LOCUS       sequence-275400-       12364 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses PguCas13b in mammalian cells, localized in the nucleus. 
            For cloning of guide RNAs compatible with PguCas13b. Contains a 3' 
            direct repeat. Clone using BsmBI. F overhang cacc. R overhang caac.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12364)
  AUTHORS   Wessels HH, Mendez-Mancilla A, Guo X, Legut M, Daniloski Z, Sanjana 
            NE
  TITLE     Massively parallel Cas13 screens reveal principles for guide RNA 
            design.
  JOURNAL   Nat Biotechnol. 2020 Jun;38(6):722-727. doi: 
            10.1038/s41587-020-0456-9. Epub 2020 Mar 16.
  PUBMED    32518401
REFERENCE   2  (bases 1 to 12364)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0456-9"; journalName: "Nat Biotechnol"; date: 
            "2020-06"; volume: "38"; issue: "6"; pages: "722-727"
COMMENT     Sequence Label: pLentiRNACRISPR_001 -
            hU6-DR_BsmBI-EFS-PguCas1...
FEATURES             Location/Qualifiers
     source          1..12364
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..580
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             598..778
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    825..950
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1443..1676
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1861..1905
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2203..2320
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2371..2619
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2371..2391
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2542..2561
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     CDS             complement(2641..2652)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        2732..2943
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             6493..6540
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             6550..6606
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6607..7203
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     7100..7120
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7219..7807
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7272..7292)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7690..7701)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7879..8112
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8138..8155)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8144..8368
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8414..8842
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8501..8520)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8711..8732
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8851..8871)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8856..9185
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9036..9171
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9098..9117
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9233..9280
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9299..9673
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9803..9924
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9840..9859)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9894..9913
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9973..9989)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9973..9989)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9986..10008)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9997..10013
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10021..10027,10028..10045,10046..10051))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10066..10087
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10204..10221)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10375..10963)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10455..10474)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11134..11925,11926..11994))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11757..11776
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11995..12099)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(12174..12193)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
      601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1261 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     1321 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1381 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1441 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1501 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1561 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1621 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1681 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1741 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     1801 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     1861 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     1921 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     1981 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2041 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2101 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     2161 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     2221 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     2281 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     2341 cagagatcca gtttggttaa ttaaggtacc gagggcctat ttcccatgat tccttcatat
     2401 ttgcatatac gatacaaggc tgttagagag ataattggaa ttaatttgac tgtaaacaca
     2461 aagatattag tacaaaatac gtgacgtaga aagtaataat ttcttgggta gtttgcagtt
     2521 ttaaaattat gttttaaaat ggactatcat atgcttaccg taacttgaaa gtatttcgat
     2581 ttcttggctt tatatatctt gtggaaagga cgaaacaccg agacggacgt ctctgttgga
     2641 tctaccctct atttgaaggg tacacacaac ttttttgaat tcgctagcta ggtcttgaaa
     2701 ggagtgggaa ttggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc
     2761 gagaagttgg ggggaggggt cggcaattga tccggtgcct agagaaggtg gcgcggggta
     2821 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg
     2881 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca
     2941 caggaccggt tctagagcgc tgccaccatg acagagcaga gcgagaggcc ctacaacggc
     3001 acctactaca ccctggaaga taagcacttc tgggccgcct ttctgaacct ggccagacac
     3061 aacgcctaca tcaccctgac acacatcgac cggcagctgg cctacagcaa ggccgacatc
     3121 accaacgacc aggacgtgct gagcttcaag gccctgtgga agaacttcga caacgacctg
     3181 gaaagaaaga gccggctgcg gagcctgatc ctgaagcact tcagctttct ggaaggcgcc
     3241 gcttacggca agaagctgtt cgagtctaag agcagcggca acaagagcag caagaacaaa
     3301 gagctgacca agaaagagaa agaggaactg caggccaacg ctctgagcct ggacaacctg
     3361 aagtctatcc tgttcgactt cctgcagaag ctgaaggact tccggaacta ctacagccac
     3421 taccggcaca gcggaagcag cgagctgcct ctgttcgacg gcaacatgct gcagcggctg
     3481 tacaacgtgt tcgacgtgtc cgtgcagcgc gtgaagatcg accacgagca caacgatgag
     3541 gtggaccctc actaccactt caaccacctc gtgcggaagg gcaagaagga cagatacggc
     3601 cacaacgaca accccagctt caagcaccac ttcgtggacg gcgaaggcat ggttacagaa
     3661 gccggcctgc tgttcttcgt gtccctgttc ctggaaaagc gggacgccat ctggatgcag
     3721 aagaagatcc ggggcttcaa aggcggcacc gagacatacc agcagatgac caacgaggtg
     3781 ttctgccggt ccagaatcag cctgcctaag ctgaagctgg aaagcctgcg gatggacgac
     3841 tggatgctgc tggacatgct gaacgaactc gtgcggtgcc ccaagcctct gtacgacaga
     3901 ctgagagagg acgaccgggc ctgctttaga gtgcccgtgg atatcctgcc tgacgaggac
     3961 gatacagatg gcggcggaga ggaccccttc aagaataccc tcgtccggca ccaggacaga
     4021 ttcccttact tcgccctgcg gtacttcgac ctgaagaagg tgttcaccag cctgcggttc
     4081 cacatcgatc tgggcaccta ccactttgcc atctacaaga agatgatcgg cgagcagccc
     4141 gaggacagac acctgaccag aaacctgtac ggcttcggcc ggatccagga cttcgccgaa
     4201 gaacacagac ccgaggaatg gaagcggctc gtcagagatc tggactactt cgagacaggc
     4261 gacaagccct acatcagcca gacaagccca cactaccaca tcgagaaggg aaagatcggc
     4321 ctgagattca tgcccgaggg ccagcatctt tggccctctc cagaagtggg caccaccaga
     4381 accggcagat ctaagtacgc ccaggacaag agactgaccg ccgaggcctt tctgtctgtg
     4441 cacgagctga tgcctatgat gttctactac ttcctgctgc gcgagaagta cagcgaggaa
     4501 gtgtctgccg agagagtgca gggcagaatc aagcgcgtga tcgaggatgt gtacgccgtg
     4561 tacgatgcct ttgccaggga cgagatcaac acccgggatg agctggatgc ctgcctggcc
     4621 gataagggca tcagaagagg ccatctgcct cgccagatga tcgccatcct gagccaagag
     4681 cacaaggaca tggaagagaa gattcggaag aaactgcaag agatgatggc cgacaccgac
     4741 caccggctgg atatgctgga tagacagacc gaccggaaga tcagaatcgg ccggaagaat
     4801 gccggactgc ctaagagcgg agtgatcgcc gattggctcg tgcgggacat gatgagattt
     4861 cagcccgtgg ccaaggacgc cagcggaaag cctctgaaca actccaaggc caacagcacc
     4921 gagtaccgga tgctgcagag agccctggct ctgtttggag gcgagaaaga gaggctgacc
     4981 ccatacttcc ggcagatgaa tctgaccggc ggaaacaacc ctcatccttt tctgcacgaa
     5041 actcgctggg agagccacac caacatcctg tccttttacc ggtcctacct gagagcccgc
     5101 aaggccttcc tcgaaaggat cggcagatcc gacagagtgg aaaaccggcc atttctgctg
     5161 ctgaaagagc ccaagaccga cagacagaca ctggtggctg gatggaaggg cgagttccat
     5221 ctgccaagag gcatcttcac agaggccgtg cgggattgcc tgatcgagat gggacacgat
     5281 gaagtggcca gctacaaaga agtgggattc atggccaagg ccgtgccact gtactttgag
     5341 agagcctgcg aggacagggt gcagcccttt tacgacagcc ccttcaacgt gggcaacagc
     5401 ctgaagccta agaagggcag attcctgagc aaagaggaac gggccgaaga gtgggagcgg
     5461 ggcaaagaga gattccggga tctcgaagcc tggtcctata gcgccgccag aagaatcgag
     5521 gacgcctttg ccggcatcga gtatgctagc cccggaaaca agaagaaaat cgagcagctc
     5581 ctgcgggacc tgagcctgtg ggaagccttt gagagcaagc tgaaagtgcg ggccgaccgg
     5641 atcaatctgg ccaagctgaa gaaagaaatc ctggaagccc aagaacaccc ctaccacgac
     5701 ttcaagagct ggcagaagtt cgagcgcgag ctgcggctgg tcaagaacca ggatatcatc
     5761 acctggatga tgtgccgcga cctgatggaa gaaaacaagg tggaaggcct ggacaccggc
     5821 acactgtacc tgaaggacat cagacccaac gtgcaagagc agggcagcct gaacgtgctg
     5881 aacagagtga agcccatgag actgcccgtg gtggtgtaca gagccgatag cagaggccac
     5941 gtgcacaaag aagaagcccc tctggccacc gtgtacatcg aggaaagaga caccaagctg
     6001 ctgaagcagg gcaacttcaa gtccttcgtg aaggacaggc ggctgaacgg cctgttctcc
     6061 ttcgttgata ctggcggact ggccatggaa cagtacccca tctccaagct gagagtggaa
     6121 tacgagctgg ccaagtacca gaccgccaga gtgtgtgtgt tcgagctgac cctgagactg
     6181 gaagagtccc tgctgacccg gtatcctcat ctgcccgacg agagcttcag agagatgctg
     6241 gaatcttgga gcgaccctct gctggccaaa tggcctgaac tgcacggaaa agtgcggctg
     6301 ctgatcgccg tgcggaatgc ctttagccac aatcagtacc ctatgtacga cgaggccgtg
     6361 ttcagctcca tccggaagta cgatcctagc agccccgacg ccatcgaaga gagaatgggc
     6421 ctgaatatcg cccaccgcct gtctgaggaa gtgaagcagg ccaaagaaac cgtggaacgg
     6481 atcattcagg ccaagcgacc tgccgccaca aagaaggctg gacaggctaa gaagaagaaa
     6541 ggatccggcg caacaaactt ctctctgctg aaacaagccg gagatgtcga agagaatcct
     6601 ggaccgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc
     6661 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg
     6721 gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc
     6781 gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg
     6841 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc
     6901 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag
     6961 gagcccgcgt ggttcctggc caccgtcgga gtctcgcccg accaccaggg caagggtctg
     7021 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc
     7081 ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc
     7141 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc
     7201 tgaacgcgtt aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     7261 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     7321 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     7381 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     7441 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     7501 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     7561 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     7621 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     7681 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     7741 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     7801 tccccgcgtc gactttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     7861 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt
     7921 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     7981 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     8041 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     8101 aaatctctag cagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc
     8161 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     8221 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     8281 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     8341 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct
     8401 agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     8461 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     8521 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     8581 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     8641 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     8701 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     8761 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     8821 taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt
     8881 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     8941 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     9001 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     9061 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     9121 cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     9181 gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcagcac gtgttgacaa
     9241 ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccat
     9301 ggccaagttg accagtgccg ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga
     9361 gttctggacc gaccggctcg ggttctcccg ggacttcgtg gaggacgact tcgccggtgt
     9421 ggtccgggac gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg tgccggacaa
     9481 caccctggcc tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt
     9541 cgtgtccacg aacttccggg acgcctccgg gccggccatg accgagatcg gcgagcagcc
     9601 gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga
     9661 ggagcaggac tgacacgtgc tacgagattt cgattccacc gccgccttct atgaaaggtt
     9721 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     9781 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag
     9841 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     9901 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt
     9961 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
    10021 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
    10081 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
    10141 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
    10201 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
    10261 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
    10321 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
    10381 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
    10441 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
    10501 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
    10561 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
    10621 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
    10681 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
    10741 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
    10801 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
    10861 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
    10921 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
    10981 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
    11041 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
    11101 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
    11161 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
    11221 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
    11281 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
    11341 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
    11401 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
    11461 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
    11521 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
    11581 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
    11641 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
    11701 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
    11761 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
    11821 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
    11881 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
    11941 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
    12001 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
    12061 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
    12121 acctgacgtc gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc
    12181 tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct
    12241 gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg
    12301 aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg
    12361 cgtt
//
