LOCUS       sequence-27537-e         692 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Evers TH, van Dongen EM, Faesen AC, Meijer EW, Merkx M
  TITLE     Quantitative understanding of the energy transfer between 
            fluorescent proteins connected via flexible peptide linkers.
  JOURNAL   Biochemistry. 2006 Nov 7. 45(44):13183-92.
  PUBMED    17073440
REFERENCE   2  (bases 1 to 692)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2006 Nov 7. 45(44):13183-92."
COMMENT     Sequence Label: pET28CLY2
FEATURES             Location/Qualifiers
     source          1..692
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     100..121
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(260..281)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     609..628
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 cgggcagctt gccggtggtg cagatgaact tcagggtcag cttgccgtag gtggcatcgc
       61 cctcgccctc gccggacacg ctgaacttgt ggccgtttac gtcgccgtcc agctcgacca
      121 ggatgggcac caccccggtg aacagctcct cgcccttgct caccatggtg ctacctccgg
      181 atccaccact accaccggat ccaccacgaa ttccggactt gtacagctcg tccatgccga
      241 gagtgatccc ggcggcggtc acgaactcca gcaggaccat gtgatcgcgc ttctcgttgg
      301 ggtctttgct cagggcggac tgggtgctca ggtagtggtt gtcgggcagc agcacggggc
      361 cgtcgccgat gggggtgttc tgctggtagt ggtcggcgag ctgcacgctg ccgtcctcga
      421 tgttgtggcg gatcttgaag ttggccttga tgccgttctt ctgcttgtcg gcggtgatat
      481 agacgttgtg gctgatgtag ttgtactcca gcttgtgccc caggatgttg ccgtcctcct
      541 tgaagtcgat gcccttcagc tcgatgcggt tcaccagggt gtcgccctcg aacttcacct
      601 cggcgcgggt cttgtagttg ccgtcgtcct tgaagaagat ggtgcgctcc tggacgtagc
      661 cttcgggcat ggcggacttg aagaagtcgt gc
//
