LOCUS       sequence-27543-e         724 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 724)
  AUTHORS   Evers TH, van Dongen EM, Faesen AC, Meijer EW, Merkx M
  TITLE     Quantitative understanding of the energy transfer between 
            fluorescent proteins connected via flexible peptide linkers.
  JOURNAL   Biochemistry. 2006 Nov 7. 45(44):13183-92.
  PUBMED    17073440
REFERENCE   2  (bases 1 to 724)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2006 Nov 7. 45(44):13183-92."
COMMENT     Sequence Label: pET28CLY5
FEATURES             Location/Qualifiers
     source          1..724
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     99..120
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(313..334)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     662..681
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gggcagcttg ccggnggtgc agatgaactt cagggtcagc ttgccgtagg tggcatcgcc
       61 ctcgccctcg ccggacacgc tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag
      121 gatgggcacc accccggtga acagctcctc gcccttgctc accatggtgc tacctccgga
      181 tccaccacta ccaccggatc caccagaacc tccggatcca ccactaccac cggatccacc
      241 tgatccaccg gatccaccac gaattccgga cttgtacagc tcgtccatgc cgagagtgat
      301 cccggcggcg gtcacgaact ccagcaggac catgtgatcg cgcttctcgt tggggtcttt
      361 gctcagggcg gactgggtgc tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc
      421 gatgggggtg ttctgctggt agtggtcggc gagctgcacg ctgccgtcct cgatgttgtg
      481 gcggatcttg aagttggcct tgatgccgtt cttctgcttg tcggcggtga tatagacgtt
      541 gtggctgatg tagttgtact ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc
      601 gatgcccttc agctcgatgc ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg
      661 ggtcttgtag ttgccgtcgt ccttgaagaa gatggtgcgc tcctggacgt agccttcggg
      721 catg
//
