LOCUS       sequence-275415-       10521 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivector encoding Flag-tagged GK (isoform 2).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10521)
  AUTHORS   Zhang D, Tomisato W, Su L, Sun L, Choi JH, Zhang Z, Wang KW, Zhan X,
            Choi M, Li X, Tang M, Castro-Perez JM, Hildebrand S, Murray AR, 
            Moresco EMY, Beutler B
  TITLE     Skin-specific regulation of SREBP processing and lipid biosynthesis 
            by glycerol kinase 5.
  JOURNAL   Proc Natl Acad Sci U S A. 2017 Jun 27;114(26):E5197-E5206. doi: 
            10.1073/pnas.1705312114. Epub 2017 Jun 12.
  PUBMED    28607088
REFERENCE   2  (bases 1 to 10521)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1705312114"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2017-06-27- 27"; volume: "114"; issue: "26"; pages: 
            "E5197-E5206"
COMMENT     Sequence Label: pBOB-N-Flag-GK_v2
FEATURES             Location/Qualifiers
     source          1..10521
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1059..1184
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1677..1910
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2095..2139
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2391..2507
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2530..2833
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2834..3037
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3034..3058
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      3126..3135
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3135..3158
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     intron          4922..5151
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     5134..5153
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    5328..5916
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5381..5401)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5799..5810)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6323..6503
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6529..6546)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6535..6759
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6805..7233
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6892..6911)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7102..7123
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7242..7262)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7247..7576
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7427..7562
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7489..7508
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7624..7671
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7690..8064
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8194..8315
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8231..8250)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8285..8304
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8364..8380)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8364..8380)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8377..8399)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8388..8404
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8412..8418,8419..8436,8437..8442))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8457..8478
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8595..8612)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8766..9354)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8846..8865)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9525..10316,10317..10385))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10148..10167
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10386..10490)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     1081 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     1141 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     1201 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     1261 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     1321 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     1381 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     1441 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     1501 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     1561 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1621 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1681 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1741 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1801 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1861 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1921 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1981 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     2041 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     2101 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     2161 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     2221 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     2281 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     2341 ggtggagaga gagacagaga cagatccatt cgattagtga acggatcaac ttttaaaaga
     2401 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
     2461 atacaaacta aagaattaca aaaacaaatt acaaaattca aaattttatc gataagcttg
     2521 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     2581 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     2641 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     2701 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     2761 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     2821 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
     2881 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     2941 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
     3001 taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc
     3061 ctggagacgc catccacgct gttttgacct ccatagaaga caccgactga gctcctaagg
     3121 ttaacgccac catggactac aaagacgatg acgacaagtc tagagaattc ggatccatgg
     3181 cagccgcgaa gaaagcagtt ctggggccat tggtgggagc agtggaccag ggtaccagct
     3241 cgacacgttt tttggttttc aattcaaaaa cagctgaact tcttagtcat catcaagtag
     3301 aaataaaaca ggaattccca agagaaggat gggtagaaca agacccgaag gaaattctgc
     3361 agtctgttta tgagtgtata gagaaaacgt gtgagaaact tggacagctc aatattgata
     3421 tttccaacat caaagccatt ggtgtcagca accagaggga aaccacagta gtctgggaca
     3481 aggtcaccgg agagcctctc tataatgccg tggtgtggct tgacctaaga acccagtcta
     3541 ctgttgagaa ccttagtaaa agaattccag gaaataataa ctttgtcaag tccaagacag
     3601 gccttccact tagcacgtat ttcagtgcag tgaaacttcg ttggctcctt gacaacgtga
     3661 aaaaggtcca agaggctgtt gaagaaaata gagctctttt tgggaccatt gattcatggc
     3721 ttatttggag tttaacagga ggaatccatg ggggtgtcca ctgtacagat gtaacaaatg
     3781 caagcaggac gatgcttttt aacattcatt ctttggaatg ggataaagag ctctgcgaat
     3841 tttttggaat tccaatggaa attcttccca acgttcggag ttcttctgag atctatggcc
     3901 taatgaaagc tggggccttg gaaggtgtac caatatctgg gtgtttgggg gaccagtctg
     3961 ctgctttggt gggacaaatg tgcttccagg atggacaggc caaaaacacg tatggaacag
     4021 ggtgcttctt attgtgcaac acgggccata agtgtgtatt ttctgaacat ggcctcctga
     4081 caaccgtagc atataaactt ggcagagaca aacctgtgta ttatgcgttg gaaggttccg
     4141 tggctatagc tggtgctgta atccgctggc taagagacaa ccttggaatt attaagtcct
     4201 ctgaggaaat tgaaaaactt gctaaggaag taggtacttc ttatggctgc tacttcgttc
     4261 cagcattttc agggttatat gcgccttatt gggagcccag tgcaagaggg atcatctgtg
     4321 gactcactca gttcaccaat aaatgtcata tcgcttttgc tgcactagaa gctgtttgtt
     4381 tccaaacccg agagattttg gatgccatga atcgcgactg tggaattcca ctcagtcatt
     4441 tacaggtaga tggaggaatg accagcaata aaattcttat gcagctacaa gcagacattc
     4501 tgtatattcc agtagtgaaa ccctccatgc ctgaaacaac tgcactaggc gctgccatgg
     4561 cagctggggc tgcagagggg gttggtgtgt ggagtcttga acctgaggat ttgtcagctg
     4621 tcacaatgga gcggtttgaa cctcagatca atgctgaaga aagcgaaatc cgttactcca
     4681 catggaagaa agctgtgatg aagtcaattg gttgggttac aactcagtct ccagaaagtg
     4741 gtgaccctag tatcttctgt agtctgcctt tgggcttttt tatagtgagt agcatggtaa
     4801 tgttaatcgg agcaaggtac atctcaggta tcccactcga gccatggaag cttgatatct
     4861 aactgactga accggtggta ccgaggaatt aattcgctgt ctgcgagggc cagctgttgg
     4921 ggtgagtact ccctctcaaa agcgggcatg acttctgcgc taagattgtc agtttccaaa
     4981 aacgaggagg atttgatatt cacctggccc gcggtgatgc ctttgagggt ggccgcgtcc
     5041 atctggtcag aaaagacaat ctttttgttg tcaagcttga ggtgtggcag gcttgagatc
     5101 tggccataca cttgagtgac aatgacatcc actttgcctt tctctccaca ggtgtccact
     5161 cccaggtcca actgcaggtc gagcatgcat ctagggcggc caattccgcc gatcctggaa
     5221 aaacatggag caatcacaag tagcaataca gcagctacca atgctgattg tgcctggcta
     5281 gaagcacaag aggaggagga ggtgggtttt ccagtcacac ctcagacaat caacctctgg
     5341 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     5401 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     5461 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     5521 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     5581 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     5641 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     5701 attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca
     5761 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     5821 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     5881 agacgagtcg gatctccctt tgggccgcct ccccgcctgg aattcgagct cggtaccttt
     5941 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     6001 actggaaggg ctaattcact cccaacgaag acaagatatc cttgatctgt ggatctacca
     6061 cacacaaggc tacttccctg attggcagaa ctacacacca gggccaggga tcagatatcc
     6121 actgaccttt ggatggtgct acaagctagt accagttgag caagagaagg tagaagaagc
     6181 caatgaagga gagaacaccc gcttgttaca ccctgtgagc ctgcatggga tggatgaccc
     6241 ggagagagaa gtattagagt ggaggtttga cagccgccta gcatttcatc acatggcccg
     6301 agagctgcat ccggactgta ctgggtctct ctggttagac cagatctgag cctgggagct
     6361 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     6421 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     6481 gtcagtgtgg aaaatctcta gcagggccgc tttaaacccg ctgatcagcc tcgactgtgc
     6541 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     6601 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     6661 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     6721 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca
     6781 gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     6841 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     6901 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     6961 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     7021 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     7081 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     7141 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     7201 atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg
     7261 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     7321 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     7381 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     7441 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     7501 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     7561 cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcagca
     7621 cgtgttgaca attaatcatc ggcatagtat atcggcatag tataatacga caaggtgagg
     7681 aactaaacca tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc
     7741 ggagcggtcg agttctggac cgaccggctc gggttctccc gggacttcgt ggaggacgac
     7801 ttcgccggtg tggtccggga cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg
     7861 gtgccggaca acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag
     7921 tggtcggagg tcgtgtccac gaacttccgg gacgcctccg ggccggccat gaccgagatc
     7981 ggcgagcagc cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac
     8041 ttcgtggccg aggagcagga ctgacacgtg ctacgagatt tcgattccac cgccgccttc
     8101 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
     8161 ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt
     8221 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     8281 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct
     8341 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     8401 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     8461 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     8521 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     8581 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     8641 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     8701 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     8761 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     8821 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     8881 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     8941 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     9001 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     9061 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     9121 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     9181 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     9241 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     9301 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     9361 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     9421 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     9481 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     9541 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     9601 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     9661 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     9721 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     9781 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     9841 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     9901 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     9961 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
    10021 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
    10081 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
    10141 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
    10201 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
    10261 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
    10321 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
    10381 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
    10441 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
    10501 cgaaaagtgc cacctgacgt c
//
