LOCUS       sequence-27539-e         648 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 648)
  AUTHORS   Evers TH, van Dongen EM, Faesen AC, Meijer EW, Merkx M
  TITLE     Quantitative understanding of the energy transfer between 
            fluorescent proteins connected via flexible peptide linkers.
  JOURNAL   Biochemistry. 2006 Nov 7. 45(44):13183-92.
  PUBMED    17073440
REFERENCE   2  (bases 1 to 648)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2006 Nov 7. 45(44):13183-92."
COMMENT     Sequence Label: pET28CLY3
FEATURES             Location/Qualifiers
     source          1..648
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     100..121
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(278..299)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     627..646
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 cgggcagctt gccggtggtg cagatgaact tcagggtcag cttgccgtag gtggcatcgc
       61 cctcgccctc gccggacacg ctgaacttgt ggccgtttac gtcgccgtcc agctcgacca
      121 ggatgggcac caccccggtg aacagctcct cgcccttgct caccatggtg ctacctccgg
      181 atccaccact accaccggat ccaccagatc caccggatcc accacgaatt ccggacttgt
      241 acagctcgtc catgccgaga gtgatcccgg cggcggtcac gaactccagc aggaccatgt
      301 gatcgcgctt ctcgttgggg tctttgctca gggcggactg ggtgctcagg tagtggttgt
      361 cgggcagcag cacggggccg tcgccgatgg gggtgttctg ctggtagtgg tcggcgagct
      421 gcacgctgcc gtcctcgatg ttgtggcgga tcttgaagtt ggccttgatg ccgttcttct
      481 gcttgtcggc ggtgatatag acgttgtggc tgatgtagtt gtactccagc ttgtgcccca
      541 ggatgttgcc gtcctccttg aagtcgatgc ccttcagctc gatgcggttc accagggtgt
      601 cgccctcgaa cttcacctcg gcgcgggtct tgtagttgcc gtcgtcta
//
