LOCUS       sequence-275398-        9627 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CMV and T7 promoter expression plasmid for human codon optimized 
            ABEmax(7.10) A-to-G base editor with 
            xCas9(3.7)(D10A/A262T/R324L/S409I/E480K/E543D/M694I/E1219V) and 
            P2A-EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9627)
  AUTHORS   Walton RT, Christie KA, Whittaker MN, Kleinstiver BP
  TITLE     Unconstrained genome targeting with near-PAMless engineered 
            CRISPR-Cas9 variants.
  JOURNAL   Science. 2020 Apr 17;368(6488):290-296. doi: 
            10.1126/science.aba8853. Epub 2020 Mar 26.
  PUBMED    32217751
REFERENCE   2  (bases 1 to 9627)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aba8853"; journalName: "Science"; date: 
            "2020-04-17- 17"; volume: "368"; issue: "6488"; pages: "290-296"
COMMENT     Sequence Label: pCMV-T7-ABEmax(7.10)-xCas9(3.7)-P2A-EGFP (RTW4...
FEATURES             Location/Qualifiers
     source          1..9627
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     512..531
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(750..854)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(933..952)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1124..1503
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1504..1707
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1658..1678
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1749..1768
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1749..1767
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1817..1837
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3026..7126
                     /codon_start=1
                     /product="Streptococcus pyogenes Cas9 mutant with broad PAM
                     compatibility (Hu et al., 2018)"
                     /label=xCas9-3.7
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDTKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKLYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGIIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEKVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGDQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFIQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGVLQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             3026..7126
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDTKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKLYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGIIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEKVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGDQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFIQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGVLQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             7169..7189
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7199..7255
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(7256..7258,7259..7261,7262..7972)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7256..7258,7259..7261,7262..7972)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7562..7581)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7909..7930
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             7985..8005
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8014..8031
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(8054..8071)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8060..8284
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8355..8371)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8355..8371)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8368..8390)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8379..8395
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8403..8409,8410..8427,8428..8433))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8448..8469
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8586..8603)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8757..9345)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8837..8856)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9516..9627,1..680,681..749))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
       61 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
      121 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
      181 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
      241 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
      301 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
      361 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
      421 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
      481 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
      541 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
      601 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
      661 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
      721 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
      781 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
      841 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat
      901 cgatctcccg atcccctagg gtcgactctc agtacaatct gctctgatgc cgcatagtta
      961 agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt
     1021 taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg
     1081 cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact
     1141 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
     1201 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
     1261 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
     1321 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
     1381 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
     1441 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
     1501 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
     1561 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
     1621 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
     1681 cggtgggagg tctatataag cagagctggt ttagtgaacc gtcagatccg ctagagatcc
     1741 gcggccgcta atacgactca ctatagggag agccgccacc atgaaacgga cagccgacgg
     1801 aagcgagttc gagtcaccaa agaagaagcg gaaagtctct gaagtcgagt ttagccacga
     1861 gtattggatg aggcacgcac tgaccctggc aaagcgagca tgggatgaaa gagaagtccc
     1921 cgtgggcgcc gtgctggtgc acaacaatag agtgatcgga gagggatgga acaggccaat
     1981 cggccgccac gaccctaccg cacacgcaga gatcatggca ctgaggcagg gaggcctggt
     2041 catgcagaat taccgcctga tcgatgccac cctgtatgtg acactggagc catgcgtgat
     2101 gtgcgcagga gcaatgatcc acagcaggat cggaagagtg gtgttcggag cacgggacgc
     2161 caagaccggc gcagcaggct ccctgatgga tgtgctgcac caccccggca tgaaccaccg
     2221 ggtggagatc acagagggaa tcctggcaga cgagtgcgcc gccctgctga gcgatttctt
     2281 tagaatgcgg agacaggaga tcaaggccca gaagaaggca cagagctcca ccgactctgg
     2341 aggatctagc ggaggatcct ctggaagcga gacaccaggc acaagcgagt ccgccacacc
     2401 agagagctcc ggcggctcct ccggaggatc ctctgaggtg gagttttccc acgagtactg
     2461 gatgagacat gccctgaccc tggccaagag ggcacgcgat gagagggagg tgcctgtggg
     2521 agccgtgctg gtgctgaaca atagagtgat cggcgagggc tggaacagag ccatcggcct
     2581 gcacgaccca acagcccatg ccgaaattat ggccctgaga cagggcggcc tggtcatgca
     2641 gaactacaga ctgattgacg ccaccctgta cgtgacattc gagccttgcg tgatgtgcgc
     2701 cggcgccatg atccactcta ggatcggccg cgtggtgttt ggcgtgagga acgcaaaaac
     2761 cggcgccgca ggctccctga tggacgtgct gcactacccc ggcatgaatc accgcgtcga
     2821 aattaccgag ggaatcctgg cagatgaatg tgccgccctg ctgtgctatt tctttcggat
     2881 gcctagacag gtgttcaatg ctcagaagaa ggcccagagc tccaccgact ccggaggatc
     2941 tagcggaggc tcctctggct ctgagacacc tggcacaagc gagagcgcaa cacctgaaag
     3001 cagcgggggc agcagcgggg ggtcagacaa gaagtacagc atcggcctgg ccatcggcac
     3061 caactctgtg ggctgggccg tgatcaccga cgagtacaag gtgcccagca agaaattcaa
     3121 ggtgctgggc aacaccgacc ggcacagcat caagaagaac ctgatcggag ccctgctgtt
     3181 cgacagcggc gaaacagccg aggccacccg gctgaagaga accgccagaa gaagatacac
     3241 cagacggaag aaccggatct gctatctgca agagatcttc agcaacgaga tggccaaggt
     3301 ggacgacagc ttcttccaca gactggaaga gtccttcctg gtggaagagg ataagaagca
     3361 cgagcggcac cccatcttcg gcaacatcgt ggacgaggtg gcctaccacg agaagtaccc
     3421 caccatctac cacctgagaa agaaactggt ggacagcacc gacaaggccg acctgcggct
     3481 gatctatctg gccctggccc acatgatcaa gttccggggc cacttcctga tcgagggcga
     3541 cctgaacccc gacaacagcg acgtggacaa gctgttcatc cagctggtgc agacctacaa
     3601 ccagctgttc gaggaaaacc ccatcaacgc cagcggcgtg gacgccaagg ccatcctgtc
     3661 tgccagactg agcaagagca gacggctgga aaatctgatc gcccagctgc ccggcgagaa
     3721 gaagaatggc ctgttcggaa acctgattgc cctgagcctg ggcctgaccc ccaacttcaa
     3781 gagcaacttc gacctggccg aggataccaa actgcagctg agcaaggaca cctacgacga
     3841 cgacctggac aacctgctgg cccagatcgg cgaccagtac gccgacctgt ttctggccgc
     3901 caagaacctg tccgacgcca tcctgctgag cgacatcctg agagtgaaca ccgagatcac
     3961 caaggccccc ctgagcgcct ctatgatcaa gctgtacgac gagcaccacc aggacctgac
     4021 cctgctgaaa gctctcgtgc ggcagcagct gcctgagaag tacaaagaga ttttcttcga
     4081 ccagagcaag aacggctacg ccggctacat tgacggcgga gccagccagg aagagttcta
     4141 caagttcatc aagcccatcc tggaaaagat ggacggcacc gaggaactgc tcgtgaagct
     4201 gaacagagag gacctgctgc ggaagcagcg gaccttcgac aacggcatca tcccccacca
     4261 gatccacctg ggagagctgc acgccattct gcggcggcag gaagattttt acccattcct
     4321 gaaggacaac cgggaaaaga tcgagaagat cctgaccttc cgcatcccct actacgtggg
     4381 ccctctggcc aggggaaaca gcagattcgc ctggatgacc agaaagagcg aggaaaccat
     4441 caccccctgg aacttcgaga aggtggtgga caagggcgct tccgcccaga gcttcatcga
     4501 gcggatgacc aacttcgata agaacctgcc caacgagaag gtgctgccca agcacagcct
     4561 gctgtacgag tacttcaccg tgtataacga gctgaccaaa gtgaaatacg tgaccgaggg
     4621 aatgagaaag cccgccttcc tgagcggcga ccagaaaaag gccatcgtgg acctgctgtt
     4681 caagaccaac cggaaagtga ccgtgaagca gctgaaagag gactacttca agaaaatcga
     4741 gtgcttcgac tccgtggaaa tctccggcgt ggaagatcgg ttcaacgcct ccctgggcac
     4801 ataccacgat ctgctgaaaa ttatcaagga caaggacttc ctggacaatg aggaaaacga
     4861 ggacattctg gaagatatcg tgctgaccct gacactgttt gaggacagag agatgatcga
     4921 ggaacggctg aaaacctatg cccacctgtt cgacgacaaa gtgatgaagc agctgaagcg
     4981 gcggagatac accggctggg gcaggctgag ccggaagctg atcaacggca tccgggacaa
     5041 gcagtccggc aagacaatcc tggatttcct gaagtccgac ggcttcgcca acagaaactt
     5101 catccagctg atccacgacg acagcctgac ctttaaagag gacatccaga aagcccaggt
     5161 gtccggccag ggcgatagcc tgcacgagca cattgccaat ctggccggca gccccgccat
     5221 taagaagggc atcctgcaga cagtgaaggt ggtggacgag ctcgtgaaag tgatgggccg
     5281 gcacaagccc gagaacatcg tgatcgaaat ggccagagag aaccagacca cccagaaggg
     5341 acagaagaac agccgcgaga gaatgaagcg gatcgaagag ggcatcaaag agctgggcag
     5401 ccagatcctg aaagaacacc ccgtggaaaa cacccagctg cagaacgaga agctgtacct
     5461 gtactacctg cagaatgggc gggatatgta cgtggaccag gaactggaca tcaaccggct
     5521 gtccgactac gatgtggacc atatcgtgcc tcagagcttt ctgaaggacg actccatcga
     5581 caacaaggtg ctgaccagaa gcgacaagaa ccggggcaag agcgacaacg tgccctccga
     5641 agaggtcgtg aagaagatga agaactactg gcggcagctg ctgaacgcca agctgattac
     5701 ccagagaaag ttcgacaatc tgaccaaggc cgagagaggc ggcctgagcg aactggataa
     5761 ggccggcttc atcaagagac agctggtgga aacccggcag atcacaaagc acgtggcaca
     5821 gatcctggac tcccggatga acactaagta cgacgagaat gacaagctga tccgggaagt
     5881 gaaagtgatc accctgaagt ccaagctggt gtccgatttc cggaaggatt tccagtttta
     5941 caaagtgcgc gagatcaaca actaccacca cgcccacgac gcctacctga acgccgtcgt
     6001 gggaaccgcc ctgatcaaaa agtaccctaa gctggaaagc gagttcgtgt acggcgacta
     6061 caaggtgtac gacgtgcgga agatgatcgc caagagcgag caggaaatcg gcaaggctac
     6121 cgccaagtac ttcttctaca gcaacatcat gaactttttc aagaccgaga ttaccctggc
     6181 caacggcgag atccggaagc ggcctctgat cgagacaaac ggcgaaaccg gggagatcgt
     6241 gtgggataag ggccgggatt ttgccaccgt gcggaaagtg ctgagcatgc cccaagtgaa
     6301 tatcgtgaaa aagaccgagg tgcagacagg cggcttcagc aaagagtcta tcctgcccaa
     6361 gaggaacagc gataagctga tcgccagaaa gaaggactgg gaccctaaga agtacggcgg
     6421 cttcgacagc cccaccgtgg cctattctgt gctggtggtg gccaaagtgg aaaagggcaa
     6481 gtccaagaaa ctgaagagtg tgaaagagct gctggggatc accatcatgg aaagaagcag
     6541 cttcgagaag aatcccatcg actttctgga agccaagggc tacaaagaag tgaaaaagga
     6601 cctgatcatc aagctgccta agtactccct gttcgagctg gaaaacggcc ggaagagaat
     6661 gctggcctct gccggcgtgc tgcagaaggg aaacgaactg gccctgccct ccaaatatgt
     6721 gaacttcctg tacctggcca gccactatga gaagctgaag ggctcccccg aggataatga
     6781 gcagaaacag ctgtttgtgg aacagcacaa gcactacctg gacgagatca tcgagcagat
     6841 cagcgagttc tccaagagag tgatcctggc cgacgctaat ctggacaaag tgctgtccgc
     6901 ctacaacaag caccgggata agcccatcag agagcaggcc gagaatatca tccacctgtt
     6961 taccctgacc aatctgggag cccctgccgc cttcaagtac tttgacacca ccatcgaccg
     7021 gaagaggtac accagcacca aagaggtgct ggacgccacc ctgatccacc agagcatcac
     7081 cggcctgtac gagacacgga tcgacctgtc tcagctggga ggtgactctg gcggctcaaa
     7141 aagaaccgcc gacggcagcg aattcgagcc caagaagaag aggaaagtcg gaagcggagc
     7201 tactaacttc agcctgctga agcaggctgg agacgtggag gagaaccctg gacctatggt
     7261 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     7321 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
     7381 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     7441 gaccaccctg acctatggag tgcagtgctt cagccgctac cccgaccaca tgaagcagca
     7501 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     7561 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     7621 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     7681 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat
     7741 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
     7801 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     7861 gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     7921 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtctggtgg
     7981 ttctcccaag aagaagagga aagtctaacc ggtcatcatc accatcacca ttgagtttaa
     8041 acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     8101 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     8161 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     8221 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
     8281 atggcttctg aggcggaaag aaccagctgg ggctcgatac cgtcgacctc tagctagagc
     8341 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     8401 cacaacatac gagccggaag cataaagtgt aaagcctagg gtgcctaatg agtgagctaa
     8461 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     8521 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     8581 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     8641 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     8701 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     8761 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     8821 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     8881 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     8941 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     9001 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     9061 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     9121 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     9181 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     9241 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     9301 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     9361 ttttctacgg ggtctgacac tcagtggaac gaaaactcac gttaagggat tttggtcatg
     9421 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     9481 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     9541 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     9601 ataactacga tacgggaggg cttacca
//
