LOCUS       sequence-275292-        3778 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PgadE - TARGET3 - mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3778)
  AUTHORS   Glasscock CJ, Biggs BW, Lazar JT, Arnold JH, Burdette LA, Valdes A, 
            Kang MK, Tullman-Ercek D, Tyo KEJ, Lucks JB
  TITLE     Dynamic Control of Gene Expression with Riboregulated Switchable 
            Feedback Promoters.
  JOURNAL   ACS Synth Biol. 2021 May 21;10(5):1199-1213. doi: 
            10.1021/acssynbio.1c00015. Epub 2021 Apr 9.
  PUBMED    33834762
REFERENCE   2  (bases 1 to 3778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00015"; journalName: "ACS Synth Biol"; date: 
            "2021-05-21- 21"; volume: "10"; issue: "5"; pages: "1199-1213"
COMMENT     Sequence Label: pJBL6717
FEATURES             Location/Qualifiers
     source          1..3778
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(171..962)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(963..1054)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1102..1840)
                     /direction=LEFT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     CDS             2948..3625
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      3658..3729
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3745..3772
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 ctgaaacctc aggcatttga gaagcacacg gtcacactgc ttccggtagt caataaaccg
       61 gtaaaccagc aatagacata agcggctatt taacgaccct gccctgaacc gacgaccggg
      121 tcatcgtggc cggatcttgc ggcccctcgg cttgaacgaa ttgttagaca ttatttgccg
      181 actaccttgg tgatctcgcc tttcacgtag tggacaaatt cttccaactg atctgcgcgc
      241 gaggccaagc gatcttcttc ttgtccaaga taagcctgtc tagcttcaag tatgacgggc
      301 tgatactggg ccggcaggcg ctccattgcc cagtcggcag cgacatcctt cggcgcgatt
      361 ttgccggtta ctgcgctgta ccaaatgcgg gacaacgtaa gcactacatt tcgctcatcg
      421 ccagcccagt cgggcggcga gttccatagc gttaaggttt catttagcgc ctcaaataga
      481 tcctgttcag gaaccggatc aaagagttcc tccgccgctg gacctaccaa ggcaacgcta
      541 tgttctcttg cttttgtcag caagatagcc agatcaatgt cgatcgtggc tggctcgaag
      601 atacctgcaa gaatgtcatt gcgctgccat tctccaaatt gcagttcgcg cttagctgga
      661 taacgccacg gaatgatgtc gtcgtgcaca acaatggtga cttctacagc gcggagaatc
      721 tcgctctctc caggggaagc cgaagtttcc aaaaggtcgt tgatcaaagc tcgccgcgtt
      781 gtttcatcaa gccttacggt caccgtaacc agcaaatcaa tatcactgtg tggcttcagg
      841 ccgccatcca ctgcggagcc gtacaaatgt acggccagca acgtcggttc gagatggcgc
      901 tcgatgacgc caactacctc tgatagttga gtcgatactt cggcgatcac cgcttccctc
      961 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     1021 tacatatttg aatgtattta gaaaaataaa caaatagcta gctcactcgg tcgctacgct
     1081 ccgggcgtga gactgcggcg ggcgctgcgg acacatacaa agttacccac agattccgtg
     1141 gataagcagg ggactaacat gtgaggcaaa acagcagggc cgcgccggtg gcgtttttcc
     1201 ataggctccg ccctcctgcc agagttcaca taaacagacg cttttccggt gcatctgtgg
     1261 gagccgttag gctcaaccat gaatctgaca gtacgggcga aacccgacag gacttaaaga
     1321 tccccaccgt ttccggcggg tcgctccctc ttgcgctctc ctgttccgac cctgccgttt
     1381 accggatacc tgttccgcct ttctccctta cgggaagtgt ggcgctttct catagctcac
     1441 acactggtat ctcggctcgg tgtaggtcgt tcgctccaag ctgggctgta agcaagaact
     1501 ccccgttcag cccgactgct gcgccttatc cggtaactgt tcacttgagt ccaacccgga
     1561 aaagcacggt aaaacgccac tggcagcagc cattggtaac tgggagttcg cagaggattt
     1621 gtttagctaa acacgcggtt gctcttgaag tgtgcgccaa agtccggcta cactggaagg
     1681 acagatttgg ttgctgtgct ctgcgaaagc cagttaccac ggttaagtca gttggggaac
     1741 tgcttaacct tcgatcaaac cacctcccca ggtggttttt tcgtttacag ggcaaaagat
     1801 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctactg aaccgctcta
     1861 gatttcagtg caatttatct cttcaaatgt agcacctgaa gtcagcccca tacgatataa
     1921 gttgtaattc tcatgttagt catgccccgc gcccacagga aggagctgac tgggttgaag
     1981 gctctcaagg gcatcggtcg agatcccggt gcctaatgag tgagctaact tacattaatt
     2041 gcgttgcgcg aattcacgag atctgcggat gggcagtatt ggcgctgaac gcctgctggc
     2101 gcgtattcgt ggcgttaata ctctctccgc tacgcagtgt tgtagatcaa ttgcgcacta
     2161 tcattgaaat aattacctgc tagtgattat ttcaacctac tgaatttcat ctaatttttt
     2221 tcactctatg gcaaattagc catttcaaac attatcatgg ctgatatttt ccgtagtcag
     2281 gtttaatgtt ttaaaagtgc tgtgggaaag tgaacaaaga gttccgtaag cgttgatgct
     2341 atgggcggtt aaataagtaa tccgggttca tttttttgca actggcgttg attacattgc
     2401 ataaatatcc gtgtctccag acgctatata aaaacctgaa gacatgaatg cgttatttac
     2461 tcaggtaatt tcaatgcgtt aaaagaaagc tggcaatcca attgccagct taagtcgaaa
     2521 caaggagact cgatatttaa atcggattac attttaactt tagtaatatt cttcagagat
     2581 cacaaactgg ttattgataa cttattcttg ggcagtaatc cgcaaacgtt aactttttgt
     2641 ttgctattta caagctgata acaaccagga atcttactta ggatcaatat atggagtgcg
     2701 tgatggataa atctgaagta ttgattagtg ttaatagacg tattagttca cgaagggtaa
     2761 agttcttata ggcgtttact atattgaaca acgattcgga caaggatgta aataatgaaa
     2821 aggatgacat attcgaaacg acctctatcc tatctatctg cctgtcctgt cctgtgtctt
     2881 tgcggggaat gtatacagtt catgtatata ttccccgctt tttttttgga tctaggagga
     2941 aggatctatg gcgagtagcg aagacgttat caaagagttc atgcgtttca aagttcgtat
     3001 ggaaggttcc gttaacggtc acgagttcga aatcgaaggt gaaggtgaag gtcgtccgta
     3061 cgaaggtacc cagaccgcta aactgaaagt taccaaaggt ggtccgctgc cgttcgcttg
     3121 ggacatcctg tccccgcagt tccagtacgg ttccaaagct tacgttaaac acccggctga
     3181 catcccggac tacctgaaac tgtccttccc ggaaggtttc aaatgggaac gtgttatgaa
     3241 cttcgaagac ggtggtgttg ttaccgttac ccaggactcc tccctgcaag acggtgagtt
     3301 catctacaaa gttaaactgc gtggtaccaa cttcccgtcc gacggtccgg ttatgcagaa
     3361 aaaaaccatg ggttgggaag cttccaccga acgtatgtac ccggaagacg gtgctctgaa
     3421 aggtgaaatc aaaatgcgtc tgaaactgaa agacggtggt cactacgacg ctgaagttaa
     3481 aaccacctac atggctaaaa aaccggttca gctgccgggt gcttacaaaa ccgacatcaa
     3541 actggacatc acctcccaca acgaagacta caccatcgtt gaacagtacg aacgtgctga
     3601 aggtcgtcac tccaccggtg cttaaggatc caaactcgag taaggatctc caggcatcaa
     3661 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
     3721 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata
//
