atcacagaag
     9901 ttagagatgc agttgccaca attagacgtt cctttagaaa aataactaaa gaatctggtg
     9961 ccgatatcga acctcccgta caaactagct tattggatga ttgccagacc ttaaaaggag
    10021 ttcttacttg cttaatacct ggtgctggtg gttatgacgc cattgcagtg attactaagc
    10081 aagatgttga tcttagggct caaaccgcta atgacaaaag attttctaag gttcaatggc
    10141 tggatgtaac tcaggctgac tggggtgtta ggaaagaaaa agatccggaa acttatcttg
    10201 ataaataggg atctaggagg attatgagat gaccgtttac acagcatccg ttaccgcacc
    10261 cgtcaacatc gcaaccctta agtattgggg gaaaagggac acgaagttga atctgcccac
    10321 caattcgtcc atatcagtga ctttatcgca agatgacctc agaacgttga cctctgcggc
    10381 tactgcacct gagtttgaac gcgacacttt gtggttaaat ggagaaccac acagcatcga
    10441 caatgaaaga actcaaaatt gtctgcgcga cctacgccaa ttaagaaagg aaatggaatc
    10501 gaaggacgcc tcattgccca cattatctca atggaaactc cacattgtct ccgaaaataa
    10561 ctttcctaca gcagctggtt tagcttcctc cgctgctggc tttgctgcat tggtctctgc
    10621 aattgctaag ttataccaat taccacagtc aacttcagaa atatctagaa tagcaagaaa
    10681 ggggtctggt tcagcttgta gatcgttgtt tggcggatac gtggcctggg aaatgggaaa
    10741 agctgaagat ggtcatgatt ccatggcagt acaaatcgca gacagctctg actggcctca
    10801 gatgaaagct tgtgtcctag ttgtcagcga tattaaaaag gatgtgagtt ccactcaggg
    10861 tatgcaattg accgtggcaa cctccgaact atttaaagaa agaattgaac atgtcgtacc
    10921 aaagagattt gaagtcatgc gtaaagccat tgttgaaaaa gatttcgcca cctttgcaaa
    10981 ggaaacaatg atggattcca actctttcca tgccacatgt ttggactctt tccctccaat
    11041 attctacatg aatgacactt ccaagcgtat catcagttgg tgccacacca ttaatcagtt
    11101 ttacggagaa acaatcgttg catacacgtt tgatgcaggt ccaaatgctg tgttgtacta
    11161 cttagctgaa aatgagtcga aactctttgc atttatctat aaattgtttg gctctgttcc
    11221 tggatgggac aagaaattta ctactgagca gcttgaggct ttcaaccatc aatttgaatc
    11281 atctaacttt actgcacgtg aattggatct tgagttgcaa aaggatgttg ccagagtgat
    11341 tttaactcaa gtcggttcag gcccacaaga aacaaacgaa tctttgattg acgcaaagac
    11401 tggtctacca aaggaataag gatctaggag gtaatgataa tgcaaacgga acacgtcatt
    11461 ttattgaatg cacagggagt tcccacgggt acgctggaaa agtatgccgc acacacggca
    11521 gacacccgct tacatctcgc gttctccagt tggctgttta atgccaaagg acaattatta
    11581 gttacccgcc gcgcactgag caaaaaagca tggcctggcg tgtggactaa ctcggtttgt
    11641 gggcacccac aactgggaga aagcaacgaa gacgcagtga tccgccgttg ccgttatgag
    11701 cttggcgtgg aaattacgcc tcctgaatct atctatcctg actttcgcta ccgcgccacc
    11761 gatccgagtg gcattgtgga aaatgaagtg tgtccggtat ttgccgcacg caccactagt
    11821 gcgttacaga tcaatgatga tgaagtgatg gattatcaat ggtgtgattt agcagatgta
    11881 ttacacggta ttgatgccac gccgtgggcg ttcagtccgt ggatggtgat gcaggcgaca
    11941 aatcgcgaag ccagaaaacg attatctgca tttacccagc ttaaataagg atctaggagg
    12001 ttactcatat ggactttccg cagcaactcg aagcctgcgt taagcaggcc aaccaggcgc
    12061 tgagccgttt tatcgcccca ctgccctttc agaacactcc cgtggtcgaa accatgcagt
    12121 atggcgcatt attaggtggt aagcgcctgc gacctttcct ggtttatgcc accggtcata
    12181 tgttcggcgt tagcacaaac acgctggacg cacccgctgc cgccgttgag tgtatccacg
    12241 cttactcatt aattcatgat gatttaccgg caatggatga tgacgatctg cgtcgcggtt
    12301 tgccaacctg ccatgtgaag tttggcgaag caaacgcgat tctcgctggc gacgctttac
    12361 aaacgctggc gttctcgatt ttaagcgatg ccgatatgcc ggaagtgtcg gaccgcgaca
    12421 gaatttcgat gatttctgaa ctggcgagcg ccagtggtat tgccggaatg tgcggtggtc
    12481 aggcattaga tttagacgcg gaaggcaaac acgtacctct ggacgcgctt gagcgtattc
    12541 atcgtcataa aaccggcgca ttgattcgcg ccgccgttcg ccttggtgca ttaagcgccg
    12601 gagataaagg acgtcgtgct ctgccggtac tcgacaagta tgcagagagc atcggccttg
    12661 ccttccaggt tcaggatgac atcctggatg tggtgggaga tactgcaacg ttgggaaaac
    12721 gccagggtgc cgaccagcaa cttggtaaaa gtacctaccc tgcacttctg ggccttgagc
    12781 aagcccggaa gaaagcccgg gatctgatcg acgatgcccg tcagtcgctg aaacaactgg
    12841 ctgaacagtc actcgatacc tcggcactgg aagcgctagc ggactacatc atccagcgta
    12901 ataaataagg atctccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
    12961 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc
    13021 gggtgggcct ttctgcgttt ataggatcct aactcgagta aggatctcca ggcatcaaat
    13081 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
    13141 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttataccta
    13201 gggatatatt ccgcttcctc gctcactgac tcgctacgct cggtcgttcg actgcggcga
    13261 gcggaaatgg cttacgaacg gggcggagat ttcctggaag atgccaggaa gatacttaac
    13321 agggaagtga gagggccgcg gcaaagccgt ttttccatag gctccgcccc cctgacaagc
    13381 atcacgaaat ctgacgctca aatcagtggt ggcgaaaccc gacaggacta taaagatacc
    13441 aggcgtttcc ccctggcggc tccctcgtgc gctctcctgt tcctgccttt cggtttaccg
    13501 gtgtcattcc gctgttatgg ccgcgtttgt ctcattccac gcctgacact cagttccggg
    13561 taggcagttc gctccaagct ggactgtatg cacgaacccc ccgttcagtc cgaccgctgc
    13621 gccttatccg gtaactatcg tcttgagtcc aacccggaaa gacatgcaaa agcaccactg
    13681 gcagcagcca ctggtaattg atttagagga gttagtcttg aagtcatgcg ccggttaagg
    13741 ctaaactgaa aggacaagtt ttggtgactg cgctcctcca agccagttac ctcggttcaa
    13801 agagttggta gctcagagaa ccttcgaaaa accgccctgc aaggcggttt tttcgttttc
    13861 agagcaagag attacgcgca gaccaaaacg atctcaagaa gatcatctta ttaatcagat
    13921 aaaatatttc tagatttcag tgcaatttat ctcttcaaat gtagcacctg aagtcagccc
    13981 catacgatat aagttgttac tagtgcttgg attctcacca ataaaaaacg cccggcggca
    14041 accgagcgtt ctgaacaaat ccagatggag ttctgaggtc attactggat ctatcaacag
    14101 gagtccaagc gagctcgata tcaaattacg ccccgccctg ccactcatcg cagtactgtt
    14161 gtaattcatt aagcattctg ccgacatgga agccatcaca aacggcatga tgaacctgaa
    14221 tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg
    14281 gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg
    14341 gattggctga gacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt
    14401 caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt
    14461 attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt
    14521 gaacactatc ccatatcacc agctcaccgt ctttcattgc catacgaaat tccggatgag
    14581 cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc ttatttttct
    14641 ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag gtacattgag
    14701 caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata tcaacggtgg
    14761 tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat ctcgataact
    14821 caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa cctcttacgt
    14881 gccgatcaac gtctcattt
//
