LOCUS       sequence-275382-        9646 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivector encoding Flag-tagged GK5 truncation (amino acids 1-287).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9646)
  AUTHORS   Zhang D, Tomisato W, Su L, Sun L, Choi JH, Zhang Z, Wang KW, Zhan X,
            Choi M, Li X, Tang M, Castro-Perez JM, Hildebrand S, Murray AR, 
            Moresco EMY, Beutler B
  TITLE     Skin-specific regulation of SREBP processing and lipid biosynthesis 
            by glycerol kinase 5.
  JOURNAL   Proc Natl Acad Sci U S A. 2017 Jun 27;114(26):E5197-E5206. doi: 
            10.1073/pnas.1705312114. Epub 2017 Jun 12.
  PUBMED    28607088
REFERENCE   2  (bases 1 to 9646)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1705312114"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2017-06-27- 27"; volume: "114"; issue: "26"; pages: 
            "E5197-E5206"
COMMENT     Sequence Label: pBOB-C-Flag-GK5 (1-287)
FEATURES             Location/Qualifiers
     source          1..9646
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1059..1184
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1677..1910
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2095..2139
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2391..2507
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2530..2833
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2834..3037
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3034..3058
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             4027..4050
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     intron          4119..4348
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     4331..4350
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    4525..5113
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4578..4598)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4996..5007)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5520..5700
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5726..5743)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5732..5956
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6002..6430
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6089..6108)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6299..6320
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6439..6459)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6483..6693
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        6505..6701
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6552..6687
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6614..6633
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6749..6796
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6815..7189
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7319..7440
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7356..7375)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7410..7429
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7489..7505)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7489..7505)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7502..7524)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7513..7529
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7537..7543,7544..7561,7562..7567))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7582..7603
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7720..7737)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7891..8479)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7971..7990)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8650..9441,9442..9510))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9273..9292
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9511..9615)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     1081 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     1141 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     1201 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     1261 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     1321 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     1381 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     1441 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     1501 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     1561 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1621 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1681 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1741 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1801 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1861 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1921 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1981 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     2041 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     2101 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     2161 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     2221 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     2281 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     2341 ggtggagaga gagacagaga cagatccatt cgattagtga acggatcaac ttttaaaaga
     2401 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
     2461 atacaaacta aagaattaca aaaacaaatt acaaaattca aaattttatc gataagcttg
     2521 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     2581 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     2641 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     2701 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     2761 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     2821 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
     2881 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     2941 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
     3001 taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc
     3061 ctggagacgc catccacgct gttttgacct ccatagaaga caccgactga gctccttaag
     3121 gttaactcta gagaattcgg atccatgtca gggcagcagg agcgtgcaga gcggcagcga
     3181 gaggaactga gcgcgtcggc ctcgccgccc tcccgcttcg tgctggggct ggacgtgggc
     3241 agcacggtga tccgctgcca cgtctatgac cagacggcgc gagtgcgagg ctccagcgcg
     3301 cagaaggtag aaaatgttta ccctcaacct ggctgggttg aaatcgatcc tgattctctc
     3361 tgggctcagt ttgttgccgt aataaaagat gctgtcaaag ctgcaggggt gcagatgaat
     3421 caaattgtcg gtcttggcat atcaacacag agagccactt tcattacatg gaacaagaaa
     3481 acaggccatc attttcacaa cttcattagc tggcaagacc taagggcagc tgaacttgta
     3541 aagtcctgga ataattctct cataatgaag ctattgcatg gggccacccg agtccttcat
     3601 ttcttcagta gaagtaaagt aatgctaacg gtcagccgct tcaatttcag cacccagcat
     3661 gccaccttaa gattgacctg gattttacaa aacctatctg aggtgaaaag ggcagttgaa
     3721 gaagacaatt gttgctttgg gacgattgat acctggctgt tatacaagct cacgaaaggt
     3781 tcttcctacg ccacggatta ttcaaatgcc agcaccactg gattttttga tccctatgcg
     3841 atgcgctgga gcaggctcat aaccaccatg gtctccatcc cactgtctat cctgcctcct
     3901 gtgaaggata ccagctataa ttttggatca gtggatgaga agatatttgg cgtgcctata
     3961 ccagttgttg ccttggttgg tgaccagcag tcggccatgt ttggactcga gccatggaag
     4021 cttatggact acaaagacga tgacgacaag gatatctaac tgactgaacc ggtggtaccg
     4081 aggaattaat tcgctgtctg cgagggccag ctgttggggt gagtactccc tctcaaaagc
     4141 gggcatgact tctgcgctaa gattgtcagt ttccaaaaac gaggaggatt tgatattcac
     4201 ctggcccgcg gtgatgcctt tgagggtggc cgcgtccatc tggtcagaaa agacaatctt
     4261 tttgttgtca agcttgaggt gtggcaggct tgagatctgg ccatacactt gagtgacaat
     4321 gacatccact ttgcctttct ctccacaggt gtccactccc aggtccaact gcaggtcgag
     4381 catgcatcta gggcggccaa ttccgccgat cctggaaaaa catggagcaa tcacaagtag
     4441 caatacagca gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt
     4501 gggttttcca gtcacacctc agacaatcaa cctctggatt acaaaatttg tgaaagattg
     4561 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     4621 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     4681 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     4741 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     4801 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     4861 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag
     4921 ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     4981 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     5041 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     5101 gccgcctccc cgcctggaat tcgagctcgg tacctttaag accaatgact tacaaggcag
     5161 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     5221 aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt
     5281 ggcagaacta cacaccaggg ccagggatca gatatccact gacctttgga tggtgctaca
     5341 agctagtacc agttgagcaa gagaaggtag aagaagccaa tgaaggagag aacacccgct
     5401 tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagta ttagagtgga
     5461 ggtttgacag ccgcctagca tttcatcaca tggcccgaga gctgcatccg gactgtactg
     5521 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     5581 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     5641 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     5701 gggccgcttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     5761 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     5821 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     5881 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     5941 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc
     6001 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     6061 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     6121 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     6181 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     6241 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     6301 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     6361 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     6421 aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
     6481 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     6541 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     6601 gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga
     6661 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta
     6721 tatccatttt cggatctgat cagcacgtgt tgacaattaa tcatcggcat agtatatcgg
     6781 catagtataa tacgacaagg tgaggaacta aaccatggcc aagttgacca gtgccgttcc
     6841 ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt
     6901 ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt
     6961 catcagcgcg gtccaggacc aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg
     7021 cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc
     7081 ctccgggccg gccatgaccg agatcggcga gcagccgtgg gggcgggagt tcgccctgcg
     7141 cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag caggactgac acgtgctacg
     7201 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
     7261 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa
     7321 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     7381 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     7441 tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt
     7501 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     7561 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     7621 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     7681 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     7741 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     7801 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     7861 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     7921 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     7981 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     8041 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     8101 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     8161 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     8221 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     8281 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     8341 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     8401 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     8461 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     8521 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     8581 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     8641 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     8701 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     8761 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     8821 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     8881 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     8941 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     9001 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     9061 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     9121 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     9181 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     9241 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     9301 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     9361 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     9421 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     9481 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     9541 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     9601 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtc
//
