LOCUS       sequence-275381-       10459 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivector encoding Flag-tagged GK5 (isoform 2).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10459)
  AUTHORS   Zhang D, Tomisato W, Su L, Sun L, Choi JH, Zhang Z, Wang KW, Zhan X,
            Choi M, Li X, Tang M, Castro-Perez JM, Hildebrand S, Murray AR, 
            Moresco EMY, Beutler B
  TITLE     Skin-specific regulation of SREBP processing and lipid biosynthesis 
            by glycerol kinase 5.
  JOURNAL   Proc Natl Acad Sci U S A. 2017 Jun 27;114(26):E5197-E5206. doi: 
            10.1073/pnas.1705312114. Epub 2017 Jun 12.
  PUBMED    28607088
REFERENCE   2  (bases 1 to 10459)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1705312114"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2017-06-27- 27"; volume: "114"; issue: "26"; pages: 
            "E5197-E5206"
COMMENT     Sequence Label: pBOB-C-Flag-GK5_v2
FEATURES             Location/Qualifiers
     source          1..10459
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1059..1184
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1677..1910
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2095..2139
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2391..2507
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2530..2833
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2834..3037
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3034..3058
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             4768..4791
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     intron          4860..5089
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     5072..5091
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    5266..5854
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5319..5339)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5737..5748)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6261..6441
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6467..6484)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6473..6697
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6743..7171
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6830..6849)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7040..7061
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7180..7200)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7185..7514
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7365..7500
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7427..7446
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7562..7609
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7628..8002
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8132..8253
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8169..8188)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8223..8242
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8302..8318)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8302..8318)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8315..8337)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8326..8342
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8350..8356,8357..8374,8375..8380))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8395..8416
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8533..8550)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8704..9292)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8784..8803)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9463..10254,10255..10323))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10086..10105
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10324..10428)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     1081 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     1141 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     1201 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     1261 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     1321 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     1381 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     1441 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     1501 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     1561 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1621 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1681 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1741 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1801 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1861 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1921 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1981 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     2041 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     2101 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     2161 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     2221 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     2281 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     2341 ggtggagaga gagacagaga cagatccatt cgattagtga acggatcaac ttttaaaaga
     2401 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
     2461 atacaaacta aagaattaca aaaacaaatt acaaaattca aaattttatc gataagcttg
     2521 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     2581 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     2641 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     2701 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     2761 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     2821 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
     2881 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     2941 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
     3001 taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc
     3061 ctggagacgc catccacgct gttttgacct ccatagaaga caccgactga gctccttaag
     3121 gttaactcta gagaattcgg atccatgtca gggcagcagg agcgtgcaga gcggcagcga
     3181 gaggaactga gcgcgtcggc ctcgccgccc tcccgcttcg tgctggggct ggacgtgggc
     3241 agcacggtga tccgctgcca cgtctatgac cagacggcgc gagtgcgagg ctccagcgcg
     3301 cagaaggtag aaaatgttta ccctcaacct ggctgggttg aaatcgatcc tgattctctc
     3361 tgggctcagt ttgttgccgt aataaaagat gctgtcaaag ctgcaggggt gcagatgaat
     3421 caaattgtcg gtcttggcat atcaacacag agagccactt tcattacatg gaacaagaaa
     3481 acaggccatc attttcacaa cttcattagc tggcaagacc taagggcagc tgaacttgta
     3541 aagtcctgga ataattctct cataatgaag ctattgcatg gggccacccg agtccttcat
     3601 ttcttcagta gaagtaaagt aatgctaacg gtcagccgct tcaatttcag cacccagcat
     3661 gccaccttaa gattgacctg gattttacaa aacctatctg aggtgaaaag ggcagttgaa
     3721 gaagacaatt gttgctttgg gacgattgat acctggctgt tatacaagct cacgaaaggt
     3781 tcttcctacg ccacggatta ttcaaatgcc agcaccactg gattttttga tccctatgcg
     3841 atgcgctgga gcaggctcat aaccaccatg gtctccatcc cactgtctat cctgcctcct
     3901 gtgaaggata ccagctataa ttttggatca gtggatgaga agatatttgg cgtgcctata
     3961 ccagttgttg ccttggttgg tgaccagcag tcggccatgt ttggagagtg ctgcttcgag
     4021 acgggagatg tgaagctaac catgggaact ggaacatttt tggatatcaa tactggaaaa
     4081 aaccttcagc atgttaatgg aggcttttat ccactaattg gatggaagat tgggcaagaa
     4141 cttgtatgtt tagctgaagg caatgcagga gacacgggca ccgccataat gtgggctcaa
     4201 aaattagatc tcttcacaga cgctgctgag actgaaaaaa tggccctgag cctggaagat
     4261 tctgaagggg tttactttgt tccgtcgttt agtggattac aggctccatt aaatgacccc
     4321 tgtgcctgtg cctcttttat gggtttgaag cactccacta ataaatacca tcttgtccga
     4381 gcaatattgg agtcaatagc tttcaggaac aaacagttgt acgacatgct gcagagagaa
     4441 atccagattc ccgtcacgaa catccgggca gatggaggcg tgtgtaacaa cgctttcgtc
     4501 atgcagatga cttcagacct gataaatgag aagatagaca gacctgccca cttcgacatg
     4561 tcctgcttgg gggcagcctc tctggctggc ctcgctgttg gattttgggc ggacaaggaa
     4621 gagctccaga agctgaggca aagcgagatg gttttcaagc ctcagaagaa gtggcaggaa
     4681 tacgaggtga atatggaaaa ctgggtaaaa gcggtgaagc gctccatgaa ttggtataac
     4741 aagacgctcg agccatggaa gcttatggac tacaaagacg atgacgacaa ggatatctaa
     4801 ctgactgaac cggtggtacc gaggaattaa ttcgctgtct gcgagggcca gctgttgggg
     4861 tgagtactcc ctctcaaaag cgggcatgac ttctgcgcta agattgtcag tttccaaaaa
     4921 cgaggaggat ttgatattca cctggcccgc ggtgatgcct ttgagggtgg ccgcgtccat
     4981 ctggtcagaa aagacaatct ttttgttgtc aagcttgagg tgtggcaggc ttgagatctg
     5041 gccatacact tgagtgacaa tgacatccac tttgcctttc tctccacagg tgtccactcc
     5101 caggtccaac tgcaggtcga gcatgcatct agggcggcca attccgccga tcctggaaaa
     5161 acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg cctggctaga
     5221 agcacaagag gaggaggagg tgggttttcc agtcacacct cagacaatca acctctggat
     5281 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     5341 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     5401 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     5461 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     5521 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     5581 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     5641 tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc
     5701 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     5761 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     5821 acgagtcgga tctccctttg ggccgcctcc ccgcctggaa ttcgagctcg gtacctttaa
     5881 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     5941 tggaagggct aattcactcc caacgaagac aagatatcct tgatctgtgg atctaccaca
     6001 cacaaggcta cttccctgat tggcagaact acacaccagg gccagggatc agatatccac
     6061 tgacctttgg atggtgctac aagctagtac cagttgagca agagaaggta gaagaagcca
     6121 atgaaggaga gaacacccgc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
     6181 agagagaagt attagagtgg aggtttgaca gccgcctagc atttcatcac atggcccgag
     6241 agctgcatcc ggactgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     6301 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     6361 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     6421 cagtgtggaa aatctctagc agggccgctt taaacccgct gatcagcctc gactgtgcct
     6481 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     6541 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     6601 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
     6661 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc
     6721 tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     6781 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     6841 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     6901 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     6961 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     7021 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     7081 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     7141 gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt
     7201 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     7261 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     7321 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     7381 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     7441 ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     7501 taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct gatcagcacg
     7561 tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa
     7621 ctaaaccatg gccaagttga ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg
     7681 agcggtcgag ttctggaccg accggctcgg gttctcccgg gacttcgtgg aggacgactt
     7741 cgccggtgtg gtccgggacg acgtgaccct gttcatcagc gcggtccagg accaggtggt
     7801 gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg
     7861 gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga ccgagatcgg
     7921 cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact gcgtgcactt
     7981 cgtggccgag gagcaggact gacacgtgct acgagatttc gattccaccg ccgccttcta
     8041 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
     8101 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta
     8161 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     8221 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag
     8281 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     8341 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     8401 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     8461 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     8521 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     8581 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     8641 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     8701 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     8761 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     8821 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     8881 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     8941 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     9001 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     9061 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     9121 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     9181 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     9241 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     9301 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     9361 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     9421 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     9481 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     9541 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     9601 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     9661 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     9721 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     9781 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     9841 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     9901 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     9961 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
    10021 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
    10081 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
    10141 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
    10201 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
    10261 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
    10321 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
    10381 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
    10441 aaaagtgcca cctgacgtc
//
