LOCUS       sequence-275430-        8915 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Myc-tagged SCAP in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8915)
  AUTHORS   Zhang D, Tomisato W, Su L, Sun L, Choi JH, Zhang Z, Wang KW, Zhan X,
            Choi M, Li X, Tang M, Castro-Perez JM, Hildebrand S, Murray AR, 
            Moresco EMY, Beutler B
  TITLE     Skin-specific regulation of SREBP processing and lipid biosynthesis 
            by glycerol kinase 5.
  JOURNAL   Proc Natl Acad Sci U S A. 2017 Jun 27;114(26):E5197-E5206. doi: 
            10.1073/pnas.1705312114. Epub 2017 Jun 12.
  PUBMED    28607088
REFERENCE   2  (bases 1 to 8915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1705312114"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2017-06-27- 27"; volume: "114"; issue: "26"; pages: 
            "E5197-E5206"
COMMENT     Sequence Label: pcDNA6-N-Myc-SCAP
FEATURES             Location/Qualifiers
     source          1..8915
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      921..930
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             930..959
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(4871..4888)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4877..5101
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5147..5575
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5234..5253)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5444..5465
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5584..5604)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5589..5918
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5769..5904
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5831..5850
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5966..6013
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6032..6430
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    6588..6709
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6625..6644)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6679..6698
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6758..6774)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6758..6774)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6771..6793)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6782..6798
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6806..6812,6813..6830,6831..6836))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6851..6872
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6989..7006)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7160..7748)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7240..7259)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7919..8710,8711..8779))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8542..8561
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8780..8884)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt
      901 tagagctcct taaggttaac gccaccatgg aacaaaaact catctcagaa gaggatctgt
      961 ctagagaatt cggatccatg accctgactg aaaggcttcg tgagaagata tctcaggcct
     1021 tctacaacca tgggctgctc tgcgcatcct atccaattcc catcatcctc ttcacaggac
     1081 tctgcatctt agcctgctgc tacccgctgc tgaagctccc cttgcctgga acgggacctg
     1141 tggaattctc cacgcctgtg aagggttact cgcccccgcc tgcggactct gaccacaaac
     1201 aaggagagcc cagcgagcag ccagagtggt atgtgggtgc ccccgtggcg tacatccaac
     1261 agatatttgt gaagtcatcg gtgtctccct ggcacagaaa tcttctggca gtcgatgtgt
     1321 tccggtcacc tctgtcccga gcattccaac tggtggaaga gatccggaac catgtgctga
     1381 gagacagctc agggaccaag agcctggagg aggtttgcct gcaggtgaca gacctgctgc
     1441 caggcctcag gaaactccgg agcctacttc ccgaacatgg ctgcctgctg ctgtcccctg
     1501 ggaacttctg gcagaatgat tgggagagat tccatgccga ccctgacatc attgggacca
     1561 tccatcaaca tgagcccaaa actctacaga catcagccac actcaaagac ttgctgtttg
     1621 gtgttcctgg gaagtacagt ggggtgagcc tctacacaag gaaaaggatg gtctcctaca
     1681 ccatcaccct ggtcttccag cgctaccatg ccaagtttct gagcagccta cgtgcccggc
     1741 tcatgctgct gcaccccagc cccaactgca gcctccgagc agagaacctg gtccacgtcc
     1801 acttcaaaga ggagattggc attgctgagc tcatcccgct cgtgaccacc tacatcatcc
     1861 tgtttgccta catctacttc tccacacgca agatcgacat ggtcaagtcc aagtggggcc
     1921 tcgccctggc agccgtggtc acagtactta gctcactgct catgtctgtg gggctctgca
     1981 ccctcttcgg cctgacgccc acactcaatg gcggtgagat cttcccatac ctggtggtcg
     2041 ttattgggct agagaacgtg ttggtgctca ccaagtcagt ggtatcaact ccagtggacc
     2101 tcgaggtgaa gcttcggatt gcacaaggct tgagtagtga gagctggtcc atcatgaaga
     2161 acgcggcgac cgagctgggc atcatcctca ttggctactt caccctcgtg cctgctatcc
     2221 aggagttctg cctctttgct gttgtgggcc tggtgtctga cttcttcctc cagatgctgt
     2281 tcttcaccac tgtcctgtcg atcgacattc gccggatgga gctagcagac ctaaacaagc
     2341 ggctgccccc tgaatcctgc ctgccctcag ccaagcccgt ggggaggcca gcacgatatg
     2401 agagacagca ggctgtacgg ccatccacgc cacacaccat cacattgcaa ccatcttcct
     2461 tccgaaacct gcggcttccc aaaaggctgc gtgtcatcta cttcctggcc cgcactcgcc
     2521 tggcccagcg cctcatcatg gctggtaccg ttgtctggat tggcatcctg gtatacaccg
     2581 acccggcagg gctgcgcacc taccttgctg cccaggtgac agagcagagc ccactgggtg
     2641 agggttccct gggccccatg cctgtgccta gcggagtgct gcctgccagc cacccggacc
     2701 ctgcattctc catcttccca cctgatgctc ctaaactgcc agagaaccag accttgccag
     2761 gtgagctgcc tgagcatgct ggtccagcag agggtgtcca tgacagccga gccccagagg
     2821 taacttgggg gcctgaggat gaggagctgt ggaggaaatt gtccttccgc cactggccca
     2881 cactcttcaa ctactacaac atcacactgg ccaaaaggta catcagcctg ctgcctgtca
     2941 tccctgtcac actacacctg aatccacggg aggctctgga ggggcgacac cctcaggatg
     3001 gtcgcagtgc ctgggcccca caagagcctt tgcccgctgg cctctgggag tccggaccta
     3061 agggaccagg tggaacacag acccatggcg acattacctt gtacaaggtg gccgcgcttg
     3121 gcctagcagc gggcatcgtc ctggtgctgc tgctgctctg cctctaccgg gtgctctgcc
     3181 cgcgtaatta tgggcagccg ggtggtggcc ccggcaggcg gaggcgcggg gagctgccct
     3241 gcgatgacta cggctacgca ccgcccgaga cggagatagt gccgctggtg ctgcgaggtc
     3301 acctcatgga catcgagtgt ctggctagcg atgggatgct actagtgagc tgctgcctgg
     3361 caggccaagt ctgcgtgtgg gacgctcaga caggggactg cctcacacgg atcccacgcc
     3421 cagggccacg ccgggatagc tgcggaggtg gagcttttga gactcaggag aactgggaaa
     3481 ggctgtcaga tggaggcaag gctagcccgg aagaacctgg agacagccct ccgctgcgac
     3541 gacgcccccg agggcctcca ccgccttccc tctttgggga ccagcccgac ctcacctgct
     3601 taatcgacac caacttctcg gtgcagctgc ccccagagcc cactcagccc gagcctcggc
     3661 accgggtggg ctgtggccgc tctagagact cgggttatga cttcagccgc ctggtgcagc
     3721 gtgtgtacca ggaggaaggc ctggctgcta tgcgcatgcc ggccctgcgc ccaccctccc
     3781 ctggacctcc cttgccccag gcctctcaag aagaggggac tgcacctgag aagggctccc
     3841 ctcccctggc ctggaccccc agcacagccg gttccatctg gagcttagag ctgcaaggca
     3901 atctcatcgt ggttgggcgg agcagcggcc ggctggaggt gtgggacgcc attgaggggg
     3961 tgctctgctg cagcaatgag gagatctcct caggcatcac agcccttgtc ttcttggaca
     4021 ggaggattgt agctgctcgg cttaatggtt cccttgattt cttttctttg gagacccaca
     4081 cttccctcag ccccctgcag ttcagaggga ccccagggcg aggcagttct ccttcctcat
     4141 ctgtgtacag cagcagcaac acagtgacct gtcatcggac ccacacagtg ccctgtgcac
     4201 accagaagcc catcacagcc ctgagagctg ctgccgggcg cctagtgaca gggagccaag
     4261 accatactct aagagtcttc cgactggatg actcgtgttg cctctttacc ctgaagggcc
     4321 actcaggggc aatcacagct gtgtacattg atcagaccat ggtactggcc agtggaggac
     4381 aagatggagc catctgcctg tgggatgtac taacaggcag ccgggtcagc caaacatttg
     4441 ctcaccgtgg agatgttacc tccctcacct gtaccgcttc ctgtgtcatt agtagtggcc
     4501 tggatgactt catcagtatc tgggaccgca gcacaggcat caagctgtac tccattcagc
     4561 aggacctggg ctgtggtgca agcttgggtg tcatctcaga taaccttctg gtgaccggcg
     4621 gccagggctg tgtctccttt tgggacctaa actatgggga cctgttacag acagtctact
     4681 tgggcaagaa cagtgaagcc cagcctgccc ggcagatttt ggtgttggac aatgctgcca
     4741 ttgtctgcaa ctttggcagt gagctcagcc tagtgtatgt gccctctgtg ctggagaaac
     4801 tggactgact cgagccatgg aagcttgata tctaactgac tgaaccggtg gtaccaaacc
     4861 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     4921 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     4981 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     5041 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
     5101 gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc
     5161 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     5221 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     5281 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     5341 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     5401 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     5461 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     5521 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc
     5581 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
     5641 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     5701 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     5761 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     5821 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt
     5881 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat
     5941 ccattttcgg atctgatcag cacgtgttga caattaatca tcggcatagt atatcggcat
     6001 agtataatac gacaaggtga ggaactaaac catggccaag cctttgtctc aagaagaatc
     6061 caccctcatt gaaagagcaa cggctacaat caacagcatc cccatctctg aagactacag
     6121 cgtcgccagc gcagctctct ctagcgacgg ccgcatcttc actggtgtca atgtatatca
     6181 ttttactggg ggaccttgtg cagaactcgt ggtgctgggc actgctgctg ctgcggcagc
     6241 tggcaacctg acttgtatcg tcgcgatcgg aaatgagaac aggggcatct tgagcccctg
     6301 cggacggtgc cgacaggtgc ttctcgatct gcatcctggg atcaaagcca tagtgaagga
     6361 cagtgatgga cagccgacgg cagttgggat tcgtgaattg ctgccctctg gttatgtgtg
     6421 ggagggctaa gcacttcgtg gccgaggagc aggactgaca cgtgctacga gatttcgatt
     6481 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
     6541 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg
     6601 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     6661 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta
     6721 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     6781 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     6841 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     6901 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     6961 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     7021 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     7081 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     7141 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     7201 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     7261 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     7321 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     7381 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     7441 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     7501 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     7561 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     7621 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     7681 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     7741 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     7801 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     7861 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     7921 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     7981 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     8041 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     8101 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     8161 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     8221 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     8281 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     8341 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     8401 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     8461 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     8521 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     8581 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     8641 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     8701 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     8761 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     8821 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     8881 cgcgcacatt tccccgaaaa gtgccacctg acgtc
//
