LOCUS       sequence-275306-       19513 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid with 2 different NOP1-gRNA. Targets 500 bp apart in the 
            genome. To tranform Mp as CRISPR control. Contains Cas9 and HygR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19513)
  AUTHORS   Sauret-Gueto S, Frangedakis E, Silvestri L, Rebmann M, Tomaselli M, 
            Markel K, Delmans M, West A, Patron NJ, Haseloff J
  TITLE     Systematic tools for reprogramming plant gene expression in a simple
            model, Marchantia polymorpha.
  JOURNAL   ACS Synth Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511.
  PUBMED    32163700
REFERENCE   2  (bases 1 to 19513)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511."
COMMENT     Sequence Label: L2_2xNOP1gRNA-Cas9-CsA
FEATURES             Location/Qualifiers
     source          1..19513
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    433..457
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        976..1321
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1264..1284
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             1352..2377
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    2412..2586
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_RNA        4631..4706
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    complement(4718..4742)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_RNA        6788..6863
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    complement(6875..6899)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             8649..12752
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             8649..12752
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             12765..12785
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      12809..13061
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     polyA_signal    13108..13282
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    13308..13332
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             14633..15262
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             15691..16764
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      16830..17024
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(17260..17282)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    17368..17508
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     17423..17442
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(17523..17540)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(17694..18281)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(17774..17793)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(18369..19160)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 aacagaactt attatttcct tcctcttttc tacagtattt aaagataccc caagaagcta
       61 attataacaa gacgaactcc aattcactgt tccttgcatt ctaaaacctt aaataccaga
      121 aaacagcttt ttcaaagttg ttttcaaagt tggcgtataa catagtatcg acggagccga
      181 ttttgaaacc gcggtgatca caggcagcaa cgctctgtca tcgttacaat caacatgcta
      241 ccctccgcga gatcatccgt gtttcaaacc cggcagctta gttgccgttc ttccgaatag
      301 catcggtaac atgagcaaag tctgccgcct tacaacggct ctcccgctga cgccgtcccg
      361 gactgatggg ctgcctgtat cgagtggtga ttttgtgccg agctgccggt cggggagctg
      421 ttggctggct ggtggcagga tatattgtgg tgtaaacata acggatccgg tctcaggaga
      481 atgaggagag attagccttt tcaatttcag aaagaatgct aacccacaga tggttagaga
      541 ggcttacgca gcaagtctca tcaagacgat ctacccgagc aataatctac aggaaatcaa
      601 ataccttccc aagaaggtta aagatgcagt caaaagattc aggactaact gcatcaagaa
      661 cacagagaaa gatatatttc tcaagatcag aagtactatt ccagtatgga cgattcaagg
      721 cttgcttcac aaaccaaggc aagtaataga gattggagtc tctaaaaagg tagttcccac
      781 tgaatcaaag gccatggagt caaagattca aatagaggac ctaacagaac tcgccgtaaa
      841 gactggcgaa cagttcatac agagtctctt acgactcaat gacaagaaga aaatcttcgt
      901 caacatggtg gagcacgaca cacttgtcta ctccaaaaat atcaaagata cagtctcagt
      961 agaccaaagg gcaattgaga cttttcaaca aagggtaata tccggaaacc tcctcggatt
     1021 ccattgccca gctatctgtc actttattgt gaagatagtg gaaaaggaag gtggctccta
     1081 caaatgccat cattgcgata aaggaaaggc catcgttgaa gatgcctctg ccgacagtgg
     1141 tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaaaacg ttccaaccac
     1201 gtctacaaag caagtggatt gatgtgatat ctccactgac gtaagggatg acgcacaatc
     1261 ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagagaac
     1321 acgggggact ctagaggatc caacatcacc aatgaaaaag cctgaactca ccgcgacgtc
     1381 tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc agctctcgga
     1441 gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt
     1501 aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact ttgcatcggc
     1561 cgcgctcccg attccggaag tgcttgacat tggggagttt agcgagagcc tgacctattg
     1621 catctcccgc cgttcacagg gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc
     1681 tgttctacaa ccggtcgcgg aggctatgga tgcgatcgct gcggccgatc ttagccagac
     1741 gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa tacactacat ggcgtgattt
     1801 catatgcgcg attgctgatc cccatgtgta tcactggcaa actgtgatgg acgacaccgt
     1861 cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg actgccccga
     1921 agtccggcac ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg acaatggccg
     1981 cataacagcg gtcattgact ggagcgaggc gatgttcggg gattcccaat acgaggtcgc
     2041 caacatcttc ttctggaggc cgtggttggc ttgtatggag cagcagacgc gctacttcga
     2101 gcggaggcat ccggagcttg caggatcgcc acgactccgg gcgtatatgc tccgcattgg
     2161 tcttgaccaa ctctatcaga gcttggttga cggcaatttc gatgatgcag cttgggcgca
     2221 gggtcgatgc gacgcaatcg tccgatccgg agccgggact gtcgggcgta cacaaatcgc
     2281 ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa
     2341 ccgacgcccc agcactcgtc cgagggcaaa gaaataggct tctctagcta gagtcgatcg
     2401 acaagctcga gtttctccat aataatgtgt gagtagttcc cagataaggg aattagggtt
     2461 cctatagggt ttcgctcatg tgttgagcat ataagaaacc cttagtatgt atttgtattt
     2521 gtaaaatact tctatcaata aaatttctaa ttcctaaaac caaaatccag tactaaaatc
     2581 cagatcgcta gcaaggagta ttcattcaaa agagattttt aaagatcaat ttttctttct
     2641 cataagtcta atacgtcatg ataataactt taaaaaaaat tgaactcgtt aaaaaatttg
     2701 atcaaatttt aatttattat tagatcatat attacatatc agtttcataa tgttatacta
     2761 agatctttca tatatatttt aacccacgag acaaaatatg tttatatctt tatttattta
     2821 aatatttttt agctcaaaac atcgcaaact cagctatcat aatgcttttt aatgaaaaaa
     2881 acaaattttc ctatcatact ctcattgttt ataatttttt agaaaaaaaa cttgaagata
     2941 aaataacaac aataatagtt tcaaaagcac atttttgttg taaatttcaa gaaaaataca
     3001 atcatattac tgattgacta caaaaatttg taaattaatc caggctacat gtttaagatg
     3061 tgtcaaaact gcatcatcta catctcattt taatttttct aaactacata catagtatca
     3121 ataacaattg aacaatgcta gttaatgtgt tatactcatt agcttgtaat atatcttcaa
     3181 cttgatgaat cggattgatc ataaaatttc acaaacttat gtgctgatta ataaaaaata
     3241 taatcagatt tttcatgaat aattaagata aataacacat gcaagtctca attagaattt
     3301 aacaatctga ctataaaaag tcggtttatc ttgattttca caaaaattta acatagtttc
     3361 aaggtgaatg ggcttttatt ttaatccaat caactatttg acacatacat cttgcaaatt
     3421 tatttatttt ttatggaaaa aaacatgatt tatgtttttc tttgagaaag tatattgtgg
     3481 attgtcaaaa aaaacctatg gaagatttat atgtaagagt taaaaattta ttttcaaata
     3541 tgtcaacaat ccacaatatc gaagaaaatt ttcaaaaaat catcacatat gatgtaaata
     3601 taatatgaat atgtgcaatc ttatttcatt ttactctgaa aacttaattt ctttttatag
     3661 agcattctgt acttactggt gtcaaatata cagataaaat gcagtccata acgtatcact
     3721 ctataagcaa aatcataaac ttcgtgatct ctcacagttc aaacgagaac tatagaaacc
     3781 cttcaactag caaaaaactc acaactttct attccaatat ctttaattgt ctgacgattt
     3841 acaaatagaa acgaaaacca gacacacaca cacacgcaaa ctacaaagta aaaacacaac
     3901 cacctgacat tagacagtga gattattaaa catcctaaac taaaaaacaa acaaattaat
     3961 tctctatgta caagtcccca tcgtgatcaa tttttgcatc ccaatatagt ggcggttagt
     4021 ataagaggac cttcgtctat tcactctcca tgtttgtcaa actatagtac agtacatata
     4081 tgtatgggaa caaattccga acactctagt acactcctca acgcaagttc tatctcatat
     4141 ctctgcaaga gcttattcca agaaactttc ttgtgcttct atgcatgccc aggtacctta
     4201 agtcgcgcgc atagtcactt cacagccttt tcagttctcc ctttgatggc tcagagtgac
     4261 gcaactgcac aaaaataaga aaaaagtcga agcgtcacga gagatgtatc cggctagttt
     4321 ccttggagtt cggattgctc tctttctctg tctccccgaa ccatatggaa tttatgaact
     4381 gctgttcact agtcagtcac ttgtttcaga acttttgagt ggtcgattta tccaccttct
     4441 tgtttcttcc tgggtccaga atctgacttc ttgagacaac aaacagtgca cagtggatcg
     4501 aagaatgacc acagttgaga cacgtggcgc tggcctcatt tattgcacca tacgtttagt
     4561 gaagaactga ataatggtgg atataaagca gagttgcacc cagcctctcg attaagagtg
     4621 gaagttgctt gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac
     4681 ttgaaaaagt ggcaccgagt cggtgctttt tttctagacc cagctttctt gtacaaagtt
     4741 ggccgctata caggagtatt cattcaaaag agatttttaa agatcaattt ttctttctca
     4801 taagtctaat acgtcatgat aataacttta aaaaaaattg aactcgttaa aaaatttgat
     4861 caaattttaa tttattatta gatcatatat tacatatcag tttcataatg ttatactaag
     4921 atctttcata tatattttaa cccacgagac aaaatatgtt tatatcttta tttatttaaa
     4981 tattttttag ctcaaaacat cgcaaactca gctatcataa tgctttttaa tgaaaaaaac
     5041 aaattttcct atcatactct cattgtttat aattttttag aaaaaaaact tgaagataaa
     5101 ataacaacaa taatagtttc aaaagcacat ttttgttgta aatttcaaga aaaatacaat
     5161 catattactg attgactaca aaaatttgta aattaatcca ggctacatgt ttaagatgtg
     5221 tcaaaactgc atcatctaca tctcatttta atttttctaa actacataca tagtatcaat
     5281 aacaattgaa caatgctagt taatgtgtta tactcattag cttgtaatat atcttcaact
     5341 tgatgaatcg gattgatcat aaaatttcac aaacttatgt gctgattaat aaaaaatata
     5401 atcagatttt tcatgaataa ttaagataaa taacacatgc aagtctcaat tagaatttaa
     5461 caatctgact ataaaaagtc ggtttatctt gattttcaca aaaatttaac atagtttcaa
     5521 ggtgaatggg cttttatttt aatccaatca actatttgac acatacatct tgcaaattta
     5581 tttatttttt atggaaaaaa acatgattta tgtttttctt tgagaaagta tattgtggat
     5641 tgtcaaaaaa aacctatgga agatttatat gtaagagtta aaaatttatt ttcaaatatg
     5701 tcaacaatcc acaatatcga agaaaatttt caaaaaatca tcacatatga tgtaaatata
     5761 atatgaatat gtgcaatctt atttcatttt actctgaaaa cttaatttct ttttatagag
     5821 cattctgtac ttactggtgt caaatataca gataaaatgc agtccataac gtatcactct
     5881 ataagcaaaa tcataaactt cgtgatctct cacagttcaa acgagaacta tagaaaccct
     5941 tcaactagca aaaaactcac aactttctat tccaatatct ttaattgtct gacgatttac
     6001 aaatagaaac gaaaaccaga cacacacaca cacgcaaact acaaagtaaa aacacaacca
     6061 cctgacatta gacagtgaga ttattaaaca tcctaaacta aaaaacaaac aaattaattc
     6121 tctatgtaca agtccccatc gtgatcaatt tttgcatccc aatatagtgg cggttagtat
     6181 aagaggacct tcgtctattc actctccatg tttgtcaaac tatagtacag tacatatatg
     6241 tatgggaaca aattccgaac actctagtac actcctcaac gcaagttcta tctcatatct
     6301 ctgcaagagc ttattccaag aaactttctt gtgcttctat gcatgcccag gtaccttaag
     6361 tcgcgcgcat agtcacttca cagccttttc agttctccct ttgatggctc agagtgacgc
     6421 aactgcacaa aaataagaaa aaagtcgaag cgtcacgaga gatgtatccg gctagtttcc
     6481 ttggagttcg gattgctctc tttctctgtc tccccgaacc atatggaatt tatgaactgc
     6541 tgttcactag tcagtcactt gtttcagaac ttttgagtgg tcgatttatc caccttcttg
     6601 tttcttcctg ggtccagaat ctgacttctt gagacaacaa acagtgcaca gtggatcgaa
     6661 gaatgaccac agttgagaca cgtggcgctg gcctcattta ttgcaccata cgtttagtga
     6721 agaactgaat aatggtggat ataaagcaga gttgcaccca gcctctcgat agtctttgtg
     6781 agagaatgtt ttagagctag aaatagcaag ttaaaataag gctagtccgt tatcaacttg
     6841 aaaaagtggc accgagtcgg tgcttttttt ctagacccag ctttcttgta caaagttggc
     6901 cgctacagag gagcaaatga gtcacacaca ttgttgagag acatatcaaa ataactcaat
     6961 tgtaaggtaa gttgatagat ttcccagtta aatgcattat tttaacaata tttgtatctc
     7021 aaattttcaa aatcatatca aatttgcaga tcttgatgat gattcacaac tctgtcttcg
     7081 atttttgatt gttaacgggt atcgatattt gggtaactta aaaagaattt gctaaagatc
     7141 ttaaaaaaca gattaaaaaa acctttcaaa atagctaatc ctttaaatcc gtttcacaat
     7201 ataatgctca ttgatgataa tcgaaaagat tccattttaa agtgcatata aattgcttaa
     7261 ttatctcttt ctgtctcgta atattttgtt ctctcctatt ggatcggacc cgcatcttct
     7321 cgatcagttc actatactaa tatttggtca acttcaagag agattagaac ttcgttctcc
     7381 aaagtgtaaa ggccgacaag cagaaaagga aacttcaatc agaagccttc taggttttcc
     7441 cgcgtcccaa aaagcgaagg gaggacgggg aggacggtga ggccagaaga agaaagcaac
     7501 agtaaaatct catgtgattg cgtgcatgtc atggatctat gggatgctag tccaccgttt
     7561 ggaagtatgt gcagaaggag aaaggcgcgg gaggccaaaa gcgacttcgg ccacctctct
     7621 actttctaga aagtttgctc gcagcttgta ataagcgcgg aaaaggtact cgaaaaaccg
     7681 gagcaatcgg tgtggtatca gcagtcgggt tcgctaaacc tacgtcttct tattttggaa
     7741 tgttaaattg aaagggcgtg ccggccctaa gcacggacga tgtgacagac ggaggagtgt
     7801 gagcaattcg gatgacgatg ctggccggca gtcacgacaa agagtccgaa ggggcatgga
     7861 cagggcaata tataaatgga aaagaggctt tgaatattcc taacccttcg tcgtcggcct
     7921 cccaacttgc cctcgcatgc tgaattggcc gcctatctgc gaatggccgg ccgccgaccc
     7981 taagtcgcaa taacttgaac ggagccgatg caaagtcctt atcgaacgct cgttattata
     8041 aacggggcag acctaggtat ttaattatat caaaaccctc cgcaatttcg ctaatcattc
     8101 cctaatttct tctactttgg cgtctagtgc tgtggtcgcg cttgctctca aagctcgctg
     8161 ggaggcagtg gagcgtctgg cttaaaatcg atcgccgcaa ccctagagtt cctttcactt
     8221 ctttctctct tgcgtggaga gcatcgacat cgaggagctg ctacttgtct tctggtatgc
     8281 gccttgtgcc ctcgtctctg tacgtcgtta cgattgctcg atattgtgat tgtctagacc
     8341 tgaaacccga ctggagcttt tacgtggtgt cgattggggc ctcgagcgag tgatttttta
     8401 ggttcgtttt agccaagtac atcccacttt ggtcagtcct gttatctcgg taaggtgttc
     8461 agttcggtca ctggtgcgga ggtgcctcgt aagtttcctg catgatagat ttgtctatcc
     8521 ttgacttgaa gaggagtcga aggtcagcga ggttggctgt gtgctatgga cgtcggtgct
     8581 ccttggttcc gtgcttctat gggtgttgcc tcaacagtat tgatgtgcct gcagaaaggt
     8641 tgtcaccaat ggataagaag tactctatcg gactcgatat cggaactaac tctgtgggat
     8701 gggctgtgat caccgatgag tacaaggtgc catctaagaa gttcaaggtt ctcggaaaca
     8761 ccgataggca ctctatcaag aaaaacctta tcggtgctct cctcttcgat tctggtgaaa
     8821 ctgctgaggc taccagactc aagagaaccg ctagaagaag gtacaccaga agaaagaaca
     8881 ggatctgcta cctccaagag atcttctcta acgagatggc taaagtggat gattcattct
     8941 tccacaggct cgaagagtca ttcctcgtgg aagaagataa gaagcacgag aggcacccta
     9001 tcttcggaaa catcgttgat gaggtggcat accacgagaa gtaccctact atctaccacc
     9061 tcagaaagaa gctcgttgat tctactgata aggctgatct caggctcatc tacctcgctc
     9121 tcgctcacat gatcaagttc agaggacact tcctcatcga gggtgatctc aaccctgata
     9181 actctgatgt ggataagttg ttcatccagc tcgtgcagac ctacaaccag cttttcgaag
     9241 agaaccctat caacgcttca ggtgtggatg ctaaggctat cctctctgct aggctctcta
     9301 agtcaagaag gcttgagaac ctcattgctc agctccctgg tgagaagaag aacggacttt
     9361 tcggaaactt gatcgctctc tctctcggac tcacccctaa cttcaagtct aacttcgatc
     9421 tcgctgagga tgcaaagctc cagctctcaa aggataccta cgatgatgat ctcgataacc
     9481 tcctcgctca gatcggagat cagtacgctg atttgttcct cgctgctaag aacctctctg
     9541 atgctatcct cctcagtgat atcctcagag tgaacaccga gatcaccaag gctccactct
     9601 cagcttctat gatcaagaga tacgatgagc accaccagga tctcacactt ctcaaggctc
     9661 ttgttagaca gcagctccca gagaagtaca aagagatttt cttcgatcag tctaagaacg
     9721 gatacgctgg ttacatcgat ggtggtgcat ctcaagaaga gttctacaag ttcatcaagc
     9781 ctatcctcga gaagatggat ggaaccgagg aactcctcgt gaagctcaat agagaggatc
     9841 ttctcagaaa gcagaggacc ttcgataacg gatctatccc tcatcagatc cacctcggag
     9901 agttgcacgc tatccttaga aggcaagagg atttctaccc attcctcaag gataacaggg
     9961 aaaagattga gaagattctc accttcagaa tcccttacta cgtgggacct ctcgctagag
    10021 gaaactcaag attcgcttgg atgaccagaa agtctgagga aaccatcacc ccttggaact
    10081 tcgaagaggt ggtggataag ggtgctagtg ctcagtcttt catcgagagg atgaccaact
    10141 tcgataagaa ccttccaaac gagaaggtgc tccctaagca ctctttgctc tacgagtact
    10201 tcaccgtgta caacgagttg accaaggtta agtacgtgac cgagggaatg aggaagcctg
    10261 cttttttgtc aggtgagcaa aagaaggcta tcgttgatct cttgttcaag accaacagaa
    10321 aggtgaccgt gaagcagctc aaagaggatt acttcaagaa aatcgagtgc ttcgattcag
    10381 ttgagatttc tggtgttgag gataggttca acgcatctct cggaacctac cacgatctcc
    10441 tcaagatcat taaggataag gatttcttgg ataacgagga aaacgaggat atcttggagg
    10501 atatcgttct taccctcacc ctctttgaag atagagagat gattgaagaa aggctcaaga
    10561 cctacgctca tctcttcgat gataaggtga tgaagcagtt gaagagaaga agatacactg
    10621 gttggggaag gctctcaaga aagctcatta acggaatcag ggataagcag tctggaaaga
    10681 caatccttga tttcctcaag tctgatggat tcgctaacag aaacttcatg cagctcatcc
    10741 acgatgattc tctcaccttt aaagaggata tccagaaggc tcaggtttca ggacagggtg
    10801 atagtctcca tgagcatatc gctaacctcg ctggatctcc tgcaatcaag aagggaatcc
    10861 tccagactgt gaaggttgtg gatgagttgg tgaaggtgat gggaaggcat aagcctgaga
    10921 acatcgtgat cgaaatggct agagagaacc agaccactca gaagggacag aagaactcta
    10981 gggaaaggat gaagaggatc gaggaaggta tcaaagagct tggatctcag atcctcaaag
    11041 agcaccctgt tgagaacact cagctccaga atgagaagct ctacctctac tacctccaga
    11101 acggaaggga tatgtatgtg gatcaagagt tggatatcaa caggctctct gattacgatg
    11161 ttgatcatat cgtgccacag tcattcttga aggatgattc tatcgataac aaggtgctca
    11221 ccaggtctga taagaacagg ggtaagagtg ataacgtgcc aagtgaagag gttgtgaaga
    11281 aaatgaagaa ctattggagg cagctcctca acgctaagct catcactcag agaaagttcg
    11341 ataacttgac taaggctgag aggggaggac tctctgaatt ggataaggca ggattcatca
    11401 agaggcagct tgtggaaacc aggcagatca ctaagcacgt tgcacagatc ctcgattcta
    11461 ggatgaacac caagtacgat gagaacgata agttgatcag ggaagtgaag gttatcaccc
    11521 tcaagtcaaa gctcgtgtct gatttcagaa aggatttcca attctacaag gtgagggaaa
    11581 tcaacaacta ccaccacgct cacgatgctt accttaacgc tgttgttgga accgctctca
    11641 tcaagaagta tcctaagctc gagtcagagt tcgtgtacgg tgattacaag gtgtacgatg
    11701 tgaggaagat gatcgctaag tctgagcaag agatcggaaa ggctaccgct aagtatttct
    11761 tctactctaa catcatgaat ttcttcaaga ccgagattac cctcgctaac ggtgagatca
    11821 gaaagaggcc actcatcgag acaaacggtg aaacaggtga gatcgtgtgg gataagggaa
    11881 gggatttcgc taccgttaga aaggtgctct ctatgccaca ggtgaacatc gttaagaaaa
    11941 ccgaggtgca gaccggtgga ttctctaaag agtctatcct ccctaagagg aactctgata
    12001 agctcattgc taggaagaag gattgggacc ctaagaaata cggtggtttc gattctccta
    12061 ccgtggctta ctctgttctc gttgtggcta aggttgagaa gggaaagagt aagaagctca
    12121 agtctgttaa ggaacttctc ggaatcacta tcatggaaag gtcatctttc gagaagaacc
    12181 caatcgattt cctcgaggct aagggataca aagaggttaa gaaggatctc atcatcaagc
    12241 tcccaaagta ctcactcttc gaactcgaga acggtagaaa gaggatgctc gcttctgctg
    12301 gtgagcttca aaagggaaac gagcttgctc tcccatctaa gtacgttaac tttctttacc
    12361 tcgcttctca ctacgagaag ttgaagggat ctccagaaga taacgagcag aagcaacttt
    12421 tcgttgagca gcacaagcac tacttggatg agatcatcga gcagatctct gagttctcta
    12481 aaagggtgat cctcgctgat gcaaacctcg ataaggtgtt gtctgcttac aacaagcaca
    12541 gagataagcc tatcagggaa caggcagaga acatcatcca tctcttcacc cttaccaacc
    12601 tcggtgctcc tgctgctttc aagtacttcg atacaaccat cgataggaag agatacacct
    12661 ctaccaaaga agtgctcgat gctaccctca tccatcagtc tatcactgga ctctacgaga
    12721 ctaggatcga tctctcacag ctcggtggtg attcaagggc tgatcctaag aagaagagga
    12781 aggtttgagc ttgagctcga atttccccga tcgttcaaac atttggcaat aaagtttctt
    12841 aagattgaat cctgttgccg gtcttgcgat gattatcata taatttctgt tgaattacgt
    12901 taagcatgta ataattaaca tgtaatgcat gacgttattt atgagatggg tttttatgat
    12961 tagagtcccg caattataca tttaatacgc gatagaaaac aaaatatagc gcgcaaacta
    13021 ggataaatta tcgcgcgcgg tgtcatctat gttactagat cgatccgtat cgatagcctc
    13081 tagctagagt cgatcgacaa gctcgagttt ctccataata atgtgtgagt agttcccaga
    13141 taagggaatt agggttccta tagggtttcg ctcatgtgtt gagcatataa gaaaccctta
    13201 gtatgtattt gtatttgtaa aatacttcta tcaataaaat ttctaattcc taaaatcaaa
    13261 atccagtact aaaatccaga tcgctaggta tacttgagac cagatcctga caggatatat
    13321 tggcgggtaa acctaagaga aaagagcgtt tattagaata atcggatatt taaaagggcg
    13381 tgaaaaggtt tatccgttcg tccatttgta tgtgcatgcc aaccacaggg ttcccctcgg
    13441 gatcaaagta ctttgatcca acccctccgc tgctatagtg cagtcggctt ctgacgttca
    13501 gtgcagccgt catctgaaaa cgacatgtcg cacaagtcct aagttacgcg acaggctgcc
    13561 gccctgccct tttcctggcg ttttcttgtc gcgtgtttta gtcgcataaa gtagaatact
    13621 tgcgactaga accggagaca ttacgccatg aacaagagcg ccgccgctgg cctgctgggc
    13681 tatgcccgcg tcagcaccga cgaccaggac ttgaccaacc aacgggccga actgcacgcg
    13741 gccggctgca ccaagctgtt ttccgagaag atcaccggca ccaggcgcga ccgcccggag
    13801 ctggccagga tgcttgacca cctacgccct ggcgacgttg tgacagtgac caggctagac
    13861 cgcctggccc gcagcacccg cgacctactg gacattgccg agcgcatcca ggaggccggc
    13921 gcgggcctgc gtagcctggc agagccgtgg gccgacacca ccacgccggc cggccgcatg
    13981 gtgttgaccg tgttcgccgg cattgccgag ttcgagcgtt ccctaatcat cgaccgcacc
    14041 cggagcgggc gcgaggccgc caaggcccga ggcgtgaagt ttggcccccg ccctaccctc
    14101 accccggcac agatcgcgca cgcccgcgag ctgatcgacc aggaaggccg caccgtgaaa
    14161 gaggcggctg cactgcttgg cgtgcatcgc tcgaccctgt accgcgcact tgagcgcagc
    14221 gaggaagtga cgcccaccga ggccaggcgg cgcggtgcct tccgtgagga cgcattgacc
    14281 gaggccgacg ccctggcggc cgccgagaat gaacgccaag aggaacaagc atgaaaccgc
    14341 accaggacgg ccaggacgaa ccgtttttca ttaccgaaga gatcgaggcg gagatgatcg
    14401 cggccgggta cgtgttcgag ccgcccgcgc acctctcaac cgtgcggctg catgaaatcc
    14461 tggccggttt gtctgatgcc aagctggcgg cctggccggc cagcttggcc gctgaagaaa
    14521 ccgagcgccg ccgtctaaaa aggtgatgtg tatttgagta aaacagcttg cgtcatgcgg
    14581 tcgctgcgta tatgatgcga tgagtaaata aacaaatacg caaggggaac gcatgaaggt
    14641 tatcgctgta cttaaccaga aaggcgggtc aggcaagacg accatcgcaa cccatctagc
    14701 ccgcgccctg caactcgccg gggccgatgt tctgttagtc gattccgatc cccagggcag
    14761 tgcccgcgat tgggcggccg tgcgggaaga tcaaccgcta accgttgtcg gcatcgaccg
    14821 cccgacgatt gaccgcgacg tgaaggccat cggccggcgc gacttcgtag tgatcgacgg
    14881 agcgccccag gcggcggact tggctgtgtc cgcgatcaag gcagccgact tcgtgctgat
    14941 tccggtgcag ccaagccctt acgacatatg ggccaccgcc gacctggtgg agctggttaa
    15001 gcagcgcatt gaggtcacgg atggaaggct acaagcggcc tttgtcgtgt cgcgggcgat
    15061 caaaggcacg cgcatcggcg gtgaggttgc cgaggcgctg gccgggtacg agctgcccat
    15121 tcttgagtcc cgtatcacgc agcgcgtgag ctacccaggc actgccgccg ccggcacaac
    15181 cgttcttgaa tcagaacccg agggcgacgc tgcccgcgag gtccaggcgc tggccgctga
    15241 aattaaatca aaactcattt gagttaatga ggtaaagaga aaatgagcaa aagcacaaac
    15301 acgctaagtg ccggccgtcc gagcgcacgc agcagcaagg ctgcaacgtt ggccagcctg
    15361 gcagacacgc cagccatgaa gcgggtcaac tttcagttgc cggcggagga tcacaccaag
    15421 ctgaagatgt acgcggtacg ccaaggcaag accattaccg agctgctatc tgaatacatc
    15481 gcgcagctac cagagtaaat gagcaaatga ataaatgagt agatgaattt tagcggctaa
    15541 aggaggcggc atggaaaatc aagaacaacc aggcaccgac gccgtggaat gccccatgtg
    15601 tggaggaacg ggcggttggc caggcgtaag cggctgggtt gcctgccggc cctgcaatgg
    15661 cactggaacc cccaagcccg aggaatcggc gtgagcggtc gcaaaccatc cggcccggta
    15721 caaatcggcg cggcgctggg tgatgacctg gtggagaagt tgaaggccgc gcaggccgcc
    15781 cagcggcaac gcatcgaggc agaagcacgc cccggtgaat cgtggcaagc ggccgctgat
    15841 cgaatccgca aagaatcccg gcaaccgccg gcagccggtg cgccgtcgat taggaagccg
    15901 cccaagggcg acgagcaacc agattttttc gttccgatgc tctatgacgt gggcacccgc
    15961 gatagtcgca gcatcatgga cgtggccgtt ttccgtctgt cgaagcgtga ccgacgagct
    16021 ggcgaggtga tccgctacga gcttccagac gggcacgtag aggtttccgc agggccggcc
    16081 ggcatggcca gtgtgtggga ttacgacctg gtactgatgg cggtttccca tctaaccgaa
    16141 tccatgaacc gataccggga agggaaggga gacaagcccg gccgcgtgtt ccgtccacac
    16201 gttgcggacg tactcaagtt ctgccggcga gccgatggcg gaaagcagaa agacgacctg
    16261 gtagaaacct gcattcggtt aaacaccacg cacgttgcca tgcagcgtac gaagaaggcc
    16321 aagaacggcc gcctggtgac ggtatccgag ggtgaagcct tgattagccg ctacaagatc
    16381 gtaaagagcg aaaccgggcg gccggagtac atcgagatcg agctagctga ttggatgtac
    16441 cgcgagatca cagaaggcaa gaacccggac gtgctgacgg ttcaccccga ttactttttg
    16501 atcgatcccg gcatcggccg ttttctctac cgcctggcac gccgcgccgc aggcaaggca
    16561 gaagccagat ggttgttcaa gacgatctac gaacgcagtg gcagcgccgg agagttcaag
    16621 aagttctgtt tcaccgtgcg caagctgatc gggtcaaatg acctgccgga gtacgatttg
    16681 aaggaggagg cggggcaggc tggcccgatc ctagtcatgc gctaccgcaa cctgatcgag
    16741 ggcgaagcat ccgccggttc ctaatgtacg gagcagatgc tagggcaaat tgccctagca
    16801 ggggaaaaag gtcgaaaagg actctttcct gtggatagca cgtacattgg gaacccaaag
    16861 ccgtacattg ggaaccggaa cccgtacatt gggaacccaa agccgtacat tgggaaccgg
    16921 tcacacatgt aagtgactga tataaaagag aaaaaaggcg atttttccgc ctaaaactct
    16981 ttaaaactta ttaaaactct taaaacccgc ctggcctgtg cataactgtc tggccagcgc
    17041 acagccgaag tgctgcaaaa agcgcctacc cttcggtcgc tgcgctccct acgccccgcc
    17101 gcttcgcgtc ggcctatcgc ggccgctggc cgctcaaaaa tggctggcct acggccaggc
    17161 aatctaccag ggcgcggaca agccgcgccg tcgccactcg accgccggcg cccacatcaa
    17221 ggcaccctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
    17281 ggtgacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
    17341 gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg
    17401 agtgtatact ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg
    17461 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcatccgct
    17521 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
    17581 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
    17641 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
    17701 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
    17761 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
    17821 gttccgaccc tgccgcttac cgatacctgt ccgcctttct cccttcggga agcgtggcgc
    17881 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
    17941 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
    18001 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
    18061 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
    18121 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
    18181 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
    18241 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
    18301 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgcatt
    18361 ctaggtgatt atttgccgac taccttggtg atctcgcctt tcacgtagtg gacaaattct
    18421 tccaactgat ctgcgcgcga ggccaagcga tcttcttctt gtccaagata agcctgtcta
    18481 gcttcaagta tgacgggctg atactgggcc ggcaggcgct ccattgccca gtcggcagcg
    18541 acatccttcg gcgcgatttt gccggttact gcgctgtacc aaatgcggga caacgtaagc
    18601 actacatttc gctcatcacc agcccagtcg ggcggcgagt tccatagcgt taaggtttca
    18661 tttagcgcct caaatagatc ctgttcagga accggatcaa agagttcctc cgccgctgga
    18721 cctaccaagg caacgctatg ttctcttgct tttgtcagca agatagccag atcaatgtcg
    18781 atcgtggctg gctcgaagat acctgcaaga atgtcattgc gctgccattc tccaaattgc
    18841 agttcgcgct tagctggata acgccacgga atgatgtcgt cgtgcacaac aatggtgact
    18901 tctacagcgc ggagaatctc gctctctcca ggggaagccg aagtttccaa aaggtcgttg
    18961 atcaaagctc gccgcgttgt ttcatcaagc cttacggtca ccgtaaccag caaatcaata
    19021 tcactgtgtg gcttcaggcc gccatccact gcggagccgt acaaatgtac ggccagcaac
    19081 gtcggttcga gatggcgctc gatgacgcca actacctctg atagttgagt cgatacttcg
    19141 gcgatcaccg cttccctcat aatgtttaac tttgttttag ggcgactgcc ctgctgcgta
    19201 acatcgttgc tgctccataa catcaaacat cgacccacgg cgtaacgcgc ttgctgcttg
    19261 gatgcccgag gcatagactg taccccaaaa aaacagtcat aacaagccat gaaaaccgcc
    19321 actgcgccgt taccaccgct gcgttcggtc aaggttctgg accagttgcg tgagcgcata
    19381 cgctacttgc attacagctt acgaaccgaa caggcttatg tccactgggt tcgtgccttc
    19441 atccgtttcc acggtgtgcg tcacccggca accttgggta gcagcgaagt cgaggcattt
    19501 ctgtcctggc tgg
//
