LOCUS       sequence-275419-       10504 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivector encoding 3XHA-tagged GK (isoform 1).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10504)
  AUTHORS   Zhang D, Tomisato W, Su L, Sun L, Choi JH, Zhang Z, Wang KW, Zhan X,
            Choi M, Li X, Tang M, Castro-Perez JM, Hildebrand S, Murray AR, 
            Moresco EMY, Beutler B
  TITLE     Skin-specific regulation of SREBP processing and lipid biosynthesis 
            by glycerol kinase 5.
  JOURNAL   Proc Natl Acad Sci U S A. 2017 Jun 27;114(26):E5197-E5206. doi: 
            10.1073/pnas.1705312114. Epub 2017 Jun 12.
  PUBMED    28607088
REFERENCE   2  (bases 1 to 10504)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1705312114"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2017-06-27- 27"; volume: "114"; issue: "26"; pages: 
            "E5197-E5206"
COMMENT     Sequence Label: pBOB-N-3HA-GK_v1
FEATURES             Location/Qualifiers
     source          1..10504
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1059..1184
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1677..1910
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2095..2139
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2391..2507
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2530..2833
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2834..3037
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3034..3058
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3136..3162
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3169..3195
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3199..3225
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     intron          4905..5134
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     5117..5136
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    5311..5899
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5364..5384)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5782..5793)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6306..6486
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6512..6529)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6518..6742
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6788..7216
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6875..6894)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7085..7106
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7225..7245)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7230..7559
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7410..7545
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7472..7491
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7607..7654
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7673..8047
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8177..8298
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8214..8233)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8268..8287
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8347..8363)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8347..8363)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8360..8382)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8371..8387
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8395..8401,8402..8419,8420..8425))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8440..8461
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8578..8595)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8749..9337)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8829..8848)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9508..10299,10300..10368))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10131..10150
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10369..10473)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     1081 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     1141 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     1201 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     1261 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     1321 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     1381 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     1441 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     1501 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     1561 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1621 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1681 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1741 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1801 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1861 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1921 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1981 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     2041 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     2101 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     2161 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     2221 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     2281 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     2341 ggtggagaga gagacagaga cagatccatt cgattagtga acggatcaac ttttaaaaga
     2401 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
     2461 atacaaacta aagaattaca aaaacaaatt acaaaattca aaattttatc gataagcttg
     2521 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     2581 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     2641 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     2701 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     2761 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     2821 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
     2881 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     2941 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
     3001 taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc
     3061 ctggagacgc catccacgct gttttgacct ccatagaaga caccgactga gctccttaag
     3121 gttaacgcca ccatgtaccc ttatgacgta cctgactatg ctggcgccta cccttatgac
     3181 gtacctgact atgctggata cccttatgac gtacctgact atgctggatc tagagaattc
     3241 ggatccatgg cagccgcgaa gaaagcagtt ctggggccat tggtgggagc agtggaccag
     3301 ggtaccagct cgacacgttt tttggttttc aattcaaaaa cagctgaact tcttagtcat
     3361 catcaagtag aaataaaaca ggaattccca agagaaggat gggtagaaca agacccgaag
     3421 gaaattctgc agtctgttta tgagtgtata gagaaaacgt gtgagaaact tggacagctc
     3481 aatattgata tttccaacat caaagccatt ggtgtcagca accagaggga aaccacagta
     3541 gtctgggaca aggtcaccgg agagcctctc tataatgccg tggtgtggct tgacctaaga
     3601 acccagtcta ctgttgagaa ccttagtaaa agaattccag gaaataataa ctttgtcaag
     3661 tccaagacag gccttccact tagcacgtat ttcagtgcag tgaaacttcg ttggctcctt
     3721 gacaacgtga aaaaggtcca agaggctgtt gaagaaaata gagctctttt tgggaccatt
     3781 gattcatggc ttatttggag tttaacagga ggaatccatg ggggtgtcca ctgtacagat
     3841 gtaacaaatg caagcaggac gatgcttttt aacattcatt ctttggaatg ggataaagag
     3901 ctctgcgaat tttttggaat tccaatggaa attcttccca acgttcggag ttcttctgag
     3961 atctatggcc taatgaaagc tggggccttg gaaggtgtac caatatctgg gtgtttgggg
     4021 gaccagtctg ctgctttggt gggacaaatg tgcttccagg atggacaggc caaaaacacg
     4081 tatggaacag ggtgcttctt attgtgcaac acgggccata agtgtgtatt ttctgaacat
     4141 ggcctcctga caaccgtagc atataaactt ggcagagaca aacctgtgta ttatgcgttg
     4201 gaaggttccg tggctatagc tggtgctgta atccgctggc taagagacaa ccttggaatt
     4261 attaagtcct ctgaggaaat tgaaaaactt gctaaggaag taggtacttc ttatggctgc
     4321 tacttcgttc cagcattttc agggttatat gcgccttatt gggagcccag tgcaagaggg
     4381 atcatctgtg gactcactca gttcaccaat aaatgtcata tcgcttttgc tgcactagaa
     4441 gctgtttgtt tccaaacccg agagattttg gatgccatga atcgcgactg tggaattcca
     4501 ctcagtcatt tacaggtaga tggaggaatg accagcaata aaattcttat gcagctacaa
     4561 gcagacattc tgtatattcc agtagtgaaa ccctccatgc ctgaaacaac tgcactaggc
     4621 gctgccatgg cagctggggc tgcagagggg gttggtgtgt ggagtcttga acctgaggat
     4681 ttgtcagctg tcacaatgga gcggtttgaa cctcagatca atgctgaaga aagcgaaatc
     4741 cgttactcca catggaagaa agctgtgatg aagtcaattg gttgggttac aactcagtct
     4801 ccagaaagtg gtatcccact cgagccatgg aagcttgata tctaactgac tgaaccggtg
     4861 gtaccgagga attaattcgc tgtctgcgag ggccagctgt tggggtgagt actccctctc
     4921 aaaagcgggc atgacttctg cgctaagatt gtcagtttcc aaaaacgagg aggatttgat
     4981 attcacctgg cccgcggtga tgcctttgag ggtggccgcg tccatctggt cagaaaagac
     5041 aatctttttg ttgtcaagct tgaggtgtgg caggcttgag atctggccat acacttgagt
     5101 gacaatgaca tccactttgc ctttctctcc acaggtgtcc actcccaggt ccaactgcag
     5161 gtcgagcatg catctagggc ggccaattcc gccgatcctg gaaaaacatg gagcaatcac
     5221 aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac aagaggagga
     5281 ggaggtgggt tttccagtca cacctcagac aatcaacctc tggattacaa aatttgtgaa
     5341 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     5401 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     5461 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     5521 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     5581 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     5641 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     5701 gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg
     5761 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     5821 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     5881 ctttgggccg cctccccgcc tggaattcga gctcggtacc tttaagacca atgacttaca
     5941 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
     6001 actcccaacg aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc
     6061 ctgattggca gaactacaca ccagggccag ggatcagata tccactgacc tttggatggt
     6121 gctacaagct agtaccagtt gagcaagaga aggtagaaga agccaatgaa ggagagaaca
     6181 cccgcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtattag
     6241 agtggaggtt tgacagccgc ctagcatttc atcacatggc ccgagagctg catccggact
     6301 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     6361 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     6421 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     6481 ctagcagggc cgctttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca
     6541 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     6601 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     6661 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     6721 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg
     6781 tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     6841 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     6901 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     6961 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     7021 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     7081 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     7141 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     7201 aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     7261 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     7321 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     7381 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     7441 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg
     7501 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     7561 agctcccggg agcttgtata tccattttcg gatctgatca gcacgtgttg acaattaatc
     7621 atcggcatag tatatcggca tagtataata cgacaaggtg aggaactaaa ccatggccaa
     7681 gttgaccagt gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg
     7741 gaccgaccgg ctcgggttct cccgggactt cgtggaggac gacttcgccg gtgtggtccg
     7801 ggacgacgtg accctgttca tcagcgcggt ccaggaccag gtggtgccgg acaacaccct
     7861 ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc
     7921 cacgaacttc cgggacgcct ccgggccggc catgaccgag atcggcgagc agccgtgggg
     7981 gcgggagttc gccctgcgcg acccggccgg caactgcgtg cacttcgtgg ccgaggagca
     8041 ggactgacac gtgctacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
     8101 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
     8161 gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     8221 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     8281 actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta
     8341 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     8401 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     8461 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     8521 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     8581 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     8641 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     8701 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     8761 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     8821 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     8881 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     8941 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     9001 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     9061 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     9121 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     9181 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     9241 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     9301 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     9361 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     9421 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     9481 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     9541 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     9601 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     9661 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     9721 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     9781 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     9841 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     9901 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     9961 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
    10021 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
    10081 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
    10141 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
    10201 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
    10261 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
    10321 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
    10381 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
    10441 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
    10501 cgtc
//
