LOCUS       sequence-275353-        9933 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivector encoding Flag-tagged human SNRNP40.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9933)
  AUTHORS   Zhang D, Yue T, Choi JH, Nair-Gill E, Zhong X, Wang KW, Zhan X, Li 
            X, Choi M, Tang M, Quan J, Hildebrand S, Moresco EMY, Beutler B
  TITLE     Syndromic immune disorder caused by a viable hypomorphic allele of 
            spliceosome component Snrnp40.
  JOURNAL   Nat Immunol. 2019 Oct;20(10):1322-1334. doi: 
            10.1038/s41590-019-0464-4. Epub 2019 Aug 19.
  PUBMED    31427773
REFERENCE   2  (bases 1 to 9933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41590-019-0464-4"; journalName: "Nat Immunol"; date: 
            "2019-10"; volume: "20"; issue: "10"; pages: "1322-1334"
COMMENT     Sequence Label: pLV-puro-N-Flag-h-SNRNP40
FEATURES             Location/Qualifiers
     source          1..9933
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        9..235
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             236..416
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    463..588
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1081..1314
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1499..1543
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1810..1926
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2130..3312
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2361..3303
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3260..3280
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3342..3351
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3351..3374
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    4564..5137
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4587..5139
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4731..4748)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4958..4977
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5171..5770
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5171..5190)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5667..5687
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5785..6373
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5838..5858)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6256..6267)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6460..6693
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6765..6886
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6802..6821)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6856..6875
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6926..7061
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7081..7100)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7082..7100)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7110..7127)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7110..7126)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7119..7141)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7268..7723
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7355..7374)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7565..7586
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7749..7853
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7854..7922,7923..8714)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8072..8091)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8885..9473
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9374..9393
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9627..9644
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9761..9782
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9797..9802,9803..9820,9821..9827)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9835..9851
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9840..9862
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9859..9875
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9859..9875
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9894..9914
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9896..9914
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcttgggc gctggggccg ccgcgtgcga
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat
     2641 ttttgatgat atcctgcgac gctttttttt tggcaagata gtcttgtaaa tgcgggccaa
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg
     3121 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag
     3181 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag
     3241 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt
     3301 caggtgtcgt gaaaactacc cctgagctcc ttaaggttaa cgccaccatg gactacaaag
     3361 acgatgacga caagtctaga atgatagaac agcagaagcg taagggccca gagttgccgc
     3421 tggttccagt caagcggcag cggcatgagt tgctgttggg agcggggtct ggcccaggag
     3481 ccgggcagca gcaggcgacg ccgggagcct tgctgcaagc gggacctcca agatgttcct
     3541 cccttcaagc cccaatcatg ctgctctctg gacatgaagg ggaagtctac tgctgcaagt
     3601 tccaccccaa cggatccacc ttagcatctg caggatttga ccgactgata ttactgtgga
     3661 atgtctatgg tgactgtgat aactatgcca cactgaaggg acacagtgga gcagtgatgg
     3721 aattgcatta caacacagat ggcagtatgc ttttctcagc atccacagat aaaaccgtgg
     3781 ctgtgtggga tagtgaaaca ggtgagaggg ttaaaaggct aaagggacat acttcctttg
     3841 tgaattcctg ttatccagcc aggagaggcc ctcagcttgt ctgcactggc agtgacgatg
     3901 gcacagttaa gctttgggac atccggaaga aagcagccat ccagacattt cagaacacgt
     3961 accaggtgtt agctgtgacc ttcaatgaca caagtgatca gattatttct ggtggaatag
     4021 acaatgatat caaggtctgg gacctgcgcc agaacaagct aacctacacc atgagaggcc
     4081 atgcagattc agtgactggc ctgagtttaa gttctgaagg ctcttatctt ttgtccaatg
     4141 caatggacaa tacagttcgt gtctgggatg tccggccatt tgcccccaaa gagagatgtg
     4201 taaagatatt tcaaggaaat gtgcacaact ttgaaaagaa ccttctgaga tgttcttggt
     4261 cacctgatgg aagcaaaata gcagctggct cagccgacag gtttgtttat gtgtgggata
     4321 ccacaagcag gagaatattg tataagctgc ccggccatgc tggctccatc aatgaagtgg
     4381 ctttccaccc tgatgagccc atcattatct cagcatcgag tgacaagaga ctgtatatgg
     4441 gagagattca gtgaggatcc aatattcccg ggctcgagcc atggaagctt gatatctaac
     4501 tgactgaacc ggtggtaccg atccacgcgt ctccggccta gggataacag ggtaatccgc
     4561 tagcccctct ccctcccccc cccctaacgt tactggccga agccgcttgg aataaggccg
     4621 gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc
     4681 ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa
     4741 aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag
     4801 acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg
     4861 cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg
     4921 ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa
     4981 caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg
     5041 gtacacatgc tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca
     5101 cggggacgtg gttttccttt gaaaaacacg atgataatat ggccacacta gagatccacc
     5161 ggtcgccacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
     5221 cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
     5281 cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
     5341 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac
     5401 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga
     5461 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg
     5521 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa
     5581 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
     5641 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
     5701 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc
     5761 cggtgcctga gcggccgcgt cgacaatcaa cctctggatt acaaaatttg tgaaagattg
     5821 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     5881 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     5941 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     6001 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     6061 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     6121 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag
     6181 ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     6241 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     6301 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     6361 gccgcctccc cgcctggaat tcgagctcgg tacctttaag accaatgact tacaaggcag
     6421 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     6481 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
     6541 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     6601 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     6661 gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc
     6721 agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag
     6781 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     6841 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct
     6901 agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     6961 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     7021 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg
     7081 ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg
     7141 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     7201 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     7261 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     7321 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     7381 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     7441 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     7501 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     7561 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     7621 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     7681 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg
     7741 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     7801 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     7861 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     7921 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     7981 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     8041 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     8101 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     8161 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     8221 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     8281 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     8341 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     8401 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     8461 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     8521 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     8581 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     8641 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     8701 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     8761 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     8821 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     8881 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     8941 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     9001 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     9061 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     9121 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     9181 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     9241 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     9301 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     9361 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     9421 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     9481 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     9541 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     9601 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     9661 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     9721 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     9781 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     9841 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta
     9901 accctcacta aagggaacaa aagctggagc tgc
//
