LOCUS       sequence-275373-        9816 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivector encoding Flag-tagged skywarp form of Snrnp40 (missing 
            exon 5).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9816)
  AUTHORS   Zhang D, Yue T, Choi JH, Nair-Gill E, Zhong X, Wang KW, Zhan X, Li 
            X, Choi M, Tang M, Quan J, Hildebrand S, Moresco EMY, Beutler B
  TITLE     Syndromic immune disorder caused by a viable hypomorphic allele of 
            spliceosome component Snrnp40.
  JOURNAL   Nat Immunol. 2019 Oct;20(10):1322-1334. doi: 
            10.1038/s41590-019-0464-4. Epub 2019 Aug 19.
  PUBMED    31427773
REFERENCE   2  (bases 1 to 9816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41590-019-0464-4"; journalName: "Nat Immunol"; date: 
            "2019-10"; volume: "20"; issue: "10"; pages: "1322-1334"
COMMENT     Sequence Label: pBOB-N-Flag-Snrnp40-skywarp
FEATURES             Location/Qualifiers
     source          1..9816
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1059..1184
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1677..1910
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2095..2139
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2391..2507
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2530..2833
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2834..3037
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3034..3058
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      3126..3135
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3135..3158
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     intron          4217..4446
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     4429..4448
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    4623..5211
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4676..4696)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5094..5105)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5618..5798
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5824..5841)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5830..6054
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6100..6528
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6187..6206)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6397..6418
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6537..6557)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6542..6871
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6722..6857
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6784..6803
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6919..6966
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6985..7359
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7489..7610
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7526..7545)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7580..7599
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7659..7675)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7659..7675)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7672..7694)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7683..7699
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7707..7713,7714..7731,7732..7737))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7752..7773
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7890..7907)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8061..8649)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8141..8160)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8820..9611,9612..9680))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9443..9462
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9681..9785)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     1081 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     1141 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     1201 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     1261 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     1321 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     1381 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     1441 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     1501 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     1561 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1621 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1681 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1741 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1801 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1861 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1921 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1981 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     2041 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     2101 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     2161 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     2221 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     2281 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     2341 ggtggagaga gagacagaga cagatccatt cgattagtga acggatcaac ttttaaaaga
     2401 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
     2461 atacaaacta aagaattaca aaaacaaatt acaaaattca aaattttatc gataagcttg
     2521 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     2581 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     2641 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     2701 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     2761 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     2821 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
     2881 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     2941 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
     3001 taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc
     3061 ctggagacgc catccacgct gttttgacct ccatagaaga caccgactga gctcctaagg
     3121 ttaacgccac catggactac aaagacgatg acgacaagtc tagagaattc ggatccatga
     3181 tcgagcagca gaagcgtaag ggcccggagc tacccctggt tcccgtcaag cggccgcgac
     3241 atgagttgct gttgggagcg gcaggagccg gccccggagc ggggccgcag caggcgactc
     3301 caggggcatt gctgcaggcg ggaccgccca ggtgttcttc ccttcaagct ccaatcatgc
     3361 tgctctcggg acatgagggg gaagtgtact gctgcaagtt tcaccccaat ggatccaccc
     3421 tggcttctgc aggatttgac cggctaatac tgttgtggaa cgtctatgga gactgtgata
     3481 actatgcgac gttgaaggga cacagtggag cagtgatgga gctgcactat aacacagatg
     3541 gcagcatgct cttctcagcg tcgacagaca aaactgtggc ggtgtgggat agtgaaactg
     3601 gggagagagt gaaaaggcta aaggggcata cttcctttgt gaactcctgt tacccagcca
     3661 ggcggggccc ccaacttgtc tgtactggca gtgacgatgg tacagttaag gtctgggacc
     3721 tacgccaaaa caagttaacc tacaccatga gaggccatgc agactctgta actggcctga
     3781 gcttgagttc tgaaggctct tatctcctgt ccaatgcaat ggacaacaca gttcgtgtct
     3841 gggatgtccg gccatttgct cccaaagaga gatgtgtgaa gatatttcaa ggaaatgtcc
     3901 acaactttga aaagaacctc ctgagatgtt cctggtctcc agatggaagc aagatagcag
     3961 ctggctcagc agacaggttt gtgtacgtgt gggacaccac aagccgaagg gtcctgtaca
     4021 agctgcctgg tcacgctggc tccatcaatg aagtggcttt ccaccccgac gagcccatca
     4081 tcctctcagc atccagtgac aagaggctgt acatgggtga gatccagtga ctcgagccat
     4141 ggaagcttga tatctaactg actgaaccgg tggtaccgag gaattaattc gctgtctgcg
     4201 agggccagct gttggggtga gtactccctc tcaaaagcgg gcatgacttc tgcgctaaga
     4261 ttgtcagttt ccaaaaacga ggaggatttg atattcacct ggcccgcggt gatgcctttg
     4321 agggtggccg cgtccatctg gtcagaaaag acaatctttt tgttgtcaag cttgaggtgt
     4381 ggcaggcttg agatctggcc atacacttga gtgacaatga catccacttt gcctttctct
     4441 ccacaggtgt ccactcccag gtccaactgc aggtcgagca tgcatctagg gcggccaatt
     4501 ccgccgatcc tggaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct
     4561 gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag
     4621 acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     4681 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     4741 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     4801 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     4861 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     4921 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     4981 tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc
     5041 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     5101 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     5161 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaattc
     5221 gagctcggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     5281 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atatccttga
     5341 tctgtggatc taccacacac aaggctactt ccctgattgg cagaactaca caccagggcc
     5401 agggatcaga tatccactga cctttggatg gtgctacaag ctagtaccag ttgagcaaga
     5461 gaaggtagaa gaagccaatg aaggagagaa cacccgcttg ttacaccctg tgagcctgca
     5521 tgggatggat gacccggaga gagaagtatt agagtggagg tttgacagcc gcctagcatt
     5581 tcatcacatg gcccgagagc tgcatccgga ctgtactggg tctctctggt tagaccagat
     5641 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     5701 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     5761 cctcagaccc ttttagtcag tgtggaaaat ctctagcagg gccgctttaa acccgctgat
     5821 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     5881 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     5941 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     6001 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg
     6061 aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat
     6121 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     6181 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     6241 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     6301 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     6361 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     6421 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     6481 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa
     6541 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     6601 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     6661 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     6721 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     6781 ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg
     6841 aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt
     6901 cggatctgat cagcacgtgt tgacaattaa tcatcggcat agtatatcgg catagtataa
     6961 tacgacaagg tgaggaacta aaccatggcc aagttgacca gtgccgttcc ggtgctcacc
     7021 gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac
     7081 ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg
     7141 gtccaggacc aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg cggcctggac
     7201 gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc ctccgggccg
     7261 gccatgaccg agatcggcga gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc
     7321 ggcaactgcg tgcacttcgt ggccgaggag caggactgac acgtgctacg agatttcgat
     7381 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg
     7441 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt
     7501 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     7561 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt
     7621 ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga
     7681 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     7741 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     7801 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     7861 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     7921 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     7981 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     8041 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     8101 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     8161 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     8221 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     8281 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     8341 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     8401 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     8461 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     8521 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     8581 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     8641 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     8701 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     8761 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     8821 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     8881 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     8941 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     9001 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     9061 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     9121 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     9181 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     9241 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     9301 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     9361 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     9421 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     9481 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     9541 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     9601 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     9661 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     9721 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     9781 ccgcgcacat ttccccgaaa agtgccacct gacgtc
//
