LOCUS       sequence-27533-e         686 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 686)
  TITLE     Glick lab plasmids
REFERENCE   2  (bases 1 to 686)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: YIPlac204TKC-DsRed-Express2-HDEL
FEATURES             Location/Qualifiers
     source          1..686
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      104..113
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(269..677)
                     /gene="S. cerevisiae TPI1"
                     /label=TPI1 promoter
                     /note="strong constitutive promoter for yeast triose 
                     phosphate isomerase"
ORIGIN
        1 ttgccctcgc cctcgccctc gatctcgaac tcgtggccgt tcacggagcc ctccatgtgc
       61 accttgaagc gcatgaaggg cttgatgacg ttctcagtgc tatccatggt ggcgactagt
      121 ggatccccat catcggcacc tctaactaaa acattggagg agtggaaaga attctgtaaa
      181 ggtaatatta ccacgaaaag ggcgtacagg accacggaga gtggtaccag cagcttgcca
      241 gcgcttagtc tgttgaaaaa catttttagt ttatgtatgt gttttttgta gttatagatt
      301 taagcaagaa aagaatacaa acaaaaaatt gaaaaagatt gatttagaat taaaaagaaa
      361 aatatttacg taagaaggga aaatagtaaa tgttgcaagt tcactaaact cctaaattat
      421 gctgcccttt atattccctg ttacagcagc cgagccaaag gtatataggc tcctttgcat
      481 tagcatgcgt aacaaaccac ctgtcagttt caaccgaggt ggtatccgag agaattgtgt
      541 gattgcttta attaatttcg gagaatctca catgccactg aagattaaaa actggatgcc
      601 agaaangggg tgtccaggtg taacatcaat agaggaagct gaaagtctta gaacgggtat
      661 cttcaacaac ctgatgggtc ctagat
//
